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P27744 (IPNS_AMYLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isopenicillin N synthase

Short name=IPNS
EC=1.21.3.1
Gene names
Name:pcbC
OrganismAmycolatopsis lactamdurans (Nocardia lactamdurans)
Taxonomic identifier1913 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesPseudonocardineaePseudonocardiaceaeAmycolatopsis

Protein attributes

Sequence length328 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin.

Catalytic activity

N-((5S)-5-amino-5-carboxypentanoyl)-L-cysteinyl-D-valine + O2 = isopenicillin N + 2 H2O.

Cofactor

Iron.

Ascorbate.

Pathway

Antibiotic biosynthesis; penicillin G biosynthesis; penicillin G from L-alpha-aminoadipate and L-cysteine and L-valine: step 2/3.

Sequence similarities

Belongs to the iron/ascorbate-dependent oxidoreductase family.

Contains 1 Fe2OG dioxygenase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 328328Isopenicillin N synthase
PRO_0000219502

Regions

Domain178 – 284107Fe2OG dioxygenase

Sites

Metal binding2101Iron By similarity
Metal binding2121Iron By similarity
Metal binding2661Iron By similarity

Sequences

Sequence LengthMass (Da)Tools
P27744 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: F0DE8B0727AC3855

FASTA32837,466
        10         20         30         40         50         60 
MKMPSAEVPT IDVSPLFGDD AQEKVRVGQE INKACRGSGF FYAANHGVDV QRLQDVVNEF 

        70         80         90        100        110        120 
HRTMSPQEKY DLAIHAYNKN NSHVRNGYYM AIEGKKAVES FCYLNPSFSE DHPEIKAGTP 

       130        140        150        160        170        180 
MHEVNSWPDE EKHPSFRPFC EEYYWTMHRL SKVLMRGFAL ALGKDERFFE PELKEADTLS 

       190        200        210        220        230        240 
SVSLIRYPYL EDYPPVKTGP DGEKLSFEDH FDVSMITVLY QTQVQNLQVE TVDGWRDLPT 

       250        260        270        280        290        300 
SDTDFLVNAG TYLGHLTNDY FPSPLHRVKF VNAERLSLPF FFHAGQHTLI EPFFPDGAPE 

       310        320 
GKQGNEAVRY GDYLNHGLHS LIVKNGQT 

« Hide

References

[1]"The cephamycin biosynthetic genes pcbAB, encoding a large multidomain peptide synthetase, and pcbC of Nocardia lactamdurans are clustered together in an organization different from the same genes in Acremonium chrysogenum and Penicillium chrysogenum."
Coque J.J.R., Martin J.F., Calzada J.G., Liras P.
Mol. Microbiol. 5:1125-1133(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: VAR LC 411.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57310 Genomic DNA. Translation: CAA40562.1.
PIRS15284.

3D structure databases

ProteinModelPortalP27744.
SMRP27744. Positions 6-328.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-13365.
UniPathwayUPA00149; UER00240.

Family and domain databases

Gene3D2.60.120.330. 1 hit.
InterProIPR026992. DIOX_N.
IPR027443. IPNS-like.
IPR002057. Isopenicillin-N_synth_CS.
IPR002283. Isopenicillin-N_synthase.
IPR005123. Oxoglu/Fe-dep_dioxygenase.
[Graphical view]
PfamPF03171. 2OG-FeII_Oxy. 1 hit.
PF14226. DIOX_N. 1 hit.
[Graphical view]
PRINTSPR00682. IPNSYNTHASE.
PROSITEPS51471. FE2OG_OXY. 1 hit.
PS00185. IPNS_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIPNS_AMYLA
AccessionPrimary (citable) accession number: P27744
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways