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P27710

- TRPG_CRYNH

UniProt

P27710 - TRPG_CRYNH

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Protein
Multifunctional tryptophan biosynthesis protein
Gene
TRP1, CNAG_04501
Organism
Cryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Trifunctional enzyme bearing the Gln amidotransferase (GATase) domain of anthranilate synthase, indole-glycerolphosphate synthase, and phosphoribosylanthranilate isomerase activities.UniRule annotation

Catalytic activityi

N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate.UniRule annotation
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate = 1-C-(3-indolyl)-glycerol 3-phosphate + CO2 + H2O.UniRule annotation
Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei86 – 861For GATase activity By similarity
Active sitei176 – 1761For GATase activity By similarity
Active sitei178 – 1781For GATase activity By similarity

GO - Molecular functioni

  1. anthranilate synthase activity Source: UniProtKB-EC
  2. indole-3-glycerol-phosphate synthase activity Source: UniProtKB-EC
  3. phosphoribosylanthranilate isomerase activity Source: UniProtKB-EC
  4. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. glutamine metabolic process Source: UniProtKB-KW
  2. tryptophan biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Isomerase, Lyase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

UniPathwayiUPA00035; UER00040.
UPA00035; UER00042.
UPA00035; UER00043.

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional tryptophan biosynthesis protein
Including the following 3 domains:
Anthranilate synthase component 2 (EC:4.1.3.27)
Short name:
AS
Alternative name(s):
Anthranilate synthase, glutamine amidotransferase component
Indole-3-glycerol phosphate synthase (EC:4.1.1.48)
Short name:
IGPS
N-(5'-phosphoribosyl)anthranilate isomerase (EC:5.3.1.24)
Short name:
PRAI
Gene namesi
Name:TRP1
ORF Names:CNAG_04501
OrganismiCryptococcus neoformans var. grubii serotype A (strain H99 / ATCC 208821 / CBS 10515 / FGSC 9487) (Filobasidiella neoformans var. grubii)
Taxonomic identifieri235443 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaTremellomycetesTremellalesTremellaceaeFilobasidiellaFilobasidiella/Cryptococcus neoformans species complex
ProteomesiUP000010091: Chromosome 9

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 752752Multifunctional tryptophan biosynthesis proteinUniRule annotation
PRO_0000056858Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP27710.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 202200Glutamine amidotransferase type-1
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni231 – 495265Indole-3-glycerol phosphate synthaseUniRule annotation
Add
BLAST
Regioni509 – 752244N-(5'-phosphoribosyl)anthranilate isomeraseUniRule annotation
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

OrthoDBiEOG78WM1Q.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
3.40.50.880. 1 hit.
HAMAPiMF_00135. PRAI.
InterProiIPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view]
PfamiPF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view]
PIRSFiPIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMiSSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsiTIGR00566. trpG_papA. 1 hit.
PROSITEiPS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27710-1 [UniParc]FASTAAdd to Basket

« Hide

MGFTLLIDNY DSFTWNIYAD LASVGGNPFV VRNDKITLKE IEGMFADGEL    50
ERIVISPGPG HPRTDSGVSR DVIAWGMGKL PILGVCMGLE CIVDLLGGEI 100
AYAGEIKHGK TSLVQHDSIG VFHNLPQFLS STRYHSLSAQ IQSLPSVLQV 150
TSTTKESGVI MGVRHRTFTV EAVQYHPESC MSEGGRGLMA NFIQMKGGKW 200
GGENAWCGVP AEGEGEQPKA KTNGAPSLPT ILNKIHAQRL LDVEQAEKIP 250
ATTPANVSTS LSLYTSPPLI NFRGRMVSTP HTAVMAEIKR ASPSKGDIAP 300
TASAPQQALK YALAGASVIS VLTEPTWFKG SLLDMLAVRN AVDSLPNRPA 350
ILRKDFVLSK YMIDEARLYG ADTVLLIVAM LEPQQLKELY DYSVSLGMEP 400
LVEVNNPTEL SLALEIGSKV IGVNNRNLHD FNVDMSTTSR VNAALNGRDV 450
VLCALSGISS HEDVEKYVKE GVKGVLVGEA LMRASDTKAF LRSLIGLPPL 500
EVVPKPRPLV KICGIRSTND AKLAINAGAD LLGVILVPGT KRCISTSTAR 550
EISALVQSAR SQSSSKPLEP SLSSPWFTSQ SALLSSRRKP LLVGVFQNQS 600
LSDILSAVDE IGLDLVQLHG DEPQAWAKFI PVPVVKVFRV SPEGIVRGGE 650
IRRPGLNQAI LLDAGGASGG GGEGKAFPWE HAKRLIQSGE VGSEGHVPLP 700
VILAGGLTPE NVGQAIEQAG EGVWCVDVSS GVEGEGGKVK EKVEAFVKAV 750
RG 752
Length:752
Mass (Da):80,194
Last modified:June 21, 2005 - v2
Checksum:iFE31FEF7BFC5FC91
GO

Sequence cautioni

The sequence AAA51445.1 differs from that shown. Reason: Frameshift at positions 297, 302, 307, 310 and 716.
The sequence AAA51445.1 differs from that shown. Reason: Erroneous termination at position 324. Translated as Glu.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti295 – 30915KGDIA…PQQAL → FQGRNAPTLLLLRST1 Publication
Add
BLAST
Sequence conflicti504 – 5041P → S in AAA51445. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003828 Genomic DNA. Translation: AFR97220.1.
M74901 Genomic DNA. Translation: AAA51445.1. Sequence problems.
PIRiJN0451.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP003828 Genomic DNA. Translation: AFR97220.1 .
M74901 Genomic DNA. Translation: AAA51445.1 . Sequence problems.
PIRi JN0451.

3D structure databases

ProteinModelPortali P27710.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Phylogenomic databases

OrthoDBi EOG78WM1Q.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00040 .
UPA00035 ; UER00042 .
UPA00035 ; UER00043 .

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
3.40.50.880. 1 hit.
HAMAPi MF_00135. PRAI.
InterProi IPR013785. Aldolase_TIM.
IPR016302. Anthranilate_synth_II.
IPR029062. Class_I_gatase-like.
IPR017926. GATASE.
IPR013798. Indole-3-glycerol_P_synth.
IPR001468. Indole-3-GlycerolPSynthase_CS.
IPR001240. PRAI_dom.
IPR011060. RibuloseP-bd_barrel.
IPR006221. TrpG/PapA_dom.
[Graphical view ]
Pfami PF00117. GATase. 1 hit.
PF00218. IGPS. 1 hit.
PF00697. PRAI. 1 hit.
[Graphical view ]
PIRSFi PIRSF001382. TrpG-trpC-trpF. 1 hit.
SUPFAMi SSF51366. SSF51366. 3 hits.
SSF52317. SSF52317. 1 hit.
TIGRFAMsi TIGR00566. trpG_papA. 1 hit.
PROSITEi PS51273. GATASE_TYPE_1. 1 hit.
PS00614. IGPS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the genome and transcriptome of Cryptococcus neoformans var. grubii reveals complex RNA expression and microevolution leading to virulence attenuation."
    Janbon G., Ormerod K.L., Paulet D., Byrnes E.J. III, Yadav V., Chatterjee G., Mullapudi N., Hon C.-C., Billmyre R.B., Brunel F., Bahn Y.-S., Chen W., Chen Y., Chow E.W.L., Coppee J.-Y., Floyd-Averette A., Gaillardin C., Gerik K.J.
    , Goldberg J., Gonzalez-Hilarion S., Gujja S., Hamlin J.L., Hsueh Y.-P., Ianiri G., Jones S., Kodira C.D., Kozubowski L., Lam W., Marra M., Mesner L.D., Mieczkowski P.A., Moyrand F., Nielsen K., Proux C., Rossignol T., Schein J.E., Sun S., Wollschlaeger C., Wood I.A., Zeng Q., Neuveglise C., Newlon C.S., Perfect J.R., Lodge J.K., Idnurm A., Stajich J.E., Kronstad J.W., Sanyal K., Heitman J., Fraser J.A., Cuomo C.A., Dietrich F.S.
    PLoS Genet. 10:E1004261-E1004261(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: H99 / ATCC 208821 / CBS 10515 / FGSC 9487.
  2. "Cloning the Cryptococcus neoformans TRP1 gene by complementation in Saccharomyces cerevisiae."
    Perfect J.R., Rude T.H., Penning L.M., Johnston S.A.
    Gene 122:213-217(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 294-752.

Entry informationi

Entry nameiTRPG_CRYNH
AccessioniPrimary (citable) accession number: P27710
Secondary accession number(s): J9VZN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 21, 2005
Last modified: June 11, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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