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Protein

Phorbol ester/diacylglycerol-binding protein unc-13

Gene

unc-13

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May form part of a signal transduction pathway, transducing the signal from diacylglycerol to effector functions. One such function could be the release of neurotransmitter from neurons.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri693 – 74250Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1034 – 108451Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • intracellular signal transduction Source: InterPro
  • regulation of oviposition Source: WormBase
  • regulation of pharyngeal pumping Source: WormBase
  • synaptic vesicle exocytosis Source: WormBase
Complete GO annotation...

Keywords - Ligandi

Calmodulin-binding, Metal-binding, Zinc

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Phorbol ester/diacylglycerol-binding protein unc-13
Alternative name(s):
Uncoordinated protein 13
Gene namesi
Name:unc-13
ORF Names:ZK524.2
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiZK524.2a; CE15371; WBGene00006752; unc-13.
ZK524.2c; CE34624; WBGene00006752; unc-13.
ZK524.2d; CE34625; WBGene00006752; unc-13.
ZK524.2e; CE34626; WBGene00006752; unc-13.

Subcellular locationi

GO - Cellular componenti

  • intracellular Source: GOC
  • presynaptic active zone Source: WormBase
  • synapse Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Worms exhibit diverse nervous system defects.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 21552155Phorbol ester/diacylglycerol-binding protein unc-13PRO_0000188583Add
BLAST

Proteomic databases

EPDiP27715.
PaxDbiP27715.
PRIDEiP27715.

Expressioni

Gene expression databases

ExpressionAtlasiP27715. baseline and differential.

Interactioni

Subunit structurei

Interacts with cmd-1 in the presence of Ca2+.1 Publication

Protein-protein interaction databases

STRINGi6239.ZK524.2d.

Structurei

3D structure databases

ProteinModelPortaliP27715.
SMRiP27715. Positions 1035-1084, 1155-1287.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 9676C2 1PROSITE-ProRule annotationAdd
BLAST
Domaini802 – 908107C2 2PROSITE-ProRule annotationAdd
BLAST
Domaini1552 – 1695144MHD1PROSITE-ProRule annotationAdd
BLAST
Domaini1798 – 1954157MHD2PROSITE-ProRule annotationAdd
BLAST
Domaini1972 – 2077106C2 3PROSITE-ProRule annotationAdd
BLAST

Domaini

The phorbol ester binding activity is zinc and calcium-dependent.

Sequence similaritiesi

Belongs to the unc-13 family.Curated
Contains 3 C2 domains.PROSITE-ProRule annotation
Contains 1 MHD1 (MUNC13 homology domain 1) domain.PROSITE-ProRule annotation
Contains 1 MHD2 (MUNC13 homology domain 2) domain.PROSITE-ProRule annotation
Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri693 – 74250Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri1034 – 108451Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
GeneTreeiENSGT00730000110590.
HOGENOMiHOG000231404.
InParanoidiP27715.
OMAiESNIFFP.
OrthoDBiEOG76738V.
PhylomeDBiP27715.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR020454. DAG/PE-bd.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 3 hits.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 6 (identifier: P27715-6) [UniParc]FASTAAdd to basket

Also known as: d

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDDVGDYNDD QLSDGMSMRL LCITIKKARL QGAVDEFNSY VTVKLQTVKS
60 70 80 90 100
TTVAVRGNLP CWEQEFIFET NRPDDGMVLE LWAKGVLWDK LIGVHYMPLS
110 120 130 140 150
EIRYSNAAGS GQWLQMDHEL ETRNGQTVGT RGPTGHNLLT DVRFELPFDV
160 170 180 190 200
QGHDEDIQSR LLALNGLIEH DQLGPNNHHR APFNHSGLSE DSDYTSDVSV
210 220 230 240 250
PVNHHQLHPN SSAHQYESHL HPHRTRQLLH TREGAASYED EEDAYHARHQ
260 270 280 290 300
PESDDYNHQD TYDQHSSYYN DEYAPSGSSS QYQRQGYQDQ DQQHQNIYED
310 320 330 340 350
TVTPVREFGE SAVPPAASSS RRQFDQVYGY ASSSEERYDT PMSSGRLPRD
360 370 380 390 400
EPILEHSEPE YVYDQNGYPE EDNYGINPTY SEDHFEGQTN DYSTTHQEPN
410 420 430 440 450
DFRNDYNSSY QREYWNESEP LSYNSRPPNG HIRTGANTWR EPSTSSRPTS
460 470 480 490 500
SQAWNYQDDT HQYDEVDRGS RVSFTRTPSV DRTDRPSESG GGFYDEMSES
510 520 530 540 550
GRPGRPDSHH NWRYDSIQEE DNEKDNWKQH VEGYEEGQEE KQKDNQKPND
560 570 580 590 600
HSAASPQDHY HRSDSTAQQD FGNNIVRQTI QEEEEKRNYQ ELWHNAYKRV
610 620 630 640 650
CADLGIKSSQ ASLVATTGGT LLQNLFLYRP KLAPAGHGAT ASQFQSPAGA
660 670 680 690 700
TRFYANHNNN NNNVSKNELD STTETPPDPS RTPSTSSMNP VPSLAVPMSP
710 720 730 740 750
GPYLNSDPPS PVSPNPQIKR SIYRIKESYE DRNGGRERIY TTNLVSVYLE
760 770 780 790 800
KMKPPDELEE GSSGSMRETQ NEIKNGTQLH NAESNIFFPQ DSVPKSISYN
810 820 830 840 850
AGNLKNTSIT TSKTSSAITN HSSLPPQPPS KPASRDSDPM KQLLTFSKSF
860 870 880 890 900
KKVRRVRSAM PRRRKRKRVK IKKSRSCPIL WKTEKTPHPM KSKSMTCIRI
910 920 930 940 950
PKKTVIAPLR KEIKIVRMKP PAARCESDSK AHKKKNLLDV YKDMGKSTVL
960 970 980 990 1000
DGNGSSAANA FYKSIDAAPN MNVARTKTSI PLVSELVLKT MATKRAQAGL
1010 1020 1030 1040 1050
ANAARTTFSD TELKTHVYKK TLQALIYPIS ATTPHNFATT TFQTPTFCYE
1060 1070 1080 1090 1100
CEGLLWGLAR QGLRCTQCQV KVHDKCRELL SADCLQRAAE KSTKHGEADR
1110 1120 1130 1140 1150
TQSLVNVIRD RMKIQEQNKP EVFQMIRTVF DVDENIQKET LKTVKASILE
1160 1170 1180 1190 1200
GSSKWSAKIT LTVLCAQGLI AKDKTGKSDP YVTAQVGKTK RRTRTIHQEL
1210 1220 1230 1240 1250
NPVWNEKFHF ECHNSTDRIK VRVWDEDNDL KSKLRQKLTR ESDDFLGQTV
1260 1270 1280 1290 1300
IEVRTLSGEM DVWYNLEKRT DKSAVSGAIR LHINVEIKGE EKLAPYHVQY
1310 1320 1330 1340 1350
TCLHEHLFAA HCVDEEVKLP KVRGEDSWKV CFQETGQEIA EEFAMRYGIE
1360 1370 1380 1390 1400
SIYQAMTHFA CLCTRYMCAG VPAVLSTLLA NINAYYAHTT ATSAVSAPDR
1410 1420 1430 1440 1450
FAASNFGKER FVKLLDQLHN SLRIDLSAYR NHFPSSSPAK LQDLKSTVDL
1460 1470 1480 1490 1500
LTSITFFRMK VLELASPPRA STVVRECAKA CMQQTYQLMF ESCAEQFPIL
1510 1520 1530 1540 1550
DTSVQFWYEF IDYIMRVIEE DQKNYTPALN QFPQELNVGN LSAETLWSMY
1560 1570 1580 1590 1600
KNDLKMALEE HAQKKRCKTP EYMNLYFKVK GFYFKYVADL STYKSSIPEF
1610 1620 1630 1640 1650
PAWFIPFVMD WLNENDEHSM DILRNAYNVD KADNFPQTSE HTKFSNSVVD
1660 1670 1680 1690 1700
VFTQLNAALK LLKQMDCPNP EVAADMMKRF SKTLNKVLLA YADMVQKDFP
1710 1720 1730 1740 1750
KFAHDEKLAC ILMNNVQQLR VQLEKIYETM GGAELDEHIG QVLTVLQKKL
1760 1770 1780 1790 1800
NSVLDRLSAE FVTTLEPHIH EQTIKLGMLL VKIKGPQLQK TQVQPEADAV
1810 1820 1830 1840 1850
LEPLMDLLEG SLRRYADQCE KTVLKYILKE LWKITIVNME KRVVLPPLSD
1860 1870 1880 1890 1900
KALLKQLPNA KIGDVTKLMS TNIQSIKGMN SVKDMMDMAR ESEKSLTPRQ
1910 1920 1930 1940 1950
CTVLDCALDA IKDSFHASGK GLKKSFFEKS PELQSLKYAL SLYTQTTEQL
1960 1970 1980 1990 2000
IKTFITSQRQ QDLPSQEQPV GEVSVQVDLF SHPGTGEQKV TVKILAANDL
2010 2020 2030 2040 2050
RWQTSSAFKP FVEVHLVGPH LSDKKRKWST KTKAGNWAPK FNETFHFFLG
2060 2070 2080 2090 2100
NEGEPEHYEL MFQVKDYCFA RDDRVVGVGV LQLSSVVDQA GSCAMWVQLG
2110 2120 2130 2140 2150
TRLHIDETGL ILLRILSQRQ TDEVAKDFVR LKTECRYETE TVMAASASSQ

NINRT
Length:2,155
Mass (Da):245,231
Last modified:March 29, 2004 - v4
Checksum:iB660C5926F1D26A2
GO
Isoform 1 (identifier: P27715-1) [UniParc]FASTAAdd to basket

Also known as: a

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.
     987-989: Missing.

Note: No experimental confirmation available.
Show »
Length:1,813
Mass (Da):207,251
Checksum:i649D1B39F18ACFD2
GO
Isoform 2 (identifier: P27715-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.
     1629-1647: VDKADNFPQTSEHTKFSNS → CRQADTSHKHQNTQILEH

Show »
Length:1,815
Mass (Da):207,586
Checksum:i7985FE38FE754336
GO
Isoform 3 (identifier: P27715-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: No experimental confirmation available.
Show »
Length:2,140
Mass (Da):243,591
Checksum:iEB86DDE71B5D98AF
GO
Isoform 5 (identifier: P27715-5) [UniParc]FASTAAdd to basket

Also known as: c

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     609-947: Missing.

Note: No experimental confirmation available.
Show »
Length:1,475
Mass (Da):168,697
Checksum:i654E35E22754B310
GO
Isoform 7 (identifier: P27715-7) [UniParc]FASTAAdd to basket

Also known as: e

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.

Note: No experimental confirmation available.
Show »
Length:1,816
Mass (Da):207,591
Checksum:i65DEC63A1157E67D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti157 – 1571I → V in AAA93094 (PubMed:2062851).Curated
Sequence conflicti1873 – 18731I → N in AAA93094 (PubMed:2062851).Curated
Sequence conflicti1990 – 19901V → A in AAA93094 (PubMed:2062851).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 341341Missing in isoform 5. CuratedVSP_009724Add
BLAST
Alternative sequencei1 – 1515Missing in isoform 3. CuratedVSP_004485Add
BLAST
Alternative sequencei609 – 947339Missing in isoform 1, isoform 2, isoform 5 and isoform 7. 1 PublicationVSP_009726Add
BLAST
Alternative sequencei987 – 9893Missing in isoform 1. CuratedVSP_009727
Alternative sequencei1629 – 164719VDKAD…KFSNS → CRQADTSHKHQNTQILEH in isoform 2. 1 PublicationVSP_009728Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62830 mRNA. Translation: AAA93094.1.
U50735 Genomic DNA. Translation: AAA99780.1.
U50735 Genomic DNA. Translation: AAA99781.1.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAA98147.1.
Z73912, Z92779 Genomic DNA. Translation: CAD56619.2.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAD90190.2.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAE11317.1.
PIRiA41101.
T19295.
T27886.
RefSeqiNP_001021871.1. NM_001026700.1. [P27715-1]
NP_001021872.1. NM_001026701.1. [P27715-5]
NP_001021873.1. NM_001026702.1. [P27715-6]
NP_001021874.1. NM_001026703.2. [P27715-7]
UniGeneiCel.16847.

Genome annotation databases

EnsemblMetazoaiZK524.2d; ZK524.2d; WBGene00006752. [P27715-6]
GeneIDi172497.
UCSCiZK524.2e. c. elegans. [P27715-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62830 mRNA. Translation: AAA93094.1.
U50735 Genomic DNA. Translation: AAA99780.1.
U50735 Genomic DNA. Translation: AAA99781.1.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAA98147.1.
Z73912, Z92779 Genomic DNA. Translation: CAD56619.2.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAD90190.2.
Z73912, Z79694, Z92779 Genomic DNA. Translation: CAE11317.1.
PIRiA41101.
T19295.
T27886.
RefSeqiNP_001021871.1. NM_001026700.1. [P27715-1]
NP_001021872.1. NM_001026701.1. [P27715-5]
NP_001021873.1. NM_001026702.1. [P27715-6]
NP_001021874.1. NM_001026703.2. [P27715-7]
UniGeneiCel.16847.

3D structure databases

ProteinModelPortaliP27715.
SMRiP27715. Positions 1035-1084, 1155-1287.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6239.ZK524.2d.

Protein family/group databases

TCDBi1.F.1.1.3. the synaptosomal vesicle fusion pore (svf-pore) family.

Proteomic databases

EPDiP27715.
PaxDbiP27715.
PRIDEiP27715.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiZK524.2d; ZK524.2d; WBGene00006752. [P27715-6]
GeneIDi172497.
UCSCiZK524.2e. c. elegans. [P27715-1]

Organism-specific databases

CTDi43841.
WormBaseiZK524.2a; CE15371; WBGene00006752; unc-13.
ZK524.2c; CE34624; WBGene00006752; unc-13.
ZK524.2d; CE34625; WBGene00006752; unc-13.
ZK524.2e; CE34626; WBGene00006752; unc-13.

Phylogenomic databases

eggNOGiKOG1011. Eukaryota.
ENOG410XS5D. LUCA.
GeneTreeiENSGT00730000110590.
HOGENOMiHOG000231404.
InParanoidiP27715.
OMAiESNIFFP.
OrthoDBiEOG76738V.
PhylomeDBiP27715.

Miscellaneous databases

NextBioi875765.
PROiP27715.

Gene expression databases

ExpressionAtlasiP27715. baseline and differential.

Family and domain databases

Gene3Di2.60.40.150. 3 hits.
InterProiIPR000008. C2_dom.
IPR010439. CAPS_dom.
IPR020454. DAG/PE-bd.
IPR014770. Munc13_1.
IPR014772. Munc13_dom-2.
IPR019558. Munc13_subgr_dom-2.
IPR002219. PE/DAG-bd.
IPR027080. Unc-13.
[Graphical view]
PANTHERiPTHR10480. PTHR10480. 3 hits.
PfamiPF00130. C1_1. 1 hit.
PF00168. C2. 3 hits.
PF06292. DUF1041. 1 hit.
PF10540. Membr_traf_MHD. 1 hit.
[Graphical view]
PRINTSiPR00360. C2DOMAIN.
PR00008. DAGPEDOMAIN.
SMARTiSM00109. C1. 1 hit.
SM00239. C2. 3 hits.
SM01145. DUF1041. 1 hit.
[Graphical view]
SUPFAMiSSF49562. SSF49562. 3 hits.
PROSITEiPS50004. C2. 2 hits.
PS51258. MHD1. 1 hit.
PS51259. MHD2. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans."
    Maruyama I.N., Brenner S.
    Proc. Natl. Acad. Sci. U.S.A. 88:5729-5733(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DISRUPTION PHENOTYPE.
    Strain: Bristol N2.
  2. "Expression of multiple UNC-13 proteins in the Caenorhabditis elegans nervous system."
    Kohn R.E., Duerr J.S., McManus J.R., Duke A., Rakow T.L., Maruyama H., Moulder G., Maruyama I.N., Barstead R.J., Rand J.B.
    Mol. Biol. Cell 11:3441-3452(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1/2/6/7 AND 3).
    Strain: Bristol N2.
  3. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  4. "The Caenorhabditis elegans unc-13 gene product is a phospholipid-dependent high-affinity phorbol ester receptor."
    Ahmed S., Maruyama I.N., Kozma R., Lee J., Brenner S., Lim L.
    Biochem. J. 287:995-999(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: ZINC AND PHORBOL-ESTERS BINDING.
  5. "Ca(2+)/Calmodulin-binding proteins from the C. elegans proteome."
    Shen X., Valencia C.A., Gao W., Cotten S.W., Dong B., Huang B.C., Liu R.
    Cell Calcium 43:444-456(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CMD-1.

Entry informationi

Entry nameiUNC13_CAEEL
AccessioniPrimary (citable) accession number: P27715
Secondary accession number(s): O17665
, Q23512, Q7YSR2, Q867Z2, Q8I095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: March 29, 2004
Last modified: May 11, 2016
This is version 141 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.