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P27664

- PDE6A_MOUSE

UniProt

P27664 - PDE6A_MOUSE

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Protein
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
Gene
Pde6a, Mpa, Pdea
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

This protein participates in processes of transmission and amplification of the visual signal.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei559 – 5591Proton donor By similarity
Metal bindingi563 – 5631Divalent metal cation 1 By similarity
Metal bindingi599 – 5991Divalent metal cation 1 By similarity
Metal bindingi600 – 6001Divalent metal cation 1 By similarity
Metal bindingi600 – 6001Divalent metal cation 2 By similarity
Metal bindingi720 – 7201Divalent metal cation 1 By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. retina development in camera-type eye Source: MGI
  2. signal transduction Source: InterPro
  3. visual perception Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Sensory transduction, Vision

Keywords - Ligandi

cGMP, Metal-binding

Enzyme and pathway databases

ReactomeiREACT_221970. Ca2+ pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha (EC:3.1.4.35)
Short name:
GMP-PDE alpha
Gene namesi
Name:Pde6a
Synonyms:Mpa, Pdea
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:97524. Pde6a.

Subcellular locationi

GO - Cellular componenti

  1. plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 856855Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha
PRO_0000198829Add
BLAST
Propeptidei857 – 8593Removed in mature form By similarity
PRO_0000396698

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylglycine By similarity
Modified residuei856 – 8561Cysteine methyl ester By similarity
Lipidationi856 – 8561S-farnesyl cysteine By similarity

Keywords - PTMi

Acetylation, Lipoprotein, Methylation, Prenylation

Proteomic databases

MaxQBiP27664.
PRIDEiP27664.

PTM databases

PhosphoSiteiP27664.

Expressioni

Gene expression databases

CleanExiMM_PDE6A.
GenevestigatoriP27664.

Interactioni

Subunit structurei

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Structurei

3D structure databases

ProteinModelPortaliP27664.
SMRiP27664. Positions 26-817.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini73 – 222150GAF 1
Add
BLAST
Domaini254 – 431178GAF 2
Add
BLAST

Sequence similaritiesi

Contains 2 GAF domains.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiNOG242608.
HOVERGENiHBG053539.
InParanoidiP27664.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProiIPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view]
SUPFAMiSSF55781. SSF55781. 3 hits.
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27664-1 [UniParc]FASTAAdd to Basket

« Hide

MGEVTAEEVE KFLDSNIGFA KQYYNFHYRG KVISDLLGAK EAAVDFSNYH    50
DVNSVEESEI IFDLLRDVQE NLQAEKCTFN VMKKLCFLLR ADRMSLFMYR 100
TRNGIAELAT RLFNVHKDAV LEDCLVMPDS EIVFPLDMGV VGHVAHSKKI 150
ANVPNTEEDE HFCDFVDNLT EYQTKNILAS PIMNGKDVVA IIMAVNKIDE 200
PHFTKRDEEI LLKYLNFVNL IMKVFHLSYL HNCETRRGQI LLWSGSKVFE 250
ELTDIERQFH KALYTVRAFL NCDRYSVGLL DMTKQKEFFD VWPVLMGEAP 300
AYSGPRTPDG REINFYKVID YILHGKEDIK VIPNPPADHW ALVSGLPTYV 350
AQNGLICNIM NAPAEDFFEF QKEPLDESGW MIKNVLSMPI VNKKEEIVGV 400
ATFYNRKDGK PFDDMDETLM ESLTQFLGWS VLNPDTYESM NKLENRKDIF 450
QDIVKYHVKC DNEEIQKILK TREVYGKEPW ECEEEELAEI LQGELPDAES 500
YEINKFHFSD LPLTELELVK CGIQMYYELR VVDKFHIPQE ALVRFMYSLS 550
KGYRRITYHN WRHGFNVGQT MFSLLVTGKL KRYFTDLEAL AMVTAAFCHD 600
IDHRGTNNLY QMKSQNPLAK LHGSSILERH HLEFGKTLLR DESLNIFQNL 650
NRRQHEHAIH MMDIAIIATD LALYFKKRTM FQKIVDQSKT YESTQEWTQY 700
MMLEQTRKEI VMAMMMTACD LSAITKPWEV QSKVALLVAA EFWEQGDLER 750
TVLQQNPIPM MDRNKADELP KLQVGFIDFV CTFVYKEFSR FHEEITPMLD 800
GITNNRKEWK ALADEYEAKM KALEEEKQKQ QAAKQAASGN QPGGNPLQGA 850
PASKSCCIQ 859
Length:859
Mass (Da):99,530
Last modified:July 27, 2011 - v3
Checksum:iE51B8563A1A93526
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti26 – 261F → L in CAA43072. 1 Publication
Sequence conflicti94 – 941M → V in CAA43072. 1 Publication
Sequence conflicti348 – 3481T → P in CAA43072. 1 Publication
Sequence conflicti493 – 4931G → R in CAA43072. 1 Publication
Sequence conflicti532 – 5321V → W in CAA43072. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60664 mRNA. Translation: CAA43072.1.
AC121903 Genomic DNA. No translation available.
AC148012 Genomic DNA. No translation available.
PIRiS13030.
UniGeneiMm.391106.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X60664 mRNA. Translation: CAA43072.1 .
AC121903 Genomic DNA. No translation available.
AC148012 Genomic DNA. No translation available.
PIRi S13030.
UniGenei Mm.391106.

3D structure databases

ProteinModelPortali P27664.
SMRi P27664. Positions 26-817.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P27664.

Proteomic databases

MaxQBi P27664.
PRIDEi P27664.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:97524. Pde6a.

Phylogenomic databases

eggNOGi NOG242608.
HOVERGENi HBG053539.
InParanoidi P27664.

Enzyme and pathway databases

Reactomei REACT_221970. Ca2+ pathway.

Miscellaneous databases

PROi P27664.
SOURCEi Search...

Gene expression databases

CleanExi MM_PDE6A.
Genevestigatori P27664.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
3.30.450.40. 3 hits.
InterProi IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF01590. GAF. 2 hits.
PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00065. GAF. 2 hits.
SM00471. HDc. 1 hit.
[Graphical view ]
SUPFAMi SSF55781. SSF55781. 3 hits.
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete cDNA sequences of mouse rod photoreceptor cGMP phosphodiesterase alpha- and beta-subunits, and identification of beta'-, a putative beta-subunit isozyme produced by alternative splicing of the beta-subunit gene."
    Baehr W., Champagne M.S., Lee A.K., Pittler S.J.
    FEBS Lett. 278:107-114(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Retina.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiPDE6A_MOUSE
AccessioniPrimary (citable) accession number: P27664
Secondary accession number(s): E9Q673
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: September 3, 2014
This is version 117 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi