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P27666

- E13F_TOBAC

UniProt

P27666 - E13F_TOBAC

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Protein

Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB

Gene
N/A
Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Implicated in the defense of plants against pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei273 – 2731Nucleophile By similarity
Active sitei329 – 3291Proton donor By similarity

GO - Molecular functioni

  1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

GO - Biological processi

  1. carbohydrate metabolic process Source: InterPro
  2. defense response Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLB
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

  1. vacuole Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3232Add
BLAST
Chaini33 – 348316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLBPRO_0000011873Add
BLAST
Propeptidei349 – 37022Removed in mature form By similarityPRO_0000011874Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei33 – 331Pyrrolidone carboxylic acid Inferred
Glycosylationi361 – 3611N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By ethylene and salicylic acid.

Structurei

3D structure databases

ProteinModelPortaliP27666.
SMRiP27666. Positions 35-347.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27666-1 [UniParc]FASTAAdd to Basket

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MSTSDKHNTP QMAAITLLGL LLVASTIEIA GAQSIGVCYG MLGNNLPNHW    50
EVIQLYKSRN IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM 100
EHARWWVQKN VKDFWPDVKI KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY 150
KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS QGSFRNDARW FTDPIVGFLR 200
DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG SRQYRNLFDA 250
MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA 300
KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS 350
GGVWDSSVET NATASLISEM 370
Length:370
Mass (Da):40,443
Last modified:August 1, 1992 - v1
Checksum:i3758B156EE9C6AC7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60403 Genomic DNA. Translation: AAA63540.1.
PIRiB39115.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M60403 Genomic DNA. Translation: AAA63540.1 .
PIRi B39115.

3D structure databases

ProteinModelPortali P27666.
SMRi P27666. Positions 35-347.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

CAZyi GH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 3.20.20.80. 1 hit.
InterProi IPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view ]
Pfami PF00332. Glyco_hydro_17. 1 hit.
[Graphical view ]
SUPFAMi SSF51445. SSF51445. 1 hit.
PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family."
    Sperisen C., Ryals J., Meins F.
    Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiE13F_TOBAC
AccessioniPrimary (citable) accession number: P27666
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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