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Protein

Ubiquinone biosynthesis O-methyltransferase, mitochondrial

Gene

COQ3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway. Catalyzes the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate (DHHB) to 3-methoxy-4-hydroxy-5-hexaprenylbenzoate (HMHB) and the methylation of 2-hexaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol (3-demethylubiquinol-6) to ubiquinol-6.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 3,4-dihydroxy-5-all-trans-polyprenylbenzoate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate.UniRule annotation1 Publication
S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n.UniRule annotation1 Publication

Enzyme regulationi

Regulated in response to catabolite repression.1 Publication

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681S-adenosyl-L-methionineUniRule annotation
Binding sitei130 – 1301S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation
Binding sitei153 – 1531S-adenosyl-L-methionineUniRule annotation
Binding sitei196 – 1961S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation

GO - Molecular functioni

  • 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Source: InterPro
  • 3-demethylubiquinone-6 3-O-methyltransferase activity Source: SGD
  • 3-demethylubiquinone-9 3-O-methyltransferase activity Source: UniProtKB-EC
  • hexaprenyldihydroxybenzoate methyltransferase activity Source: SGD

GO - Biological processi

  • ubiquinone biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Ubiquinone biosynthesis

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-13689.
YEAST:YOL096C-MONOMER.
BRENDAi2.1.1.114. 984.
2.1.1.222. 984.
2.1.1.64. 984.
ReactomeiR-SCE-2142789. Ubiquinol biosynthesis.
UniPathwayiUPA00232.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone biosynthesis O-methyltransferase, mitochondrialUniRule annotationCurated
Alternative name(s):
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase1 PublicationUniRule annotation
Short name:
DHHB methyltransferase1 PublicationUniRule annotation
Short name:
DHHB-MTUniRule annotation
Short name:
DHHB-MTaseUniRule annotation
3-demethylubiquinol 3-O-methyltransferaseUniRule annotation1 Publication (EC:2.1.1.64UniRule annotation1 Publication)
3-demethylubiquinone-6 3-O-methyltransferaseUniRule annotation
Hexaprenyldihydroxybenzoate methyltransferaseUniRule annotation
Polyprenyldihydroxybenzoate methyltransferaseUniRule annotation1 Publication (EC:2.1.1.114UniRule annotation1 Publication)
Gene namesi
Name:COQ31 PublicationUniRule annotation
Ordered Locus Names:YOL096CImported
ORF Names:HRF316
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL096C.
SGDiS000005456. COQ3.

Subcellular locationi

GO - Cellular componenti

  • extrinsic component of membrane Source: SGD
  • extrinsic component of mitochondrial inner membrane Source: WormBase
  • mitochondrial inner membrane Source: SGD
  • mitochondrion Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 3232MitochondrionSequence analysisAdd
BLAST
Chaini33 – 312280Ubiquinone biosynthesis O-methyltransferase, mitochondrialPRO_0000035932Add
BLAST

Proteomic databases

MaxQBiP27680.

Interactioni

Subunit structurei

Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 (PubMed:15792955, PubMed:24406904). Interacts directly with COQ4 (PubMed:15792955).2 Publications

Protein-protein interaction databases

BioGridi34306. 63 interactions.
IntActiP27680. 1 interaction.
MINTiMINT-4502029.

Structurei

3D structure databases

ProteinModelPortaliP27680.
SMRiP27680. Positions 40-270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.UniRule annotationCurated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000007284.
HOGENOMiHOG000278064.
InParanoidiP27680.
KOiK00591.
OMAiRKSMCYP.
OrthoDBiEOG092C3VU8.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00472. UbiG. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR010233. UbiG_MeTrfase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01983. UbiG. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRSRFLKV IHVRKQLSAC SRFAIQTQTR CKSTDASEDE VKHFQELAPT
60 70 80 90 100
WWDTDGSQRI LHKMNLTRLD FVQRTVRNQV KIQNPEIFVP GFNYKEFLPE
110 120 130 140 150
YVCDNIQREM QESIETNLDK RPEVSVLDVG CGGGILSESL ARLKWVKNVQ
160 170 180 190 200
GIDLTRDCIM VAKEHAKKDP MLEGKINYEC KALEDVTGQF DIITCMEMLE
210 220 230 240 250
HVDMPSEILR HCWSRLNPEK GILFLSTINR DLISWFTTIF MGENVLKIVP
260 270 280 290 300
KGTHHLSKYI NSKEILAWFN DNYSGQFRLL DLKGTMYLPY QGWVEHDCSD
310
VGNYFMAIQR LN
Length:312
Mass (Da):36,331
Last modified:April 4, 2006 - v2
Checksum:iF0D973ADE4C2D6FD
GO

Sequence cautioni

The sequence AAB63972 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA88165 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99108 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99109 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73270 Genomic DNA. Translation: AAB63972.1. Different initiation.
Z48149 Genomic DNA. Translation: CAA88165.1. Different initiation.
Z74838 Genomic DNA. Translation: CAA99109.1. Different initiation.
Z74837 Genomic DNA. Translation: CAA99108.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10688.1.
PIRiA41171.
RefSeqiNP_014545.2. NM_001183350.1.

Genome annotation databases

EnsemblFungiiYOL096C; YOL096C; YOL096C.
GeneIDi854057.
KEGGisce:YOL096C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73270 Genomic DNA. Translation: AAB63972.1. Different initiation.
Z48149 Genomic DNA. Translation: CAA88165.1. Different initiation.
Z74838 Genomic DNA. Translation: CAA99109.1. Different initiation.
Z74837 Genomic DNA. Translation: CAA99108.1. Different initiation.
BK006948 Genomic DNA. Translation: DAA10688.1.
PIRiA41171.
RefSeqiNP_014545.2. NM_001183350.1.

3D structure databases

ProteinModelPortaliP27680.
SMRiP27680. Positions 40-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi34306. 63 interactions.
IntActiP27680. 1 interaction.
MINTiMINT-4502029.

Proteomic databases

MaxQBiP27680.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYOL096C; YOL096C; YOL096C.
GeneIDi854057.
KEGGisce:YOL096C.

Organism-specific databases

EuPathDBiFungiDB:YOL096C.
SGDiS000005456. COQ3.

Phylogenomic databases

GeneTreeiENSGT00390000007284.
HOGENOMiHOG000278064.
InParanoidiP27680.
KOiK00591.
OMAiRKSMCYP.
OrthoDBiEOG092C3VU8.

Enzyme and pathway databases

UniPathwayiUPA00232.
BioCyciMetaCyc:MONOMER-13689.
YEAST:YOL096C-MONOMER.
BRENDAi2.1.1.114. 984.
2.1.1.222. 984.
2.1.1.64. 984.
ReactomeiR-SCE-2142789. Ubiquinol biosynthesis.

Miscellaneous databases

PROiP27680.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_00472. UbiG. 1 hit.
InterProiIPR029063. SAM-dependent_MTases.
IPR010233. UbiG_MeTrfase.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR01983. UbiG. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCOQ3_YEAST
AccessioniPrimary (citable) accession number: P27680
Secondary accession number(s): D6W1X2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 4, 2006
Last modified: September 7, 2016
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.