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P27710

- TRPG_CRYNH

UniProt

P27710 - TRPG_CRYNH

Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRP1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 90 (01 Oct 2014)
      Sequence version 2 (21 Jun 2005)
      Previous versions | rss
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    Functioni

    Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

    Catalytic activityi

    N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
    1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
    Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei86 – 861For GATase activityBy similarity
    Active sitei176 – 1761For GATase activityBy similarity
    Active sitei178 – 1781For GATase activityBy similarity

    GO - Molecular functioni

    1. anthranilate synthase activity Source: UniProtKB-EC
    2. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
    3. phosphoribosylanthranilate isomerase activity Source: UniProtKB-EC
    4. transferase activity Source: UniProtKB-KW

    GO - Biological processi

    1. glutamine metabolic process Source: UniProtKB-KW
    2. tryptophan biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Decarboxylase, Isomerase, Lyase, Transferase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00035; UER00040.
    UPA00035; UER00042.
    UPA00035; UER00043.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multifunctional tryptophan biosynthesis protein
    Including the following 3 domains:
    Anthranilate synthase component 2 (EC:4.1.3.27)
    Short name:
    AS
    Alternative name(s):
    Anthranilate synthase, glutamine amidotransferase component
    Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
    Short name:
    IGPS
    N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
    Short name:
    PRAI
    Gene namesi
    Name:TRP1
    ORF Names:CNAG_04501
    OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
    Taxonomic identifieri235443 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
    ProteomesiUP000010091: Chromosome 9

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 752752Multifunctional tryptophan biosynthesis proteinPRO_0000056858Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP27710.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 202200Glutamine amidotransferase type-1Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni231 – 495265Indole-3-glycerol phosphate synthaseAdd
    BLAST
    Regioni509 – 752244N-(5'-phosphoribosyl)anthranilate isomeraseAdd
    BLAST

    Sequence similaritiesi

    Keywords - Domaini

    Glutamine amidotransferase

    Phylogenomic databases

    OrthoDBiEOG78WM1Q.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPiMF_00135. PRAI.
    InterProiIPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view]
    PfamiPF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMiSSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
    PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27710-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFTLLIDNY DSFTWNIYAD LASVGGNPFV VRNDKITLKE IEGMFADGEL    50
    ERIVISPGPG HPRTDSGVSR DVIAWGMGKL PILGVCMGLE CIVDLLGGEI 100
    AYAGEIKHGK TSLVQHDSIG VFHNLPQFLS STRYHSLSAQ IQSLPSVLQV 150
    TSTTKESGVI MGVRHRTFTV EAVQYHPESC MSEGGRGLMA NFIQMKGGKW 200
    GGENAWCGVP AEGEGEQPKA KTNGAPSLPT ILNKIHAQRL LDVEQAEKIP 250
    ATTPANVSTS LSLYTSPPLI NFRGRMVSTP HTAVMAEIKR ASPSKGDIAP 300
    TASAPQQALK YALAGASVIS VLTEPTWFKG SLLDMLAVRN AVDSLPNRPA 350
    ILRKDFVLSK YMIDEARLYG ADTVLLIVAM LEPQQLKELY DYSVSLGMEP 400
    LVEVNNPTEL SLALEIGSKV IGVNNRNLHD FNVDMSTTSR VNAALNGRDV 450
    VLCALSGISS HEDVEKYVKE GVKGVLVGEA LMRASDTKAF LRSLIGLPPL 500
    EVVPKPRPLV KICGIRSTND AKLAINAGAD LLGVILVPGT KRCISTSTAR 550
    EISALVQSAR SQSSSKPLEP SLSSPWFTSQ SALLSSRRKP LLVGVFQNQS 600
    LSDILSAVDE IGLDLVQLHG DEPQAWAKFI PVPVVKVFRV SPEGIVRGGE 650
    IRRPGLNQAI LLDAGGASGG GGEGKAFPWE HAKRLIQSGE VGSEGHVPLP 700
    VILAGGLTPE NVGQAIEQAG EGVWCVDVSS GVEGEGGKVK EKVEAFVKAV 750
    RG 752
    Length:752
    Mass (Da):80,194
    Last modified:June 21, 2005 - v2
    Checksum:iFE31FEF7BFC5FC91
    GO

    Sequence cautioni

    The sequence AAA51445.1 differs from that shown. Reason: Frameshift at positions 297, 302, 307, 310 and 716.
    The sequence AAA51445.1 differs from that shown. Reason: Erroneous termination at position 324. Translated as Glu.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti295 – 30915KGDIA…PQQAL → FQGRNAPTLLLLRST(PubMed:1452032)CuratedAdd
    BLAST
    Sequence conflicti504 – 5041P → S in AAA51445. (PubMed:1452032)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003828 Genomic DNA. Translation: AFR97220.1.
    M74901 Genomic DNA. Translation: AAA51445.1. Sequence problems.
    PIRiJN0451.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP003828 Genomic DNA. Translation: AFR97220.1 .
    M74901 Genomic DNA. Translation: AAA51445.1 . Sequence problems.
    PIRi JN0451.

    3D structure databases

    ProteinModelPortali P27710.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    OrthoDBi EOG78WM1Q.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00040 .
    UPA00035 ; UER00042 .
    UPA00035 ; UER00043 .

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    3.40.50.880. 1 hit.
    HAMAPi MF_00135. PRAI.
    InterProi IPR013785. Aldolase_TIM.
    IPR016302. Anthranilate_synth_II.
    IPR029062. Class_I_gatase-like.
    IPR017926. GATASE.
    IPR013798. Indole-3-glycerol_P_synth.
    IPR001468. Indole-3-GlycerolPSynthase_CS.
    IPR001240. PRAI_dom.
    IPR011060. RibuloseP-bd_barrel.
    IPR006221. TrpG/PapA_dom.
    [Graphical view ]
    Pfami PF00117. GATase. 1 hit.
    PF00218. IGPS. 1 hit.
    PF00697. PRAI. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
    SUPFAMi SSF51366. SSF51366. 3 hits.
    SSF52317. SSF52317. 1 hit.
    TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
    PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
    PS00614. IGPS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
      Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
      , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
      PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
    2. "Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae."
      Perfect J.R., Rude T.H., Penning L.M., Johnston S.A.
      Gene 122:213-217(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 294-752.

    Entry informationi

    Entry nameiTRPG_CRYNH
    AccessioniPrimary (citable) accession number: P27710
    Secondary accession number(s): J9VZN1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: June 21, 2005
    Last modified: October 1, 2014
    This is version 90 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3