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Protein

Glyceraldehyde-3-phosphate dehydrogenase

Gene

gap

Organism
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the oxidative phosphorylation of glyceraldehyde 3-phosphate (G3P) to 1,3-bisphosphoglycerate (BPG) using the cofactor NAD. The first reaction step involves the formation of a hemiacetal intermediate between G3P and a cysteine residue, and this hemiacetal intermediate is then oxidized to a thioester, with concomitant reduction of NAD to NADH. The reduced NADH is then exchanged with the second NAD, and the thioester is attacked by a nucleophilic inorganic phosphate to produce BPG.By similarity

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.By similarity

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.Curated
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase (gap)
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)
  4. Enolase (eno)
  5. Pyruvate kinase (pykF), Pyruvate kinase (pykA)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei37 – 371NADBy similarity
Binding sitei81 – 811NAD; via carbonyl oxygenBy similarity
Binding sitei123 – 1231NADBy similarity
Active sitei154 – 1541NucleophileBy similarity
Sitei181 – 1811Activates thiol group during catalysisBy similarity
Binding sitei184 – 1841Glyceraldehyde 3-phosphateBy similarity
Binding sitei185 – 1851NADBy similarity
Binding sitei199 – 1991Glyceraldehyde 3-phosphateBy similarity
Binding sitei235 – 2351Glyceraldehyde 3-phosphateBy similarity
Binding sitei314 – 3141NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 132NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenaseBy similarity (EC:1.2.1.12By similarity)
Short name:
GAPDHBy similarity
Alternative name(s):
NAD-dependent glyceraldehyde-3-phosphate dehydrogenaseBy similarity
Gene namesi
Name:gap
Synonyms:hexC
Ordered Locus Names:PA3195
OrganismiPseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Taxonomic identifieri208964 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaePseudomonas
Proteomesi
  • UP000002438 Componenti: Chromosome

Organism-specific databases

PseudoCAPiPA3195.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 334334Glyceraldehyde-3-phosphate dehydrogenasePRO_0000145673Add
BLAST

Proteomic databases

PaxDbiP27726.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi208964.PA3195.

Structurei

3D structure databases

ProteinModelPortaliP27726.
SMRiP27726. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni153 – 1553Glyceraldehyde 3-phosphate bindingBy similarity
Regioni212 – 2132Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiP27726.
KOiK00134.
OMAiCSDVSTT.
PhylomeDBiP27726.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTIRLAINGF GRIGRNVLRA LYTGHYREQL QVVAINDLGD AAVNAHLFQY
60 70 80 90 100
DSVHGHFPGE VEHDAESLRV MGDRIAVSAI RNPAELPWKS LGVDIVLECT
110 120 130 140 150
GLFTSRDKAA AHLQAGAGKV LISAPGKDVE ATVVYGVNHE VLRASHRIVS
160 170 180 190 200
NASCTTNCLA PVAQVLHREL GIEHGLMTTI HAYTNDQNLS DVYHPDLYRA
210 220 230 240 250
RSATQSMIPT KTGAAEAVGL VLPELAGKLT GLAVRVPVIN VSLVDLTVQV
260 270 280 290 300
ARDTSVDEVN RLLREASEGS PVLGYNTQPL VSVDFNHDPR SSIFDANHTK
310 320 330
VSGRLVKAMA WYDNEWGFSN RMLDSALALA AARD
Length:334
Mass (Da):36,170
Last modified:December 8, 2000 - v3
Checksum:i57A5E2D257DAFF26
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti140 – 1423EVL → RGVV in AAA03488 (PubMed:8045900).Curated
Sequence conflicti268 – 2681E → K in AAA03488 (PubMed:8045900).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74256 Unassigned DNA. Translation: AAA03488.1.
AE004091 Genomic DNA. Translation: AAG06583.1.
PIRiH83246.
RefSeqiNP_251885.1. NC_002516.2.
WP_003114837.1. NZ_ASJY01000517.1.

Genome annotation databases

EnsemblBacteriaiAAG06583; AAG06583; PA3195.
GeneIDi882829.
KEGGipae:PA3195.
PATRICi19840961. VBIPseAer58763_3341.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M74256 Unassigned DNA. Translation: AAA03488.1.
AE004091 Genomic DNA. Translation: AAG06583.1.
PIRiH83246.
RefSeqiNP_251885.1. NC_002516.2.
WP_003114837.1. NZ_ASJY01000517.1.

3D structure databases

ProteinModelPortaliP27726.
SMRiP27726. Positions 2-333.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi208964.PA3195.

Proteomic databases

PaxDbiP27726.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAG06583; AAG06583; PA3195.
GeneIDi882829.
KEGGipae:PA3195.
PATRICi19840961. VBIPseAer58763_3341.

Organism-specific databases

PseudoCAPiPA3195.

Phylogenomic databases

eggNOGiENOG4105C17. Bacteria.
COG0057. LUCA.
HOGENOMiHOG000071679.
InParanoidiP27726.
KOiK00134.
OMAiCSDVSTT.
PhylomeDBiP27726.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P_PSEAE
AccessioniPrimary (citable) accession number: P27726
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: December 8, 2000
Last modified: September 7, 2016
This is version 120 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.