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Protein

CD82 antigen

Gene

CD82

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
CD82 antigen
Alternative name(s):
C33 antigen
IA4
Inducible membrane protein R2
Metastasis suppressor Kangai-1
Suppressor of tumorigenicity 6 protein
Tetraspanin-27
Short name:
Tspan-27
CD_antigen: CD82
Gene namesi
Name:CD82
Synonyms:KAI1, SAR2, ST6, TSPAN27
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:6210. CD82.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1111CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei12 – 3221HelicalSequence AnalysisAdd
BLAST
Topological domaini33 – 5321ExtracellularSequence AnalysisAdd
BLAST
Transmembranei54 – 7219HelicalSequence AnalysisAdd
BLAST
Topological domaini73 – 8311CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei84 – 11027HelicalSequence AnalysisAdd
BLAST
Topological domaini111 – 228118ExtracellularSequence AnalysisAdd
BLAST
Transmembranei229 – 25022HelicalSequence AnalysisAdd
BLAST
Topological domaini251 – 26717CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • extracellular exosome Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • plasma membrane Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA142672155.

Polymorphism and mutation databases

BioMutaiCD82.
DMDMi131775.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 267267CD82 antigenPRO_0000219226Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi129 – 1291N-linked (GlcNAc...)2 Publications
Glycosylationi157 – 1571N-linked (GlcNAc...)Sequence Analysis
Glycosylationi198 – 1981N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP27701.
PaxDbiP27701.
PRIDEiP27701.

PTM databases

PhosphoSiteiP27701.

Expressioni

Tissue specificityi

Lymphoid specific.

Gene expression databases

BgeeiP27701.
CleanExiHS_CD82.
ExpressionAtlasiP27701. baseline and differential.
GenevisibleiP27701. HS.

Organism-specific databases

HPAiCAB002508.
HPA028900.
HPA061418.

Interactioni

Subunit structurei

Interacts directly with IGSF8.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VANGL1Q8TAA96EBI-682379,EBI-682393

Protein-protein interaction databases

BioGridi109935. 22 interactions.
IntActiP27701. 5 interactions.
MINTiMINT-1432537.
STRINGi9606.ENSP00000227155.

Structurei

3D structure databases

ProteinModelPortaliP27701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tetraspanin (TM4SF) family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG259116.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP27701.
KOiK06509.
OMAiVTYPCSC.
PhylomeDBiP27701.
TreeFamiTF352892.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P27701-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS
60 70 80 90 100
SLRMGAYVFI GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV
110 120 130 140 150
TAGALFYFNM GKLKQEMGGI VTELIRDYNS SREDSLQDAW DYVQAQVKCC
160 170 180 190 200
GWVSFYNWTD NAELMNRPEV TYPCSCEVKG EEDNSLSVRK GFCEAPGNRT
210 220 230 240 250
QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI IELLGMVLSI
260
CLCRHVHSED YSKVPKY
Length:267
Mass (Da):29,626
Last modified:August 1, 1992 - v1
Checksum:iFC379855BD1CABDE
GO
Isoform 2 (identifier: P27701-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     88-112: Missing.

Show »
Length:242
Mass (Da):26,818
Checksum:iE80ECE34CFFF383D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti124 – 1241L → P in AAP76181 (Ref. 4) Curated
Sequence conflicti240 – 2412II → MV in AAB23825 (PubMed:1401919).Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti241 – 2411I → V.3 Publications
Corresponds to variant rs1139971 [ dbSNP | Ensembl ].
VAR_052326

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei88 – 11225Missing in isoform 2. 1 PublicationVSP_045656Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53795 mRNA. Translation: CAA37804.1.
S48196 mRNA. Translation: AAB23825.1.
U20770 mRNA. Translation: AAC50133.1.
AY303776 mRNA. Translation: AAP76181.1.
U67274
, U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1.
CR542255 mRNA. Translation: CAG47051.1.
AC010768 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68063.1.
CH471064 Genomic DNA. Translation: EAW68064.1.
BC000726 mRNA. Translation: AAH00726.1.
BC001821 mRNA. Translation: AAH01821.1.
CCDSiCCDS31469.1. [P27701-2]
CCDS7909.1. [P27701-1]
PIRiI38942. A46493.
RefSeqiNP_001020015.1. NM_001024844.1. [P27701-2]
NP_002222.1. NM_002231.3. [P27701-1]
XP_006718286.1. XM_006718223.1. [P27701-1]
UniGeneiHs.527778.

Genome annotation databases

EnsembliENST00000227155; ENSP00000227155; ENSG00000085117. [P27701-1]
ENST00000342935; ENSP00000339686; ENSG00000085117. [P27701-2]
GeneIDi3732.
KEGGihsa:3732.
UCSCiuc001myc.3. human. [P27701-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53795 mRNA. Translation: CAA37804.1.
S48196 mRNA. Translation: AAB23825.1.
U20770 mRNA. Translation: AAC50133.1.
AY303776 mRNA. Translation: AAP76181.1.
U67274
, U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1.
CR542255 mRNA. Translation: CAG47051.1.
AC010768 Genomic DNA. No translation available.
CH471064 Genomic DNA. Translation: EAW68063.1.
CH471064 Genomic DNA. Translation: EAW68064.1.
BC000726 mRNA. Translation: AAH00726.1.
BC001821 mRNA. Translation: AAH01821.1.
CCDSiCCDS31469.1. [P27701-2]
CCDS7909.1. [P27701-1]
PIRiI38942. A46493.
RefSeqiNP_001020015.1. NM_001024844.1. [P27701-2]
NP_002222.1. NM_002231.3. [P27701-1]
XP_006718286.1. XM_006718223.1. [P27701-1]
UniGeneiHs.527778.

3D structure databases

ProteinModelPortaliP27701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi109935. 22 interactions.
IntActiP27701. 5 interactions.
MINTiMINT-1432537.
STRINGi9606.ENSP00000227155.

PTM databases

PhosphoSiteiP27701.

Polymorphism and mutation databases

BioMutaiCD82.
DMDMi131775.

Proteomic databases

MaxQBiP27701.
PaxDbiP27701.
PRIDEiP27701.

Protocols and materials databases

DNASUi3732.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000227155; ENSP00000227155; ENSG00000085117. [P27701-1]
ENST00000342935; ENSP00000339686; ENSG00000085117. [P27701-2]
GeneIDi3732.
KEGGihsa:3732.
UCSCiuc001myc.3. human. [P27701-1]

Organism-specific databases

CTDi3732.
GeneCardsiGC11P044586.
HGNCiHGNC:6210. CD82.
HPAiCAB002508.
HPA028900.
HPA061418.
MIMi600623. gene.
neXtProtiNX_P27701.
PharmGKBiPA142672155.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG259116.
GeneTreeiENSGT00760000118983.
HOGENOMiHOG000230651.
HOVERGENiHBG002324.
InParanoidiP27701.
KOiK06509.
OMAiVTYPCSC.
PhylomeDBiP27701.
TreeFamiTF352892.

Miscellaneous databases

ChiTaRSiCD82. human.
GeneWikiiCD82_(gene).
GenomeRNAii3732.
NextBioi14607.
PROiP27701.
SOURCEiSearch...

Gene expression databases

BgeeiP27701.
CleanExiHS_CD82.
ExpressionAtlasiP27701. baseline and differential.
GenevisibleiP27701. HS.

Family and domain databases

InterProiIPR000301. Tetraspanin.
IPR018499. Tetraspanin/Peripherin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
[Graphical view]
PfamiPF00335. Tetraspannin. 1 hit.
[Graphical view]
PIRSFiPIRSF002419. Tetraspanin. 1 hit.
PRINTSiPR00259. TMFOUR.
SUPFAMiSSF48652. SSF48652. 1 hit.
PROSITEiPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23."
    Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.
    Eur. J. Immunol. 21:377-383(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Tissue: Peripheral blood lymphocyte.
  2. "C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63."
    Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N., Nishimura M., Hinuma Y., Yoshie O.
    J. Immunol. 149:2879-2886(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2."
    Dong J.T., Barrett J.C.
    Science 268:884-886(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. Zhou G., Li S., Li H., Shen C., Li M., Xiao W., Lin L., Yang S.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT VAL-241.
  5. "Genomic organization of the human KAI1 metastasis-suppressor gene."
    Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.
    Genomics 41:25-32(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  6. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-241.
  9. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241.
    Tissue: Colon.
  10. "EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
    Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
    Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH IGSF8.
  11. "Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
    Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
    J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129.
    Tissue: Liver.
  12. "Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
    Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
    Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198.
    Tissue: Leukemic T-cell.

Entry informationi

Entry nameiCD82_HUMAN
AccessioniPrimary (citable) accession number: P27701
Secondary accession number(s): D3DQN6
, E9PC70, Q7Z2D4, Q7Z5N2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 24, 2015
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human cell differentiation molecules
    CD nomenclature of surface proteins of human leucocytes and list of entries
  2. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  3. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  4. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  5. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.