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Protein

Pancreatic triacylglycerol lipase

Gene

Pnlip

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays an important role in fat metabolism. It preferentially splits the esters of long-chain fatty acids at positions 1 and 3, producing mainly 2-monoacylglycerol and free fatty acids, and shows considerably higher activity against insoluble emulsified substrates than against soluble ones.

Catalytic activityi

Triacylglycerol + H2O = diacylglycerol + a carboxylate.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei169NucleophileBy similarity1
Active sitei193Charge relay systemPROSITE-ProRule annotation1
Metal bindingi204Calcium; via carbonyl oxygenBy similarity1
Metal bindingi207Calcium; via carbonyl oxygenBy similarity1
Metal bindingi209CalciumBy similarity1
Metal bindingi212CalciumBy similarity1
Active sitei280Charge relay systemPROSITE-ProRule annotation1

GO - Molecular functioni

  • lipase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • triglyceride lipase activity Source: RGD

GO - Biological processi

  • lipid catabolic process Source: UniProtKB-KW
  • lipid metabolic process Source: RGD
  • post-embryonic development Source: RGD
  • response to lipid Source: RGD
  • response to peptide hormone Source: RGD

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism
LigandCalcium, Metal-binding

Protein family/group databases

ESTHERiratno-1plip Pancreatic_lipase

Names & Taxonomyi

Protein namesi
Recommended name:
Pancreatic triacylglycerol lipase (EC:3.1.1.3)
Short name:
PL
Short name:
Pancreatic lipase
Gene namesi
Name:Pnlip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi3360 Pnlip

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 161 PublicationAdd BLAST16
ChainiPRO_000001778817 – 465Pancreatic triacylglycerol lipaseAdd BLAST449

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi20 ↔ 26PROSITE-ProRule annotation
Disulfide bondi107 ↔ 118PROSITE-ProRule annotation
Disulfide bondi254 ↔ 278PROSITE-ProRule annotation
Disulfide bondi302 ↔ 313PROSITE-ProRule annotation
Disulfide bondi316 ↔ 321PROSITE-ProRule annotation
Disulfide bondi449 ↔ 465PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP27657
PRIDEiP27657

Expressioni

Tissue specificityi

Pancreatic secretory (zymogen) granule.

Inductioni

By colipase/CLPS in the presence of bile salts.

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000024065

Structurei

3D structure databases

ProteinModelPortaliP27657
SMRiP27657
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini355 – 465PLATPROSITE-ProRule annotationAdd BLAST111

Sequence similaritiesi

Belongs to the AB hydrolase superfamily. Lipase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IHRX Eukaryota
ENOG410Y92X LUCA
HOGENOMiHOG000038552
HOVERGENiHBG003243
InParanoidiP27657
KOiK14073
PhylomeDBiP27657

Family and domain databases

CDDicd00707 Pancreat_lipase_like, 1 hit
Gene3Di3.40.50.1820, 1 hit
InterProiView protein in InterPro
IPR029058 AB_hydrolase
IPR013818 Lipase/vitellogenin
IPR016272 Lipase_LIPH
IPR033906 Lipase_N
IPR002331 Lipase_panc
IPR001024 PLAT/LH2_dom
IPR036392 PLAT/LH2_dom_sf
IPR000734 TAG_lipase
PANTHERiPTHR11610 PTHR11610, 1 hit
PfamiView protein in Pfam
PF00151 Lipase, 1 hit
PF01477 PLAT, 1 hit
PIRSFiPIRSF000865 Lipoprotein_lipase_LIPH, 1 hit
PRINTSiPR00823 PANCLIPASE
PR00821 TAGLIPASE
SMARTiView protein in SMART
SM00308 LH2, 1 hit
SUPFAMiSSF49723 SSF49723, 1 hit
SSF53474 SSF53474, 1 hit
PROSITEiView protein in PROSITE
PS00120 LIPASE_SER, 1 hit
PS50095 PLAT, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27657-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLMLWTFAVL LGAVAGKEVC FDKLGCFSDD APWSGTIDRP LKALPWSPAQ
60 70 80 90 100
INTRFLLYTN ENQDNYQKIT SDASSIRNSN FKTNRKTRII IHGFIDKGEE
110 120 130 140 150
NWLSDMCKNM FKVESVNCIC VDWKGGSRAT YTQATQNVRV VGAEVALLVN
160 170 180 190 200
VLKSDLGHPP DNVHLIGHSL GSHVAGEAGK RTFGAIGRIT GLDAAEPYFQ
210 220 230 240 250
GTPEEVRLDP TDAQFVDAIH TDAAPIIPNL GFGMSQTVGH LDFFPNGGME
260 270 280 290 300
MPGCQKNILS QIVDIDGIWE GTRDFAACNH LRSYKYYTDS IVNPTGFSGF
310 320 330 340 350
SCSSYNVFSA NKCFPCGSEG CPQMGHYADK YPGKTKELYQ KFYLNTGDKS
360 370 380 390 400
NFARWRYQVT VTLSGQKVTG HILVSLFGNG GNSKQYEVFK GSLHPGDTHV
410 420 430 440 450
KEFDSDMDVG DLQKVKFIWY NNVINPTLPK VGASRISVER NDGRVFNFCS
460
QDTVREDVLL TLSAC
Length:465
Mass (Da):51,440
Last modified:August 1, 1992 - v1
Checksum:iCF014D58AA09A6A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58369 mRNA Translation: AAA79888.1
RefSeqiNP_037293.1, NM_013161.2
UniGeneiRn.10130

Genome annotation databases

GeneIDi25702
KEGGirno:25702

Similar proteinsi

Entry informationi

Entry nameiLIPP_RAT
AccessioniPrimary (citable) accession number: P27657
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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