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Protein

Ras-specific guanine nucleotide-releasing factor 1

Gene

Rasgrf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes the exchange of Ras-bound GDP by GTP.1 Publication

GO - Molecular functioni

  • glutamate receptor binding Source: MGI
  • Rho guanyl-nucleotide exchange factor activity Source: InterPro

GO - Biological processi

  • activation of GTPase activity Source: HGNC
  • cell proliferation Source: MGI
  • neuron projection development Source: HGNC
  • positive regulation of GTPase activity Source: HGNC
  • positive regulation of Ras protein signal transduction Source: UniProtKB
  • regulation of neuronal synaptic plasticity Source: MGI
  • regulation of N-methyl-D-aspartate selective glutamate receptor activity Source: MGI
  • regulation of Rac protein signal transduction Source: HGNC
  • regulation of Ras protein signal transduction Source: HGNC
  • regulation of Rho protein signal transduction Source: InterPro
  • regulation of synaptic plasticity Source: UniProtKB
  • small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Enzyme and pathway databases

ReactomeiR-MMU-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-MMU-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-specific guanine nucleotide-releasing factor 1
Short name:
Ras-GRF1
Alternative name(s):
CDC25Mm
Guanine nucleotide-releasing protein
Short name:
GNRP
Ras-specific nucleotide exchange factor CDC25
Gene namesi
Name:Rasgrf1
Synonyms:Cdc25, Grf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:99694. Rasgrf1.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HGNC
  • growth cone Source: HGNC
Complete GO annotation...

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi263 – 2631L → Q: Loss of function and oligomerization. 1 Publication
Mutagenesisi394 – 4007LTLHELL → IIIRDII: Partial loss of function. No effect on oligomerization. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12621262Ras-specific guanine nucleotide-releasing factor 1PRO_0000068881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei71 – 711Phosphoserine; by PLK2By similarity
Modified residuei581 – 5811Phosphoserine; by PLK2By similarity
Modified residuei617 – 6171Phosphoserine; by PLK2By similarity
Modified residuei745 – 7451PhosphoserineCombined sources
Modified residuei766 – 7661Phosphoserine; by PLK2By similarity

Post-translational modificationi

Phosphorylated by PLK2, leading to ubiquitination and degradation by the proteasome.By similarity
Ubiquitinated and degraded following phosphorylation by PLK2.1 Publication
Phosphorylated by SRC and LCK. Phosphorylation by LCK increases its capacity to stimulate the GDP/GTP exchange on Ras, whereas its phosphorylation by SRC seems not to have an effect on stimulation activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP27671.
PaxDbiP27671.
PRIDEiP27671.

PTM databases

iPTMnetiP27671.
PhosphoSiteiP27671.

Expressioni

Tissue specificityi

Brain.

Gene expression databases

BgeeiENSMUSG00000032356.
ExpressionAtlasiP27671. baseline and differential.
GenevisibleiP27671. MM.

Interactioni

Subunit structurei

Homooligomer and heterooligomer with RASGRF2. Interacts with USP8, thereby regulating its stability.2 Publications

GO - Molecular functioni

  • glutamate receptor binding Source: MGI

Protein-protein interaction databases

BioGridi202600. 5 interactions.
DIPiDIP-41194N.
IntActiP27671. 1 interaction.
MINTiMINT-207405.
STRINGi10090.ENSMUSP00000034912.

Structurei

Secondary structure

1
1262
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi1028 – 104417
Helixi1048 – 105710
Helixi1061 – 10644
Helixi1066 – 108722
Helixi1092 – 111120
Helixi1115 – 112511
Helixi1128 – 11314
Helixi1134 – 11385
Helixi1142 – 115413
Helixi1158 – 116912
Helixi1179 – 119214
Helixi1204 – 122219
Helixi1231 – 12388
Helixi1246 – 125611

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IJEX-ray2.20S1028-1262[»]
ProteinModelPortaliP27671.
SMRiP27671. Positions 25-126, 243-612, 942-1261.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27671.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini22 – 130109PH 1PROSITE-ProRule annotationAdd
BLAST
Domaini208 – 23326IQPROSITE-ProRule annotationAdd
BLAST
Domaini244 – 430187DHPROSITE-ProRule annotationAdd
BLAST
Domaini460 – 588129PH 2PROSITE-ProRule annotationAdd
BLAST
Domaini635 – 749115N-terminal Ras-GEFPROSITE-ProRule annotationAdd
BLAST
Domaini1027 – 1259233Ras-GEFPROSITE-ProRule annotationAdd
BLAST

Domaini

The DH (DBL-homology) domain mediates interaction with RASGRF2.

Sequence similaritiesi

Contains 1 DH (DBL-homology) domain.PROSITE-ProRule annotation
Contains 1 IQ domain.PROSITE-ProRule annotation
Contains 1 N-terminal Ras-GEF domain.PROSITE-ProRule annotation
Contains 2 PH domains.PROSITE-ProRule annotation
Contains 1 Ras-GEF domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiP27671.
KOiK04349.
OMAiDNDQNQS.
OrthoDBiEOG091G09V6.
PhylomeDBiP27671.
TreeFamiTF317296.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 3 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27671-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQKAIRLNDG HVVTLGLLAQ KDGTRKGYLS KRSADNPKWQ TKWFALLQNL
60 70 80 90 100
LFYFESDSSP RPSGLYLLEG SICKRAPSPK RGTSSKESGE KQQHYFTVNF
110 120 130 140 150
SNDSQKTLEL RTEDAKDCDE WVAAIARASY KILATEHEAL MQKYLHLLQV
160 170 180 190 200
VETEKTVAKQ LRQQLEDGEV EIERLKTEVT ITNLIKDNDR IQSSNKAGSA
210 220 230 240 250
DDEDSDIKKI KKVQSFLRGW LCRRKWKNII QDYIRSPHAD SMRKRNQVVF
260 270 280 290 300
SMLEAEAEYV QQLHILVNNF LRPLRMAASS KKPPITHDDV SSIFLNSETI
310 320 330 340 350
MFLHQIFYQG LKARISSWPT LVLADLFDIL LPMLNIYQEF VRNHQYSLQI
360 370 380 390 400
LAHCKQNRDF DKLLKQYEAK PDCEERTLET FLTYPMFQIP RYILTLHELL
410 420 430 440 450
AHTPHEHVER NSLDYAKSKL EELSRIMHDE VSETENIRKN LAIERMITEG
460 470 480 490 500
CEILLDTSQT FVRQGSLMQM SLSEKSKSSR GRLGSLSTKK EGERQCFLFS
510 520 530 540 550
KHLIICTRGS GGKLHLTKNG VISLIDCTLL DEPENLDDEA KGAGPEIEHL
560 570 580 590 600
EFKIGVEPKD SLPFTVILVA STRQEKAAWT SDIIQCVDNI RCNGLMMNAF
610 620 630 640 650
EENSKVTVPQ MIKSDASLYC DDVDIRFSKT MNSCKVLQIR YASVERLLER
660 670 680 690 700
LTDLRFLSID FLNTFLHSYR VFTNAMVVLD KLINIYRKPM SAIPARSLEL
710 720 730 740 750
LFSSSHNAKL LYGDAPKSPR ASRKFSSPPP LAIGTSSPSR RRKLSLNIPI
760 770 780 790 800
ITGGKALELA SLGCSSDSYA NIHSPISPFG KTTLDTGKLC MASSLPKTPE
810 820 830 840 850
EIDVPATIPE KPGELSASRK HSSDVLKEES EDDQNHSDED NTEVSPVKSP
860 870 880 890 900
PTPKSFLNRT ITEFPFFNYN NGILMTTCRD LVDNNRSTLS ATSAFAIATA
910 920 930 940 950
GANEGPSNKE VFRRMSLANT GFSSDQRNID KEFVIRRAAT NRVLNVLRHW
960 970 980 990 1000
VTKHTQDFDT DDTLKYRVIC FLEEVMHDPD LLTQERKAAA NIIRTLTLEE
1010 1020 1030 1040 1050
TTEQHSMLEE VILMTEGVKT EPFENHPALE IAEQLTLLDH LVFKSIPYEE
1060 1070 1080 1090 1100
FFGQGWMKAE KYERTPYIMK TTKHFNHVSN FIASEIIRNE DISARASAIE
1110 1120 1130 1140 1150
KWVAVADICR CLHNYNAVLE ITSSINRSAI FRLKKTWLKV SKQTKSLLDK
1160 1170 1180 1190 1200
LQKLVSSDGR FKNLRESLRN CDPPCVPYLG MYLTDLVFIE EGTPNYTEDG
1210 1220 1230 1240 1250
LVNFSKMRMI SHIIREIRQF QQTTYKIDPQ PKVIQYLLDE SFMLDEESLY
1260
ESSLLIEPKL PT
Length:1,262
Mass (Da):144,102
Last modified:October 1, 1996 - v2
Checksum:i38BFE68F7C228DC8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1033 – 10331E → D (PubMed:1379731).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20899 mRNA. Translation: AAA02741.1.
X59868 mRNA. Translation: CAA42525.1.
CCDSiCCDS40722.1.
PIRiS28407.
RefSeqiNP_035375.1. NM_011245.2.
UniGeneiMm.44561.

Genome annotation databases

EnsembliENSMUST00000034912; ENSMUSP00000034912; ENSMUSG00000032356.
GeneIDi19417.
KEGGimmu:19417.
UCSCiuc009qzt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L20899 mRNA. Translation: AAA02741.1.
X59868 mRNA. Translation: CAA42525.1.
CCDSiCCDS40722.1.
PIRiS28407.
RefSeqiNP_035375.1. NM_011245.2.
UniGeneiMm.44561.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2IJEX-ray2.20S1028-1262[»]
ProteinModelPortaliP27671.
SMRiP27671. Positions 25-126, 243-612, 942-1261.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202600. 5 interactions.
DIPiDIP-41194N.
IntActiP27671. 1 interaction.
MINTiMINT-207405.
STRINGi10090.ENSMUSP00000034912.

PTM databases

iPTMnetiP27671.
PhosphoSiteiP27671.

Proteomic databases

MaxQBiP27671.
PaxDbiP27671.
PRIDEiP27671.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034912; ENSMUSP00000034912; ENSMUSG00000032356.
GeneIDi19417.
KEGGimmu:19417.
UCSCiuc009qzt.1. mouse.

Organism-specific databases

CTDi5923.
MGIiMGI:99694. Rasgrf1.

Phylogenomic databases

eggNOGiENOG410IQ6Q. Eukaryota.
ENOG410XPWA. LUCA.
GeneTreeiENSGT00810000125349.
HOGENOMiHOG000046000.
HOVERGENiHBG005208.
InParanoidiP27671.
KOiK04349.
OMAiDNDQNQS.
OrthoDBiEOG091G09V6.
PhylomeDBiP27671.
TreeFamiTF317296.

Enzyme and pathway databases

ReactomeiR-MMU-442982. Ras activation uopn Ca2+ infux through NMDA receptor.
R-MMU-5673001. RAF/MAP kinase cascade.

Miscellaneous databases

ChiTaRSiRasgrf1. mouse.
EvolutionaryTraceiP27671.
PROiP27671.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032356.
ExpressionAtlasiP27671. baseline and differential.
GenevisibleiP27671. MM.

Family and domain databases

Gene3Di1.10.840.10. 1 hit.
1.20.900.10. 1 hit.
2.30.29.30. 2 hits.
InterProiIPR000219. DH-domain.
IPR001331. GDS_CDC24_CS.
IPR000048. IQ_motif_EF-hand-BS.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR000651. Ras-like_Gua-exchang_fac_N.
IPR019804. Ras_G-nucl-exch_fac_CS.
IPR023578. Ras_GEF_dom.
IPR001895. RASGEF_cat_dom.
IPR030745. RasGRF1.
[Graphical view]
PANTHERiPTHR23113:SF193. PTHR23113:SF193. 3 hits.
PfamiPF00169. PH. 2 hits.
PF00617. RasGEF. 1 hit.
PF00618. RasGEF_N. 1 hit.
PF00621. RhoGEF. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 2 hits.
SM00147. RasGEF. 1 hit.
SM00229. RasGEFN. 2 hits.
SM00325. RhoGEF. 1 hit.
[Graphical view]
SUPFAMiSSF48065. SSF48065. 1 hit.
SSF48366. SSF48366. 2 hits.
SSF50729. SSF50729. 2 hits.
PROSITEiPS00741. DH_1. 1 hit.
PS50010. DH_2. 1 hit.
PS50096. IQ. 1 hit.
PS50003. PH_DOMAIN. 2 hits.
PS00720. RASGEF. 1 hit.
PS50009. RASGEF_CAT. 1 hit.
PS50212. RASGEF_NTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRGRF1_MOUSE
AccessioniPrimary (citable) accession number: P27671
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.