ID   CD82_HUMAN              Reviewed;         267 AA.
AC   P27701; D3DQN6; Q7Z2D4;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1992, sequence version 1.
DT   25-JAN-2012, entry version 99.
DE   RecName: Full=CD82 antigen;
DE   AltName: Full=C33 antigen;
DE   AltName: Full=IA4;
DE   AltName: Full=Inducible membrane protein R2;
DE   AltName: Full=Metastasis suppressor Kangai-1;
DE   AltName: Full=Suppressor of tumorigenicity 6 protein;
DE   AltName: Full=Tetraspanin-27;
DE            Short=Tspan-27;
DE   AltName: CD_antigen=CD82;
GN   Name=CD82; Synonyms=KAI1, SAR2, ST6, TSPAN27;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Peripheral blood lymphocyte;
RX   MEDLINE=91153380; PubMed=1842498; DOI=10.1002/eji.1830210219;
RA   Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.;
RT   "A new superfamily of lymphoid and melanoma cell proteins with
RT   extensive homology to Schistosoma mansoni antigen Sm23.";
RL   Eur. J. Immunol. 21:377-383(1991).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=93017900; PubMed=1401919;
RA   Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N.,
RA   Nishimura M., Hinuma Y., Yoshie O.;
RT   "C33 antigen recognized by monoclonal antibodies inhibitory to human T
RT   cell leukemia virus type 1-induced syncytium formation is a member of
RT   a new family of transmembrane proteins including CD9, CD37, CD53, and
RT   CD63.";
RL   J. Immunol. 149:2879-2886(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=95273964; PubMed=7754374; DOI=10.1126/science.7754374;
RA   Dong J.T., Barrett J.C.;
RT   "KAI1, a metastasis suppressor gene for prostate cancer on human
RT   chromosome 11p11.2.";
RL   Science 268:884-886(1995).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=97271552; PubMed=9126478; DOI=10.1006/geno.1997.4618;
RA   Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.;
RT   "Genomic organization of the human KAI1 metastasis-suppressor gene.";
RL   Genomics 41:25-32(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S.,
RA   Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W.,
RA   Korn B., Zuo D., Hu Y., LaBaer J.;
RT   "Cloning of human full open reading frames in Gateway(TM) system entry
RT   vector (pDONR201).";
RL   Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN   [6]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT VAL-241.
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN   [7]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-241.
RC   TISSUE=Colon;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [8]
RP   INTERACTION WITH IGSF8.
RX   PubMed=12750295;
RA   Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.;
RT   "EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and
RT   inhibits the migration of prostate cancer cells.";
RL   Cancer Res. 63:2665-2674(2003).
RN   [9]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Liver;
RX   PubMed=19159218; DOI=10.1021/pr8008012;
RA   Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT   "Glycoproteomics analysis of human liver tissue by combination of
RT   multiple enzyme digestion and hydrazide chemistry.";
RL   J. Proteome Res. 8:651-661(2009).
RN   [10]
RP   GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198, AND MASS
RP   SPECTROMETRY.
RC   TISSUE=Leukemic T-cell;
RX   PubMed=19349973; DOI=10.1038/nbt.1532;
RA   Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA   Schiess R., Aebersold R., Watts J.D.;
RT   "Mass-spectrometric identification and relative quantification of N-
RT   linked cell surface glycoproteins.";
RL   Nat. Biotechnol. 27:378-386(2009).
CC   -!- FUNCTION: Associates with CD4 or CD8 and delivers costimulatory
CC       signals for the TCR/CD3 pathway.
CC   -!- SUBUNIT: Interacts directly with IGSF8.
CC   -!- INTERACTION:
CC       Q8TAA9:VANGL1; NbExp=6; IntAct=EBI-682379, EBI-682393;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC   -!- TISSUE SPECIFICITY: Lymphoid specific.
CC   -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family.
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DR   EMBL; X53795; CAA37804.1; -; mRNA.
DR   EMBL; S48196; AAB23825.1; -; mRNA.
DR   EMBL; U20770; AAC50133.1; -; mRNA.
DR   EMBL; U67274; AAC51205.1; -; Genomic_DNA.
DR   EMBL; U67268; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; U67269; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; U67270; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; U67271; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; U67272; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; U67273; AAC51205.1; JOINED; Genomic_DNA.
DR   EMBL; CR542255; CAG47051.1; -; mRNA.
DR   EMBL; CH471064; EAW68063.1; -; Genomic_DNA.
DR   EMBL; CH471064; EAW68064.1; -; Genomic_DNA.
DR   EMBL; BC000726; AAH00726.1; -; mRNA.
DR   EMBL; BC001821; AAH01821.1; -; mRNA.
DR   IPI; IPI00020446; -.
DR   PIR; I38942; A46493.
DR   RefSeq; NP_001020015.1; NM_001024844.1.
DR   RefSeq; NP_002222.1; NM_002231.3.
DR   UniGene; Hs.527778; -.
DR   ProteinModelPortal; P27701; -.
DR   IntAct; P27701; 1.
DR   STRING; P27701; -.
DR   PhosphoSite; P27701; -.
DR   DMDM; 131775; -.
DR   PRIDE; P27701; -.
DR   Ensembl; ENST00000227155; ENSP00000227155; ENSG00000085117.
DR   Ensembl; ENST00000441195; ENSP00000388758; ENSG00000085117.
DR   GeneID; 3732; -.
DR   KEGG; hsa:3732; -.
DR   UCSC; uc001myc.1; human.
DR   CTD; 3732; -.
DR   GeneCards; GC11P044544; -.
DR   H-InvDB; HIX0017389; -.
DR   HGNC; HGNC:6210; CD82.
DR   HPA; CAB002508; -.
DR   HPA; HPA028900; -.
DR   MIM; 600623; gene.
DR   neXtProt; NX_P27701; -.
DR   PharmGKB; PA142672155; -.
DR   eggNOG; prNOG18880; -.
DR   HOGENOM; HBG716506; -.
DR   HOVERGEN; HBG002324; -.
DR   InParanoid; P27701; -.
DR   OMA; KCCGWVS; -.
DR   PhylomeDB; P27701; -.
DR   NextBio; 14607; -.
DR   ArrayExpress; P27701; -.
DR   Bgee; P27701; -.
DR   CleanEx; HS_CD82; -.
DR   Genevestigator; P27701; -.
DR   GermOnline; ENSG00000085117; Homo sapiens.
DR   GO; GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR018499; Tetraspanin.
DR   InterPro; IPR018503; Tetraspanin_CS.
DR   InterPro; IPR008952; Tetraspanin_EC2.
DR   InterPro; IPR000301; Tetraspanin_subgr.
DR   KO; K06509; -.
DR   Pfam; PF00335; Tetraspannin; 1.
DR   PRINTS; PR00259; TMFOUR.
DR   SUPFAM; SSF48652; Tetraspanin; 1.
DR   PROSITE; PS00421; TM4_1; 1.
PE   1: Evidence at protein level;
KW   Complete proteome; Glycoprotein; Membrane; Polymorphism;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN         1    267       CD82 antigen.
FT                                /FTId=PRO_0000219226.
FT   TOPO_DOM      1     11       Cytoplasmic (Potential).
FT   TRANSMEM     12     32       Helical; (Potential).
FT   TOPO_DOM     33     53       Extracellular (Potential).
FT   TRANSMEM     54     72       Helical; (Potential).
FT   TOPO_DOM     73     83       Cytoplasmic (Potential).
FT   TRANSMEM     84    110       Helical; (Potential).
FT   TOPO_DOM    111    228       Extracellular (Potential).
FT   TRANSMEM    229    250       Helical; (Potential).
FT   TOPO_DOM    251    267       Cytoplasmic (Potential).
FT   CARBOHYD    129    129       N-linked (GlcNAc...).
FT   CARBOHYD    157    157       N-linked (GlcNAc...) (Potential).
FT   CARBOHYD    198    198       N-linked (GlcNAc...).
FT   VARIANT     241    241       I -> V (in dbSNP:rs1139971).
FT                                /FTId=VAR_052326.
FT   CONFLICT    240    241       II -> MV (in Ref. 2; AAB23825).
SQ   SEQUENCE   267 AA;  29626 MW;  FC379855BD1CABDE CRC64;
     MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI
     GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV TAGALFYFNM GKLKQEMGGI
     VTELIRDYNS SREDSLQDAW DYVQAQVKCC GWVSFYNWTD NAELMNRPEV TYPCSCEVKG
     EEDNSLSVRK GFCEAPGNRT QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI
     IELLGMVLSI CLCRHVHSED YSKVPKY
//