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Reviewed, UniProtKB/Swiss-Prot P27725 (AK1H_SERMA)

Last modified July 22, 2008. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase 1
Alternative name(s):
    Aspartokinase I/homoserine dehydrogenase I
      Short name=AKI-HDI
Including the following 2 domains:
    1- Recommended name:
            Aspartokinase
              EC=2.7.2.4
    2- Recommended name:
            Homoserine dehydrogenase
              EC=1.1.1.3
Gene names
Name: thrA
Synonyms: thrA1, thrA2
OrganismSerratia marcescens
Taxonomic identifier615 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeSerratia

Protein attributes

Sequence length819 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

General annotation (Comments)

Ontologies

Keywords

   Biological processAmino-acid biosynthesis
Threonine biosynthesis
   DomainRepeat
   LigandNADP
   Molecular functionKinase
Oxidoreductase
Transferase
   Technical termAllosteric enzyme
Multifunctional enzyme

Gene Ontology (GO)

None. [Check GOA]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical view

Molecule processing

Chain1 – 819819Bifunctional aspartokinase/homoserine dehydrogenase 1

Regions

Domain316 – 38570ACT 1
Domain397 – 46872ACT 2
Nucleotide binding471 – 4788NADP Potential
Region1 – 249249Aspartokinase By similarity
Region250 – 470221Interface By similarity
Region471 – 819349Homoserine dehydrogenase By similarity

Natural variations

Natural variant3301G → D in strain: Sr41 / 8000, Sr41 / HNr21; loss of feedback inhibition.
Natural variant3521S → F in strain: Sr41 / TLr156; loss of feedback inhibition.
Natural variant4791A → T in strain: Sr41 / HNr59; Thr-resistant HDI.

Sequences

Sequence LengthMass (Da)Tools
P27725-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 1F18552B036A8E39

FASTA81988,495
        10         20         30         40         50         60 
MRVLKFGGTS VANAERFLRV ADIMESNARQ GQVATVLSAP AKITNHLVAM IDKTVAGQDI 

        70         80         90        100        110        120 
LPNMSDAERI FADLLSGLAQ ALPGFEYDRL KGVVDQEFAQ LKQVLHGVSL LGQCPDSVNA 

       130        140        150        160        170        180 
AIICRGEKLS IAIMEGVFRA KGYPVTVINP VEKLLAQGHY LESTVDIAES TLRIAAAAIP 

       190        200        210        220        230        240 
ADHIVLMAGF TAGNDKGELV VLGRNGSDYS AAVLAACLRA DCCEIWTDVD GVYTCDPRTV 

       250        260        270        280        290        300 
PDARLLKSMS YQEAMELSYF GAKVLHPRTI TPIAQFQIPC LIKNTSNPQA PGTLIGKDST 

       310        320        330        340        350        360 
DADMPVKGIT NLNNMAMINV SGPGMKGMVG MAARVFAVMS RAGISVVLIT QSSSEYSISF 

       370        380        390        400        410        420 
CVPQGELQRA RRALEEEFYL ELKDGVLDPL DVMERLAIIS VVGDGMRTLR GISARFFSAL 

       430        440        450        460        470        480 
ARANINIVAI AQGSSERSIS VVVSNDSATT GVRVSHQMLF NTDQVIEVFV IGVGGVGGAL 

       490        500        510        520        530        540 
IEQIYRQQPW LKQKHIDLRV CGIANSRVML TNVHGIALDS WRDALAGAQE PFNLGRLIRL 

       550        560        570        580        590        600 
VKEYHLLNPV IVDCTSSQAV ADQYVDFLAD GFHVVTPNKK ANTSSMNYYQ QLRAAAAGSH 

       610        620        630        640        650        660 
RKFLYDTNVG AGLPVIENLQ NLLNAGDELV RFSGILSGSL SFIFGKLDEG LSLSAATLQA 

       670        680        690        700        710        720 
RANGYTEPDP RDDLSGMDVA RKLLILAREA GYKLELSDIE VEPVLPPSFD ASGDVDTFLA 

       730        740        750        760        770        780 
RLPELDKEFA RNVANAAEQG KVLRYVGLID EGRCKVRIEA VDGNDPLYKV KNGENALAFY 

       790        800        810 
SRYYQPLPLV LRGYGAGNDV TAAGVFADLL RTLSWKLGV 

« Hide

References

[1]"Nucleotide sequence of the Serratia marcescens threonine operon and analysis of the threonine operon mutations which alter feedback inhibition of both aspartokinase I and homoserine dehydrogenase I."
Omori K., Suzuki S., Komatsubara S.
J. Bacteriol. 175:785-794(1993) [PubMed: 8423151] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Sr41 / 8000, Sr41 / HNr21, Sr41 / HNr59 and Sr41 / TLr156.

Cross-references

Sequence databases

D10385 Genomic DNA. Translation: BAA38474.1.
D10386 Genomic DNA. Translation: BAA38477.1.
D10387 Genomic DNA. Translation: BAA38480.1.
X60821 Genomic DNA. Translation: CAA43212.1.
PIRB47057.

3D structure databases

HSSPHSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBaseSearch...

Family and domain databases

InterProIPR002912. ACT_bd.
IPR001048. Asp/Glu/Uridylate_kinase.
IPR005106. Asp/hSer_DHase_NAD-bd.
IPR001341. Asp_kin_reg.
IPR011147. bifunc_aspartokin/hSer_DHase.
IPR001342. hSer_DHase_cat.
IPR016040. NAD(P)-bd.
[Graphical view]
Gene3DG3DSA:3.40.1160.10. Aa_kinase. 1 hit.
G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PfamPF00696. AA_kinase. 1 hit.
PF01842. ACT. 2 hits.
PF00742. Homoserine_dh. 1 hit.
PF03447. NAD_binding_3. 1 hit.
[Graphical view]
PIRSFPIRSF000727. ThrA. 1 hit.
TIGRFAMsTIGR00657. asp_kinases. 1 hit.
PROSITEPS00324. ASPARTOKINASE. 1 hit.
PS01042. HOMOSER_DHGENASE. 1 hit.
[Graphical view]
ProDomP27725.
[Graphical view] [Entries sharing at least one domain]
BLOCKSSearch...

Other Resources

ProtoNetSearch...

Entry information

Entry nameAK1H_SERMA
AccessionPrimary (citable) accession number: P27725
Secondary accession number(s): Q59936 expand/collapse secondary AC list , Q59937, Q60127, Q60157
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 22, 2008
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

UniProtKB secondary accession numbers

Index of UniProtKB secondary accession numbers

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents