Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P27705

- MIG1_YEAST

UniProt

P27705 - MIG1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Regulatory protein MIG1

Gene

MIG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Involved in glucose repression of the SUC, GAL and MAL genes as well as of the CAT8 gene. Binds to two sites in the upstream region of SUC2.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri66 – 9025C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: SGD
  3. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription Source: SGD

GO - Biological processi

  1. carbohydrate metabolic process Source: UniProtKB-KW
  2. negative regulation of transcription from RNA polymerase II promoter Source: SGD
  3. negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  4. positive regulation of filamentous growth of a population of unicellular organisms in response to starvation Source: SGD
  5. positive regulation of transcription from RNA polymerase II promoter Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Carbohydrate metabolism, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-30550-MONOMER.
ReactomeiREACT_189033. Transcriptional regulation of white adipocyte differentiation.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulatory protein MIG1
Alternative name(s):
Regulatory protein CAT4
Gene namesi
Name:MIG1
Synonyms:CAT4, SSN1
Ordered Locus Names:YGL035C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome VII

Organism-specific databases

CYGDiYGL035c.
SGDiS000003003. MIG1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: SGD
  2. nuclear envelope lumen Source: SGD
  3. nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 504504Regulatory protein MIG1PRO_0000046881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei278 – 2781Phosphoserine1 Publication
Modified residuei302 – 3021Phosphoserine1 Publication
Modified residuei310 – 3101Phosphoserine1 Publication
Modified residuei311 – 3111Phosphoserine1 Publication
Modified residuei314 – 3141Phosphoserine1 Publication
Modified residuei377 – 3771Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27705.
PaxDbiP27705.
PeptideAtlasiP27705.

Expressioni

Gene expression databases

GenevestigatoriP27705.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP82P028293EBI-10913,EBI-8659

Protein-protein interaction databases

BioGridi33212. 152 interactions.
DIPiDIP-665N.
IntActiP27705. 7 interactions.
MINTiMINT-400332.
STRINGi4932.YGL035C.

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1T5WX-ray2.40C/F455-462[»]
1T5XX-ray2.50C455-462[»]
ProteinModelPortaliP27705.
SMRiP27705. Positions 36-97.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27705.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi253 – 2608Gln-rich
Compositional biasi411 – 44434Asn/Gln-richAdd
BLAST

Sequence similaritiesi

Contains 2 C2H2-type zinc fingers.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri38 – 6023C2H2-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri66 – 9025C2H2-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiCOG5048.
GeneTreeiENSGT00550000074455.
HOGENOMiHOG000113589.
InParanoidiP27705.
KOiK09467.
OMAiPICHRAF.
OrthoDBiEOG7K0ZMS.

Family and domain databases

Gene3Di3.30.160.60. 2 hits.
InterProiIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEiPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27705-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQSPYPMTQV SNVDDGSLLK ESKSKSKVAA KSEAPRPHAC PICHRAFHRL
60 70 80 90 100
EHQTRHMRIH TGEKPHACDF PGCVKRFSRS DELTRHRRIH TNSHPRGKRG
110 120 130 140 150
RKKKVVGSPI NSASSSATSI PDLNTANFSP PLPQQHLSPL IPIAIAPKEN
160 170 180 190 200
SSRSSTRKGR KTKFEIGESG GNDPYMVSSP KTMAKIPVSV KPPPSLALNN
210 220 230 240 250
MNYQTSSAST ALSSLSNSHS GSRLKLNALS SLQMMTPIAS SAPRTVFIDG
260 270 280 290 300
PEQKQLQQQQ NSLSPRYSNT VILPRPRSLT DFQGLNNANP NNNGSLRAQT
310 320 330 340 350
QSSVQLKRPS SVLSLNDLLV GQRNTNESDS DFTTGGEDEE DGLKDPSNSS
360 370 380 390 400
IDNLEQDYLQ EQSRKKSKTS TPTTMLSRST SGTNLHTLGY VMNQNHLHFS
410 420 430 440 450
SSSPDFQKEL NNRLLNVQQQ QQEQHTLLQS QNTSNQSQNQ NQNQMMASSS
460 470 480 490 500
SLSTTPLLLS PRVNMINTAI STQQTPISQS DSQVQELETL PPIRSLPLPF

PHMD
Length:504
Mass (Da):55,532
Last modified:August 1, 1992 - v1
Checksum:i5D3B883D8FA15B7F
GO

Sequence cautioni

The sequence CAA39084.1 differs from that shown. Reason: Frameshift at position 381.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55734 Genomic DNA. Translation: CAA39266.1.
X55442 Genomic DNA. Translation: CAA39084.1. Frameshift.
Z72557 Genomic DNA. Translation: CAA96736.1.
AY693159 Genomic DNA. Translation: AAT93178.1.
BK006941 Genomic DNA. Translation: DAA08065.1.
PIRiS17248.
RefSeqiNP_011480.1. NM_001180900.1.

Genome annotation databases

EnsemblFungiiYGL035C; YGL035C; YGL035C.
GeneIDi852848.
KEGGisce:YGL035C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X55734 Genomic DNA. Translation: CAA39266.1 .
X55442 Genomic DNA. Translation: CAA39084.1 . Frameshift.
Z72557 Genomic DNA. Translation: CAA96736.1 .
AY693159 Genomic DNA. Translation: AAT93178.1 .
BK006941 Genomic DNA. Translation: DAA08065.1 .
PIRi S17248.
RefSeqi NP_011480.1. NM_001180900.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1T5W X-ray 2.40 C/F 455-462 [» ]
1T5X X-ray 2.50 C 455-462 [» ]
ProteinModelPortali P27705.
SMRi P27705. Positions 36-97.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33212. 152 interactions.
DIPi DIP-665N.
IntActi P27705. 7 interactions.
MINTi MINT-400332.
STRINGi 4932.YGL035C.

Proteomic databases

MaxQBi P27705.
PaxDbi P27705.
PeptideAtlasi P27705.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YGL035C ; YGL035C ; YGL035C .
GeneIDi 852848.
KEGGi sce:YGL035C.

Organism-specific databases

CYGDi YGL035c.
SGDi S000003003. MIG1.

Phylogenomic databases

eggNOGi COG5048.
GeneTreei ENSGT00550000074455.
HOGENOMi HOG000113589.
InParanoidi P27705.
KOi K09467.
OMAi PICHRAF.
OrthoDBi EOG7K0ZMS.

Enzyme and pathway databases

BioCyci YEAST:G3O-30550-MONOMER.
Reactomei REACT_189033. Transcriptional regulation of white adipocyte differentiation.

Miscellaneous databases

EvolutionaryTracei P27705.
NextBioi 972437.

Gene expression databases

Genevestigatori P27705.

Family and domain databases

Gene3Di 3.30.160.60. 2 hits.
InterProi IPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 2 hits.
[Graphical view ]
PROSITEi PS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins."
    Nehlin J.O., Ronne H.
    EMBO J. 9:2891-2898(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 208353 / W303-1A.
  2. Huse K., Hohmannn S., Valentin E., Zimmermann F.K.
    Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204510 / AB320.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
    Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E.
    , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
    Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278; SER-310; SER-311 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMIG1_YEAST
AccessioniPrimary (citable) accession number: P27705
Secondary accession number(s): D6VUA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

External Data

Dasty 3