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P27705 (MIG1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulatory protein MIG1
Alternative name(s):
Regulatory protein CAT4
Gene names
Name:MIG1
Synonyms:CAT4, SSN1
Ordered Locus Names:YGL035C
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length504 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in glucose repression of the SUC, GAL and MAL genes as well as of the CAT8 gene. Binds to two sites in the upstream region of SUC2.

Subcellular location

Nucleus.

Miscellaneous

Present with 830 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the creA/MIG C2H2-type zinc-finger protein family.

Contains 2 C2H2-type zinc fingers.

Sequence caution

The sequence CAA39084.1 differs from that shown. Reason: Frameshift at position 381.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainRepeat
Zinc-finger
   LigandDNA-binding
Metal-binding
Zinc
   Molecular functionRepressor
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

negative regulation of transcription from RNA polymerase II promoter by glucose

Inferred from direct assay PubMed 7724528. Source: SGD

positive regulation of filamentous growth of a population of unicellular organisms in response to starvation

Inferred from genetic interaction PubMed 22904036. Source: SGD

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype PubMed 9832517. Source: SGD

   Cellular_componentcytoplasm

Inferred from direct assay PubMed 17237508PubMed 9285828. Source: SGD

nuclear envelope lumen

Inferred from direct assay PubMed 17237508. Source: SGD

nucleus

Inferred from direct assay PubMed 17237508PubMed 9285828. Source: SGD

   Molecular_functionRNA polymerase II core promoter proximal region sequence-specific DNA binding

Inferred from direct assay PubMed 12239282PubMed 8114729PubMed 9832517. Source: SGD

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in negative regulation of transcription

Inferred from direct assay PubMed 7724528PubMed 9832517. Source: SGD

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP82P028293EBI-10913,EBI-8659

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 504504Regulatory protein MIG1
PRO_0000046881

Regions

Zinc finger38 – 6023C2H2-type 1
Zinc finger66 – 9025C2H2-type 2
Compositional bias253 – 2608Gln-rich
Compositional bias411 – 44434Asn/Gln-rich

Amino acid modifications

Modified residue2781Phosphoserine Ref.10
Modified residue3021Phosphoserine Ref.9
Modified residue3101Phosphoserine Ref.10
Modified residue3111Phosphoserine Ref.10
Modified residue3141Phosphoserine Ref.10
Modified residue3771Phosphoserine Ref.9

Sequences

Sequence LengthMass (Da)Tools
P27705 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 5D3B883D8FA15B7F

FASTA50455,532
        10         20         30         40         50         60 
MQSPYPMTQV SNVDDGSLLK ESKSKSKVAA KSEAPRPHAC PICHRAFHRL EHQTRHMRIH 

        70         80         90        100        110        120 
TGEKPHACDF PGCVKRFSRS DELTRHRRIH TNSHPRGKRG RKKKVVGSPI NSASSSATSI 

       130        140        150        160        170        180 
PDLNTANFSP PLPQQHLSPL IPIAIAPKEN SSRSSTRKGR KTKFEIGESG GNDPYMVSSP 

       190        200        210        220        230        240 
KTMAKIPVSV KPPPSLALNN MNYQTSSAST ALSSLSNSHS GSRLKLNALS SLQMMTPIAS 

       250        260        270        280        290        300 
SAPRTVFIDG PEQKQLQQQQ NSLSPRYSNT VILPRPRSLT DFQGLNNANP NNNGSLRAQT 

       310        320        330        340        350        360 
QSSVQLKRPS SVLSLNDLLV GQRNTNESDS DFTTGGEDEE DGLKDPSNSS IDNLEQDYLQ 

       370        380        390        400        410        420 
EQSRKKSKTS TPTTMLSRST SGTNLHTLGY VMNQNHLHFS SSSPDFQKEL NNRLLNVQQQ 

       430        440        450        460        470        480 
QQEQHTLLQS QNTSNQSQNQ NQNQMMASSS SLSTTPLLLS PRVNMINTAI STQQTPISQS 

       490        500 
DSQVQELETL PPIRSLPLPF PHMD 

« Hide

References

« Hide 'large scale' references
[1]"Yeast MIG1 repressor is related to the mammalian early growth response and Wilms' tumour finger proteins."
Nehlin J.O., Ronne H.
EMBO J. 9:2891-2898(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 208353 / W303-1A.
[2]Huse K., Hohmannn S., Valentin E., Zimmermann F.K.
Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204510 / AB320.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII."
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. expand/collapse author list , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.
Nature 387:81-84(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-302 AND SER-377, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-278; SER-310; SER-311 AND SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X55734 Genomic DNA. Translation: CAA39266.1.
X55442 Genomic DNA. Translation: CAA39084.1. Frameshift.
Z72557 Genomic DNA. Translation: CAA96736.1.
AY693159 Genomic DNA. Translation: AAT93178.1.
BK006941 Genomic DNA. Translation: DAA08065.1.
PIRS17248.
RefSeqNP_011480.1. NM_001180900.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1T5WX-ray2.40C/F455-462[»]
1T5XX-ray2.50C455-462[»]
ProteinModelPortalP27705.
SMRP27705. Positions 36-107.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33212. 150 interactions.
DIPDIP-665N.
IntActP27705. 7 interactions.
MINTMINT-400332.
STRING4932.YGL035C.

Proteomic databases

PaxDbP27705.
PeptideAtlasP27705.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYGL035C; YGL035C; YGL035C.
GeneID852848.
KEGGsce:YGL035C.

Organism-specific databases

CYGDYGL035c.
SGDS000003003. MIG1.

Phylogenomic databases

eggNOGCOG5048.
GeneTreeENSGT00550000074455.
HOGENOMHOG000113589.
KOK09467.
OMAEHQTRHM.
OrthoDBEOG7K0ZMS.

Enzyme and pathway databases

BioCycYEAST:G3O-30550-MONOMER.

Gene expression databases

GenevestigatorP27705.

Family and domain databases

Gene3D3.30.160.60. 2 hits.
InterProIPR007087. Znf_C2H2.
IPR015880. Znf_C2H2-like.
IPR013087. Znf_C2H2/integrase_DNA-bd.
[Graphical view]
SMARTSM00355. ZnF_C2H2. 2 hits.
[Graphical view]
PROSITEPS00028. ZINC_FINGER_C2H2_1. 2 hits.
PS50157. ZINC_FINGER_C2H2_2. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP27705.
NextBio972437.

Entry information

Entry nameMIG1_YEAST
AccessionPrimary (citable) accession number: P27705
Secondary accession number(s): D6VUA4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome VII

Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references