P27664 (PDE6A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Short name=GMP-PDE alpha
EC=3.1.4.35

Gene names

Name:	Pde6a
Synonyms:	Mpa, Pdea

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	859 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	This protein participates in processes of transmission and amplification of the visual signal.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Subunit structure	Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.
Subcellular location	Cell membrane; Lipid-anchor; Cytoplasmic side Potential.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. Contains 2 GAF domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Cellular component	Cell membrane Membrane
Domain	Repeat
Ligand	Metal-binding cGMP
Molecular function	Hydrolase
PTM	Acetylation Lipoprotein Methylation Prenylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	retina development in camera-type eye Inferred from mutant phenotype. Source: MGI signal transduction Inferred from electronic annotation. Source: InterPro visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 856	855	Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha		PRO_0000198829
	Propeptide	857 – 859	3	Removed in mature form By similarity		PRO_0000396698
Regions
	Domain	73 – 222	150	GAF 1
	Domain	254 – 431	178	GAF 2
Sites
	Active site	559	1	Proton donor By similarity
	Metal binding	563	1	Divalent metal cation 1 By similarity
	Metal binding	599	1	Divalent metal cation 1 By similarity
	Metal binding	600	1	Divalent metal cation 1 By similarity
	Metal binding	600	1	Divalent metal cation 2 By similarity
	Metal binding	720	1	Divalent metal cation 1 By similarity
Amino acid modifications
	Modified residue	2	1	N-acetylglycine By similarity
	Modified residue	856	1	Cysteine methyl ester By similarity
	Lipidation	856	1	S-farnesyl cysteine By similarity
Experimental info
	Sequence conflict	26	1	F → L in CAA43072. Ref.1
	Sequence conflict	94	1	M → V in CAA43072. Ref.1
	Sequence conflict	348	1	T → P in CAA43072. Ref.1
	Sequence conflict	493	1	G → R in CAA43072. Ref.1
	Sequence conflict	532	1	V → W in CAA43072. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27664 [UniParc]. Last modified July 27, 2011. Version 3. Checksum: E51B8563A1A93526	FASTA	859	99,530
	10 20 30 40 50 60 MGEVTAEEVE KFLDSNIGFA KQYYNFHYRG KVISDLLGAK EAAVDFSNYH DVNSVEESEI 70 80 90 100 110 120 IFDLLRDVQE NLQAEKCTFN VMKKLCFLLR ADRMSLFMYR TRNGIAELAT RLFNVHKDAV 130 140 150 160 170 180 LEDCLVMPDS EIVFPLDMGV VGHVAHSKKI ANVPNTEEDE HFCDFVDNLT EYQTKNILAS 190 200 210 220 230 240 PIMNGKDVVA IIMAVNKIDE PHFTKRDEEI LLKYLNFVNL IMKVFHLSYL HNCETRRGQI 250 260 270 280 290 300 LLWSGSKVFE ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 310 320 330 340 350 360 AYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPADHW ALVSGLPTYV AQNGLICNIM 370 380 390 400 410 420 NAPAEDFFEF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV ATFYNRKDGK PFDDMDETLM 430 440 450 460 470 480 ESLTQFLGWS VLNPDTYESM NKLENRKDIF QDIVKYHVKC DNEEIQKILK TREVYGKEPW 490 500 510 520 530 540 ECEEEELAEI LQGELPDAES YEINKFHFSD LPLTELELVK CGIQMYYELR VVDKFHIPQE 550 560 570 580 590 600 ALVRFMYSLS KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 610 620 630 640 650 660 IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL NRRQHEHAIH 670 680 690 700 710 720 MMDIAIIATD LALYFKKRTM FQKIVDQSKT YESTQEWTQY MMLEQTRKEI VMAMMMTACD 730 740 750 760 770 780 LSAITKPWEV QSKVALLVAA EFWEQGDLER TVLQQNPIPM MDRNKADELP KLQVGFIDFV 790 800 810 820 830 840 CTFVYKEFSR FHEEITPMLD GITNNRKEWK ALADEYEAKM KALEEEKQKQ QAAKQAASGN 850 QPGGNPLQGA PASKSCCIQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene." Baehr W., Champagne M.S., Lee A.K., Pittler S.J. FEBS Lett. 278:107-114(1991) [PubMed: 1847109] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60664 mRNA. Translation: CAA43072.1. AC121903 Genomic DNA. No translation available. AC148012 Genomic DNA. No translation available.
IPI	IPI00985654.
PIR	S13030.
UniGene	Mm.391106.
3D structure databases
ProteinModelPortal	P27664.
SMR	P27664. Positions 54-223, 484-815.
ModBase	Search...
Protein-protein interaction databases
STRING	P27664.
Proteomic databases
PRIDE	P27664.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000163174; ENSMUSP00000130462; ENSMUSG00000024575.
Organism-specific databases
MGI	MGI:97524. Pde6a.
Phylogenomic databases
eggNOG	roNOG10173.
HOVERGEN	HBG053539.
InParanoid	P27664.
OrthoDB	EOG44TP77.
Gene expression databases
ArrayExpress	P27664.
Bgee	P27664.
CleanEx	MM_PDE6A.
Genevestigator	P27664.
GermOnline	ENSMUSG00000024575. Mus musculus.
Family and domain databases
InterPro	IPR003018. GAF. IPR003607. Metal-dep_PHydrolase_HD_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Gene3D	G3DSA:1.10.1300.10. PDEase_catalytic_dom. 1 hit.
Pfam	PF01590. GAF. 2 hits. PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00065. GAF. 2 hits. SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
SOURCE	Search...

Entry information

Entry name

PDE6A_MOUSE

Accession

Primary (citable) accession number: P27664
Secondary accession number(s): E9Q673

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 27, 2011
Last modified:	November 16, 2011
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program