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P27704

- MK06_RAT

UniProt

P27704 - MK06_RAT

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Protein
Mitogen-activated protein kinase 6
Gene
Mapk6, Erk3, Prkm6
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle By similarity.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Magnesium By similarity.

Enzyme regulationi

Activated by phosphorylation at Ser-189 By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei49 – 491ATP By similarity
Active sitei152 – 1521Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi26 – 349ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB

GO - Biological processi

  1. cell cycle Source: UniProtKB-KW
  2. protein phosphorylation Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Cell cycle

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase 6 (EC:2.7.11.24)
Short name:
MAP kinase 6
Short name:
MAPK 6
Alternative name(s):
Extracellular signal-regulated kinase 3
Short name:
ERK-3
p55-MAPK
Gene namesi
Name:Mapk6
Synonyms:Erk3, Prkm6
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 8

Organism-specific databases

RGDi62087. Mapk6.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity
Note: Translocates to the cytoplasm following interaction with MAPKAPK5 By similarity.

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Mitogen-activated protein kinase 6
PRO_0000186259Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Cross-linki1 – 1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin) By similarity
Modified residuei189 – 1891Phosphoserine; by PAK1, PAK2 and PAK3 By similarity

Post-translational modificationi

Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites By similarity.
Ubiquitination at Met-1 leads to degradation by the proteasome pathway By similarity.

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP27704.

PTM databases

PhosphoSiteiP27704.

Expressioni

Tissue specificityi

Highest levels within the nervous system, expressed in different tissues, mostly in skeletal muscle.

Developmental stagei

Expressed at highest levels early in development.

Gene expression databases

GenevestigatoriP27704.

Interactioni

Subunit structurei

Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 By similarity. Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4 By similarity.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000013053.

Structurei

3D structure databases

ProteinModelPortaliP27704.
SMRiP27704. Positions 13-321.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 316297Protein kinase
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi189 – 1913SEG motif
Motifi332 – 3376FRIEDE motif

Domaini

In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases By similarity.

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074298.
HOGENOMiHOG000233020.
HOVERGENiHBG104376.
InParanoidiP27704.
KOiK06855.
OMAiEHDWPIH.
OrthoDBiEOG780RKS.
PhylomeDBiP27704.
TreeFamiTF105098.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR008350. MAPK_ERK3/4.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01771. ERK3ERK4MAPK.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27704-1 [UniParc]FASTAAdd to Basket

« Hide

MAEKFESLMN IHGFDLGSRY MDLKPLGCGG NGLVFSAVDN DCDKRVAIKK    50
IVLTDPQSVK HALREIKIIR RLDHDNIVKV FEILGPSGSQ LTDDVGSLTE 100
LNSVYIVQEY METDLANVLE QGPLLEEHAR LFMYQLLRGL KYIHSANVLH 150
RDLKPANLFI NTEDLVLKIG DFGLARIMDP HYSHKGHLSE GLVTKWYRSP 200
RLLLSPNNYT KAIDMWAAGC IFAEMLTGKT LFAGAHELEQ MQLILESIPV 250
VHEEDRQELL SVIPVYIRND MTEPHKPLTQ LLPGISREAL DFLEQILTFS 300
PMDRLTAEEA LSHPYMSIYS FPTDEPISSH PFHIEDEVDD ILLMDETHSH 350
IYNWERYHDC QFSEHDWPIH NNFDIDEVQL DPRALSDVTD EEEVQVDPRK 400
YLDGDREKYL EDPAFDTSYS AEPCWQYPDH HENKYCDLEC SHTCNYKTRS 450
PSYLDNLVWR ESEVNHYYEP KLIIDLSNWK EQSKDKSDKR GKSKCERNGL 500
VKAQIALEEA SQQLAERERG QGFDFDAFIA GTVQLSAQRE SADVVDKLND 550
LNSSVSQLEM KSLISKSVSR EKQEKGRANL AQLGALYQPS WESQFVSGGE 600
ECFLISQFCC EVRKDEHVEK ENTYTSYLDK FFSRKEDSEM LETEPVEEGK 650
RGERGREAGL LSSGGEFLLS RQLESIGTPQ FHSPGGSPLK SIQATLTPSA 700
MKSSPQIPHK TYSNILKHLN 720
Length:720
Mass (Da):82,275
Last modified:November 1, 1997 - v2
Checksum:iB200B215574AB84D
GO

Sequence cautioni

The sequence AAA41125.1 differs from that shown. Reason: Frameshift at position 503.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64301 mRNA. Translation: AAA41125.1. Frameshift.
BC109380 mRNA. Translation: AAI09381.1.
PIRiB40033.
RefSeqiNP_113810.2. NM_031622.2.
XP_006243471.1. XM_006243409.1.
UniGeneiRn.88457.

Genome annotation databases

EnsembliENSRNOT00000013053; ENSRNOP00000013053; ENSRNOG00000009381.
GeneIDi58840.
KEGGirno:58840.
UCSCiRGD:62087. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M64301 mRNA. Translation: AAA41125.1 . Frameshift.
BC109380 mRNA. Translation: AAI09381.1 .
PIRi B40033.
RefSeqi NP_113810.2. NM_031622.2.
XP_006243471.1. XM_006243409.1.
UniGenei Rn.88457.

3D structure databases

ProteinModelPortali P27704.
SMRi P27704. Positions 13-321.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10116.ENSRNOP00000013053.

PTM databases

PhosphoSitei P27704.

Proteomic databases

PaxDbi P27704.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000013053 ; ENSRNOP00000013053 ; ENSRNOG00000009381 .
GeneIDi 58840.
KEGGi rno:58840.
UCSCi RGD:62087. rat.

Organism-specific databases

CTDi 5597.
RGDi 62087. Mapk6.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00550000074298.
HOGENOMi HOG000233020.
HOVERGENi HBG104376.
InParanoidi P27704.
KOi K06855.
OMAi EHDWPIH.
OrthoDBi EOG780RKS.
PhylomeDBi P27704.
TreeFami TF105098.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 5301.

Miscellaneous databases

NextBioi 611410.
PROi P27704.

Gene expression databases

Genevestigatori P27704.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR008350. MAPK_ERK3/4.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01771. ERK3ERK4MAPK.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF."
    Boulton T.G., Nye S.H., Robbins D.J., Ip N.Y., Radziejewska E., Morgenbesser S.D., DePinho R.A., Panayotatos N., Cobb M.H., Yancopoulos G.D.
    Cell 65:663-675(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain and Liver.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Placenta.

Entry informationi

Entry nameiMK06_RAT
AccessioniPrimary (citable) accession number: P27704
Secondary accession number(s): Q32LY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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