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Protein

Alkaline protease

Gene
N/A
Organism
Bacillus alcalophilus
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Cofactori

Ca2+Note: Binds 2 calcium ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi113Calcium 11
Active sitei143Charge relay system1
Metal bindingi151Calcium 11
Active sitei173Charge relay system1
Metal bindingi184Calcium 1; via carbonyl oxygen1
Metal bindingi186Calcium 11
Metal bindingi188Calcium 1; via carbonyl oxygen1
Metal bindingi190Calcium 1; via carbonyl oxygen1
Metal bindingi274Calcium 2; via carbonyl oxygen1
Metal bindingi276Calcium 2; via carbonyl oxygen1
Metal bindingi279Calcium 2; via carbonyl oxygen1
Active sitei326Charge relay system1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

SABIO-RKP27693.

Protein family/group databases

MEROPSiS08.038.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline protease (EC:3.4.21.-)
OrganismiBacillus alcalophilus
Taxonomic identifieri1445 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 27Sequence analysisAdd BLAST27
PropeptideiPRO_000002700828 – 112Add BLAST85
ChainiPRO_0000027009113 – 380Alkaline proteaseAdd BLAST268

Keywords - PTMi

Zymogen

Structurei

Secondary structure

1380
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi117 – 120Combined sources4
Turni121 – 123Combined sources3
Helixi125 – 130Combined sources6
Beta strandi138 – 144Combined sources7
Beta strandi170 – 172Combined sources3
Helixi173 – 182Combined sources10
Beta strandi186 – 189Combined sources4
Beta strandi195 – 201Combined sources7
Helixi213 – 226Combined sources14
Beta strandi229 – 233Combined sources5
Helixi242 – 252Combined sources11
Turni253 – 255Combined sources3
Turni272 – 275Combined sources4
Beta strandi276 – 279Combined sources4
Beta strandi282 – 291Combined sources10
Beta strandi303 – 306Combined sources4
Beta strandi310 – 314Combined sources5
Turni315 – 317Combined sources3
Beta strandi318 – 322Combined sources5
Helixi325 – 342Combined sources18
Helixi348 – 358Combined sources11
Beta strandi364 – 370Combined sources7
Helixi375 – 379Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH2NMR-A112-380[»]
ProteinModelPortaliP27693.
SMRiP27693.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27693.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini138 – 379Peptidase S8Add BLAST242

Sequence similaritiesi

Belongs to the peptidase S8 family.Curated
Contains 1 peptidase S8 domain.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27693-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKPLGKIVA STALLISVAF SSSIASAAEE AKEKYLIGFN EQEAVSEFVE
60 70 80 90 100
QVEANDEVAI LSEEEEVEIE LLHEFETIPV LSVELSPEDV DALELDPAIS
110 120 130 140 150
YIEEDAEVTT MAQSVPWGIS RVQAPAAHNR GLTGSGVKVA VLDTGISTHP
160 170 180 190 200
DLNIRGGASF VPGEPSTQDG NGHGTHVAGT IAALNNSIGV LGVAPNAELY
210 220 230 240 250
AVKVLGASGS GSVSSIAQGL EWAGNNGMHV ANLSLGSPSP SATLEQAVNS
260 270 280 290 300
ATSRGVLVVA ASGNSGAGSI SYPARYANAM AVGATDQNNN RASFSQYGAG
310 320 330 340 350
LDIVAPGVNV QSTYPGSTYA SLNGTSMATP HVAGAAALVK QKNPSWSNVQ
360 370 380
IRNHLKNTAT SLGSTNLYGS GLVNAEAATR
Length:380
Mass (Da):38,853
Last modified:August 1, 1992 - v1
Checksum:i539EA72771B6682C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65086 Genomic DNA. Translation: AAA22212.1.
PIRiA49778.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65086 Genomic DNA. Translation: AAA22212.1.
PIRiA49778.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH2NMR-A112-380[»]
ProteinModelPortaliP27693.
SMRiP27693.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS08.038.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

SABIO-RKP27693.

Miscellaneous databases

EvolutionaryTraceiP27693.

Family and domain databases

Gene3Di3.30.70.80. 1 hit.
3.40.50.200. 1 hit.
InterProiIPR000209. Peptidase_S8/S53_dom.
IPR023827. Peptidase_S8_Asp-AS.
IPR022398. Peptidase_S8_His-AS.
IPR023828. Peptidase_S8_Ser-AS.
IPR015500. Peptidase_S8_subtilisin-rel.
IPR009020. Propept_inh.
IPR010259. S8pro/Inhibitor_I9.
[Graphical view]
PANTHERiPTHR10795. PTHR10795. 1 hit.
PfamiPF05922. Inhibitor_I9. 1 hit.
PF00082. Peptidase_S8. 1 hit.
[Graphical view]
PRINTSiPR00723. SUBTILISIN.
SUPFAMiSSF52743. SSF52743. 1 hit.
SSF54897. SSF54897. 1 hit.
PROSITEiPS00136. SUBTILASE_ASP. 1 hit.
PS00137. SUBTILASE_HIS. 1 hit.
PS00138. SUBTILASE_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiELYA_BACAO
AccessioniPrimary (citable) accession number: P27693
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.