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P27710 (TRPG_CRYNH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multifunctional tryptophan biosynthesis protein

Including the following 3 domains:

  1. Anthranilate synthase component 2
    Short name=AS
    EC=4.1.3.27
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
  2. Indole-3-glycerol phosphate synthase
    Short name=IGPS
    EC=4.1.1.48
  3. N-(5'-phosphoribosyl)anthranilate isomerase
    Short name=PRAI
    EC=5.3.1.24
Gene names
Name:TRP1
ORF Names:CNAG_04501
OrganismCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii) [Complete proteome]
Taxonomic identifier235443 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex

Protein attributes

Sequence length752 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities. HAMAP-Rule MF_00135

Catalytic activity

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate. HAMAP-Rule MF_00135

1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O. HAMAP-Rule MF_00135

Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate. HAMAP-Rule MF_00135

Pathway

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5. HAMAP-Rule MF_00135

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 3/5.

Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 4/5.

Sequence similarities

Contains 1 glutamine amidotransferase type-1 domain.

Sequence caution

The sequence AAA51445.1 differs from that shown. Reason: Erroneous termination at position 324. Translated as Glu.

The sequence AAA51445.1 differs from that shown. Reason: Frameshift at positions 297, 302, 307, 310 and 716.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 752752Multifunctional tryptophan biosynthesis protein HAMAP-Rule MF_00135
PRO_0000056858

Regions

Domain3 – 202200Glutamine amidotransferase type-1
Region231 – 495265Indole-3-glycerol phosphate synthase HAMAP-Rule MF_00135
Region509 – 752244N-(5'-phosphoribosyl)anthranilate isomerase HAMAP-Rule MF_00135

Sites

Active site861For GATase activity By similarity
Active site1761For GATase activity By similarity
Active site1781For GATase activity By similarity

Experimental info

Sequence conflict295 – 30915KGDIA…PQQAL → FQGRNAPTLLLLRST Ref.2
Sequence conflict5041P → S in AAA51445. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P27710 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: FE31FEF7BFC5FC91

FASTA75280,194
        10         20         30         40         50         60 
MGFTLLIDNY DSFTWNIYAD LASVGGNPFV VRNDKITLKE IEGMFADGEL ERIVISPGPG 

        70         80         90        100        110        120 
HPRTDSGVSR DVIAWGMGKL PILGVCMGLE CIVDLLGGEI AYAGEIKHGK TSLVQHDSIG 

       130        140        150        160        170        180 
VFHNLPQFLS STRYHSLSAQ IQSLPSVLQV TSTTKESGVI MGVRHRTFTV EAVQYHPESC 

       190        200        210        220        230        240 
MSEGGRGLMA NFIQMKGGKW GGENAWCGVP AEGEGEQPKA KTNGAPSLPT ILNKIHAQRL 

       250        260        270        280        290        300 
LDVEQAEKIP ATTPANVSTS LSLYTSPPLI NFRGRMVSTP HTAVMAEIKR ASPSKGDIAP 

       310        320        330        340        350        360 
TASAPQQALK YALAGASVIS VLTEPTWFKG SLLDMLAVRN AVDSLPNRPA ILRKDFVLSK 

       370        380        390        400        410        420 
YMIDEARLYG ADTVLLIVAM LEPQQLKELY DYSVSLGMEP LVEVNNPTEL SLALEIGSKV 

       430        440        450        460        470        480 
IGVNNRNLHD FNVDMSTTSR VNAALNGRDV VLCALSGISS HEDVEKYVKE GVKGVLVGEA 

       490        500        510        520        530        540 
LMRASDTKAF LRSLIGLPPL EVVPKPRPLV KICGIRSTND AKLAINAGAD LLGVILVPGT 

       550        560        570        580        590        600 
KRCISTSTAR EISALVQSAR SQSSSKPLEP SLSSPWFTSQ SALLSSRRKP LLVGVFQNQS 

       610        620        630        640        650        660 
LSDILSAVDE IGLDLVQLHG DEPQAWAKFI PVPVVKVFRV SPEGIVRGGE IRRPGLNQAI 

       670        680        690        700        710        720 
LLDAGGASGG GGEGKAFPWE HAKRLIQSGE VGSEGHVPLP VILAGGLTPE NVGQAIEQAG 

       730        740        750 
EGVWCVDVSS GVEGEGGKVK EKVEAFVKAV RG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP003828 Genomic DNA. Translation: AFR97220.1.
M74901 Genomic DNA. Translation: AAA51445.1. Sequence problems.
PIRJN0451.

3D structure databases

ProteinModelPortalP27710.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

OrthoDBEOG78WM1Q.

Enzyme and pathway databases

UniPathwayUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
3.40.50.880. 1 hit.
HAMAPMF_00135. PRAI.
InterProIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsTIGR00566. trpG_papA. 1 hit.
PROSITEPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRPG_CRYNH
AccessionPrimary (citable) accession number: P27710
Secondary accession number(s): J9VZN1
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 21, 2005
Last modified: June 11, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways