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Protein

Isopenicillin N synthase

Gene

pcbC

Organism
Amycolatopsis lactamdurans (Nocardia lactamdurans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.

Catalytic activityi

N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi210 – 2101IronPROSITE-ProRule annotation
Metal bindingi212 – 2121IronPROSITE-ProRule annotation
Metal bindingi266 – 2661IronPROSITE-ProRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. isopenicillin-N synthase activity Source: UniProtKB-EC
  3. L-ascorbic acid binding Source: UniProtKB-KW
  4. oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Source: InterPro

GO - Biological processi

  1. antibiotic biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Antibiotic biosynthesis

Keywords - Ligandi

Iron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13365.
UniPathwayiUPA00149; UER00240.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopenicillin N synthase (EC:1.21.3.1)
Short name:
IPNS
Gene namesi
Name:pcbC
OrganismiAmycolatopsis lactamdurans (Nocardia lactamdurans)
Taxonomic identifieri1913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeAmycolatopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 328328Isopenicillin N synthasePRO_0000219502Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP27744.
SMRiP27744. Positions 6-328.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini178 – 284107Fe2OG dioxygenasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Fe2OG dioxygenase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMPSAEVPT IDVSPLFGDD AQEKVRVGQE INKACRGSGF FYAANHGVDV
60 70 80 90 100
QRLQDVVNEF HRTMSPQEKY DLAIHAYNKN NSHVRNGYYM AIEGKKAVES
110 120 130 140 150
FCYLNPSFSE DHPEIKAGTP MHEVNSWPDE EKHPSFRPFC EEYYWTMHRL
160 170 180 190 200
SKVLMRGFAL ALGKDERFFE PELKEADTLS SVSLIRYPYL EDYPPVKTGP
210 220 230 240 250
DGEKLSFEDH FDVSMITVLY QTQVQNLQVE TVDGWRDLPT SDTDFLVNAG
260 270 280 290 300
TYLGHLTNDY FPSPLHRVKF VNAERLSLPF FFHAGQHTLI EPFFPDGAPE
310 320
GKQGNEAVRY GDYLNHGLHS LIVKNGQT
Length:328
Mass (Da):37,466
Last modified:August 1, 1992 - v1
Checksum:iF0DE8B0727AC3855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57310 Genomic DNA. Translation: CAA40562.1.
PIRiS15284.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57310 Genomic DNA. Translation: CAA40562.1.
PIRiS15284.

3D structure databases

ProteinModelPortaliP27744.
SMRiP27744. Positions 6-328.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00149; UER00240.
BioCyciMetaCyc:MONOMER-13365.

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamiPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSiPR00682. IPNSYNTHASE.
PROSITEiPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum."
    Coque J.J.R., Martin J.F., Calzada J.G., Liras P.
    Mol. Microbiol. 5:1125-1133(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: VAR LC 411.

Entry informationi

Entry nameiIPNS_AMYLA
AccessioniPrimary (citable) accession number: P27744
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.