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P27666 (E13F_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase, basic
Glucanase GLB
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length370 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Implicated in the defense of plants against pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Vacuole.

Tissue specificity

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Induction

By ethylene and salicylic acid.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentVacuole
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Pyrrolidone carboxylic acid
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentvacuole

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3232
Chain33 – 348316Glucan endo-1,3-beta-glucosidase, basic vacuolar isoform GLB
PRO_0000011873
Propeptide349 – 37022Removed in mature form By similarity
PRO_0000011874

Sites

Active site2731Nucleophile By similarity
Active site3291Proton donor By similarity

Amino acid modifications

Modified residue331Pyrrolidone carboxylic acid Probable
Glycosylation3611N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P27666 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 3758B156EE9C6AC7

FASTA37040,443
        10         20         30         40         50         60 
MSTSDKHNTP QMAAITLLGL LLVASTIEIA GAQSIGVCYG MLGNNLPNHW EVIQLYKSRN 

        70         80         90        100        110        120 
IGRLRLYDPN HGALQALKGS NIEVMLGLPN SDVKHIASGM EHARWWVQKN VKDFWPDVKI 

       130        140        150        160        170        180 
KYIAVGNEIS PVTGTSYLTS FLTPAMVNIY KAIGEAGLGN NIKVSTSVDM TLIGNSYPPS 

       190        200        210        220        230        240 
QGSFRNDARW FTDPIVGFLR DTRAPLLVNI YPYFSYSGNP GQISLPYSLF TAPNVVVQDG 

       250        260        270        280        290        300 
SRQYRNLFDA MLDSVYAALE RSGGASVGIV VSESGWPSAG AFGATYDNAA TYLRNLIQHA 

       310        320        330        340        350        360 
KEGSPRKPGP IETYIFAMFD ENNKNPELEK HFGLFSPNKQ PKYNLNFGVS GGVWDSSVET 

       370 
NATASLISEM 

« Hide

References

[1]"Comparison of cloned genes provides evidence for intergenomic exchange of DNA in the evolution of a tobacco glucan endo-1,3-beta-glucosidase gene family."
Sperisen C., Ryals J., Meins F.
Proc. Natl. Acad. Sci. U.S.A. 88:1820-1824(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60403 Genomic DNA. Translation: AAA63540.1.
PIRB39115.

3D structure databases

ProteinModelPortalP27666.
SMRP27666. Positions 35-347.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13F_TOBAC
AccessionPrimary (citable) accession number: P27666
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: February 19, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries