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Protein

Ubiquinone biosynthesis O-methyltransferase, mitochondrial

Gene

COQ3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

O-methyltransferase that catalyzes the 2 O-methylation steps in the ubiquinone biosynthetic pathway. Catalyzes the methylation of 3,4-dihydroxy-5-hexaprenylbenzoate (DHHB) to 3-methoxy-4-hydroxy-5-hexaprenylbenzoate (HMHB) and the methylation of 2-hexaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinol (3-demethylubiquinol-6) to ubiquinol-6.1 Publication

Miscellaneous

Present with 259 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + 3,4-dihydroxy-5-all-trans-polyprenylbenzoate = S-adenosyl-L-homocysteine + 3-methoxy-4-hydroxy-5-all-trans-polyprenylbenzoate.UniRule annotation1 Publication
S-adenosyl-L-methionine + 3-demethylubiquinone-n = S-adenosyl-L-homocysteine + ubiquinone-n.UniRule annotation1 Publication

Enzyme regulationi

Regulated in response to catabolite repression.1 Publication

Pathwayi: ubiquinone biosynthesis

This protein is involved in the pathway ubiquinone biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation1 Publication
View all proteins of this organism that are known to be involved in the pathway ubiquinone biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68S-adenosyl-L-methionineUniRule annotation1
Binding sitei130S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei153S-adenosyl-L-methionineUniRule annotation1
Binding sitei196S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1

GO - Molecular functioni

  • 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity Source: InterPro
  • 3-demethylubiquinone-6 3-O-methyltransferase activity Source: SGD
  • 3-demethylubiquinone-9 3-O-methyltransferase activity Source: UniProtKB-EC
  • hexaprenyldihydroxybenzoate methyltransferase activity Source: SGD

GO - Biological processi

  • ubiquinone biosynthetic process Source: SGD

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processUbiquinone biosynthesis
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:YOL096C-MONOMER
YEAST:YOL096C-MONOMER
BRENDAi2.1.1.114 984
2.1.1.222 984
2.1.1.64 984
ReactomeiR-SCE-2142789 Ubiquinol biosynthesis
UniPathwayiUPA00232

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquinone biosynthesis O-methyltransferase, mitochondrialUniRule annotationCurated
Alternative name(s):
3,4-dihydroxy-5-hexaprenylbenzoate methyltransferase1 PublicationUniRule annotation
Short name:
DHHB methyltransferase1 PublicationUniRule annotation
Short name:
DHHB-MTUniRule annotation
Short name:
DHHB-MTaseUniRule annotation
3-demethylubiquinol 3-O-methyltransferaseUniRule annotation1 Publication (EC:2.1.1.64UniRule annotation1 Publication)
3-demethylubiquinone-6 3-O-methyltransferaseUniRule annotation
Hexaprenyldihydroxybenzoate methyltransferaseUniRule annotation
Polyprenyldihydroxybenzoate methyltransferaseUniRule annotation1 Publication (EC:2.1.1.114UniRule annotation1 Publication)
Gene namesi
Name:COQ31 PublicationUniRule annotation
Ordered Locus Names:YOL096CImported
ORF Names:HRF316
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XV

Organism-specific databases

EuPathDBiFungiDB:YOL096C
SGDiS000005456 COQ3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 32MitochondrionUniRule annotationAdd BLAST32
ChainiPRO_000003593233 – 312Ubiquinone biosynthesis O-methyltransferase, mitochondrialAdd BLAST280

Proteomic databases

MaxQBiP27680
PaxDbiP27680
PRIDEiP27680

Interactioni

Subunit structurei

Component of a multi-subunit COQ enzyme complex, composed of at least COQ3, COQ4, COQ5, COQ6, COQ7 and COQ9 (PubMed:15792955, PubMed:24406904). Interacts directly with COQ4 (PubMed:15792955).2 Publications

Protein-protein interaction databases

BioGridi3430665 interactors.
IntActiP27680 5 interactors.
STRINGi4932.YOL096C

Structurei

3D structure databases

ProteinModelPortaliP27680
SMRiP27680
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. UbiG/COQ3 family.UniRule annotationCurated

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000007284
HOGENOMiHOG000278064
InParanoidiP27680
KOiK00591
OMAiGTHDWEK
OrthoDBiEOG092C3VU8

Family and domain databases

HAMAPiMF_00472 UbiG, 1 hit
InterProiView protein in InterPro
IPR029063 SAM-dependent_MTases
IPR010233 UbiG_MeTrfase
SUPFAMiSSF53335 SSF53335, 1 hit
TIGRFAMsiTIGR01983 UbiG, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27680-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLRSRFLKV IHVRKQLSAC SRFAIQTQTR CKSTDASEDE VKHFQELAPT
60 70 80 90 100
WWDTDGSQRI LHKMNLTRLD FVQRTVRNQV KIQNPEIFVP GFNYKEFLPE
110 120 130 140 150
YVCDNIQREM QESIETNLDK RPEVSVLDVG CGGGILSESL ARLKWVKNVQ
160 170 180 190 200
GIDLTRDCIM VAKEHAKKDP MLEGKINYEC KALEDVTGQF DIITCMEMLE
210 220 230 240 250
HVDMPSEILR HCWSRLNPEK GILFLSTINR DLISWFTTIF MGENVLKIVP
260 270 280 290 300
KGTHHLSKYI NSKEILAWFN DNYSGQFRLL DLKGTMYLPY QGWVEHDCSD
310
VGNYFMAIQR LN
Length:312
Mass (Da):36,331
Last modified:April 4, 2006 - v2
Checksum:iF0D973ADE4C2D6FD
GO

Sequence cautioni

The sequence AAB63972 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA88165 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99108 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated
The sequence CAA99109 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73270 Genomic DNA Translation: AAB63972.1 Different initiation.
Z48149 Genomic DNA Translation: CAA88165.1 Different initiation.
Z74838 Genomic DNA Translation: CAA99109.1 Different initiation.
Z74837 Genomic DNA Translation: CAA99108.1 Different initiation.
BK006948 Genomic DNA Translation: DAA10688.1
PIRiA41171
RefSeqiNP_014545.2, NM_001183350.1

Genome annotation databases

EnsemblFungiiYOL096C; YOL096C; YOL096C
GeneIDi854057
KEGGisce:YOL096C

Similar proteinsi

Entry informationi

Entry nameiCOQ3_YEAST
AccessioniPrimary (citable) accession number: P27680
Secondary accession number(s): D6W1X2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: April 4, 2006
Last modified: February 28, 2018
This is version 149 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome