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P27701 (CD82_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 118. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
CD82 antigen
Alternative name(s):
C33 antigen
IA4
Inducible membrane protein R2
Metastasis suppressor Kangai-1
Suppressor of tumorigenicity 6 protein
Tetraspanin-27
Short name=Tspan-27
CD_antigen=CD82
Gene names
Name:CD82
Synonyms:KAI1, SAR2, ST6, TSPAN27
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

Subunit structure

Interacts directly with IGSF8. Ref.10

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Lymphoid specific.

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VANGL1Q8TAA96EBI-682379,EBI-682393

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: P27701-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: P27701-2)

The sequence of this isoform differs from the canonical sequence as follows:
     88-112: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267CD82 antigen
PRO_0000219226

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221Helical; Potential
Topological domain33 – 5321Extracellular Potential
Transmembrane54 – 7219Helical; Potential
Topological domain73 – 8311Cytoplasmic Potential
Transmembrane84 – 11027Helical; Potential
Topological domain111 – 228118Extracellular Potential
Transmembrane229 – 25022Helical; Potential
Topological domain251 – 26717Cytoplasmic Potential

Amino acid modifications

Glycosylation1291N-linked (GlcNAc...) Ref.11 Ref.12
Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Ref.12

Natural variations

Alternative sequence88 – 11225Missing in isoform 2.
VSP_045656
Natural variant2411I → V. Ref.4 Ref.8 Ref.9
Corresponds to variant rs1139971 [ dbSNP | Ensembl ].
VAR_052326

Experimental info

Sequence conflict1241L → P in AAP76181. Ref.4
Sequence conflict240 – 2412II → MV in AAB23825. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: FC379855BD1CABDE

FASTA26729,626
        10         20         30         40         50         60 
MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI 

        70         80         90        100        110        120 
GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV TAGALFYFNM GKLKQEMGGI 

       130        140        150        160        170        180 
VTELIRDYNS SREDSLQDAW DYVQAQVKCC GWVSFYNWTD NAELMNRPEV TYPCSCEVKG 

       190        200        210        220        230        240 
EEDNSLSVRK GFCEAPGNRT QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI 

       250        260 
IELLGMVLSI CLCRHVHSED YSKVPKY 

« Hide

Isoform 2 [UniParc].

Checksum: E80ECE34CFFF383D
Show »

FASTA24226,818

References

« Hide 'large scale' references
[1]"A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23."
Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.
Eur. J. Immunol. 21:377-383(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
Tissue: Peripheral blood lymphocyte.
[2]"C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63."
Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N., Nishimura M., Hinuma Y., Yoshie O.
J. Immunol. 149:2879-2886(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2."
Dong J.T., Barrett J.C.
Science 268:884-886(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]Zhou G., Li S., Li H., Shen C., Li M., Xiao W., Lin L., Yang S.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT VAL-241.
[5]"Genomic organization of the human KAI1 metastasis-suppressor gene."
Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.
Genomics 41:25-32(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]"Human chromosome 11 DNA sequence and analysis including novel gene identification."
Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. expand/collapse author list , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y.
Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-241.
[9]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241.
Tissue: Colon.
[10]"EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH IGSF8.
[11]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129.
Tissue: Liver.
[12]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198.
Tissue: Leukemic T-cell.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53795 mRNA. Translation: CAA37804.1.
S48196 mRNA. Translation: AAB23825.1.
U20770 mRNA. Translation: AAC50133.1.
AY303776 mRNA. Translation: AAP76181.1.
U67274 expand/collapse EMBL AC list , U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1.
CR542255 mRNA. Translation: CAG47051.1.
AC010768 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68063.1.
CH471064 Genomic DNA. Translation: EAW68064.1.
BC000726 mRNA. Translation: AAH00726.1.
BC001821 mRNA. Translation: AAH01821.1.
PIRA46493. I38942.
RefSeqNP_001020015.1. NM_001024844.1.
NP_002222.1. NM_002231.3.
UniGeneHs.527778.

3D structure databases

ProteinModelPortalP27701.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid109935. 22 interactions.
IntActP27701. 3 interactions.
MINTMINT-1432537.
STRING9606.ENSP00000227155.

PTM databases

PhosphoSiteP27701.

Polymorphism databases

DMDM131775.

Proteomic databases

PaxDbP27701.
PRIDEP27701.

Protocols and materials databases

DNASU3732.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000227155; ENSP00000227155; ENSG00000085117. [P27701-1]
ENST00000342935; ENSP00000339686; ENSG00000085117. [P27701-2]
GeneID3732.
KEGGhsa:3732.
UCSCuc001myc.3. human. [P27701-1]

Organism-specific databases

CTD3732.
GeneCardsGC11P044586.
HGNCHGNC:6210. CD82.
HPACAB002508.
HPA028900.
MIM600623. gene.
neXtProtNX_P27701.
PharmGKBPA142672155.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG259116.
HOGENOMHOG000230651.
HOVERGENHBG002324.
InParanoidP27701.
KOK06509.
OMAVTYPCSC.
PhylomeDBP27701.
TreeFamTF352892.

Gene expression databases

ArrayExpressP27701.
BgeeP27701.
CleanExHS_CD82.
GenevestigatorP27701.

Family and domain databases

InterProIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFPIRSF002419. Tetraspanin. 1 hit.
PRINTSPR00259. TMFOUR.
SUPFAMSSF48652. SSF48652. 1 hit.
PROSITEPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD82. human.
GeneWikiCD82_(gene).
GenomeRNAi3732.
NextBio14607.
PROP27701.
SOURCESearch...

Entry information

Entry nameCD82_HUMAN
AccessionPrimary (citable) accession number: P27701
Secondary accession number(s): D3DQN6 expand/collapse secondary AC list , E9PC70, Q7Z2D4, Q7Z5N2
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries