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P27664

- PDE6A_MOUSE

UniProt

P27664 - PDE6A_MOUSE

Protein

Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha

Gene

Pde6a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 118 (01 Oct 2014)
      Sequence version 3 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    This protein participates in processes of transmission and amplification of the visual signal.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei559 – 5591Proton donorBy similarity
    Metal bindingi563 – 5631Divalent metal cation 1By similarity
    Metal bindingi599 – 5991Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 1By similarity
    Metal bindingi600 – 6001Divalent metal cation 2By similarity
    Metal bindingi720 – 7201Divalent metal cation 1By similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. retina development in camera-type eye Source: MGI
    2. signal transduction Source: InterPro
    3. visual perception Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Sensory transduction, Vision

    Keywords - Ligandi

    cGMP, Metal-binding

    Enzyme and pathway databases

    ReactomeiREACT_221970. Ca2+ pathway.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
    Short name:
    GMP-PDE alpha
    Gene namesi
    Name:Pde6a
    Synonyms:Mpa, Pdea
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:97524. Pde6a.

    Subcellular locationi

    Cell membrane Curated; Lipid-anchor Curated; Cytoplasmic side Curated

    GO - Cellular componenti

    1. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 856855Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alphaPRO_0000198829Add
    BLAST
    Propeptidei857 – 8593Removed in mature formBy similarityPRO_0000396698

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylglycineBy similarity
    Modified residuei856 – 8561Cysteine methyl esterBy similarity
    Lipidationi856 – 8561S-farnesyl cysteineBy similarity

    Keywords - PTMi

    Acetylation, Lipoprotein, Methylation, Prenylation

    Proteomic databases

    MaxQBiP27664.
    PRIDEiP27664.

    PTM databases

    PhosphoSiteiP27664.

    Expressioni

    Gene expression databases

    CleanExiMM_PDE6A.
    GenevestigatoriP27664.

    Interactioni

    Subunit structurei

    Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

    Structurei

    3D structure databases

    ProteinModelPortaliP27664.
    SMRiP27664. Positions 26-817.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini73 – 222150GAF 1Add
    BLAST
    Domaini254 – 431178GAF 2Add
    BLAST

    Sequence similaritiesi

    Contains 2 GAF domains.Curated

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiNOG242608.
    HOVERGENiHBG053539.
    InParanoidiP27664.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProiIPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view]
    SUPFAMiSSF55781. SSF55781. 3 hits.
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27664-1 [UniParc]FASTAAdd to Basket

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    MGEVTAEEVE KFLDSNIGFA KQYYNFHYRG KVISDLLGAK EAAVDFSNYH    50
    DVNSVEESEI IFDLLRDVQE NLQAEKCTFN VMKKLCFLLR ADRMSLFMYR 100
    TRNGIAELAT RLFNVHKDAV LEDCLVMPDS EIVFPLDMGV VGHVAHSKKI 150
    ANVPNTEEDE HFCDFVDNLT EYQTKNILAS PIMNGKDVVA IIMAVNKIDE 200
    PHFTKRDEEI LLKYLNFVNL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE 250
    ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 300
    AYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPADHW ALVSGLPTYV 350
    AQNGLICNIM NAPAEDFFEF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV 400
    ATFYNRKDGK PFDDMDETLM ESLTQFLGWS VLNPDTYESM NKLENRKDIF 450
    QDIVKYHVKC DNEEIQKILK TREVYGKEPW ECEEEELAEI LQGELPDAES 500
    YEINKFHFSD LPLTELELVK CGIQMYYELR VVDKFHIPQE ALVRFMYSLS 550
    KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 600
    IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL 650
    NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YESTQEWTQY 700
    MMLEQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER 750
    TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD 800
    GITNNRKEWK ALADEYEAKM KALEEEKQKQ QAAKQAASGN QPGGNPLQGA 850
    PASKSCCIQ 859
    Length:859
    Mass (Da):99,530
    Last modified:July 27, 2011 - v3
    Checksum:iE51B8563A1A93526
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti26 – 261F → L in CAA43072. (PubMed:1847109)Curated
    Sequence conflicti94 – 941M → V in CAA43072. (PubMed:1847109)Curated
    Sequence conflicti348 – 3481T → P in CAA43072. (PubMed:1847109)Curated
    Sequence conflicti493 – 4931G → R in CAA43072. (PubMed:1847109)Curated
    Sequence conflicti532 – 5321V → W in CAA43072. (PubMed:1847109)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60664 mRNA. Translation: CAA43072.1.
    AC121903 Genomic DNA. No translation available.
    AC148012 Genomic DNA. No translation available.
    PIRiS13030.
    UniGeneiMm.391106.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X60664 mRNA. Translation: CAA43072.1 .
    AC121903 Genomic DNA. No translation available.
    AC148012 Genomic DNA. No translation available.
    PIRi S13030.
    UniGenei Mm.391106.

    3D structure databases

    ProteinModelPortali P27664.
    SMRi P27664. Positions 26-817.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P27664.

    Proteomic databases

    MaxQBi P27664.
    PRIDEi P27664.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:97524. Pde6a.

    Phylogenomic databases

    eggNOGi NOG242608.
    HOVERGENi HBG053539.
    InParanoidi P27664.

    Enzyme and pathway databases

    Reactomei REACT_221970. Ca2+ pathway.

    Miscellaneous databases

    PROi P27664.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_PDE6A.
    Genevestigatori P27664.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    3.30.450.40. 3 hits.
    InterProi IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 2 hits.
    PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00065. GAF. 2 hits.
    SM00471. HDc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55781. SSF55781. 3 hits.
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene."
      Baehr W., Champagne M.S., Lee A.K., Pittler S.J.
      FEBS Lett. 278:107-114(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Retina.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.

    Entry informationi

    Entry nameiPDE6A_MOUSE
    AccessioniPrimary (citable) accession number: P27664
    Secondary accession number(s): E9Q673
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 118 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3