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Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731NucleophileBy similarity
Active sitei329 – 3291Proton donorBy similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLB
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Chaini33 – 348316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLBPRO_0000011873Add
BLAST
Propeptidei349 – 37022Removed in mature formBy similarityPRO_0000011874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acidCurated
Glycosylationi361 – 3611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By ethylene and salicylic acid.

Structurei

3D structure databases

ProteinModelPortaliP27666.
SMRiP27666. Positions 35-347.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27666-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTSDKHNTP QMAAITLLGL LLVASTIEIA GAQSIGVCYG MLGNNLPNHW
60 70 80 90 100
EVIQLYKSRN IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM
110 120 130 140 150
EHARWWVQKN VKDFWPDVKI KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY
160 170 180 190 200
KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS QGSFRNDARW FTDPIVGFLR
210 220 230 240 250
DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG SRQYRNLFDA
260 270 280 290 300
MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA
310 320 330 340 350
KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS
360 370
GGVWDSSVET NATASLISEM
Length:370
Mass (Da):40,443
Last modified:July 31, 1992 - v1
Checksum:i3758B156EE9C6AC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60403 Genomic DNA. Translation: AAA63540.1.
PIRiB39115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60403 Genomic DNA. Translation: AAA63540.1.
PIRiB39115.

3D structure databases

ProteinModelPortaliP27666.
SMRiP27666. Positions 35-347.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family."
    Sperisen C., Ryals J., Meins F.
    Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiE13F_TOBAC
AccessioniPrimary (citable) accession number: P27666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1992
Last sequence update: July 31, 1992
Last modified: January 6, 2015
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.