P27701 (CD82_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD82 antigen

Alternative name(s):
C33 antigen
IA4
Inducible membrane protein R2
Metastasis suppressor Kangai-1
Suppressor of tumorigenicity 6 protein
Tetraspanin-27
Short name=Tspan-27
CD_antigen=CD82

Gene names

Name:	CD82
Synonyms:	KAI1, SAR2, ST6, TSPAN27

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	267 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.
Subunit structure	Interacts directly with IGSF8. Ref.10
Subcellular location	Membrane; Multi-pass membrane protein.
Tissue specificity	Lymphoid specific.
Sequence similarities	Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Transmembrane Transmembrane helix
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to plasma membrane Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VANGL1	Q8TAA9	6	EBI-682379,EBI-682393

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P27701-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P27701-2)

The sequence of this isoform differs from the canonical sequence as follows:
     88-112: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 267	267	CD82 antigen		PRO_0000219226
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 53	21	Extracellular Potential
	Transmembrane	54 – 72	19	Helical; Potential
	Topological domain	73 – 83	11	Cytoplasmic Potential
	Transmembrane	84 – 110	27	Helical; Potential
	Topological domain	111 – 228	118	Extracellular Potential
	Transmembrane	229 – 250	22	Helical; Potential
	Topological domain	251 – 267	17	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	129	1	N-linked (GlcNAc...) Ref.11 Ref.12
	Glycosylation	157	1	N-linked (GlcNAc...) Potential
	Glycosylation	198	1	N-linked (GlcNAc...) Ref.12
Natural variations
	Alternative sequence	88 – 112	25	Missing in isoform 2.		VSP_045656
	Natural variant	241	1	I → V. Ref.4 Ref.8 Ref.9 Corresponds to variant rs1139971 [ dbSNP | Ensembl ].		VAR_052326
Experimental info
	Sequence conflict	124	1	L → P in AAP76181. Ref.4
	Sequence conflict	240 – 241	2	II → MV in AAB23825. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: FC379855BD1CABDE	FASTA	267	29,626
	10 20 30 40 50 60 MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI 70 80 90 100 110 120 GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV TAGALFYFNM GKLKQEMGGI 130 140 150 160 170 180 VTELIRDYNS SREDSLQDAW DYVQAQVKCC GWVSFYNWTD NAELMNRPEV TYPCSCEVKG 190 200 210 220 230 240 EEDNSLSVRK GFCEAPGNRT QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI 250 260 IELLGMVLSI CLCRHVHSED YSKVPKY
	Isoform 2 [UniParc]. Checksum: E80ECE34CFFF383D	FASTA	242	26,818
	10 20 30 40 50 60 MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI 70 80 90 100 110 120 GVGAVTMLMG FLGCIGAVNE VRCLLGLLKQ EMGGIVTELI RDYNSSREDS LQDAWDYVQA 130 140 150 160 170 180 QVKCCGWVSF YNWTDNAELM NRPEVTYPCS CEVKGEEDNS LSVRKGFCEA PGNRTQSGNH 190 200 210 220 230 240 PEDWPVYQEG CMEKVQAWLQ ENLGIILGVG VGVAIIELLG MVLSICLCRH VHSEDYSKVP KY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23." Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T. Eur. J. Immunol. 21:377-383(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Peripheral blood lymphocyte.
[2]	"C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63." Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N., Nishimura M., Hinuma Y., Yoshie O. J. Immunol. 149:2879-2886(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2." Dong J.T., Barrett J.C. Science 268:884-886(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[4]	Zhou G., Li S., Li H., Shen C., Li M., Xiao W., Lin L., Yang S. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), VARIANT VAL-241.
[5]	"Genomic organization of the human KAI1 metastasis-suppressor gene." Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T. Genomics 41:25-32(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[6]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
[7]	"Human chromosome 11 DNA sequence and analysis including novel gene identification." Taylor T.D., Noguchi H., Totoki Y., Toyoda A., Kuroki Y., Dewar K., Lloyd C., Itoh T., Takeda T., Kim D.-W., She X., Barlow K.F., Bloom T., Bruford E., Chang J.L., Cuomo C.A., Eichler E., FitzGerald M.G. , Jaffe D.B., LaButti K., Nicol R., Park H.-S., Seaman C., Sougnez C., Yang X., Zimmer A.R., Zody M.C., Birren B.W., Nusbaum C., Fujiyama A., Hattori M., Rogers J., Lander E.S., Sakaki Y. Nature 440:497-500(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[8]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-241.
[9]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), VARIANT VAL-241. Tissue: Colon.
[10]	"EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells." Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L. Cancer Res. 63:2665-2674(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IGSF8.
[11]	"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry." Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H. J. Proteome Res. 8:651-661(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129, MASS SPECTROMETRY. Tissue: Liver.
[12]	"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins." Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D. Nat. Biotechnol. 27:378-386(2009) [PubMed] [Europe PMC] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198, MASS SPECTROMETRY. Tissue: Leukemic T-cell.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53795 mRNA. Translation: CAA37804.1. S48196 mRNA. Translation: AAB23825.1. U20770 mRNA. Translation: AAC50133.1. AY303776 mRNA. Translation: AAP76181.1. U67274 , U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1. CR542255 mRNA. Translation: CAG47051.1. AC010768 Genomic DNA. No translation available. CH471064 Genomic DNA. Translation: EAW68063.1. CH471064 Genomic DNA. Translation: EAW68064.1. BC000726 mRNA. Translation: AAH00726.1. BC001821 mRNA. Translation: AAH01821.1.
IPI	IPI00020446. IPI00385291.
PIR	A46493. I38942.
RefSeq	NP_001020015.1. NM_001024844.1. NP_002222.1. NM_002231.3.
UniGene	Hs.527778.
3D structure databases
ProteinModelPortal	P27701.
ModBase	Search...
Protein-protein interaction databases
IntAct	P27701. 1 interaction.
STRING	9606.ENSP00000227155.
PTM databases
PhosphoSite	P27701.
Polymorphism databases
DMDM	131775.
Proteomic databases
PaxDb	P27701.
PRIDE	P27701.
Protocols and materials databases
DNASU	3732.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000227155; ENSP00000227155; ENSG00000085117. ENST00000342935; ENSP00000339686; ENSG00000085117.
GeneID	3732.
KEGG	hsa:3732.
UCSC	uc001myc.3. human.
Organism-specific databases
CTD	3732.
GeneCards	GC11P044586.
HGNC	HGNC:6210. CD82.
HPA	CAB002508. HPA028900.
MIM	600623. gene.
neXtProt	NX_P27701.
PharmGKB	PA142672155.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG259116.
HOGENOM	HOG000230651.
HOVERGEN	HBG002324.
InParanoid	P27701.
KO	K06509.
OMA	CGWVSFY.
PhylomeDB	P27701.
Gene expression databases
ArrayExpress	P27701.
Bgee	P27701.
CleanEx	HS_CD82.
Genevestigator	P27701.
GermOnline	ENSG00000085117. Homo sapiens.
Family and domain databases
InterPro	IPR000301. Tetraspanin. IPR018499. Tetraspanin/Peripherin. IPR018503. Tetraspanin_CS. IPR008952. Tetraspanin_EC2. [Graphical view]
Pfam	PF00335. Tetraspannin. 1 hit. [Graphical view]
PIRSF	PIRSF002419. Tetraspanin. 1 hit.
PRINTS	PR00259. TMFOUR.
SUPFAM	SSF48652. Tetraspanin. 1 hit.
PROSITE	PS00421. TM4_1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	CD82. human.
GenomeRNAi	3732.
NextBio	14607.
SOURCE	Search...

Entry information

Entry name

CD82_HUMAN

Accession

Primary (citable) accession number: P27701
Secondary accession number(s): D3DQN6 , E9PC70, Q7Z2D4, Q7Z5N2

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 111 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.