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P27744

- IPNS_AMYLA

UniProt

P27744 - IPNS_AMYLA

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Protein

Isopenicillin N synthase

Gene

pcbC

Organism
Amycolatopsis lactamdurans (Nocardia lactamdurans)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.

Catalytic activityi

N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.

Cofactori

Iron.
Ascorbate.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi210 – 2101IronPROSITE-ProRule annotation
Metal bindingi212 – 2121IronPROSITE-ProRule annotation
Metal bindingi266 – 2661IronPROSITE-ProRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. isopenicillin-N synthase activity Source: UniProtKB-EC
  3. L-ascorbic acid binding Source: UniProtKB-KW
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13365.
UniPathwayiUPA00149; UER00240.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopenicillin N synthase (EC:1.21.3.1)
Short name:
IPNS
Gene namesi
Name:pcbC
OrganismiAmycolatopsis lactamdurans (Nocardia lactamdurans)
Taxonomic identifieri1913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeAmycolatopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Isopenicillin N synthasePRO_0000219502Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP27744.
SMRiP27744. Positions 6-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 284107Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27744-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKMPSAEVPT IDVSPLFGDD AQEKVRVGQE INKACRGSGF FYAANHGVDV
60 70 80 90 100
QRLQDVVNEF HRTMSPQEKY DLAIHAYNKN NSHVRNGYYM AIEGKKAVES
110 120 130 140 150
FCYLNPSFSE DHPEIKAGTP MHEVNSWPDE EKHPSFRPFC EEYYWTMHRL
160 170 180 190 200
SKVLMRGFAL ALGKDERFFE PELKEADTLS SVSLIRYPYL EDYPPVKTGP
210 220 230 240 250
DGEKLSFEDH FDVSMITVLY QTQVQNLQVE TVDGWRDLPT SDTDFLVNAG
260 270 280 290 300
TYLGHLTNDY FPSPLHRVKF VNAERLSLPF FFHAGQHTLI EPFFPDGAPE
310 320
GKQGNEAVRY GDYLNHGLHS LIVKNGQT
Length:328
Mass (Da):37,466
Last modified:August 1, 1992 - v1
Checksum:iF0DE8B0727AC3855
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57310 Genomic DNA. Translation: CAA40562.1.
PIRiS15284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X57310 Genomic DNA. Translation: CAA40562.1 .
PIRi S15284.

3D structure databases

ProteinModelPortali P27744.
SMRi P27744. Positions 6-328.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00149 ; UER00240 .
BioCyci MetaCyc:MONOMER-13365.

Family and domain databases

Gene3Di 2.60.120.330. 1 hit.
InterProi IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view ]
Pfami PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view ]
PRINTSi PR00682. IPNSYNTHASE.
PROSITEi PS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum."
    Coque J.J.R., Martin J.F., Calzada J.G., Liras P.
    Mol. Microbiol. 5:1125-1133(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VAR LC 411.

Entry informationi

Entry nameiIPNS_AMYLA
AccessioniPrimary (citable) accession number: P27744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 1, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3