Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P27705

- MIG1_YEAST

UniProt

P27705 - MIG1_YEAST

Protein

Regulatory protein MIG1

Gene

MIG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in glucose repression of the SUC, GAL and MAL genes as well as of the CAT8 gene. Binds to two sites in the upstream region of SUC2.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri38 – 6023C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri66 – 9025C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
    4. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD

    GO - Biological processi

    1. carbohydrate metabolic process Source: UniProtKB-KW
    2. negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
    3. positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Source: SGD
    4. positive regulation of transcription from RNA polymerase II promoter Source: SGD

    Keywords - Molecular functioni

    Repressor

    Keywords - Biological processi

    Carbohydrate metabolism, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Enzyme and pathway databases

    BioCyciYEAST:G3O-30550-MONOMER.
    ReactomeiREACT_189033. Transcriptional regulation of white adipocyte differentiation.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Regulatory protein MIG1
    Alternative name(s):
    Regulatory protein CAT4
    Gene namesi
    Name:MIG1
    Synonyms:CAT4, SSN1
    Ordered Locus Names:YGL035C
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome VII

    Organism-specific databases

    CYGDiYGL035c.
    SGDiS000003003. MIG1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: SGD
    2. nuclear envelope lumen Source: SGD
    3. nucleus Source: SGD

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 504504Regulatory protein MIG1PRO_0000046881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei278 – 2781Phosphoserine1 Publication
    Modified residuei302 – 3021Phosphoserine1 Publication
    Modified residuei310 – 3101Phosphoserine1 Publication
    Modified residuei311 – 3111Phosphoserine1 Publication
    Modified residuei314 – 3141Phosphoserine1 Publication
    Modified residuei377 – 3771Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27705.
    PaxDbiP27705.
    PeptideAtlasiP27705.

    Expressioni

    Gene expression databases

    GenevestigatoriP27705.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSP82P028293EBI-10913,EBI-8659

    Protein-protein interaction databases

    BioGridi33212. 152 interactions.
    DIPiDIP-665N.
    IntActiP27705. 7 interactions.
    MINTiMINT-400332.
    STRINGi4932.YGL035C.

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1T5WX-ray2.40C/F455-462[»]
    1T5XX-ray2.50C455-462[»]
    ProteinModelPortaliP27705.
    SMRiP27705. Positions 36-97.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27705.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi253 – 2608Gln-rich
    Compositional biasi411 – 44434Asn/Gln-richAdd
    BLAST

    Sequence similaritiesi

    Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri38 – 6023C2H2-type 1PROSITE-ProRule annotationAdd
    BLAST
    Zinc fingeri66 – 9025C2H2-type 2PROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiCOG5048.
    GeneTreeiENSGT00550000074455.
    HOGENOMiHOG000113589.
    KOiK09467.
    OMAiPICHRAF.
    OrthoDBiEOG7K0ZMS.

    Family and domain databases

    Gene3Di3.30.160.60. 2 hits.
    InterProiIPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 2 hits.
    [Graphical view]
    PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27705-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQSPYPMTQV SNVDDGSLLK ESKSKSKVAA KSEAPRPHAC PICHRAFHRL    50
    EHQTRHMRIH TGEKPHACDF PGCVKRFSRS DELTRHRRIH TNSHPRGKRG 100
    RKKKVVGSPI NSASSSATSI PDLNTANFSP PLPQQHLSPL IPIAIAPKEN 150
    SSRSSTRKGR KTKFEIGESG GNDPYMVSSP KTMAKIPVSV KPPPSLALNN 200
    MNYQTSSAST ALSSLSNSHS GSRLKLNALS SLQMMTPIAS SAPRTVFIDG 250
    PEQKQLQQQQ NSLSPRYSNT VILPRPRSLT DFQGLNNANP NNNGSLRAQT 300
    QSSVQLKRPS SVLSLNDLLV GQRNTNESDS DFTTGGEDEE DGLKDPSNSS 350
    IDNLEQDYLQ EQSRKKSKTS TPTTMLSRST SGTNLHTLGY VMNQNHLHFS 400
    SSSPDFQKEL NNRLLNVQQQ QQEQHTLLQS QNTSNQSQNQ NQNQMMASSS 450
    SLSTTPLLLS PRVNMINTAI STQQTPISQS DSQVQELETL PPIRSLPLPF 500
    PHMD 504
    Length:504
    Mass (Da):55,532
    Last modified:August 1, 1992 - v1
    Checksum:i5D3B883D8FA15B7F
    GO

    Sequence cautioni

    The sequence CAA39084.1 differs from that shown. Reason: Frameshift at position 381.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55734 Genomic DNA. Translation: CAA39266.1.
    X55442 Genomic DNA. Translation: CAA39084.1. Frameshift.
    Z72557 Genomic DNA. Translation: CAA96736.1.
    AY693159 Genomic DNA. Translation: AAT93178.1.
    BK006941 Genomic DNA. Translation: DAA08065.1.
    PIRiS17248.
    RefSeqiNP_011480.1. NM_001180900.1.

    Genome annotation databases

    EnsemblFungiiYGL035C; YGL035C; YGL035C.
    GeneIDi852848.
    KEGGisce:YGL035C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X55734 Genomic DNA. Translation: CAA39266.1 .
    X55442 Genomic DNA. Translation: CAA39084.1 . Frameshift.
    Z72557 Genomic DNA. Translation: CAA96736.1 .
    AY693159 Genomic DNA. Translation: AAT93178.1 .
    BK006941 Genomic DNA. Translation: DAA08065.1 .
    PIRi S17248.
    RefSeqi NP_011480.1. NM_001180900.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1T5W X-ray 2.40 C/F 455-462 [» ]
    1T5X X-ray 2.50 C 455-462 [» ]
    ProteinModelPortali P27705.
    SMRi P27705. Positions 36-97.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33212. 152 interactions.
    DIPi DIP-665N.
    IntActi P27705. 7 interactions.
    MINTi MINT-400332.
    STRINGi 4932.YGL035C.

    Proteomic databases

    MaxQBi P27705.
    PaxDbi P27705.
    PeptideAtlasi P27705.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YGL035C ; YGL035C ; YGL035C .
    GeneIDi 852848.
    KEGGi sce:YGL035C.

    Organism-specific databases

    CYGDi YGL035c.
    SGDi S000003003. MIG1.

    Phylogenomic databases

    eggNOGi COG5048.
    GeneTreei ENSGT00550000074455.
    HOGENOMi HOG000113589.
    KOi K09467.
    OMAi PICHRAF.
    OrthoDBi EOG7K0ZMS.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-30550-MONOMER.
    Reactomei REACT_189033. Transcriptional regulation of white adipocyte differentiation.

    Miscellaneous databases

    EvolutionaryTracei P27705.
    NextBioi 972437.

    Gene expression databases

    Genevestigatori P27705.

    Family and domain databases

    Gene3Di 3.30.160.60. 2 hits.
    InterProi IPR007087. Znf_C2H2.
    IPR015880. Znf_C2H2-like.
    IPR013087. Znf_C2H2/integrase_DNA-bd.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 2 hits.
    [Graphical view ]
    PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
    PS50157. ZINC_FINGER_C2H2_2. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins."
      Nehlin J.O., Ronne H.
      EMBO J. 9:2891-2898(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 208353 / W303-1A.
    2. Huse K., Hohmannn S., Valentin E., Zimmermann F.K.
      Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204510 / AB320.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
      Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
      , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
      Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278; SER-310; SER-311 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiMIG1_YEAST
    AccessioniPrimary (citable) accession number: P27705
    Secondary accession number(s): D6VUA4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 141 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 830 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome VII
      Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

    External Data

    Dasty 3