Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calpain

Gene
N/A
Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calpains are calcium-activated non-lysosomal thiol-proteases.

Catalytic activityi

Broad endopeptidase specificity.

Enzyme regulationi

Activated by free cytoplasmic calcium.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei154 – 1541By similarity
Active sitei313 – 3131By similarity
Active sitei337 – 3371By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi641 – 652121CuratedAdd
BLAST
Calcium bindingi671 – 682122CuratedAdd
BLAST

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 758758CalpainPRO_0000207735Add
BLAST

Expressioni

Developmental stagei

In sporocysts and adult worms.

Interactioni

Protein-protein interaction databases

STRINGi6183.Smp_157500__mRNA.

Structurei

3D structure databases

ProteinModelPortaliP27730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 397299Calpain catalyticPROSITE-ProRule annotationAdd
BLAST
Domaini658 – 69336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini694 – 72936EF-hand 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni398 – 562165Domain IIIAdd
BLAST
Regioni563 – 58220LinkerAdd
BLAST
Regioni583 – 757175Domain IVAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
OrthoDBiEOG091G049E.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRIQIVYSP DENVSGRTNR PGKEVVDPRT GRIIKVKRET PDDYLNVLKP
60 70 80 90 100
IKGPKRMEFN PYLPKTLTPK GYAKFKLMMN VASKQYETLV KRLKTERTLW
110 120 130 140 150
EDPDFPANDK AIGNLPDFRE RIEWKRPHEI NPNAKFFAGG ASRFDIEQGA
160 170 180 190 200
LGDCWLLAVV ASISGYPQLF DQVVPKDQEL KGPEYVGVVR FRFWRFGHWV
210 220 230 240 250
EVLIDDRLPV RQGRNTLVFM HSNDPTEFWS ALLEKAYAKL NGCYAHLSGG
260 270 280 290 300
SQSEAMEDLT GGICLSLELN QKERPSDLID QLKIYAQRCC LMGCSIDSSV
310 320 330 340 350
MEQKMDNGLI GSHAYSLTGV YPVNYRGRTQ WLMRLRNPWG DSHEWKGAWC
360 370 380 390 400
DGSPQWREIS EQEKKNINLS FTADGEFWMS YEDFVTCFSR VEVCHLGLES
410 420 430 440 450
LEYNQNFHGK RRLDEAIFSG QWQRNVNAGG CINNRTTYWT SPQFRITVED
460 470 480 490 500
PDPDDDDNKC SVLIGLMQTD IRKKVGADFQ PIGFMVYNAP DDLNTLLSRA
510 520 530 540 550
QLLTRSPIAK SQFINTREVT AQFRVPPGSY VVIPSTFDPN IEVNFILRVF
560 570 580 590 600
SQTSITEQEL DEDNTNQGLP DDVIEALKLE DTLLDEDQEI EQKFLAIRDP
610 620 630 640 650
KTNAINAVKL GELLNNSTLQ DIPNFQGFNK ELCRSMVASV DNNLTGHVEL
660 670 680 690 700
NEFMDLWIQA KGWKHIFIKH DVDQSGYFSA YEFREALNDA GYHVSNRLIN
710 720 730 740 750
AIINRYQDPG TDKISFEDFM LCMVRLKTAF ETIEAHPKNI EGTSLFSAED

YLRFSVYI
Length:758
Mass (Da):86,863
Last modified:August 1, 1992 - v1
Checksum:i9F5E0C98B6B2AF19
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti128 – 1281H → L in AAA29858 (PubMed:1869543).Curated
Sequence conflicti214 – 2141R → L in AAA29858 (PubMed:1869543).Curated
Sequence conflicti385 – 3873VTC → CYL in AAA29858 (PubMed:1869543).Curated
Sequence conflicti441 – 4411S → N in AAA29858 (PubMed:1869543).Curated
Sequence conflicti755 – 7551S → R (PubMed:1869543).Curated
Sequence conflicti757 – 7571Y → D (PubMed:1869543).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74233 mRNA. Translation: AAA29857.1.
M67499 mRNA. Translation: AAA29858.1.
PIRiA39343.

Genome annotation databases

GeneDBiSmp_214180.1:pep.
Smp_214190.1:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74233 mRNA. Translation: AAA29857.1.
M67499 mRNA. Translation: AAA29858.1.
PIRiA39343.

3D structure databases

ProteinModelPortaliP27730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6183.Smp_157500__mRNA.

Protein family/group databases

MEROPSiC02.023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiSmp_214180.1:pep.
Smp_214190.1:pep.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
OrthoDBiEOG091G049E.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN_SCHMA
AccessioniPrimary (citable) accession number: P27730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 7, 2016
This is version 95 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

This protein binds calcium.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.