P27697 (COQ8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable serine/threonine-protein kinase COQ8, mitochondrial
EC=2.7.11.-

Alternative name(s):
Activity of bc1 complex protein 1
Coenzyme Q protein 8
Ubiquinone biosynthesis protein COQ8

Gene names

Name:	COQ8
Synonyms:	ABC1
Ordered Locus Names:	YGL119W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Putative protein kinase involved in the biosynthesis of coenzyme Q. Affects the molecular organization and function of COQ3, probably through kinase activity towards COQ3. Ref.1 Ref.4 Ref.5 Ref.7 Ref.9 Ref.11 Ref.12
Pathway	Cofactor biosynthesis; ubiquinone biosynthesis.
Subunit structure	Forms homopolymers. Predominantly associated with a complex of about 500 kDa. Ref.11
Subcellular location	Mitochondrion inner membrane; Peripheral membrane protein; Matrix side Ref.7 Ref.10 Ref.11.
Disruption phenotype	Decreases COQ3 O-methyltransferase activity. Ref.6
Miscellaneous	Present with 952 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the protein kinase superfamily. ADCK protein kinase family. Contains 1 protein kinase domain.
Caution	Was originally (Ref.1) isolated as a multicopy suppressor of a yeast strain harboring a mutation in a cytochrome b translational activator (cbs2-223). But more recently it has been shown (Ref.9) that a tRNA(Trp) gene located downstream of COQ8 mediates suppression of the cbs2-223 mutation. The inability of COQ8 to suppress the cbs2-223 allele in multicopy indicates it may not be a chaperone as previously reported.

Ontologies

Keywords
Biological process	Ubiquinone biosynthesis
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	aerobic respiration Inferred from mutant phenotype Ref.1. Source: SGD ubiquinone biosynthetic process Inferred from mutant phenotype Ref.7. Source: SGD
Cellular component	extrinsic to mitochondrial inner membrane Inferred from direct assay. Source: SGD mitochondrial matrix Inferred from direct assay Ref.11. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCT4	P39078	1	EBI-2013,EBI-19054
SSA1	P10591	1	EBI-2013,EBI-8591

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 29	29	Mitochondrion Potential
	Chain	30 – 501	472	Probable serine/threonine-protein kinase COQ8, mitochondrial		PRO_0000000260
Regions
	Domain	188 – 501	314	Protein kinase
	Nucleotide binding	194 – 202	9	ATP By similarity
Sites
	Active site	346	1	Proton acceptor By similarity
	Binding site	216	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27697 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: C91D1EEE5A7ACC9C	FASTA	501	56,742
	10 20 30 40 50 60 MVTNMVKLRN LRRLYCSSRL LRTIQNGRIS SVSSISLSKK YTTKSAKEGE ENVERKHEEE 70 80 90 100 110 120 KKDTLKSSSV PTSRISRLFH YGSLAAGVGM NAAAKGISEV AKGNSPTWKS LILSDSNIDR 130 140 150 160 170 180 ITNKFSKMRG VALKIGQLLS FQDEKVLPKE LYEILSRVQN SANHMPQRQL EKVMAKELGA 190 200 210 220 230 240 NWKTKFSKFD KIPMAAASIG QVHAAELPSG QRVVVKIQYP GVKESIDSDL NSLLMLLTAS 250 260 270 280 290 300 SLLPKGLFLD KTIANARTEL KWECDYNREA RALQKFEALL KDDPAFEVPH VFPEYTTDNI 310 320 330 340 350 360 ITMTRMEGTE IMKLPKASQE TKNFISENIM RLCLEEIATF KYMQTDPNWA NFLYNGRTKK 370 380 390 400 410 420 IELLDFGASR PFAEDFILKY RKLLTYATLR DRKGAYEMSV QLGYLTGLES QSMKDAHVDS 430 440 450 460 470 480 VLTLGEPFRG DVDKSFDFKD QTVSDRIRGN IGLMLNERLC PPPEETYSLH RKFSGIFLLC 490 500 ARMGASVHCA KLFKEIFAYK V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"ABC1, a novel yeast nuclear gene has a dual function in mitochondria: it suppresses a cytochrome b mRNA translation defect and is essential for the electron transfer in the bc 1 complex." Bousquet I., Dujardin G., Slonimski P.P. EMBO J. 10:2023-2031(1991) [PubMed: 1648478] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: S9-12/50.
[2]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"The nuclear ABC1 gene is essential for the correct conformation and functioning of the cytochrome bc1 complex and the neighbouring complexes II and IV in the mitochondrial respiratory chain." Brasseur G., Tron G., Dujardin G., Slonimski P.P., Brivet-Chevillotte P. Eur. J. Biochem. 246:103-111(1997) [PubMed: 9210471] [Abstract] Cited for: FUNCTION.
[5]	"Sensitivity to treatment with polyunsaturated fatty acids is a general characteristic of the ubiquinone-deficient yeast coq mutants." Poon W.W., Do T.Q., Marbois B.N., Clarke C.F. Mol. Aspects Med. 18:S121-S127(1997) [PubMed: 9266513] [Abstract] Cited for: FUNCTION.
[6]	"Genetic evidence for a multi-subunit complex in the O-methyltransferase steps of coenzyme Q biosynthesis." Hsu A.Y., Do T.Q., Lee P.T., Clarke C.F. Biochim. Biophys. Acta 1484:287-297(2000) [PubMed: 10760477] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"A defect in coenzyme Q biosynthesis is responsible for the respiratory deficiency in Saccharomyces cerevisiae abc1 mutants." Do T.Q., Hsu A.Y., Jonassen T., Lee P.T., Clarke C.F. J. Biol. Chem. 276:18161-18168(2001) [PubMed: 11279158] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A tRNA(TRP) gene mediates the suppression of cbs2-223 previously attributed to ABC1/COQ8." Hsieh E.J., Dinoso J.B., Clarke C.F. Biochem. Biophys. Res. Commun. 317:648-653(2004) [PubMed: 15063807] [Abstract] Cited for: FUNCTION.
[10]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed: 16823961] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[11]	"Ubiquinone biosynthesis in Saccharomyces cerevisiae: the molecular organization of O-methylase Coq3p depends on Abc1p/Coq8p." Tauche A., Krause-Buchholz U., Rodel G. FEMS Yeast Res. 8:1263-1275(2008) [PubMed: 18801050] [Abstract] Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[12]	"Hydroxylation of demethoxy-Q6 constitutes a control point in yeast coenzyme Q6 biosynthesis." Padilla S., Tran U.C., Jimenez-Hidalgo M., Lopez-Martin J.M., Martin-Montalvo A., Clarke C.F., Navas P., Santos-Ocana C. Cell. Mol. Life Sci. 66:173-186(2009) [PubMed: 19002377] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59027 Genomic DNA. Translation: CAA41759.1. Z72641 Genomic DNA. Translation: CAA96827.1. BK006941 Genomic DNA. Translation: DAA07990.1.
PIR	S16711.
RefSeq	NP_011396.1. NM_001180984.1.
3D structure databases
ProteinModelPortal	P27697.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5113N.
IntAct	P27697. 3 interactions.
MINT	MINT-575458.
STRING	P27697.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGL119W; YGL119W; YGL119W.
GeneID	852758.
KEGG	sce:YGL119W.
NMPDR	fig|4932.3.peg.2501.
Organism-specific databases
CYGD	YGL119w.
SGD	S000003087. COQ8.
Phylogenomic databases
eggNOG	fuNOG07198.
GeneTree	EFGT00050000002124.
HOGENOM	HBG381628.
OMA	FYVPEVI.
OrthoDB	EOG4VHPG1.
Gene expression databases
ArrayExpress	P27697.
Genevestigator	P27697.
GermOnline	YGL119W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004147. ABC_1. IPR011009. Kinase-like_dom. [Graphical view]
KO	K08869.
Pfam	PF03109. ABC1. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. False negative. [Graphical view]
ProtoNet	Search...
Other
NextBio	972204.

Entry information

Entry name

COQ8_YEAST

Accession

Primary (citable) accession number: P27697
Secondary accession number(s): D6VU29

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	December 14, 2011
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program