Regulatory protein MIG1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P27705 (MIG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 128. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Regulatory protein MIG1

Alternative name(s):
Regulatory protein CAT4

Gene names

Name:	MIG1
Synonyms:	CAT4, SSN1
Ordered Locus Names:	YGL035C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in glucose repression of the SUC, GAL and MAL genes as well as of the CAT8 gene. Binds to two sites in the upstream region of SUC2.
Subcellular location	Nucleus.
Miscellaneous	Present with 830 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the creA/MIG C2H2-type zinc-finger protein family. Contains 2 C2H2-type zinc fingers.
Sequence caution	The sequence CAA39084.1 differs from that shown. Reason: Frameshift at position 381.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Transcription Transcription regulation
Cellular component	Nucleus
Domain	Repeat Zinc-finger
Ligand	DNA-binding Metal-binding Zinc
Molecular function	Repressor
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of transcription from RNA polymerase II promoter by glucose Inferred from direct assay PubMed 7724528. Source: SGD positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Inferred from genetic interaction PubMed 22904036. Source: SGD positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 9832517. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 11914276 PubMed 17237508 PubMed 9285828. Source: SGD nuclear envelope lumen Inferred from direct assay PubMed 17237508. Source: SGD
Molecular_function	RNA polymerase II core promoter proximal region sequence-specific DNA binding Inferred from direct assay PubMed 12239282 PubMed 8114729 PubMed 9832517. Source: SGD RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Inferred from direct assay PubMed 7724528 PubMed 9832517. Source: SGD zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSP82	P02829	3	EBI-10913,EBI-8659

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 504

504

Regulatory protein MIG1

PRO_0000046881

Regions

Zinc finger

38 – 60

C2H2-type 1

Zinc finger

66 – 90

C2H2-type 2

Compositional bias

253 – 260

Gln-rich

Compositional bias

411 – 444

Asn/Gln-rich

Amino acid modifications

Modified residue

179

Phosphoserine Ref.9

Modified residue

218

Phosphoserine Ref.9

Modified residue

278

Phosphoserine Ref.9

Modified residue

280

Phosphothreonine Ref.9

Modified residue

302

Phosphoserine Ref.9

Modified residue

310

Phosphoserine Ref.9

Modified residue

311

Phosphoserine Ref.7 Ref.8 Ref.9

Modified residue

314

Phosphoserine Ref.8 Ref.9

Modified residue

350

Phosphoserine Ref.9

Modified residue

371

Phosphothreonine Ref.9

Modified residue

377

Phosphoserine Ref.9

Modified residue

379

Phosphoserine Ref.9

Modified residue

381

Phosphoserine Ref.9

Modified residue

402

Phosphoserine Ref.9

Modified residue

451

Phosphoserine Ref.9

Modified residue

455

Phosphothreonine Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P27705 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 5D3B883D8FA15B7F
Show »

FASTA

504

55,532

        10         20         30         40         50         60 
MQSPYPMTQV SNVDDGSLLK ESKSKSKVAA KSEAPRPHAC PICHRAFHRL EHQTRHMRIH 

        70         80         90        100        110        120 
TGEKPHACDF PGCVKRFSRS DELTRHRRIH TNSHPRGKRG RKKKVVGSPI NSASSSATSI 

       130        140        150        160        170        180 
PDLNTANFSP PLPQQHLSPL IPIAIAPKEN SSRSSTRKGR KTKFEIGESG GNDPYMVSSP 

       190        200        210        220        230        240 
KTMAKIPVSV KPPPSLALNN MNYQTSSAST ALSSLSNSHS GSRLKLNALS SLQMMTPIAS 

       250        260        270        280        290        300 
SAPRTVFIDG PEQKQLQQQQ NSLSPRYSNT VILPRPRSLT DFQGLNNANP NNNGSLRAQT 

       310        320        330        340        350        360 
QSSVQLKRPS SVLSLNDLLV GQRNTNESDS DFTTGGEDEE DGLKDPSNSS IDNLEQDYLQ 

       370        380        390        400        410        420 
EQSRKKSKTS TPTTMLSRST SGTNLHTLGY VMNQNHLHFS SSSPDFQKEL NNRLLNVQQQ 

       430        440        450        460        470        480 
QQEQHTLLQS QNTSNQSQNQ NQNQMMASSS SLSTTPLLLS PRVNMINTAI STQQTPISQS 

       490        500 
DSQVQELETL PPIRSLPLPF PHMD

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins." Nehlin J.O., Ronne H. EMBO J. 9:2891-2898(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 208353 / W303-1A.
[2]	Huse K., Hohmannn S., Valentin E., Zimmermann F.K. Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204510 / AB320.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. Kleine K. Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311 AND SER-314, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-179; SER-218; SER-278; THR-280; SER-302; SER-310; SER-311; SER-314; SER-350; THR-371; SER-377; SER-379; SER-381; SER-402; SER-451 AND THR-455, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X55734 Genomic DNA. Translation: CAA39266.1.
X55442 Genomic DNA. Translation: CAA39084.1. Frameshift.
Z72557 Genomic DNA. Translation: CAA96736.1.
AY693159 Genomic DNA. Translation: AAT93178.1.
BK006941 Genomic DNA. Translation: DAA08065.1.

PIR

S17248.

RefSeq

NP_011480.1. NM_001180900.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1T5W	X-ray	2.40	C/F	455-462	[»]
1T5X	X-ray	2.50	C	455-462	[»]

ProteinModelPortal

P27705.

SMR

P27705. Positions 36-97.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-665N.

IntAct

P27705. 4 interactions.

MINT

MINT-400332.

STRING

4932.YGL035C.

Proteomic databases

PaxDb

P27705.

PeptideAtlas

P27705.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YGL035C; YGL035C; YGL035C.

GeneID

852848.

KEGG

sce:YGL035C.

Organism-specific databases

CYGD

YGL035c.

SGD

S000003003. MIG1.

Phylogenomic databases

eggNOG

COG5048.

GeneTree

ENSGT00700000104821.

HOGENOM

HOG000113589.

K09467.

OMA

EHQTRHM.

OrthoDB

EOG41G6D6.

Gene expression databases

Genevestigator

P27705.

GermOnline

YGL035C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.160.60. 2 hits.

InterPro

IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]

SMART

SM00355. ZnF_C2H2. 2 hits.
[Graphical view]

PROSITE

PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P27705.

NextBio

972437.

Entry information

Entry name

MIG1_YEAST

Accession

Primary (citable) accession number: P27705
Secondary accession number(s): D6VUA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents