P27715 (UNC13_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phorbol ester/diacylglycerol-binding protein unc-13

Alternative name(s):
Uncoordinated protein 13

Gene names

Name:	unc-13
ORF Names:	ZK524.2

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	2155 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May form part of a signal transduction pathway, transducing the signal from diacylglycerol to effector functions. One such function could be the release of neurotransmitter from neurons.
Domain	The phorbol ester binding activity is zinc and calcium-dependent.
Disruption phenotype	Worms exhibit diverse nervous system defects. Ref.1
Sequence similarities	Belongs to the unc-13 family. Contains 3 C2 domains. Contains 1 MHD1 (MUNC13 homology domain 1) domain. Contains 1 MHD2 (MUNC13 homology domain 2) domain. Contains 1 phorbol-ester/DAG-type zinc finger.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro regulation of oviposition Inferred from mutant phenotype PubMed 14704167. Source: WormBase regulation of pharyngeal pumping Inferred from mutant phenotype PubMed 14704167. Source: WormBase synaptic transmission Inferred from electronic annotation. Source: InterPro
Cellular_component	presynaptic active zone Inferred from direct assay PubMed 15976086. Source: WormBase
Molecular_function	diacylglycerol binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 6 isoforms produced by alternative splicing. [Align] [Select]

Isoform 6 (identifier: P27715-6)

Also known as: d;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 1 (identifier: P27715-1)

Also known as: a;

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.
     987-989: Missing.

Note: No experimental confirmation available.

Isoform 2 (identifier: P27715-2)

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.
     1629-1647: VDKADNFPQTSEHTKFSNS → CRQADTSHKHQNTQILEH

Isoform 3 (identifier: P27715-3)

The sequence of this isoform differs from the canonical sequence as follows:
     1-15: Missing.

Note: No experimental confirmation available.

Isoform 5 (identifier: P27715-5)

Also known as: c;

The sequence of this isoform differs from the canonical sequence as follows:
     1-341: Missing.
     609-947: Missing.

Note: No experimental confirmation available.

Isoform 7 (identifier: P27715-7)

Also known as: e;

The sequence of this isoform differs from the canonical sequence as follows:
     609-947: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2155	2155	Phorbol ester/diacylglycerol-binding protein unc-13		PRO_0000188583
Regions
	Domain	21 – 96	76	C2 1
	Domain	802 – 908	107	C2 2
	Domain	1552 – 1695	144	MHD1
	Domain	1798 – 1954	157	MHD2
	Domain	1972 – 2077	106	C2 3
	Zinc finger	693 – 742	50	Phorbol-ester/DAG-type
	Zinc finger	1034 – 1084	51	Phorbol-ester/DAG-type
Natural variations
	Alternative sequence	1 – 341	341	Missing in isoform 5.		VSP_009724
	Alternative sequence	1 – 15	15	Missing in isoform 3.		VSP_004485
	Alternative sequence	609 – 947	339	Missing in isoform 1, isoform 2, isoform 5 and isoform 7.		VSP_009726
	Alternative sequence	987 – 989	3	Missing in isoform 1.		VSP_009727
	Alternative sequence	1629 – 1647	19	VDKAD…KFSNS → CRQADTSHKHQNTQILEH in isoform 2.		VSP_009728
Experimental info
	Sequence conflict	157	1	I → V in AAA93094. Ref.1
	Sequence conflict	1873	1	I → N in AAA93094. Ref.1
	Sequence conflict	1990	1	V → A in AAA93094. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 6 (d) [UniParc]. Last modified March 29, 2004. Version 4. Checksum: B660C5926F1D26A2	FASTA	2,155	245,231
	10 20 30 40 50 60 MDDVGDYNDD QLSDGMSMRL LCITIKKARL QGAVDEFNSY VTVKLQTVKS TTVAVRGNLP 70 80 90 100 110 120 CWEQEFIFET NRPDDGMVLE LWAKGVLWDK LIGVHYMPLS EIRYSNAAGS GQWLQMDHEL 130 140 150 160 170 180 ETRNGQTVGT RGPTGHNLLT DVRFELPFDV QGHDEDIQSR LLALNGLIEH DQLGPNNHHR 190 200 210 220 230 240 APFNHSGLSE DSDYTSDVSV PVNHHQLHPN SSAHQYESHL HPHRTRQLLH TREGAASYED 250 260 270 280 290 300 EEDAYHARHQ PESDDYNHQD TYDQHSSYYN DEYAPSGSSS QYQRQGYQDQ DQQHQNIYED 310 320 330 340 350 360 TVTPVREFGE SAVPPAASSS RRQFDQVYGY ASSSEERYDT PMSSGRLPRD EPILEHSEPE 370 380 390 400 410 420 YVYDQNGYPE EDNYGINPTY SEDHFEGQTN DYSTTHQEPN DFRNDYNSSY QREYWNESEP 430 440 450 460 470 480 LSYNSRPPNG HIRTGANTWR EPSTSSRPTS SQAWNYQDDT HQYDEVDRGS RVSFTRTPSV 490 500 510 520 530 540 DRTDRPSESG GGFYDEMSES GRPGRPDSHH NWRYDSIQEE DNEKDNWKQH VEGYEEGQEE 550 560 570 580 590 600 KQKDNQKPND HSAASPQDHY HRSDSTAQQD FGNNIVRQTI QEEEEKRNYQ ELWHNAYKRV 610 620 630 640 650 660 CADLGIKSSQ ASLVATTGGT LLQNLFLYRP KLAPAGHGAT ASQFQSPAGA TRFYANHNNN 670 680 690 700 710 720 NNNVSKNELD STTETPPDPS RTPSTSSMNP VPSLAVPMSP GPYLNSDPPS PVSPNPQIKR 730 740 750 760 770 780 SIYRIKESYE DRNGGRERIY TTNLVSVYLE KMKPPDELEE GSSGSMRETQ NEIKNGTQLH 790 800 810 820 830 840 NAESNIFFPQ DSVPKSISYN AGNLKNTSIT TSKTSSAITN HSSLPPQPPS KPASRDSDPM 850 860 870 880 890 900 KQLLTFSKSF KKVRRVRSAM PRRRKRKRVK IKKSRSCPIL WKTEKTPHPM KSKSMTCIRI 910 920 930 940 950 960 PKKTVIAPLR KEIKIVRMKP PAARCESDSK AHKKKNLLDV YKDMGKSTVL DGNGSSAANA 970 980 990 1000 1010 1020 FYKSIDAAPN MNVARTKTSI PLVSELVLKT MATKRAQAGL ANAARTTFSD TELKTHVYKK 1030 1040 1050 1060 1070 1080 TLQALIYPIS ATTPHNFATT TFQTPTFCYE CEGLLWGLAR QGLRCTQCQV KVHDKCRELL 1090 1100 1110 1120 1130 1140 SADCLQRAAE KSTKHGEADR TQSLVNVIRD RMKIQEQNKP EVFQMIRTVF DVDENIQKET 1150 1160 1170 1180 1190 1200 LKTVKASILE GSSKWSAKIT LTVLCAQGLI AKDKTGKSDP YVTAQVGKTK RRTRTIHQEL 1210 1220 1230 1240 1250 1260 NPVWNEKFHF ECHNSTDRIK VRVWDEDNDL KSKLRQKLTR ESDDFLGQTV IEVRTLSGEM 1270 1280 1290 1300 1310 1320 DVWYNLEKRT DKSAVSGAIR LHINVEIKGE EKLAPYHVQY TCLHEHLFAA HCVDEEVKLP 1330 1340 1350 1360 1370 1380 KVRGEDSWKV CFQETGQEIA EEFAMRYGIE SIYQAMTHFA CLCTRYMCAG VPAVLSTLLA 1390 1400 1410 1420 1430 1440 NINAYYAHTT ATSAVSAPDR FAASNFGKER FVKLLDQLHN SLRIDLSAYR NHFPSSSPAK 1450 1460 1470 1480 1490 1500 LQDLKSTVDL LTSITFFRMK VLELASPPRA STVVRECAKA CMQQTYQLMF ESCAEQFPIL 1510 1520 1530 1540 1550 1560 DTSVQFWYEF IDYIMRVIEE DQKNYTPALN QFPQELNVGN LSAETLWSMY KNDLKMALEE 1570 1580 1590 1600 1610 1620 HAQKKRCKTP EYMNLYFKVK GFYFKYVADL STYKSSIPEF PAWFIPFVMD WLNENDEHSM 1630 1640 1650 1660 1670 1680 DILRNAYNVD KADNFPQTSE HTKFSNSVVD VFTQLNAALK LLKQMDCPNP EVAADMMKRF 1690 1700 1710 1720 1730 1740 SKTLNKVLLA YADMVQKDFP KFAHDEKLAC ILMNNVQQLR VQLEKIYETM GGAELDEHIG 1750 1760 1770 1780 1790 1800 QVLTVLQKKL NSVLDRLSAE FVTTLEPHIH EQTIKLGMLL VKIKGPQLQK TQVQPEADAV 1810 1820 1830 1840 1850 1860 LEPLMDLLEG SLRRYADQCE KTVLKYILKE LWKITIVNME KRVVLPPLSD KALLKQLPNA 1870 1880 1890 1900 1910 1920 KIGDVTKLMS TNIQSIKGMN SVKDMMDMAR ESEKSLTPRQ CTVLDCALDA IKDSFHASGK 1930 1940 1950 1960 1970 1980 GLKKSFFEKS PELQSLKYAL SLYTQTTEQL IKTFITSQRQ QDLPSQEQPV GEVSVQVDLF 1990 2000 2010 2020 2030 2040 SHPGTGEQKV TVKILAANDL RWQTSSAFKP FVEVHLVGPH LSDKKRKWST KTKAGNWAPK 2050 2060 2070 2080 2090 2100 FNETFHFFLG NEGEPEHYEL MFQVKDYCFA RDDRVVGVGV LQLSSVVDQA GSCAMWVQLG 2110 2120 2130 2140 2150 TRLHIDETGL ILLRILSQRQ TDEVAKDFVR LKTECRYETE TVMAASASSQ NINRT
	Isoform 1 (a) [UniParc]. Checksum: 649D1B39F18ACFD2	FASTA	1,813	207,251
	10 20 30 40 50 60 MDDVGDYNDD QLSDGMSMRL LCITIKKARL QGAVDEFNSY VTVKLQTVKS TTVAVRGNLP 70 80 90 100 110 120 CWEQEFIFET NRPDDGMVLE LWAKGVLWDK LIGVHYMPLS EIRYSNAAGS GQWLQMDHEL 130 140 150 160 170 180 ETRNGQTVGT RGPTGHNLLT DVRFELPFDV QGHDEDIQSR LLALNGLIEH DQLGPNNHHR 190 200 210 220 230 240 APFNHSGLSE DSDYTSDVSV PVNHHQLHPN SSAHQYESHL HPHRTRQLLH TREGAASYED 250 260 270 280 290 300 EEDAYHARHQ PESDDYNHQD TYDQHSSYYN DEYAPSGSSS QYQRQGYQDQ DQQHQNIYED 310 320 330 340 350 360 TVTPVREFGE SAVPPAASSS RRQFDQVYGY ASSSEERYDT PMSSGRLPRD EPILEHSEPE 370 380 390 400 410 420 YVYDQNGYPE EDNYGINPTY SEDHFEGQTN DYSTTHQEPN DFRNDYNSSY QREYWNESEP 430 440 450 460 470 480 LSYNSRPPNG HIRTGANTWR EPSTSSRPTS SQAWNYQDDT HQYDEVDRGS RVSFTRTPSV 490 500 510 520 530 540 DRTDRPSESG GGFYDEMSES GRPGRPDSHH NWRYDSIQEE DNEKDNWKQH VEGYEEGQEE 550 560 570 580 590 600 KQKDNQKPND HSAASPQDHY HRSDSTAQQD FGNNIVRQTI QEEEEKRNYQ ELWHNAYKRV 610 620 630 640 650 660 CADLGIKSTV LDGNGSSAAN AFYKSIDAAP NMNVARTKTS IPLVSELTMA TKRAQAGLAN 670 680 690 700 710 720 AARTTFSDTE LKTHVYKKTL QALIYPISAT TPHNFATTTF QTPTFCYECE GLLWGLARQG 730 740 750 760 770 780 LRCTQCQVKV HDKCRELLSA DCLQRAAEKS TKHGEADRTQ SLVNVIRDRM KIQEQNKPEV 790 800 810 820 830 840 FQMIRTVFDV DENIQKETLK TVKASILEGS SKWSAKITLT VLCAQGLIAK DKTGKSDPYV 850 860 870 880 890 900 TAQVGKTKRR TRTIHQELNP VWNEKFHFEC HNSTDRIKVR VWDEDNDLKS KLRQKLTRES 910 920 930 940 950 960 DDFLGQTVIE VRTLSGEMDV WYNLEKRTDK SAVSGAIRLH INVEIKGEEK LAPYHVQYTC 970 980 990 1000 1010 1020 LHEHLFAAHC VDEEVKLPKV RGEDSWKVCF QETGQEIAEE FAMRYGIESI YQAMTHFACL 1030 1040 1050 1060 1070 1080 CTRYMCAGVP AVLSTLLANI NAYYAHTTAT SAVSAPDRFA ASNFGKERFV KLLDQLHNSL 1090 1100 1110 1120 1130 1140 RIDLSAYRNH FPSSSPAKLQ DLKSTVDLLT SITFFRMKVL ELASPPRAST VVRECAKACM 1150 1160 1170 1180 1190 1200 QQTYQLMFES CAEQFPILDT SVQFWYEFID YIMRVIEEDQ KNYTPALNQF PQELNVGNLS 1210 1220 1230 1240 1250 1260 AETLWSMYKN DLKMALEEHA QKKRCKTPEY MNLYFKVKGF YFKYVADLST YKSSIPEFPA 1270 1280 1290 1300 1310 1320 WFIPFVMDWL NENDEHSMDI LRNAYNVDKA DNFPQTSEHT KFSNSVVDVF TQLNAALKLL 1330 1340 1350 1360 1370 1380 KQMDCPNPEV AADMMKRFSK TLNKVLLAYA DMVQKDFPKF AHDEKLACIL MNNVQQLRVQ 1390 1400 1410 1420 1430 1440 LEKIYETMGG AELDEHIGQV LTVLQKKLNS VLDRLSAEFV TTLEPHIHEQ TIKLGMLLVK 1450 1460 1470 1480 1490 1500 IKGPQLQKTQ VQPEADAVLE PLMDLLEGSL RRYADQCEKT VLKYILKELW KITIVNMEKR 1510 1520 1530 1540 1550 1560 VVLPPLSDKA LLKQLPNAKI GDVTKLMSTN IQSIKGMNSV KDMMDMARES EKSLTPRQCT 1570 1580 1590 1600 1610 1620 VLDCALDAIK DSFHASGKGL KKSFFEKSPE LQSLKYALSL YTQTTEQLIK TFITSQRQQD 1630 1640 1650 1660 1670 1680 LPSQEQPVGE VSVQVDLFSH PGTGEQKVTV KILAANDLRW QTSSAFKPFV EVHLVGPHLS 1690 1700 1710 1720 1730 1740 DKKRKWSTKT KAGNWAPKFN ETFHFFLGNE GEPEHYELMF QVKDYCFARD DRVVGVGVLQ 1750 1760 1770 1780 1790 1800 LSSVVDQAGS CAMWVQLGTR LHIDETGLIL LRILSQRQTD EVAKDFVRLK TECRYETETV 1810 MAASASSQNI NRT
	Isoform 2 [UniParc]. Checksum: 7985FE38FE754336	FASTA	1,815	207,586
	10 20 30 40 50 60 MDDVGDYNDD QLSDGMSMRL LCITIKKARL QGAVDEFNSY VTVKLQTVKS TTVAVRGNLP 70 80 90 100 110 120 CWEQEFIFET NRPDDGMVLE LWAKGVLWDK LIGVHYMPLS EIRYSNAAGS GQWLQMDHEL 130 140 150 160 170 180 ETRNGQTVGT RGPTGHNLLT DVRFELPFDV QGHDEDIQSR LLALNGLIEH DQLGPNNHHR 190 200 210 220 230 240 APFNHSGLSE DSDYTSDVSV PVNHHQLHPN SSAHQYESHL HPHRTRQLLH TREGAASYED 250 260 270 280 290 300 EEDAYHARHQ PESDDYNHQD TYDQHSSYYN DEYAPSGSSS QYQRQGYQDQ DQQHQNIYED 310 320 330 340 350 360 TVTPVREFGE SAVPPAASSS RRQFDQVYGY ASSSEERYDT PMSSGRLPRD EPILEHSEPE 370 380 390 400 410 420 YVYDQNGYPE EDNYGINPTY SEDHFEGQTN DYSTTHQEPN DFRNDYNSSY QREYWNESEP 430 440 450 460 470 480 LSYNSRPPNG HIRTGANTWR EPSTSSRPTS SQAWNYQDDT HQYDEVDRGS RVSFTRTPSV 490 500 510 520 530 540 DRTDRPSESG GGFYDEMSES GRPGRPDSHH NWRYDSIQEE DNEKDNWKQH VEGYEEGQEE 550 560 570 580 590 600 KQKDNQKPND HSAASPQDHY HRSDSTAQQD FGNNIVRQTI QEEEEKRNYQ ELWHNAYKRV 610 620 630 640 650 660 CADLGIKSTV LDGNGSSAAN AFYKSIDAAP NMNVARTKTS IPLVSELVLK TMATKRAQAG 670 680 690 700 710 720 LANAARTTFS DTELKTHVYK KTLQALIYPI SATTPHNFAT TTFQTPTFCY ECEGLLWGLA 730 740 750 760 770 780 RQGLRCTQCQ VKVHDKCREL LSADCLQRAA EKSTKHGEAD RTQSLVNVIR DRMKIQEQNK 790 800 810 820 830 840 PEVFQMIRTV FDVDENIQKE TLKTVKASIL EGSSKWSAKI TLTVLCAQGL IAKDKTGKSD 850 860 870 880 890 900 PYVTAQVGKT KRRTRTIHQE LNPVWNEKFH FECHNSTDRI KVRVWDEDND LKSKLRQKLT 910 920 930 940 950 960 RESDDFLGQT VIEVRTLSGE MDVWYNLEKR TDKSAVSGAI RLHINVEIKG EEKLAPYHVQ 970 980 990 1000 1010 1020 YTCLHEHLFA AHCVDEEVKL PKVRGEDSWK VCFQETGQEI AEEFAMRYGI ESIYQAMTHF 1030 1040 1050 1060 1070 1080 ACLCTRYMCA GVPAVLSTLL ANINAYYAHT TATSAVSAPD RFAASNFGKE RFVKLLDQLH 1090 1100 1110 1120 1130 1140 NSLRIDLSAY RNHFPSSSPA KLQDLKSTVD LLTSITFFRM KVLELASPPR ASTVVRECAK 1150 1160 1170 1180 1190 1200 ACMQQTYQLM FESCAEQFPI LDTSVQFWYE FIDYIMRVIE EDQKNYTPAL NQFPQELNVG 1210 1220 1230 1240 1250 1260 NLSAETLWSM YKNDLKMALE EHAQKKRCKT PEYMNLYFKV KGFYFKYVAD LSTYKSSIPE 1270 1280 1290 1300 1310 1320 FPAWFIPFVM DWLNENDEHS MDILRNAYNC RQADTSHKHQ NTQILEHVVD VFTQLNAALK 1330 1340 1350 1360 1370 1380 LLKQMDCPNP EVAADMMKRF SKTLNKVLLA YADMVQKDFP KFAHDEKLAC ILMNNVQQLR 1390 1400 1410 1420 1430 1440 VQLEKIYETM GGAELDEHIG QVLTVLQKKL NSVLDRLSAE FVTTLEPHIH EQTIKLGMLL 1450 1460 1470 1480 1490 1500 VKIKGPQLQK TQVQPEADAV LEPLMDLLEG SLRRYADQCE KTVLKYILKE LWKITIVNME 1510 1520 1530 1540 1550 1560 KRVVLPPLSD KALLKQLPNA KIGDVTKLMS TNIQSIKGMN SVKDMMDMAR ESEKSLTPRQ 1570 1580 1590 1600 1610 1620 CTVLDCALDA IKDSFHASGK GLKKSFFEKS PELQSLKYAL SLYTQTTEQL IKTFITSQRQ 1630 1640 1650 1660 1670 1680 QDLPSQEQPV GEVSVQVDLF SHPGTGEQKV TVKILAANDL RWQTSSAFKP FVEVHLVGPH 1690 1700 1710 1720 1730 1740 LSDKKRKWST KTKAGNWAPK FNETFHFFLG NEGEPEHYEL MFQVKDYCFA RDDRVVGVGV 1750 1760 1770 1780 1790 1800 LQLSSVVDQA GSCAMWVQLG TRLHIDETGL ILLRILSQRQ TDEVAKDFVR LKTECRYETE 1810 TVMAASASSQ NINRT
	Isoform 3 [UniParc]. Checksum: EB86DDE71B5D98AF	FASTA	2,140	243,591
	10 20 30 40 50 60 MSMRLLCITI KKARLQGAVD EFNSYVTVKL QTVKSTTVAV RGNLPCWEQE FIFETNRPDD 70 80 90 100 110 120 GMVLELWAKG VLWDKLIGVH YMPLSEIRYS NAAGSGQWLQ MDHELETRNG QTVGTRGPTG 130 140 150 160 170 180 HNLLTDVRFE LPFDVQGHDE DIQSRLLALN GLIEHDQLGP NNHHRAPFNH SGLSEDSDYT 190 200 210 220 230 240 SDVSVPVNHH QLHPNSSAHQ YESHLHPHRT RQLLHTREGA ASYEDEEDAY HARHQPESDD 250 260 270 280 290 300 YNHQDTYDQH SSYYNDEYAP SGSSSQYQRQ GYQDQDQQHQ NIYEDTVTPV REFGESAVPP 310 320 330 340 350 360 AASSSRRQFD QVYGYASSSE ERYDTPMSSG RLPRDEPILE HSEPEYVYDQ NGYPEEDNYG 370 380 390 400 410 420 INPTYSEDHF EGQTNDYSTT HQEPNDFRND YNSSYQREYW NESEPLSYNS RPPNGHIRTG 430 440 450 460 470 480 ANTWREPSTS SRPTSSQAWN YQDDTHQYDE VDRGSRVSFT RTPSVDRTDR PSESGGGFYD 490 500 510 520 530 540 EMSESGRPGR PDSHHNWRYD SIQEEDNEKD NWKQHVEGYE EGQEEKQKDN QKPNDHSAAS 550 560 570 580 590 600 PQDHYHRSDS TAQQDFGNNI VRQTIQEEEE KRNYQELWHN AYKRVCADLG IKSSQASLVA 610 620 630 640 650 660 TTGGTLLQNL FLYRPKLAPA GHGATASQFQ SPAGATRFYA NHNNNNNNVS KNELDSTTET 670 680 690 700 710 720 PPDPSRTPST SSMNPVPSLA VPMSPGPYLN SDPPSPVSPN PQIKRSIYRI KESYEDRNGG 730 740 750 760 770 780 RERIYTTNLV SVYLEKMKPP DELEEGSSGS MRETQNEIKN GTQLHNAESN IFFPQDSVPK 790 800 810 820 830 840 SISYNAGNLK NTSITTSKTS SAITNHSSLP PQPPSKPASR DSDPMKQLLT FSKSFKKVRR 850 860 870 880 890 900 VRSAMPRRRK RKRVKIKKSR SCPILWKTEK TPHPMKSKSM TCIRIPKKTV IAPLRKEIKI 910 920 930 940 950 960 VRMKPPAARC ESDSKAHKKK NLLDVYKDMG KSTVLDGNGS SAANAFYKSI DAAPNMNVAR 970 980 990 1000 1010 1020 TKTSIPLVSE LVLKTMATKR AQAGLANAAR TTFSDTELKT HVYKKTLQAL IYPISATTPH 1030 1040 1050 1060 1070 1080 NFATTTFQTP TFCYECEGLL WGLARQGLRC TQCQVKVHDK CRELLSADCL QRAAEKSTKH 1090 1100 1110 1120 1130 1140 GEADRTQSLV NVIRDRMKIQ EQNKPEVFQM IRTVFDVDEN IQKETLKTVK ASILEGSSKW 1150 1160 1170 1180 1190 1200 SAKITLTVLC AQGLIAKDKT GKSDPYVTAQ VGKTKRRTRT IHQELNPVWN EKFHFECHNS 1210 1220 1230 1240 1250 1260 TDRIKVRVWD EDNDLKSKLR QKLTRESDDF LGQTVIEVRT LSGEMDVWYN LEKRTDKSAV 1270 1280 1290 1300 1310 1320 SGAIRLHINV EIKGEEKLAP YHVQYTCLHE HLFAAHCVDE EVKLPKVRGE DSWKVCFQET 1330 1340 1350 1360 1370 1380 GQEIAEEFAM RYGIESIYQA MTHFACLCTR YMCAGVPAVL STLLANINAY YAHTTATSAV 1390 1400 1410 1420 1430 1440 SAPDRFAASN FGKERFVKLL DQLHNSLRID LSAYRNHFPS SSPAKLQDLK STVDLLTSIT 1450 1460 1470 1480 1490 1500 FFRMKVLELA SPPRASTVVR ECAKACMQQT YQLMFESCAE QFPILDTSVQ FWYEFIDYIM 1510 1520 1530 1540 1550 1560 RVIEEDQKNY TPALNQFPQE LNVGNLSAET LWSMYKNDLK MALEEHAQKK RCKTPEYMNL 1570 1580 1590 1600 1610 1620 YFKVKGFYFK YVADLSTYKS SIPEFPAWFI PFVMDWLNEN DEHSMDILRN AYNVDKADNF 1630 1640 1650 1660 1670 1680 PQTSEHTKFS NSVVDVFTQL NAALKLLKQM DCPNPEVAAD MMKRFSKTLN KVLLAYADMV 1690 1700 1710 1720 1730 1740 QKDFPKFAHD EKLACILMNN VQQLRVQLEK IYETMGGAEL DEHIGQVLTV LQKKLNSVLD 1750 1760 1770 1780 1790 1800 RLSAEFVTTL EPHIHEQTIK LGMLLVKIKG PQLQKTQVQP EADAVLEPLM DLLEGSLRRY 1810 1820 1830 1840 1850 1860 ADQCEKTVLK YILKELWKIT IVNMEKRVVL PPLSDKALLK QLPNAKIGDV TKLMSTNIQS 1870 1880 1890 1900 1910 1920 IKGMNSVKDM MDMARESEKS LTPRQCTVLD CALDAIKDSF HASGKGLKKS FFEKSPELQS 1930 1940 1950 1960 1970 1980 LKYALSLYTQ TTEQLIKTFI TSQRQQDLPS QEQPVGEVSV QVDLFSHPGT GEQKVTVKIL 1990 2000 2010 2020 2030 2040 AANDLRWQTS SAFKPFVEVH LVGPHLSDKK RKWSTKTKAG NWAPKFNETF HFFLGNEGEP 2050 2060 2070 2080 2090 2100 EHYELMFQVK DYCFARDDRV VGVGVLQLSS VVDQAGSCAM WVQLGTRLHI DETGLILLRI 2110 2120 2130 2140 LSQRQTDEVA KDFVRLKTEC RYETETVMAA SASSQNINRT
	Isoform 5 (c) [UniParc]. Checksum: 654E35E22754B310	FASTA	1,475	168,697
	10 20 30 40 50 60 MSSGRLPRDE PILEHSEPEY VYDQNGYPEE DNYGINPTYS EDHFEGQTND YSTTHQEPND 70 80 90 100 110 120 FRNDYNSSYQ REYWNESEPL SYNSRPPNGH IRTGANTWRE PSTSSRPTSS QAWNYQDDTH 130 140 150 160 170 180 QYDEVDRGSR VSFTRTPSVD RTDRPSESGG GFYDEMSESG RPGRPDSHHN WRYDSIQEED 190 200 210 220 230 240 NEKDNWKQHV EGYEEGQEEK QKDNQKPNDH SAASPQDHYH RSDSTAQQDF GNNIVRQTIQ 250 260 270 280 290 300 EEEEKRNYQE LWHNAYKRVC ADLGIKSTVL DGNGSSAANA FYKSIDAAPN MNVARTKTSI 310 320 330 340 350 360 PLVSELVLKT MATKRAQAGL ANAARTTFSD TELKTHVYKK TLQALIYPIS ATTPHNFATT 370 380 390 400 410 420 TFQTPTFCYE CEGLLWGLAR QGLRCTQCQV KVHDKCRELL SADCLQRAAE KSTKHGEADR 430 440 450 460 470 480 TQSLVNVIRD RMKIQEQNKP EVFQMIRTVF DVDENIQKET LKTVKASILE GSSKWSAKIT 490 500 510 520 530 540 LTVLCAQGLI AKDKTGKSDP YVTAQVGKTK RRTRTIHQEL NPVWNEKFHF ECHNSTDRIK 550 560 570 580 590 600 VRVWDEDNDL KSKLRQKLTR ESDDFLGQTV IEVRTLSGEM DVWYNLEKRT DKSAVSGAIR 610 620 630 640 650 660 LHINVEIKGE EKLAPYHVQY TCLHEHLFAA HCVDEEVKLP KVRGEDSWKV CFQETGQEIA 670 680 690 700 710 720 EEFAMRYGIE SIYQAMTHFA CLCTRYMCAG VPAVLSTLLA NINAYYAHTT ATSAVSAPDR 730 740 750 760 770 780 FAASNFGKER FVKLLDQLHN SLRIDLSAYR NHFPSSSPAK LQDLKSTVDL LTSITFFRMK 790 800 810 820 830 840 VLELASPPRA STVVRECAKA CMQQTYQLMF ESCAEQFPIL DTSVQFWYEF IDYIMRVIEE 850 860 870 880 890 900 DQKNYTPALN QFPQELNVGN LSAETLWSMY KNDLKMALEE HAQKKRCKTP EYMNLYFKVK 910 920 930 940 950 960 GFYFKYVADL STYKSSIPEF PAWFIPFVMD WLNENDEHSM DILRNAYNVD KADNFPQTSE 970 980 990 1000 1010 1020 HTKFSNSVVD VFTQLNAALK LLKQMDCPNP EVAADMMKRF SKTLNKVLLA YADMVQKDFP 1030 1040 1050 1060 1070 1080 KFAHDEKLAC ILMNNVQQLR VQLEKIYETM GGAELDEHIG QVLTVLQKKL NSVLDRLSAE 1090 1100 1110 1120 1130 1140 FVTTLEPHIH EQTIKLGMLL VKIKGPQLQK TQVQPEADAV LEPLMDLLEG SLRRYADQCE 1150 1160 1170 1180 1190 1200 KTVLKYILKE LWKITIVNME KRVVLPPLSD KALLKQLPNA KIGDVTKLMS TNIQSIKGMN 1210 1220 1230 1240 1250 1260 SVKDMMDMAR ESEKSLTPRQ CTVLDCALDA IKDSFHASGK GLKKSFFEKS PELQSLKYAL 1270 1280 1290 1300 1310 1320 SLYTQTTEQL IKTFITSQRQ QDLPSQEQPV GEVSVQVDLF SHPGTGEQKV TVKILAANDL 1330 1340 1350 1360 1370 1380 RWQTSSAFKP FVEVHLVGPH LSDKKRKWST KTKAGNWAPK FNETFHFFLG NEGEPEHYEL 1390 1400 1410 1420 1430 1440 MFQVKDYCFA RDDRVVGVGV LQLSSVVDQA GSCAMWVQLG TRLHIDETGL ILLRILSQRQ 1450 1460 1470 TDEVAKDFVR LKTECRYETE TVMAASASSQ NINRT
	Isoform 7 (e) [UniParc]. Checksum: 65DEC63A1157E67D	FASTA	1,816	207,591
	10 20 30 40 50 60 MDDVGDYNDD QLSDGMSMRL LCITIKKARL QGAVDEFNSY VTVKLQTVKS TTVAVRGNLP 70 80 90 100 110 120 CWEQEFIFET NRPDDGMVLE LWAKGVLWDK LIGVHYMPLS EIRYSNAAGS GQWLQMDHEL 130 140 150 160 170 180 ETRNGQTVGT RGPTGHNLLT DVRFELPFDV QGHDEDIQSR LLALNGLIEH DQLGPNNHHR 190 200 210 220 230 240 APFNHSGLSE DSDYTSDVSV PVNHHQLHPN SSAHQYESHL HPHRTRQLLH TREGAASYED 250 260 270 280 290 300 EEDAYHARHQ PESDDYNHQD TYDQHSSYYN DEYAPSGSSS QYQRQGYQDQ DQQHQNIYED 310 320 330 340 350 360 TVTPVREFGE SAVPPAASSS RRQFDQVYGY ASSSEERYDT PMSSGRLPRD EPILEHSEPE 370 380 390 400 410 420 YVYDQNGYPE EDNYGINPTY SEDHFEGQTN DYSTTHQEPN DFRNDYNSSY QREYWNESEP 430 440 450 460 470 480 LSYNSRPPNG HIRTGANTWR EPSTSSRPTS SQAWNYQDDT HQYDEVDRGS RVSFTRTPSV 490 500 510 520 530 540 DRTDRPSESG GGFYDEMSES GRPGRPDSHH NWRYDSIQEE DNEKDNWKQH VEGYEEGQEE 550 560 570 580 590 600 KQKDNQKPND HSAASPQDHY HRSDSTAQQD FGNNIVRQTI QEEEEKRNYQ ELWHNAYKRV 610 620 630 640 650 660 CADLGIKSTV LDGNGSSAAN AFYKSIDAAP NMNVARTKTS IPLVSELVLK TMATKRAQAG 670 680 690 700 710 720 LANAARTTFS DTELKTHVYK KTLQALIYPI SATTPHNFAT TTFQTPTFCY ECEGLLWGLA 730 740 750 760 770 780 RQGLRCTQCQ VKVHDKCREL LSADCLQRAA EKSTKHGEAD RTQSLVNVIR DRMKIQEQNK 790 800 810 820 830 840 PEVFQMIRTV FDVDENIQKE TLKTVKASIL EGSSKWSAKI TLTVLCAQGL IAKDKTGKSD 850 860 870 880 890 900 PYVTAQVGKT KRRTRTIHQE LNPVWNEKFH FECHNSTDRI KVRVWDEDND LKSKLRQKLT 910 920 930 940 950 960 RESDDFLGQT VIEVRTLSGE MDVWYNLEKR TDKSAVSGAI RLHINVEIKG EEKLAPYHVQ 970 980 990 1000 1010 1020 YTCLHEHLFA AHCVDEEVKL PKVRGEDSWK VCFQETGQEI AEEFAMRYGI ESIYQAMTHF 1030 1040 1050 1060 1070 1080 ACLCTRYMCA GVPAVLSTLL ANINAYYAHT TATSAVSAPD RFAASNFGKE RFVKLLDQLH 1090 1100 1110 1120 1130 1140 NSLRIDLSAY RNHFPSSSPA KLQDLKSTVD LLTSITFFRM KVLELASPPR ASTVVRECAK 1150 1160 1170 1180 1190 1200 ACMQQTYQLM FESCAEQFPI LDTSVQFWYE FIDYIMRVIE EDQKNYTPAL NQFPQELNVG 1210 1220 1230 1240 1250 1260 NLSAETLWSM YKNDLKMALE EHAQKKRCKT PEYMNLYFKV KGFYFKYVAD LSTYKSSIPE 1270 1280 1290 1300 1310 1320 FPAWFIPFVM DWLNENDEHS MDILRNAYNV DKADNFPQTS EHTKFSNSVV DVFTQLNAAL 1330 1340 1350 1360 1370 1380 KLLKQMDCPN PEVAADMMKR FSKTLNKVLL AYADMVQKDF PKFAHDEKLA CILMNNVQQL 1390 1400 1410 1420 1430 1440 RVQLEKIYET MGGAELDEHI GQVLTVLQKK LNSVLDRLSA EFVTTLEPHI HEQTIKLGML 1450 1460 1470 1480 1490 1500 LVKIKGPQLQ KTQVQPEADA VLEPLMDLLE GSLRRYADQC EKTVLKYILK ELWKITIVNM 1510 1520 1530 1540 1550 1560 EKRVVLPPLS DKALLKQLPN AKIGDVTKLM STNIQSIKGM NSVKDMMDMA RESEKSLTPR 1570 1580 1590 1600 1610 1620 QCTVLDCALD AIKDSFHASG KGLKKSFFEK SPELQSLKYA LSLYTQTTEQ LIKTFITSQR 1630 1640 1650 1660 1670 1680 QQDLPSQEQP VGEVSVQVDL FSHPGTGEQK VTVKILAAND LRWQTSSAFK PFVEVHLVGP 1690 1700 1710 1720 1730 1740 HLSDKKRKWS TKTKAGNWAP KFNETFHFFL GNEGEPEHYE LMFQVKDYCF ARDDRVVGVG 1750 1760 1770 1780 1790 1800 VLQLSSVVDQ AGSCAMWVQL GTRLHIDETG LILLRILSQR QTDEVAKDFV RLKTECRYET 1810 ETVMAASASS QNINRT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A phorbol ester/diacylglycerol-binding protein encoded by the unc-13 gene of Caenorhabditis elegans." Maruyama I.N., Brenner S. Proc. Natl. Acad. Sci. U.S.A. 88:5729-5733(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), DISRUPTION PHENOTYPE. Strain: Bristol N2.
[2]	"Expression of multiple UNC-13 proteins in the Caenorhabditis elegans nervous system." Kohn R.E., Duerr J.S., McManus J.R., Duke A., Rakow T.L., Maruyama H., Moulder G., Maruyama I.N., Barstead R.J., Rand J.B. Mol. Biol. Cell 11:3441-3452(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PARTIAL NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1/2/6/7 AND 3). Strain: Bristol N2.
[3]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING. Strain: Bristol N2.
[4]	"The Caenorhabditis elegans unc-13 gene product is a phospholipid-dependent high-affinity phorbol ester receptor." Ahmed S., Maruyama I.N., Kozma R., Lee J., Brenner S., Lim L. Biochem. J. 287:995-999(1992) [PubMed] [Europe PMC] [Abstract] Cited for: ZINC AND PHORBOL-ESTERS BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M62830 mRNA. Translation: AAA93094.1. U50735 Genomic DNA. Translation: AAA99780.1. U50735 Genomic DNA. Translation: AAA99781.1. Z73912, Z79694, Z92779 Genomic DNA. Translation: CAA98147.1. Z73912, Z92779 Genomic DNA. Translation: CAD56619.2. Z73912, Z79694, Z92779 Genomic DNA. Translation: CAD90190.2. Z73912, Z79694, Z92779 Genomic DNA. Translation: CAE11317.1. Z79694, Z92779, Z73912 Genomic DNA. Translation: CAB01966.1. Z79694, Z73912, Z92779 Genomic DNA. Translation: CAD90170.2. Z79694, Z73912, Z92779 Genomic DNA. Translation: CAE11299.1. Z92779, Z79694, Z73912 Genomic DNA. Translation: CAB07173.1. Z92779, Z73912 Genomic DNA. Translation: CAD56561.2. Z92779, Z73912, Z79694 Genomic DNA. Translation: CAD90173.2. Z92779, Z73912, Z79694 Genomic DNA. Translation: CAE11294.1.
PIR	A41101. T19295. T27886.
RefSeq	NP_001021871.1. NM_001026700.1. NP_001021872.1. NM_001026701.1. NP_001021873.1. NM_001026702.1. NP_001021874.1. NM_001026703.2.
UniGene	Cel.16847.
3D structure databases
ProteinModelPortal	P27715.
SMR	P27715. Positions 20-145, 1006-1287, 1602-1945.
ModBase	Search...
Proteomic databases
PaxDb	P27715.
PRIDE	P27715.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	ZK524.2d; ZK524.2d; ZK524.2.
GeneID	172497.
KEGG	cel:CELE_ZK524.2.
UCSC	ZK524.2e. c. elegans.
Organism-specific databases
CTD	43841.
WormBase	ZK524.2a; CE15371; WBGene00006752; unc-13. ZK524.2c; CE34624; WBGene00006752; unc-13. ZK524.2d; CE34625; WBGene00006752; unc-13. ZK524.2e; CE34626; WBGene00006752; unc-13.
Phylogenomic databases
eggNOG	NOG245727.
GeneTree	ENSGT00680000099818.
HOGENOM	HOG000231404.
InParanoid	P27715.
OMA	ILWKTEK.
Gene expression databases
ArrayExpress	P27715.
Family and domain databases
InterPro	IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR020477. C2_dom. IPR018029. C2_membr_targeting. IPR010439. Ca-dep_secretion_activator. IPR020454. DAG/PE-bd. IPR014770. Munc13_1. IPR014772. Munc13_dom-2. IPR019558. Munc13_subgr_dom-2. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. IPR027080. Unc-13. [Graphical view]
PANTHER	PTHR10480. PTHR10480. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00168. C2. 3 hits. PF06292. DUF1041. 1 hit. PF10540. Membr_traf_MHD. 1 hit. [Graphical view]
PRINTS	PR00360. C2DOMAIN. PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00239. C2. 3 hits. [Graphical view]
SUPFAM	SSF49562. C2_CaLB. 3 hits.
PROSITE	PS50004. C2. 2 hits. PS51258. MHD1. 1 hit. PS51259. MHD2. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	875765.

Entry information

Entry name

UNC13_CAEEL

Accession

Primary (citable) accession number: P27715
Secondary accession number(s): O17665 , Q23512, Q7YSR2, Q867Z2, Q8I095

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	March 29, 2004
Last modified:	May 1, 2013
This is version 117 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project