Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

Pde6a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei559Proton donorBy similarity1
Metal bindingi563Divalent metal cation 1By similarity1
Metal bindingi599Divalent metal cation 1By similarity1
Metal bindingi600Divalent metal cation 1By similarity1
Metal bindingi600Divalent metal cation 2By similarity1
Metal bindingi720Divalent metal cation 1By similarity1

GO - Molecular functioni

GO - Biological processi

  • GMP metabolic process Source: MGI
  • photoreceptor cell maintenance Source: MGI
  • regulation of cytosolic calcium ion concentration Source: MGI
  • retina development in camera-type eye Source: MGI
  • signal transduction Source: InterPro
  • visual perception Source: UniProtKB-KW

Keywordsi

Molecular functionHydrolase
Biological processSensory transduction, Vision
LigandcGMP, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
Short name:
GMP-PDE alpha
Gene namesi
Name:Pde6a
Synonyms:Mpa, Pdea
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97524 Pde6a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001988292 – 856Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaAdd BLAST855
PropeptideiPRO_0000396698857 – 859Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylglycineBy similarity1
Modified residuei856Cysteine methyl esterBy similarity1
Lipidationi856S-farnesyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP27664
PaxDbiP27664
PRIDEiP27664

PTM databases

iPTMnetiP27664
PhosphoSitePlusiP27664

Expressioni

Gene expression databases

CleanExiMM_PDE6A

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025468

Structurei

3D structure databases

ProteinModelPortaliP27664
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini73 – 222GAF 1Add BLAST150
Domaini254 – 431GAF 2Add BLAST178
Domaini483 – 816PDEasePROSITE-ProRule annotationAdd BLAST334

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3689 Eukaryota
ENOG410XRI7 LUCA
HOVERGENiHBG053539
InParanoidiP27664

Family and domain databases

CDDicd00077 HDc, 1 hit
Gene3Di1.10.1300.10, 1 hit
3.30.450.40, 2 hits
InterProiView protein in InterPro
IPR003018 GAF
IPR029016 GAF-like_dom_sf
IPR003607 HD/PDEase_dom
IPR032958 PDE6A
IPR023088 PDEase
IPR002073 PDEase_catalytic_dom
IPR036971 PDEase_catalytic_dom_sf
IPR023174 PDEase_CS
PANTHERiPTHR11347:SF115 PTHR11347:SF115, 1 hit
PfamiView protein in Pfam
PF01590 GAF, 2 hits
PF00233 PDEase_I, 1 hit
PRINTSiPR00387 PDIESTERASE1
SMARTiView protein in SMART
SM00065 GAF, 2 hits
SM00471 HDc, 1 hit
PROSITEiView protein in PROSITE
PS00126 PDEASE_I_1, 1 hit
PS51845 PDEASE_I_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27664-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEVTAEEVE KFLDSNIGFA KQYYNFHYRG KVISDLLGAK EAAVDFSNYH
60 70 80 90 100
DVNSVEESEI IFDLLRDVQE NLQAEKCTFN VMKKLCFLLR ADRMSLFMYR
110 120 130 140 150
TRNGIAELAT RLFNVHKDAV LEDCLVMPDS EIVFPLDMGV VGHVAHSKKI
160 170 180 190 200
ANVPNTEEDE HFCDFVDNLT EYQTKNILAS PIMNGKDVVA IIMAVNKIDE
210 220 230 240 250
PHFTKRDEEI LLKYLNFVNL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE
260 270 280 290 300
ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP
310 320 330 340 350
AYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPADHW ALVSGLPTYV
360 370 380 390 400
AQNGLICNIM NAPAEDFFEF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV
410 420 430 440 450
ATFYNRKDGK PFDDMDETLM ESLTQFLGWS VLNPDTYESM NKLENRKDIF
460 470 480 490 500
QDIVKYHVKC DNEEIQKILK TREVYGKEPW ECEEEELAEI LQGELPDAES
510 520 530 540 550
YEINKFHFSD LPLTELELVK CGIQMYYELR VVDKFHIPQE ALVRFMYSLS
560 570 580 590 600
KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD
610 620 630 640 650
IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL
660 670 680 690 700
NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YESTQEWTQY
710 720 730 740 750
MMLEQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER
760 770 780 790 800
TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD
810 820 830 840 850
GITNNRKEWK ALADEYEAKM KALEEEKQKQ QAAKQAASGN QPGGNPLQGA

PASKSCCIQ
Length:859
Mass (Da):99,530
Last modified:July 27, 2011 - v3
Checksum:iE51B8563A1A93526
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti26F → L in CAA43072 (PubMed:1847109).Curated1
Sequence conflicti94M → V in CAA43072 (PubMed:1847109).Curated1
Sequence conflicti348T → P in CAA43072 (PubMed:1847109).Curated1
Sequence conflicti493G → R in CAA43072 (PubMed:1847109).Curated1
Sequence conflicti532V → W in CAA43072 (PubMed:1847109).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X60664 mRNA Translation: CAA43072.1
AC121903 Genomic DNA No translation available.
AC148012 Genomic DNA No translation available.
PIRiS13030
UniGeneiMm.391106

Similar proteinsi

Entry informationi

Entry nameiPDE6A_MOUSE
AccessioniPrimary (citable) accession number: P27664
Secondary accession number(s): E9Q673
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: April 25, 2018
This is version 145 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health