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P27701

- CD82_HUMAN

UniProt

P27701 - CD82_HUMAN

Protein

CD82 antigen

Gene

CD82

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

    GO - Molecular functioni

    1. protein binding Source: IntAct

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD82 antigen
    Alternative name(s):
    C33 antigen
    IA4
    Inducible membrane protein R2
    Metastasis suppressor Kangai-1
    Suppressor of tumorigenicity 6 protein
    Tetraspanin-27
    Short name:
    Tspan-27
    CD_antigen: CD82
    Gene namesi
    Name:CD82
    Synonyms:KAI1, SAR2, ST6, TSPAN27
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:6210. CD82.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular vesicular exosome Source: UniProt
    2. integral component of plasma membrane Source: ProtInc
    3. plasma membrane Source: ProtInc

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA142672155.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 267267CD82 antigenPRO_0000219226Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi129 – 1291N-linked (GlcNAc...)2 Publications
    Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi198 – 1981N-linked (GlcNAc...)1 Publication

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiP27701.
    PaxDbiP27701.
    PRIDEiP27701.

    PTM databases

    PhosphoSiteiP27701.

    Expressioni

    Tissue specificityi

    Lymphoid specific.

    Gene expression databases

    ArrayExpressiP27701.
    BgeeiP27701.
    CleanExiHS_CD82.
    GenevestigatoriP27701.

    Organism-specific databases

    HPAiCAB002508.
    HPA028900.

    Interactioni

    Subunit structurei

    Interacts directly with IGSF8.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VANGL1Q8TAA96EBI-682379,EBI-682393

    Protein-protein interaction databases

    BioGridi109935. 22 interactions.
    IntActiP27701. 5 interactions.
    MINTiMINT-1432537.
    STRINGi9606.ENSP00000227155.

    Structurei

    3D structure databases

    ProteinModelPortaliP27701.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini33 – 5321ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini73 – 8311CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini111 – 228118ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini251 – 26717CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei12 – 3221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei54 – 7219HelicalSequence AnalysisAdd
    BLAST
    Transmembranei84 – 11027HelicalSequence AnalysisAdd
    BLAST
    Transmembranei229 – 25022HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tetraspanin (TM4SF) family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG259116.
    HOGENOMiHOG000230651.
    HOVERGENiHBG002324.
    InParanoidiP27701.
    KOiK06509.
    OMAiVTYPCSC.
    PhylomeDBiP27701.
    TreeFamiTF352892.

    Family and domain databases

    InterProiIPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view]
    PfamiPF00335. Tetraspannin. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002419. Tetraspanin. 1 hit.
    PRINTSiPR00259. TMFOUR.
    SUPFAMiSSF48652. SSF48652. 1 hit.
    PROSITEiPS00421. TM4_1. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P27701-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS    50
    SLRMGAYVFI GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV 100
    TAGALFYFNM GKLKQEMGGI VTELIRDYNS SREDSLQDAW DYVQAQVKCC 150
    GWVSFYNWTD NAELMNRPEV TYPCSCEVKG EEDNSLSVRK GFCEAPGNRT 200
    QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI IELLGMVLSI 250
    CLCRHVHSED YSKVPKY 267
    Length:267
    Mass (Da):29,626
    Last modified:August 1, 1992 - v1
    Checksum:iFC379855BD1CABDE
    GO
    Isoform 2 (identifier: P27701-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         88-112: Missing.

    Show »
    Length:242
    Mass (Da):26,818
    Checksum:iE80ECE34CFFF383D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti124 – 1241L → P in AAP76181. 1 PublicationCurated
    Sequence conflicti240 – 2412II → MV in AAB23825. (PubMed:1401919)Curated

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti241 – 2411I → V.3 Publications
    Corresponds to variant rs1139971 [ dbSNP | Ensembl ].
    VAR_052326

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei88 – 11225Missing in isoform 2. 1 PublicationVSP_045656Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53795 mRNA. Translation: CAA37804.1.
    S48196 mRNA. Translation: AAB23825.1.
    U20770 mRNA. Translation: AAC50133.1.
    AY303776 mRNA. Translation: AAP76181.1.
    U67274
    , U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1.
    CR542255 mRNA. Translation: CAG47051.1.
    AC010768 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68063.1.
    CH471064 Genomic DNA. Translation: EAW68064.1.
    BC000726 mRNA. Translation: AAH00726.1.
    BC001821 mRNA. Translation: AAH01821.1.
    CCDSiCCDS31469.1. [P27701-2]
    CCDS7909.1. [P27701-1]
    PIRiI38942. A46493.
    RefSeqiNP_001020015.1. NM_001024844.1. [P27701-2]
    NP_002222.1. NM_002231.3. [P27701-1]
    XP_006718286.1. XM_006718223.1. [P27701-1]
    UniGeneiHs.527778.

    Genome annotation databases

    EnsembliENST00000227155; ENSP00000227155; ENSG00000085117. [P27701-1]
    ENST00000342935; ENSP00000339686; ENSG00000085117. [P27701-2]
    GeneIDi3732.
    KEGGihsa:3732.
    UCSCiuc001myc.3. human. [P27701-1]

    Polymorphism databases

    DMDMi131775.

    Keywords - Coding sequence diversityi

    Alternative splicing, Polymorphism

    Cross-referencesi

    Web resourcesi

    Atlas of Genetics and Cytogenetics in Oncology and Haematology

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53795 mRNA. Translation: CAA37804.1 .
    S48196 mRNA. Translation: AAB23825.1 .
    U20770 mRNA. Translation: AAC50133.1 .
    AY303776 mRNA. Translation: AAP76181.1 .
    U67274
    , U67268 , U67269 , U67270 , U67271 , U67272 , U67273 Genomic DNA. Translation: AAC51205.1 .
    CR542255 mRNA. Translation: CAG47051.1 .
    AC010768 Genomic DNA. No translation available.
    CH471064 Genomic DNA. Translation: EAW68063.1 .
    CH471064 Genomic DNA. Translation: EAW68064.1 .
    BC000726 mRNA. Translation: AAH00726.1 .
    BC001821 mRNA. Translation: AAH01821.1 .
    CCDSi CCDS31469.1. [P27701-2 ]
    CCDS7909.1. [P27701-1 ]
    PIRi I38942. A46493.
    RefSeqi NP_001020015.1. NM_001024844.1. [P27701-2 ]
    NP_002222.1. NM_002231.3. [P27701-1 ]
    XP_006718286.1. XM_006718223.1. [P27701-1 ]
    UniGenei Hs.527778.

    3D structure databases

    ProteinModelPortali P27701.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 109935. 22 interactions.
    IntActi P27701. 5 interactions.
    MINTi MINT-1432537.
    STRINGi 9606.ENSP00000227155.

    PTM databases

    PhosphoSitei P27701.

    Polymorphism databases

    DMDMi 131775.

    Proteomic databases

    MaxQBi P27701.
    PaxDbi P27701.
    PRIDEi P27701.

    Protocols and materials databases

    DNASUi 3732.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000227155 ; ENSP00000227155 ; ENSG00000085117 . [P27701-1 ]
    ENST00000342935 ; ENSP00000339686 ; ENSG00000085117 . [P27701-2 ]
    GeneIDi 3732.
    KEGGi hsa:3732.
    UCSCi uc001myc.3. human. [P27701-1 ]

    Organism-specific databases

    CTDi 3732.
    GeneCardsi GC11P044586.
    HGNCi HGNC:6210. CD82.
    HPAi CAB002508.
    HPA028900.
    MIMi 600623. gene.
    neXtProti NX_P27701.
    PharmGKBi PA142672155.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG259116.
    HOGENOMi HOG000230651.
    HOVERGENi HBG002324.
    InParanoidi P27701.
    KOi K06509.
    OMAi VTYPCSC.
    PhylomeDBi P27701.
    TreeFami TF352892.

    Miscellaneous databases

    ChiTaRSi CD82. human.
    GeneWikii CD82_(gene).
    GenomeRNAii 3732.
    NextBioi 14607.
    PROi P27701.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27701.
    Bgeei P27701.
    CleanExi HS_CD82.
    Genevestigatori P27701.

    Family and domain databases

    InterProi IPR000301. Tetraspanin.
    IPR018499. Tetraspanin/Peripherin.
    IPR018503. Tetraspanin_CS.
    IPR008952. Tetraspanin_EC2.
    [Graphical view ]
    Pfami PF00335. Tetraspannin. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002419. Tetraspanin. 1 hit.
    PRINTSi PR00259. TMFOUR.
    SUPFAMi SSF48652. SSF48652. 1 hit.
    PROSITEi PS00421. TM4_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23."
      Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.
      Eur. J. Immunol. 21:377-383(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Tissue: Peripheral blood lymphocyte.
    2. "C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63."
      Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N., Nishimura M., Hinuma Y., Yoshie O.
      J. Immunol. 149:2879-2886(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. "KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2."
      Dong J.T., Barrett J.C.
      Science 268:884-886(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    4. Zhou G., Li S., Li H., Shen C., Li M., Xiao W., Lin L., Yang S.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT VAL-241.
    5. "Genomic organization of the human KAI1 metastasis-suppressor gene."
      Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.
      Genomics 41:25-32(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-241.
    9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241.
      Tissue: Colon.
    10. "EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
      Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
      Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH IGSF8.
    11. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
      Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
      J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129.
      Tissue: Liver.
    12. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
      Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
      Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198.
      Tissue: Leukemic T-cell.

    Entry informationi

    Entry nameiCD82_HUMAN
    AccessioniPrimary (citable) accession number: P27701
    Secondary accession number(s): D3DQN6
    , E9PC70, Q7Z2D4, Q7Z5N2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 122 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3