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Reviewed, UniProtKB/Swiss-Prot P27701 (CD82_HUMAN)

Last modified February 9, 2010. Version 85. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    CD82 antigen
Alternative name(s):
    Inducible membrane protein R2
    C33 antigen
    IA4
    Metastasis suppressor Kangai-1
    Suppressor of tumorigenicity 6 protein
    Tetraspanin-27
      Short name=Tspan-27
    CD_antigen=CD82
Gene names
Name: CD82
Synonyms: KAI1, SAR2, ST6, TSPAN27
OrganismHomo sapiens (Human) [Complete proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length267 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Associates with CD4 or CD8 and delivers costimulatory signals for the TCR/CD3 pathway.

Subunit structure

Interacts directly with IGSF8. Ref.8

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Lymphoid specific.

Sequence similarities

Belongs to the tetraspanin (TM4SF) family.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainTransmembrane
   PTMGlycoprotein
   Technical termComplete proteome
Gene Ontology (GO)
   Cellular componentintegral to plasma membrane Ref.1

Traceable author statement. Source: ProtInc

   Molecular functionprotein binding

Inferred from physical interaction. Source: IntAct

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VANGL1Q8TAA93EBI-682379,EBI-682393

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 267267CD82 antigen
PRO_0000219226

Regions

Topological domain1 – 1111Cytoplasmic Potential
Transmembrane12 – 3221 Potential
Topological domain33 – 5321Extracellular Potential
Transmembrane54 – 7219 Potential
Topological domain73 – 8311Cytoplasmic Potential
Transmembrane84 – 11027 Potential
Topological domain111 – 228118Extracellular Potential
Transmembrane229 – 25022 Potential
Topological domain251 – 26717Cytoplasmic Potential

Amino acid modifications

Glycosylation1291N-linked (GlcNAc...) Ref.9 Ref.10
Glycosylation1571N-linked (GlcNAc...) Potential
Glycosylation1981N-linked (GlcNAc...) Ref.10

Natural variations

Natural variant2411I → V: dbSNP rs1139971. Ref.7
VAR_052326

Experimental info

Sequence conflict240 – 2412II → MV in AAB23825. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P27701-1 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: FC379855BD1CABDE

FASTA26729,626
        10         20         30         40         50         60 
MGSACIKVTK YFLFLFNLIF FILGAVILGF GVWILADKSS FISVLQTSSS SLRMGAYVFI 

        70         80         90        100        110        120 
GVGAVTMLMG FLGCIGAVNE VRCLLGLYFA FLLLILIAQV TAGALFYFNM GKLKQEMGGI 

       130        140        150        160        170        180 
VTELIRDYNS SREDSLQDAW DYVQAQVKCC GWVSFYNWTD NAELMNRPEV TYPCSCEVKG 

       190        200        210        220        230        240 
EEDNSLSVRK GFCEAPGNRT QSGNHPEDWP VYQEGCMEKV QAWLQENLGI ILGVGVGVAI 

       250        260 
IELLGMVLSI CLCRHVHSED YSKVPKY 

« Hide

References

« Hide 'large scale' references
[1]"A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23."
Gaugitsch H.W., Hofer E., Huber N.E., Schnabl E., Baumruker T.
Eur. J. Immunol. 21:377-383(1991) [PubMed: 1842498] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Peripheral blood lymphocyte.
[2]"C33 antigen recognized by monoclonal antibodies inhibitory to human T cell leukemia virus type 1-induced syncytium formation is a member of a new family of transmembrane proteins including CD9, CD37, CD53, and CD63."
Imai T., Fukudome K., Takagi S., Nagira M., Furuse M., Fukuhara N., Nishimura M., Hinuma Y., Yoshie O.
J. Immunol. 149:2879-2886(1992) [PubMed: 1401919] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"KAI1, a metastasis suppressor gene for prostate cancer on human chromosome 11p11.2."
Dong J.T., Barrett J.C.
Science 268:884-886(1995) [PubMed: 7754374] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[4]"Genomic organization of the human KAI1 metastasis-suppressor gene."
Dong J.T., Isaacs W.B., Barrett J.C., Isaacs J.T.
Genomics 41:25-32(1997) [PubMed: 9126478] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], VARIANT VAL-241.
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], VARIANT VAL-241.
Tissue: Colon.
[8]"EWI2/PGRL associates with the metastasis suppressor KAI1/CD82 and inhibits the migration of prostate cancer cells."
Zhang X.A., Lane W.S., Charrin S., Rubinstein E., Liu L.
Cancer Res. 63:2665-2674(2003) [PubMed: 12750295] [Abstract]
Cited for: INTERACTION WITH IGSF8.
[9]"Glycoproteomics analysis of human liver tissue by combination of multiple enzyme digestion and hydrazide chemistry."
Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.
J. Proteome Res. 8:651-661(2009) [PubMed: 19159218] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129, MASS SPECTROMETRY.
Tissue: Liver.
[10]"Mass-spectrometric identification and relative quantification of N-linked cell surface glycoproteins."
Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M., Schiess R., Aebersold R., Watts J.D.
Nat. Biotechnol. 27:378-386(2009) [PubMed: 19349973] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-129 AND ASN-198, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53795 mRNA. Translation: CAA37804.1.
S48196 mRNA. Translation: AAB23825.1.
U20770 mRNA. Translation: AAC50133.1.
U67274 expand/collapse EMBL AC list , U67268, U67269, U67270, U67271, U67272, U67273 Genomic DNA. Translation: AAC51205.1.
CR542255 mRNA. Translation: CAG47051.1.
CH471064 Genomic DNA. Translation: EAW68063.1.
BC000726 mRNA. Translation: AAH00726.1.
BC001821 mRNA. Translation: AAH01821.1.
IPIIPI00020446.
PIRA46493. I38942.
RefSeqNP_001020015.1.
NP_002222.1.
UniGeneHs.527778

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

IntActP27701. 1 interaction.
STRINGP27701.

PTM databases

PhosphoSiteP27701.

Proteomic databases

PRIDEP27701.

Genome annotation databases

EnsemblENST00000227155; ENSP00000227155; ENSG00000085117; Homo sapiens. [Genome view]
ENST00000441195; ENSP00000388758; ENSG00000085117; Homo sapiens. [Genome view]
GeneID3732.
KEGGhsa:3732.
UCSCuc001myc.1. human.

Organism-specific databases

CTD3732.
GeneCardsGC11P044544.
H-InvDBHIX0017389.
HGNCHGNC:6210. CD82.
HPACAB002508.
MIM600623. gene.
PharmGKBPA142672155.
GenAtlasSearch...

Phylogenomic databases

eggNOGprNOG18880.
HOGENOMHBG716506.
HOVERGENP27701.
InParanoidP27701.
OMAKCCGWVS.
OrthoDBEOG9BVVDJ.
PhylomeDBP27701.

Gene expression databases

ArrayExpressP27701.
BgeeP27701.
CleanExHS_CD82.
GenevestigatorP27701.
GermOnlineENSG00000085117. Homo sapiens.

Family and domain databases

InterProIPR018499. Tetraspanin.
IPR018503. Tetraspanin_CS.
IPR008952. Tetraspanin_EC2.
IPR000301. Tetraspanin_sub.
[Graphical view]
PfamPF00335. Tetraspannin. 1 hit.
[Graphical view]
PRINTSPR00259. TMFOUR.
PROSITEPS00421. TM4_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio14607.
SOURCESearch...

Entry information

Entry nameCD82_HUMAN
AccessionPrimary (citable) accession number: P27701
Secondary accession number(s): Q7Z2D4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 9, 2010
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents