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Protein

Isopenicillin N synthase

Gene

pcbC

Organism
Amycolatopsis lactamdurans (Nocardia lactamdurans)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.

Catalytic activityi

N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.

Cofactori

Protein has several cofactor binding sites:

Pathwayi: penicillin G biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. N-(5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine synthase (pcbAB)
  2. Isopenicillin N synthase (pcbC)
  3. no protein annotated in this organism
This subpathway is part of the pathway penicillin G biosynthesis, which is itself part of Antibiotic biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine, the pathway penicillin G biosynthesis and in Antibiotic biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi210IronPROSITE-ProRule annotation1
Metal bindingi212IronPROSITE-ProRule annotation1
Metal bindingi266IronPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAntibiotic biosynthesis
LigandIron, Metal-binding, Vitamin C

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13365.
UniPathwayiUPA00149; UER00240.

Names & Taxonomyi

Protein namesi
Recommended name:
Isopenicillin N synthase (EC:1.21.3.1)
Short name:
IPNS
Gene namesi
Name:pcbC
OrganismiAmycolatopsis lactamdurans (Nocardia lactamdurans)
Taxonomic identifieri1913 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaPseudonocardialesPseudonocardiaceaeAmycolatopsis

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002195021 – 328Isopenicillin N synthaseAdd BLAST328

Structurei

3D structure databases

ProteinModelPortaliP27744.
SMRiP27744.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini178 – 284Fe2OG dioxygenasePROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Family and domain databases

Gene3Di2.60.120.330. 1 hit.
InterProiView protein in InterPro
IPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
PfamiView protein in Pfam
PF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
PROSITEiView protein in PROSITE
PS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.

Sequencei

Sequence statusi: Complete.

P27744-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKMPSAEVPT IDVSPLFGDD AQEKVRVGQE INKACRGSGF FYAANHGVDV
60 70 80 90 100
QRLQDVVNEF HRTMSPQEKY DLAIHAYNKN NSHVRNGYYM AIEGKKAVES
110 120 130 140 150
FCYLNPSFSE DHPEIKAGTP MHEVNSWPDE EKHPSFRPFC EEYYWTMHRL
160 170 180 190 200
SKVLMRGFAL ALGKDERFFE PELKEADTLS SVSLIRYPYL EDYPPVKTGP
210 220 230 240 250
DGEKLSFEDH FDVSMITVLY QTQVQNLQVE TVDGWRDLPT SDTDFLVNAG
260 270 280 290 300
TYLGHLTNDY FPSPLHRVKF VNAERLSLPF FFHAGQHTLI EPFFPDGAPE
310 320
GKQGNEAVRY GDYLNHGLHS LIVKNGQT
Length:328
Mass (Da):37,466
Last modified:August 1, 1992 - v1
Checksum:iF0DE8B0727AC3855
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X57310 Genomic DNA. Translation: CAA40562.1.
PIRiS15284.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiIPNS_AMYLA
AccessioniPrimary (citable) accession number: P27744
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 15, 2017
This is version 82 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families