P27744 (IPNS_NOCLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Isopenicillin N synthase
Short name=IPNS
EC=1.21.3.1

Gene names

Name:

pcbC

Organism

Nocardia lactamdurans

Taxonomic identifier

1913 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	328 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.
Catalytic activity	N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.
Cofactor	Iron. Ascorbate.
Pathway	Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Metal-binding Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW iron ion binding Inferred from electronic annotation. Source: InterPro isopenicillin-N synthase activity Inferred from electronic annotation. Source: EC oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 328	328	Isopenicillin N synthase		PRO_0000219502
Regions
	Domain	178 – 284	107	Fe2OG dioxygenase
Sites
	Metal binding	210	1	Iron By similarity
	Metal binding	212	1	Iron By similarity
	Metal binding	266	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27744 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: F0DE8B0727AC3855	FASTA	328	37,466
	10 20 30 40 50 60 MKMPSAEVPT IDVSPLFGDD AQEKVRVGQE INKACRGSGF FYAANHGVDV QRLQDVVNEF 70 80 90 100 110 120 HRTMSPQEKY DLAIHAYNKN NSHVRNGYYM AIEGKKAVES FCYLNPSFSE DHPEIKAGTP 130 140 150 160 170 180 MHEVNSWPDE EKHPSFRPFC EEYYWTMHRL SKVLMRGFAL ALGKDERFFE PELKEADTLS 190 200 210 220 230 240 SVSLIRYPYL EDYPPVKTGP DGEKLSFEDH FDVSMITVLY QTQVQNLQVE TVDGWRDLPT 250 260 270 280 290 300 SDTDFLVNAG TYLGHLTNDY FPSPLHRVKF VNAERLSLPF FFHAGQHTLI EPFFPDGAPE 310 320 GKQGNEAVRY GDYLNHGLHS LIVKNGQT

References

[1]

"The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum." Coque J.J.R., Martin J.F., Calzada J.G., Liras P. Mol. Microbiol. 5:1125-1133(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VAR LC 411.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X57310 Genomic DNA. Translation: CAA40562.1.
PIR	S15284.
3D structure databases
ProteinModelPortal	P27744.
SMR	P27744. Positions 6-328.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-13365.
UniPathway	UPA00149; UER00240.
Family and domain databases
InterPro	IPR026992. DIOX_N. IPR002057. Isopenicillin-N_synth_CS. IPR002283. Isopenicillin-N_synthase. IPR005123. Oxoglu/Fe-dep_dioxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. PF14226. DIOX_N. 1 hit. [Graphical view]
PRINTS	PR00682. IPNSYNTHASE.
PROSITE	PS51471. FE2OG_OXY. 1 hit. PS00185. IPNS_1. 1 hit. PS00186. IPNS_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPNS_NOCLA

Accession

Primary (citable) accession number: P27744

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program