Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Calpain

Gene
N/A
Organism
Schistosoma mansoni (Blood fluke)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Calpains are calcium-activated non-lysosomal thiol-proteases.

Catalytic activityi

Broad endopeptidase specificity.

Enzyme regulationi

Activated by free cytoplasmic calcium.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei154By similarity1
Active sitei313By similarity1
Active sitei337By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Calcium bindingi641 – 6521CuratedAdd BLAST12
Calcium bindingi671 – 6822CuratedAdd BLAST12

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Ligandi

Calcium, Metal-binding

Protein family/group databases

MEROPSiC02.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Calpain (EC:3.4.22.-)
Alternative name(s):
Calcium-activated neutral proteinase
Short name:
CANP
OrganismiSchistosoma mansoni (Blood fluke)
Taxonomic identifieri6183 [NCBI]
Taxonomic lineageiEukaryotaMetazoaPlatyhelminthesTrematodaDigeneaStrigeididaSchistosomatoideaSchistosomatidaeSchistosoma

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002077351 – 758CalpainAdd BLAST758

Expressioni

Developmental stagei

In sporocysts and adult worms.

Interactioni

Protein-protein interaction databases

STRINGi6183.Smp_157500__mRNA.

Structurei

3D structure databases

ProteinModelPortaliP27730.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 397Calpain catalyticPROSITE-ProRule annotationAdd BLAST299
Domaini658 – 693EF-hand 1PROSITE-ProRule annotationAdd BLAST36
Domaini694 – 729EF-hand 2PROSITE-ProRule annotationAdd BLAST36

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni398 – 562Domain IIIAdd BLAST165
Regioni563 – 582LinkerAdd BLAST20
Regioni583 – 757Domain IVAdd BLAST175

Sequence similaritiesi

Belongs to the peptidase C2 family.Curated
Contains 1 calpain catalytic domain.PROSITE-ProRule annotation
Contains 2 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
OrthoDBiEOG091G049E.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27730-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGRIQIVYSP DENVSGRTNR PGKEVVDPRT GRIIKVKRET PDDYLNVLKP
60 70 80 90 100
IKGPKRMEFN PYLPKTLTPK GYAKFKLMMN VASKQYETLV KRLKTERTLW
110 120 130 140 150
EDPDFPANDK AIGNLPDFRE RIEWKRPHEI NPNAKFFAGG ASRFDIEQGA
160 170 180 190 200
LGDCWLLAVV ASISGYPQLF DQVVPKDQEL KGPEYVGVVR FRFWRFGHWV
210 220 230 240 250
EVLIDDRLPV RQGRNTLVFM HSNDPTEFWS ALLEKAYAKL NGCYAHLSGG
260 270 280 290 300
SQSEAMEDLT GGICLSLELN QKERPSDLID QLKIYAQRCC LMGCSIDSSV
310 320 330 340 350
MEQKMDNGLI GSHAYSLTGV YPVNYRGRTQ WLMRLRNPWG DSHEWKGAWC
360 370 380 390 400
DGSPQWREIS EQEKKNINLS FTADGEFWMS YEDFVTCFSR VEVCHLGLES
410 420 430 440 450
LEYNQNFHGK RRLDEAIFSG QWQRNVNAGG CINNRTTYWT SPQFRITVED
460 470 480 490 500
PDPDDDDNKC SVLIGLMQTD IRKKVGADFQ PIGFMVYNAP DDLNTLLSRA
510 520 530 540 550
QLLTRSPIAK SQFINTREVT AQFRVPPGSY VVIPSTFDPN IEVNFILRVF
560 570 580 590 600
SQTSITEQEL DEDNTNQGLP DDVIEALKLE DTLLDEDQEI EQKFLAIRDP
610 620 630 640 650
KTNAINAVKL GELLNNSTLQ DIPNFQGFNK ELCRSMVASV DNNLTGHVEL
660 670 680 690 700
NEFMDLWIQA KGWKHIFIKH DVDQSGYFSA YEFREALNDA GYHVSNRLIN
710 720 730 740 750
AIINRYQDPG TDKISFEDFM LCMVRLKTAF ETIEAHPKNI EGTSLFSAED

YLRFSVYI
Length:758
Mass (Da):86,863
Last modified:August 1, 1992 - v1
Checksum:i9F5E0C98B6B2AF19
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti128H → L in AAA29858 (PubMed:1869543).Curated1
Sequence conflicti214R → L in AAA29858 (PubMed:1869543).Curated1
Sequence conflicti385 – 387VTC → CYL in AAA29858 (PubMed:1869543).Curated3
Sequence conflicti441S → N in AAA29858 (PubMed:1869543).Curated1
Sequence conflicti755S → R (PubMed:1869543).Curated1
Sequence conflicti757Y → D (PubMed:1869543).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74233 mRNA. Translation: AAA29857.1.
M67499 mRNA. Translation: AAA29858.1.
PIRiA39343.

Genome annotation databases

GeneDBiSmp_214180.1:pep.
Smp_214190.1:pep.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74233 mRNA. Translation: AAA29857.1.
M67499 mRNA. Translation: AAA29858.1.
PIRiA39343.

3D structure databases

ProteinModelPortaliP27730.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6183.Smp_157500__mRNA.

Protein family/group databases

MEROPSiC02.023.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneDBiSmp_214180.1:pep.
Smp_214190.1:pep.

Phylogenomic databases

eggNOGiKOG0045. Eukaryota.
ENOG410XP0B. LUCA.
OrthoDBiEOG091G049E.

Family and domain databases

CDDicd00214. Calpain_III. 1 hit.
cd00044. CysPc. 1 hit.
cd00051. EFh. 1 hit.
Gene3Di1.10.238.10. 1 hit.
InterProiIPR033883. C2_III.
IPR022684. Calpain_cysteine_protease.
IPR022682. Calpain_domain_III.
IPR022683. Calpain_III.
IPR011992. EF-hand-dom_pair.
IPR002048. EF_hand_dom.
IPR000169. Pept_cys_AS.
IPR001300. Peptidase_C2_calpain_cat.
[Graphical view]
PfamiPF01067. Calpain_III. 1 hit.
PF00648. Peptidase_C2. 1 hit.
[Graphical view]
PRINTSiPR00704. CALPAIN.
SMARTiSM00720. calpain_III. 1 hit.
SM00230. CysPc. 1 hit.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
SSF49758. SSF49758. 1 hit.
PROSITEiPS50203. CALPAIN_CAT. 1 hit.
PS50222. EF_HAND_2. 2 hits.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCAN_SCHMA
AccessioniPrimary (citable) accession number: P27730
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Miscellaneous

This protein binds calcium.

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.