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P27747

- ACOC_CUPNH

UniProt

P27747 - ACOC_CUPNH

Protein

Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving system

Gene

acoC

Organism
Cupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Dihydrolipoamide acetyltransferase involved in acetoin catabolism.

    Catalytic activityi

    Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine.

    Cofactori

    Binds 1 lipoyl cofactor covalently.By similarity

    Pathwayi

    GO - Molecular functioni

    1. dihydrolipoyllysine-residue acetyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. acetoin catabolic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Acetoin catabolism

    Enzyme and pathway databases

    BioCyciCNEC381666:GJUJ-3857-MONOMER.
    RETL1328306-WGS:GSTH-5096-MONOMER.
    RETL1328306-WGS:GSTH-5109-MONOMER.
    UniPathwayiUPA00040.

    Protein family/group databases

    MEROPSiS33.010.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving system (EC:2.3.1.12)
    Alternative name(s):
    Acetoin dehydrogenase E2 component
    Dihydrolipoamide acetyltransferase component of acetoin cleaving system
    Fast-migrating protein
    Short name:
    FMP
    Gene namesi
    Name:acoC
    Ordered Locus Names:H16_B0146
    OrganismiCupriavidus necator (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) (Ralstonia eutropha)
    Taxonomic identifieri381666 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaBetaproteobacteriaBurkholderialesBurkholderiaceaeCupriavidus
    ProteomesiUP000008210: Chromosome 2

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 374373Dihydrolipoyllysine-residue acetyltransferase component of acetoin cleaving systemPRO_0000162304Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei50 – 501N6-lipoyllysineBy similarity

    Expressioni

    Inductioni

    By growth on acetoin.

    Interactioni

    Protein-protein interaction databases

    STRINGi381666.H16_B0146.

    Structurei

    3D structure databases

    ProteinModelPortaliP27747.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini10 – 8374Lipoyl-bindingAdd
    BLAST

    Sequence similaritiesi

    Contains 1 lipoyl-binding domain.Curated

    Keywords - Domaini

    Lipoyl

    Phylogenomic databases

    eggNOGiCOG0508.
    HOGENOMiHOG000261089.
    KOiK00627.
    OMAiAYETPAD.
    OrthoDBiEOG64FKGZ.

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000089. Biotin_lipoyl.
    IPR011053. Single_hybrid_motif.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    [Graphical view]
    PRINTSiPR00111. ABHYDROLASE.
    SUPFAMiSSF51230. SSF51230. 1 hit.
    SSF53474. SSF53474. 1 hit.
    PROSITEiPS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00189. LIPOYL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27747-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATEISPTII PIVMPKWGLS MKEGTVNAWL VDEGTEITVG LPILDVETDK    50
    IANAVEAPDA GTLRRKVAQA GDVLPVKALL GVLAPAEVSD AQIDDYVAAY 100
    ETPADDAGEE DAAAAYQFAD VDGIRVRYAR KGGGAETVLF IHGFGGDLDN 150
    WLFNLDPLAD AYTVVALDLP GHGQSSPRLA GTTLAQMAGF VARFMDETGI 200
    EAAHVVGHSM GGGVAAQLAV DAPQRVLSVA LVSPVGFGDA VNSGYTEGFV 250
    SAQSRRELKP VVELLFADAG LVSRQMLDDL LRYKRLDGVT EALTALGQGL 300
    FGGGRQSEQP GQRLANSGKR VLVVWGGQDQ IIPAAHAEAA PPGATVKVFA 350
    DAGHMSQMEK ANDFNALLKK HLGG 374
    Length:374
    Mass (Da):39,135
    Last modified:January 23, 2007 - v3
    Checksum:i0EBD42C03A81A189
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951D → G in AAA21950. (PubMed:2061286)Curated
    Sequence conflicti121 – 1211V → G in AAA21950. (PubMed:2061286)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M66060 Genomic DNA. Translation: AAA21950.1.
    AM260480 Genomic DNA. Translation: CAJ94952.1.
    RefSeqiYP_728317.1. NC_008314.1.

    Genome annotation databases

    EnsemblBacteriaiCAJ94952; CAJ94952; H16_B0146.
    GeneIDi4454951.
    KEGGireh:H16_B0146.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M66060 Genomic DNA. Translation: AAA21950.1 .
    AM260480 Genomic DNA. Translation: CAJ94952.1 .
    RefSeqi YP_728317.1. NC_008314.1.

    3D structure databases

    ProteinModelPortali P27747.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 381666.H16_B0146.

    Protein family/group databases

    MEROPSi S33.010.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAJ94952 ; CAJ94952 ; H16_B0146 .
    GeneIDi 4454951.
    KEGGi reh:H16_B0146.

    Phylogenomic databases

    eggNOGi COG0508.
    HOGENOMi HOG000261089.
    KOi K00627.
    OMAi AYETPAD.
    OrthoDBi EOG64FKGZ.

    Enzyme and pathway databases

    UniPathwayi UPA00040 .
    BioCyci CNEC381666:GJUJ-3857-MONOMER.
    RETL1328306-WGS:GSTH-5096-MONOMER.
    RETL1328306-WGS:GSTH-5109-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR003016. 2-oxoA_DH_lipoyl-BS.
    IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR000089. Biotin_lipoyl.
    IPR011053. Single_hybrid_motif.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    PF00364. Biotin_lipoyl. 1 hit.
    [Graphical view ]
    PRINTSi PR00111. ABHYDROLASE.
    SUPFAMi SSF51230. SSF51230. 1 hit.
    SSF53474. SSF53474. 1 hit.
    PROSITEi PS50968. BIOTINYL_LIPOYL. 1 hit.
    PS00189. LIPOYL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and molecular characterization of the Alcaligenes eutrophus H16 aco operon genes involved in acetoin catabolism."
      Priefert H., Hein S., Krueger N., Zeh K., Schmidt B., Steinbuechel A.
      J. Bacteriol. 173:4056-4071(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-28.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 17699 / H16 / DSM 428 / Stanier 337.

    Entry informationi

    Entry nameiACOC_CUPNH
    AccessioniPrimary (citable) accession number: P27747
    Secondary accession number(s): Q0K4X2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3