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P27704

- MK06_RAT

UniProt

P27704 - MK06_RAT

Protein

Mitogen-activated protein kinase 6

Gene

Mapk6

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by phosphorylation at Ser-189.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei49 – 491ATPPROSITE-ProRule annotation
    Active sitei152 – 1521Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi26 – 349ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. protein phosphorylation Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase 6 (EC:2.7.11.24)
    Short name:
    MAP kinase 6
    Short name:
    MAPK 6
    Alternative name(s):
    Extracellular signal-regulated kinase 3
    Short name:
    ERK-3
    p55-MAPK
    Gene namesi
    Name:Mapk6
    Synonyms:Erk3, Prkm6
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 8

    Organism-specific databases

    RGDi62087. Mapk6.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity
    Note: Translocates to the cytoplasm following interaction with MAPKAPK5.By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Mitogen-activated protein kinase 6PRO_0000186259Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Cross-linki1 – 1Peptide (Met-Gly) (interchain with G-Cter in ubiquitin)By similarity
    Modified residuei189 – 1891Phosphoserine; by PAK1, PAK2 and PAK3By similarity

    Post-translational modificationi

    Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites By similarity.By similarity
    Ubiquitination at Met-1 leads to degradation by the proteasome pathway.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Proteomic databases

    PaxDbiP27704.

    PTM databases

    PhosphoSiteiP27704.

    Expressioni

    Tissue specificityi

    Highest levels within the nervous system, expressed in different tissues, mostly in skeletal muscle.

    Developmental stagei

    Expressed at highest levels early in development.

    Gene expression databases

    GenevestigatoriP27704.

    Interactioni

    Subunit structurei

    Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 By similarity. Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4 By similarity.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000013053.

    Structurei

    3D structure databases

    ProteinModelPortaliP27704.
    SMRiP27704. Positions 13-321.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini20 – 316297Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi189 – 1913SEG motif
    Motifi332 – 3376FRIEDE motif

    Domaini

    In contrast to classical MAPKs, the TXY motif within the activation loop is replaced by the SEG motif, whose phosphorylation activates the MAP kinases.By similarity

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074298.
    HOGENOMiHOG000233020.
    HOVERGENiHBG104376.
    InParanoidiP27704.
    KOiK06855.
    OMAiEHDWPIH.
    OrthoDBiEOG780RKS.
    PhylomeDBiP27704.
    TreeFamiTF105098.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR008350. MAPK_ERK3/4.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01771. ERK3ERK4MAPK.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27704-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEKFESLMN IHGFDLGSRY MDLKPLGCGG NGLVFSAVDN DCDKRVAIKK    50
    IVLTDPQSVK HALREIKIIR RLDHDNIVKV FEILGPSGSQ LTDDVGSLTE 100
    LNSVYIVQEY METDLANVLE QGPLLEEHAR LFMYQLLRGL KYIHSANVLH 150
    RDLKPANLFI NTEDLVLKIG DFGLARIMDP HYSHKGHLSE GLVTKWYRSP 200
    RLLLSPNNYT KAIDMWAAGC IFAEMLTGKT LFAGAHELEQ MQLILESIPV 250
    VHEEDRQELL SVIPVYIRND MTEPHKPLTQ LLPGISREAL DFLEQILTFS 300
    PMDRLTAEEA LSHPYMSIYS FPTDEPISSH PFHIEDEVDD ILLMDETHSH 350
    IYNWERYHDC QFSEHDWPIH NNFDIDEVQL DPRALSDVTD EEEVQVDPRK 400
    YLDGDREKYL EDPAFDTSYS AEPCWQYPDH HENKYCDLEC SHTCNYKTRS 450
    PSYLDNLVWR ESEVNHYYEP KLIIDLSNWK EQSKDKSDKR GKSKCERNGL 500
    VKAQIALEEA SQQLAERERG QGFDFDAFIA GTVQLSAQRE SADVVDKLND 550
    LNSSVSQLEM KSLISKSVSR EKQEKGRANL AQLGALYQPS WESQFVSGGE 600
    ECFLISQFCC EVRKDEHVEK ENTYTSYLDK FFSRKEDSEM LETEPVEEGK 650
    RGERGREAGL LSSGGEFLLS RQLESIGTPQ FHSPGGSPLK SIQATLTPSA 700
    MKSSPQIPHK TYSNILKHLN 720
    Length:720
    Mass (Da):82,275
    Last modified:November 1, 1997 - v2
    Checksum:iB200B215574AB84D
    GO

    Sequence cautioni

    The sequence AAA41125.1 differs from that shown. Reason: Frameshift at position 503.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64301 mRNA. Translation: AAA41125.1. Frameshift.
    BC109380 mRNA. Translation: AAI09381.1.
    PIRiB40033.
    RefSeqiNP_113810.2. NM_031622.2.
    XP_006243471.1. XM_006243409.1.
    UniGeneiRn.88457.

    Genome annotation databases

    EnsembliENSRNOT00000013053; ENSRNOP00000013053; ENSRNOG00000009381.
    GeneIDi58840.
    KEGGirno:58840.
    UCSCiRGD:62087. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M64301 mRNA. Translation: AAA41125.1 . Frameshift.
    BC109380 mRNA. Translation: AAI09381.1 .
    PIRi B40033.
    RefSeqi NP_113810.2. NM_031622.2.
    XP_006243471.1. XM_006243409.1.
    UniGenei Rn.88457.

    3D structure databases

    ProteinModelPortali P27704.
    SMRi P27704. Positions 13-321.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000013053.

    PTM databases

    PhosphoSitei P27704.

    Proteomic databases

    PaxDbi P27704.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000013053 ; ENSRNOP00000013053 ; ENSRNOG00000009381 .
    GeneIDi 58840.
    KEGGi rno:58840.
    UCSCi RGD:62087. rat.

    Organism-specific databases

    CTDi 5597.
    RGDi 62087. Mapk6.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074298.
    HOGENOMi HOG000233020.
    HOVERGENi HBG104376.
    InParanoidi P27704.
    KOi K06855.
    OMAi EHDWPIH.
    OrthoDBi EOG780RKS.
    PhylomeDBi P27704.
    TreeFami TF105098.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 5301.

    Miscellaneous databases

    NextBioi 611410.
    PROi P27704.

    Gene expression databases

    Genevestigatori P27704.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR008350. MAPK_ERK3/4.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01771. ERK3ERK4MAPK.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "ERKs: a family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF."
      Boulton T.G., Nye S.H., Robbins D.J., Ip N.Y., Radziejewska E., Morgenbesser S.D., DePinho R.A., Panayotatos N., Cobb M.H., Yancopoulos G.D.
      Cell 65:663-675(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Brain and Liver.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Placenta.

    Entry informationi

    Entry nameiMK06_RAT
    AccessioniPrimary (citable) accession number: P27704
    Secondary accession number(s): Q32LY4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 118 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3