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Protein

Multifunctional tryptophan biosynthesis protein

Gene

TRP1

Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.

Pathwayi: L-tryptophan biosynthesis

This protein is involved in step 1, 3 and 4 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Multifunctional tryptophan biosynthesis protein (TRP1)
  2. no protein annotated in this organism
  3. Multifunctional tryptophan biosynthesis protein (TRP1)
  4. Multifunctional tryptophan biosynthesis protein (TRP1)
  5. Tryptophan synthase (CNAG_00649)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei86Nucleophile; for GATase activityBy similarity1
Active sitei176For GATase activityBy similarity1
Active sitei178For GATase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Isomerase, Lyase, Multifunctional enzyme
Biological processAmino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040
UPA00035; UER00042
UPA00035; UER00043

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:TRP1
ORF Names:CNAG_04501
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesCryptococcaceaeCryptococcusCryptococcus neoformans species complex
Proteomesi
  • UP000010091 Componenti: Chromosome 9

Organism-specific databases

EuPathDBiFungiDB:CNAG_04501

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000568581 – 752Multifunctional tryptophan biosynthesis proteinAdd BLAST752

Proteomic databases

PRIDEiP27710

Structurei

3D structure databases

ProteinModelPortaliP27710
SMRiP27710
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 202Glutamine amidotransferase type-1Add BLAST200

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni58 – 60Glutamine bindingBy similarity3
Regioni136 – 137Glutamine bindingBy similarity2
Regioni231 – 495Indole-3-glycerol phosphate synthaseAdd BLAST265
Regioni509 – 752N-(5'-phosphoribosyl)anthranilate isomeraseAdd BLAST244

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

OrthoDBiEOG092C1PKC

Family and domain databases

CDDicd00331 IGPS, 1 hit
cd00405 PRAI, 1 hit
Gene3Di3.20.20.70, 3 hits
3.40.50.880, 1 hit
HAMAPiMF_00135 PRAI, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR016302 Anthranilate_synth_II
IPR029062 Class_I_gatase-like
IPR017926 GATASE
IPR013798 Indole-3-glycerol_P_synth
IPR001468 Indole-3-GlycerolPSynthase_CS
IPR001240 PRAI
IPR011060 RibuloseP-bd_barrel
IPR006221 TrpG/PapA_dom
PfamiView protein in Pfam
PF00117 GATase, 1 hit
PF00218 IGPS, 1 hit
PF00697 PRAI, 1 hit
PIRSFiPIRSF001382 TrpG-trpC-trpF, 1 hit
SUPFAMiSSF51366 SSF51366, 3 hits
SSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR00566 trpG_papA, 1 hit
PROSITEiView protein in PROSITE
PS51273 GATASE_TYPE_1, 1 hit
PS00614 IGPS, 1 hit

Sequencei

Sequence statusi: Complete.

P27710-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFTLLIDNY DSFTWNIYAD LASVGGNPFV VRNDKITLKE IEGMFADGEL
60 70 80 90 100
ERIVISPGPG HPRTDSGVSR DVIAWGMGKL PILGVCMGLE CIVDLLGGEI
110 120 130 140 150
AYAGEIKHGK TSLVQHDSIG VFHNLPQFLS STRYHSLSAQ IQSLPSVLQV
160 170 180 190 200
TSTTKESGVI MGVRHRTFTV EAVQYHPESC MSEGGRGLMA NFIQMKGGKW
210 220 230 240 250
GGENAWCGVP AEGEGEQPKA KTNGAPSLPT ILNKIHAQRL LDVEQAEKIP
260 270 280 290 300
ATTPANVSTS LSLYTSPPLI NFRGRMVSTP HTAVMAEIKR ASPSKGDIAP
310 320 330 340 350
TASAPQQALK YALAGASVIS VLTEPTWFKG SLLDMLAVRN AVDSLPNRPA
360 370 380 390 400
ILRKDFVLSK YMIDEARLYG ADTVLLIVAM LEPQQLKELY DYSVSLGMEP
410 420 430 440 450
LVEVNNPTEL SLALEIGSKV IGVNNRNLHD FNVDMSTTSR VNAALNGRDV
460 470 480 490 500
VLCALSGISS HEDVEKYVKE GVKGVLVGEA LMRASDTKAF LRSLIGLPPL
510 520 530 540 550
EVVPKPRPLV KICGIRSTND AKLAINAGAD LLGVILVPGT KRCISTSTAR
560 570 580 590 600
EISALVQSAR SQSSSKPLEP SLSSPWFTSQ SALLSSRRKP LLVGVFQNQS
610 620 630 640 650
LSDILSAVDE IGLDLVQLHG DEPQAWAKFI PVPVVKVFRV SPEGIVRGGE
660 670 680 690 700
IRRPGLNQAI LLDAGGASGG GGEGKAFPWE HAKRLIQSGE VGSEGHVPLP
710 720 730 740 750
VILAGGLTPE NVGQAIEQAG EGVWCVDVSS GVEGEGGKVK EKVEAFVKAV

RG
Length:752
Mass (Da):80,194
Last modified:June 21, 2005 - v2
Checksum:iFE31FEF7BFC5FC91
GO

Sequence cautioni

The sequence AAA51445 differs from that shown. Reason: Frameshift at positions 297, 302, 307, 310 and 716.Curated
The sequence AAA51445 differs from that shown. Reason: Erroneous termination at position 324. Translated as Glu.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti295 – 309KGDIA…PQQAL → FQGRNAPTLLLLRST (PubMed:1452032).CuratedAdd BLAST15
Sequence conflicti504P → S in AAA51445 (PubMed:1452032).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP003828 Genomic DNA Translation: AFR97220.1
M74901 Genomic DNA Translation: AAA51445.1 Sequence problems.
PIRiJN0451
RefSeqiXP_012051839.1, XM_012196449.1

Genome annotation databases

EnsemblFungiiAFR97220; AFR97220; CNAG_04501
GeneIDi23887910

Similar proteinsi

Entry informationi

Entry nameiTRPG_CRYNH
AccessioniPrimary (citable) accession number: P27710
Secondary accession number(s): J9VZN1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 21, 2005
Last modified: May 23, 2018
This is version 107 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health