Reviewed, UniProtKB/Swiss-Prot P27664 (PDE6A_MOUSE)

Names and origin

Protein names

Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
      Short name=GMP-PDE alpha
    EC=3.1.4.35

Gene names

Name:	Pde6a
Synonyms:	Mpa, Pdea

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	cGMP
Molecular function	Hydrolase
PTM	Acetylation Lipoprotein Prenylation
Gene Ontology (GO)
Biological process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 859	858	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha		PRO_0000198829
Regions
	Domain	73 – 222	150	GAF 1
	Domain	254 – 431	178	GAF 2
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Lipidation	856	1	S-farnesyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27664-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 14E49D60B2E75B6F	FASTA	859	99,646
	10 20 30 40 50 60 MGEVTAEEVE KFLDSNIGFA KQYYNLHYRG KVISDLLGAK EAAVDFSNYH DVNSVEESEI 70 80 90 100 110 120 IFDLLRDVQE NLQAEKCTFN VMKKLCFLLR ADRVSLFMYR TRNGIAELAT RLFNVHKDAV 130 140 150 160 170 180 LEDCLVMPDS EIVFPLDMGV VGHVAHSKKI ANVPNTEEDE HFCDFVDNLT EYQTKNILAS 190 200 210 220 230 240 PIMNGKDVVA IIMAVNKIDE PHFTKRDEEI LLKYLNFVNL IMKVFHLSYL HNCETRRGQI 250 260 270 280 290 300 LLWSGSKVFE ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 310 320 330 340 350 360 AYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPADHW ALVSGLPPYV AQNGLICNIM 370 380 390 400 410 420 NAPAEDFFEF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV ATFYNRKDGK PFDDMDETLM 430 440 450 460 470 480 ESLTQFLGWS VLNPDTYESM NKLENRKDIF QDIVKYHVKC DNEEIQKILK TREVYGKEPW 490 500 510 520 530 540 ECEEEELAEI LQRELPDAES YEINKFHFSD LPLTELELVK CGIQMYYELR VWDKFHIPQE 550 560 570 580 590 600 ALVRFMYSLS KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 610 620 630 640 650 660 IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL NRRQHEHAIH 670 680 690 700 710 720 MMDIAIIATD LALYFKKRTM FQKIVDQSKT YESTQEWTQY MMLEQTRKEI VMAMMMTACD 730 740 750 760 770 780 LSAITKPWEV QSKVALLVAA EFWEQGDLER TVLQQNPIPM MDRNKADELP KLQVGFIDFV 790 800 810 820 830 840 CTFVYKEFSR FHEEITPMLD GITNNRKEWK ALADEYEAKM KALEEEKQKQ QAAKQAASGN 850 QPGGNPLQGA PASKSCCIQ

References

[1]	"Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene." Baehr W., Champagne M.S., Lee A.K., Pittler S.J. FEBS Lett. 278:107-114(1991) [PubMed: 1847109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X60664 mRNA. Translation: CAA43072.1.
IPI	IPI00880647.
PIR	S13030.
UniGene	Mm.391106
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSMUSG00000024575. Mus musculus. [Contig view]
Organism-specific databases
MGI	MGI:97524. Pde6a.
Phylogenomic databases
HOVERGEN	P27664.
Enzyme and pathway databases
BRENDA	3.1.4.35. 244.
Gene expression databases
ArrayExpress	P27664.
Bgee	P27664.
CleanEx	MM_PDE6A.
GermOnline	ENSMUSG00000024575. Mus musculus.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Met-dep_phosphohydro_HD. IPR002073. PDEase. [Graphical view]
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

PDE6A_MOUSE

Accession

Primary (citable) accession number: P27664

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)