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P27658 (CO8A1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Collagen alpha-1(VIII) chain
Alternative name(s):
Endothelial collagen

Cleaved into the following chain:

  1. Vastatin
Gene names
Name:COL8A1
Synonyms:C3orf7
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length744 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Macromolecular component of the subendothelium. Major component of the Descemet's membrane (basement membrane) of corneal endothelial cells. Also component of the endothelia of blood vessels. Necessary for migration and proliferation of vascular smooth muscle cells and thus, has a potential role in the maintenance of vessel wall integrity and structure, in particular in atherogenesis. Ref.7 Ref.9

Vastatin, the C-terminal fragment comprising the NC1 domain, inhibits aortic endothelial cell proliferation and causes cell apoptosis. Ref.7 Ref.9

Subunit structure

Homotrimers, or heterotrimers in association with alpha 2(VIII) type collagens. Four homotrimers can form a tetrhedron stabilized by central interacting C-terminal NC1 trimers. Ref.10

Subcellular location

Secretedextracellular spaceextracellular matrixbasement membrane.

Tissue specificity

Expressed primarily in the subendothelium of large blood vessels. Also expressed in arterioles and venules in muscle, heart, kidney, spleen, umbilical cord, liver and lung and is also found in connective tissue layers around hair follicles, around nerve bundles in muscle, in the dura of the optic nerve, in cornea and sclera, and in the perichondrium of cartilaginous tissues. In the kidney, expressed in mesangial cells, glomerular endothelial cells, and tubular epithelial cells. Also expressed in mast cells, and in astrocytes during the repair process. Expressed in Descemet's membrane. Specifically expressed in peritoneal fibroblasts and mesothelial cells. Ref.5 Ref.7 Ref.8

Induction

Up-regulated during vascular injury, in atherosclerosis and in diabetes. Ref.11

Post-translational modification

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

Proteolytically cleaved by neutrophil elastase, in vitro. Proteolytic processing produces the C-terminal NC1 domain fragment, vastatin. Ref.6

Miscellaneous

Four consecutive Gly-Pro-Pro triplets are present at the C-terminus of the triple-helical region. These may provide the high thermal stability of this region.

Sequence similarities

Contains 1 C1q domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2727 Potential
Chain28 – 744717Collagen alpha-1(VIII) chain
PRO_0000005762
Chain572 – 744173Vastatin
PRO_0000390484

Regions

Domain611 – 744134C1q
Region29 – 11789Nonhelical region (NC2)
Region118 – 571454Triple-helical region (COL1)
Region572 – 744173Nonhelical region (NC1)

Experimental info

Sequence conflict2621P → L in CAA40748. Ref.1
Sequence conflict2971P → R in CAA40748. Ref.1
Sequence conflict3441P → A in CAA40748. Ref.1
Sequence conflict3821A → S in CAA40748. Ref.1
Sequence conflict3881P → S in CAA40748. Ref.1
Sequence conflict4541L → F in CAA40748. Ref.1
Sequence conflict4641A → H in CAA40748. Ref.1
Sequence conflict6011Y → T in CAA40748. Ref.1
Sequence conflict6311A → G in CAA40748. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P27658 [UniParc].

Last modified July 11, 2002. Version 2.
Checksum: 2BC1B0955DE2C9A3

FASTA74473,364
        10         20         30         40         50         60 
MAVLPGPLQL LGVLLTISLS SIRLIQAGAY YGIKPLPPQI PPQMPPQIPQ YQPLGQQVPH 

        70         80         90        100        110        120 
MPLAKDGLAM GKEMPHLQYG KEYPHLPQYM KEIQPAPRMG KEAVPKKGKE IPLASLRGEQ 

       130        140        150        160        170        180 
GPRGEPGPRG PPGPPGLPGH GIPGIKGKPG PQGYPGVGKP GMPGMPGKPG AMGMPGAKGE 

       190        200        210        220        230        240 
IGQKGEIGPM GIPGPQGPPG PHGLPGIGKP GGPGLPGQPG PKGDRGPKGL PGPQGLRGPK 

       250        260        270        280        290        300 
GDKGFGMPGA PGVKGPPGMH GPPGPVGLPG VGKPGVTGFP GPQGPLGKPG APGEPGPQGP 

       310        320        330        340        350        360 
IGVPGVQGPP GIPGIGKPGQ DGIPGQPGFP GGKGEQGLPG LPGPPGLPGI GKPGFPGPKG 

       370        380        390        400        410        420 
DRGMGGVPGA LGPRGEKGPI GAPGIGGPPG EPGLPGIPGP MGPPGAIGFP GPKGEGGIVG 

       430        440        450        460        470        480 
PQGPPGPKGE PGLQGFPGKP GFLGEVGPPG MRGLPGPIGP KGEAGQKGVP GLPGVPGLLG 

       490        500        510        520        530        540 
PKGEPGIPGD QGLQGPPGIP GIGGPSGPIG PPGIPGPKGE PGLPGPPGFP GIGKPGVAGL 

       550        560        570        580        590        600 
HGPPGKPGAL GPQGQPGLPG PPGPPGPPGP PAVMPPTPPP QGEYLPDMGL GIDGVKPPHA 

       610        620        630        640        650        660 
YGAKKGKNGG PAYEMPAFTA ELTAPFPPVG APVKFNKLLY NGRQNYNPQT GIFTCEVPGV 

       670        680        690        700        710        720 
YYFAYHVHCK GGNVWVALFK NNEPVMYTYD EYKKGFLDQA SGSAVLLLRP GDRVFLQMPS 

       730        740 
EQAAGLYAGQ YVHSSFSGYL LYPM 

« Hide

References

« Hide 'large scale' references
[1]"The complete primary structure of the human alpha 1 (VIII) chain and assignment of its gene (COL8A1) to chromosome 3."
Muragaki Y., Mattei M.-G., Yamaguchi N., Olsen B.R., Ninomiya Y.
Eur. J. Biochem. 197:615-622(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[3]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Lung.
[5]"Distribution of type VIII collagen in tissues: an immunohistochemical study."
Kittelberger R., Davis P.F., Flynn D.W., Greenhill N.S.
Connect. Tissue Res. 24:303-318(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[6]"Cleavage of type VIII collagen by human neutrophil elastase."
Kittelberger R., Neale T.J., Francky K.T., Greenhill N.S., Gibson G.J.
Biochim. Biophys. Acta 1139:295-299(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEOLYTIC PROCESSING.
[7]"Human mast cells produce type VIII collagen in vivo."
Ruger B., Dunbar P.R., Hasan Q., Sawada H., Kittelberger R., Greenhill N., Neale T.J.
Int. J. Exp. Pathol. 75:397-404(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY, POSSIBLE FUNCTION.
[8]"The alpha1(VIII) and alpha2(VIII) collagen chains form two distinct homotrimeric proteins in vivo."
Greenhill N.S., Ruger B.M., Hasan Q., Davis P.F.
Matrix Biol. 19:19-28(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[9]"NC1 domain of human type VIII collagen (alpha 1) inhibits bovine aortic endothelial cell proliferation and causes cell apoptosis."
Xu R., Yao Z.-Y., Xin L., Zhang Q., Li T.-P., Gan R.-B.
Biochem. Biophys. Res. Commun. 289:264-268(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION OF VASTATIN.
[10]"Expression and supramolecular assembly of recombinant alpha1(viii) and alpha2(viii) collagen homotrimers."
Stephan S., Sherratt M.J., Hodson N., Shuttleworth C.A., Kielty C.M.
J. Biol. Chem. 279:21469-21477(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBUNIT.
[11]"Collagen type VIII expression in human diabetic nephropathy."
Gerth J., Cohen C.D., Hopfer U., Lindenmeyer M.T., Sommer M., Grone H.J., Wolf G.
Eur. J. Clin. Invest. 37:767-773(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X57527 mRNA. Translation: CAA40748.1.
BT009917 mRNA. Translation: AAP88919.1.
CH471052 Genomic DNA. Translation: EAW79837.1.
CH471052 Genomic DNA. Translation: EAW79838.1.
CH471052 Genomic DNA. Translation: EAW79840.1.
BC013581 mRNA. Translation: AAH13581.1.
CCDSCCDS2934.1.
PIRS15435.
RefSeqNP_001841.2. NM_001850.4.
NP_065084.2. NM_020351.3.
UniGeneHs.654548.
Hs.740613.
Hs.740617.

3D structure databases

ProteinModelPortalP27658.
SMRP27658. Positions 614-744.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107692. 6 interactions.
IntActP27658. 5 interactions.
MINTMINT-2857732.
STRING9606.ENSP00000261037.

PTM databases

PhosphoSiteP27658.

Polymorphism databases

DMDM21903375.

Proteomic databases

PaxDbP27658.
PRIDEP27658.

Protocols and materials databases

DNASU1295.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000261037; ENSP00000261037; ENSG00000144810.
ENST00000273342; ENSP00000273342; ENSG00000144810.
GeneID1295.
KEGGhsa:1295.
UCSCuc003dtg.2. human.

Organism-specific databases

CTD1295.
GeneCardsGC03P099357.
HGNCHGNC:2215. COL8A1.
HPAHPA053107.
MIM120251. gene.
neXtProtNX_P27658.
PharmGKBPA26731.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG114228.
HOGENOMHOG000085653.
HOVERGENHBG108220.
InParanoidP27658.
OMATCEVPGV.
OrthoDBEOG70ZZPW.
PhylomeDBP27658.
TreeFamTF334029.

Enzyme and pathway databases

ReactomeREACT_118779. Extracellular matrix organization.

Gene expression databases

ArrayExpressP27658.
BgeeP27658.
CleanExHS_COL8A1.
GenevestigatorP27658.

Family and domain databases

Gene3D2.60.120.40. 1 hit.
InterProIPR001073. C1q.
IPR008160. Collagen.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamPF00386. C1q. 1 hit.
PF01391. Collagen. 4 hits.
[Graphical view]
PRINTSPR00007. COMPLEMNTC1Q.
SMARTSM00110. C1Q. 1 hit.
[Graphical view]
SUPFAMSSF49842. SSF49842. 1 hit.
PROSITEPS50871. C1Q. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

GeneWikiCollagen,_type_VIII,_alpha_1.
GenomeRNAi1295.
NextBio5255.
PROP27658.
SOURCESearch...

Entry information

Entry nameCO8A1_HUMAN
AccessionPrimary (citable) accession number: P27658
Secondary accession number(s): D3DN42, Q53XI6, Q96D07
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 11, 2002
Last modified: July 9, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 3

Human chromosome 3: entries, gene names and cross-references to MIM