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Protein

Alkaline protease

Gene
N/A
Organism
Bacillus alcalophilus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi113 – 1131Calcium 1
Active sitei143 – 1431Charge relay system
Metal bindingi151 – 1511Calcium 1
Active sitei173 – 1731Charge relay system
Metal bindingi184 – 1841Calcium 1; via carbonyl oxygen
Metal bindingi186 – 1861Calcium 1
Metal bindingi188 – 1881Calcium 1; via carbonyl oxygen
Metal bindingi190 – 1901Calcium 1; via carbonyl oxygen
Metal bindingi274 – 2741Calcium 2; via carbonyl oxygen
Metal bindingi276 – 2761Calcium 2; via carbonyl oxygen
Metal bindingi279 – 2791Calcium 2; via carbonyl oxygen
Active sitei326 – 3261Charge relay system

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

SABIO-RKP27693.

Protein family/group databases

MEROPSiS08.038.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease (EC:3.4.21.-)
OrganismiBacillus alcalophilus
Taxonomic identifieri1445 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2727Sequence analysisAdd
BLAST
Propeptidei28 – 11285PRO_0000027008Add
BLAST
Chaini113 – 380268Alkaline proteasePRO_0000027009Add
BLAST

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1
380
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi117 – 1204Combined sources
Turni121 – 1233Combined sources
Helixi125 – 1306Combined sources
Beta strandi138 – 1447Combined sources
Beta strandi170 – 1723Combined sources
Helixi173 – 18210Combined sources
Beta strandi186 – 1894Combined sources
Beta strandi195 – 2017Combined sources
Helixi213 – 22614Combined sources
Beta strandi229 – 2335Combined sources
Helixi242 – 25211Combined sources
Turni253 – 2553Combined sources
Turni272 – 2754Combined sources
Beta strandi276 – 2794Combined sources
Beta strandi282 – 29110Combined sources
Beta strandi303 – 3064Combined sources
Beta strandi310 – 3145Combined sources
Turni315 – 3173Combined sources
Beta strandi318 – 3225Combined sources
Helixi325 – 34218Combined sources
Helixi348 – 35811Combined sources
Beta strandi364 – 3707Combined sources
Helixi375 – 3795Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AH2NMR-A112-380[»]
ProteinModelPortaliP27693.
SMRiP27693. Positions 112-380.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini138 – 379242Peptidase S8Add
BLAST

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLGKIVA STALLISVAF SSSIASAAEE AKEKYLIGFN EQEAVSEFVE
60 70 80 90 100
QVEANDEVAI LSEEEEVEIE LLHEFETIPV LSVELSPEDV DALELDPAIS
110 120 130 140 150
YIEEDAEVTT MAQSVPWGIS RVQAPAAHNR GLTGSGVKVA VLDTGISTHP
160 170 180 190 200
DLNIRGGASF VPGEPSTQDG NGHGTHVAGT IAALNNSIGV LGVAPNAELY
210 220 230 240 250
AVKVLGASGS GSVSSIAQGL EWAGNNGMHV ANLSLGSPSP SATLEQAVNS
260 270 280 290 300
ATSRGVLVVA ASGNSGAGSI SYPARYANAM AVGATDQNNN RASFSQYGAG
310 320 330 340 350
LDIVAPGVNV QSTYPGSTYA SLNGTSMATP HVAGAAALVK QKNPSWSNVQ
360 370 380
IRNHLKNTAT SLGSTNLYGS GLVNAEAATR
Length:380
Mass (Da):38,853
Last modified:August 1, 1992 - v1
Checksum:i539EA72771B6682C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65086 Genomic DNA. Translation: AAA22212.1.
PIRiA49778.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65086 Genomic DNA. Translation: AAA22212.1.
PIRiA49778.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1AH2NMR-A112-380[»]
ProteinModelPortaliP27693.
SMRiP27693. Positions 112-380.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS08.038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP27693.

Miscellaneous databases

EvolutionaryTraceiP27693.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, characterization, and multiple chromosomal integration of a Bacillus alkaline protease gene."
    van der Laan J.C., Gerritse G., Mulleners L.J.M., van der Hoek R.A., Quax W.J.
    Appl. Environ. Microbiol. 57:901-909(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: PB92.
  2. "Crystal structure of the high-alkaline serine protease PB92 from Bacillus alcalophilus."
    van der Laan J.C., Teplyakov A.V., Kelders H., Kalk K.H., Misset O., Mulleners L.J.M., Dijkstra B.W.
    Protein Eng. 5:405-411(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS).
    Strain: PB92.
  3. "X-ray structure determination and comparison of two crystal forms of a variant (Asn115Arg) of the alkaline protease from Bacillus alcalophilus refined at 1.85-A resolution."
    Sobek H., Hecht H.-J., Aehle W., Schomburg D.
    J. Mol. Biol. 228:108-117(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
  4. "The solution structure of serine protease PB92 from Bacillus alcalophilus presents a rigid fold with a flexible substrate-binding site."
    Martin J.R., Mulder F.A., Karimi-Nejad Y., van der Zwan J., Mariani M., Schipper D., Boelens R.
    Structure 5:521-532(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 112-380.
    Strain: PB92.

Entry informationi

Entry nameiELYA_BACAO
AccessioniPrimary (citable) accession number: P27693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.