Reviewed, UniProtKB/Swiss-Prot P27656 (LIPC_MOUSE)

Names and origin

Protein names

Recommended name:
    Hepatic triacylglycerol lipase
      Short name=Hepatic lipase
      Short name=HL
    EC=3.1.1.3
Alternative name(s):
    Lipase member C

Gene names

Name:	Lipc
Synonyms:	Hpl

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	510 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.
Catalytic activity	Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Subcellular location	Secreted.
Sequence similarities	Belongs to the AB hydrolase superfamily. Lipase family. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Lipid degradation
Cellular component	HDL Secreted
Domain	Signal
Ligand	Heparin-binding
Molecular function	Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	cholesterol homeostasis Inferred from genetic interaction. Source: MGI cholesterol metabolic process Inferred from genetic interaction. Source: MGI cholesterol transport Inferred from mutant phenotype. Source: MGI
Cellular component	high-density lipoprotein particle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	heparin binding Inferred from electronic annotation. Source: UniProtKB-KW triglyceride lipase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	By similarity
	Chain	23 – 510	488	Hepatic triacylglycerol lipase		PRO_0000017770
Regions
	Domain	353 – 487	135	PLAT
	Region	183 – 194	12	Heparin-binding Potential
Sites
	Active site	169	1	Nucleophile By similarity
	Active site	195	1	Charge relay system By similarity
	Active site	280	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Potential
	Glycosylation	398	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P27656-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: EFE0B919C476BAEC	FASTA	510	57,373
	10 20 30 40 50 60 MGNPLQISIF LVFCIFIQSS ACGQGVGTEP FGRSLGATEA SKPLKKPETR FLLFQDENDR 70 80 90 100 110 120 LGCRLRPQHP ETLQECGFNS SQPLIMIIHG WSVDGLLENW IWKIVSALKS RQSQPVNVGL 130 140 150 160 170 180 VDWISLAYQH YTIAVQNTRI VGQDVAALLL WLEESAKFSR SKVHLIGYSL GAHVSGFAGS 190 200 210 220 230 240 SMDGKNKIGR ITGLDPAGPM FEGTSPNERL SPDDANFVDA IHTFTREHMG LSVGIKQPIA 250 260 270 280 290 300 HYDFYPNGGS FQPGCHFLEL YKHIAEHGLN AITQTIKCAH ERSVHPFIDS LQHSDLQSIG 310 320 330 340 350 360 FQCSDMGSFS QGLCLSCKKG RCNTLGYDIR KDRSGKSKRL FLITRAQSPF KVYHYQFKIQ 370 380 390 400 410 420 FINQIEKPVE PTFTMSLLGT KEEIKRIPIT LGEGITSNKT YSFLITLDKD IGELILLKFK 430 440 450 460 470 480 WENSAVWANV WNTVQTIMLW GIEPHHSGLI LKTIWVKAGE TQQRMTFCPE NLDDLQLHPS 490 500 510 QEKVFVNCEV KSKRLTESKE QMSQETHAKK

References

[1]	"Characterization of cDNA encoding the mouse hepatic triglyceride lipase and expression by in vitro translation." Chang S.F., Netter H.J., Will H. FEBS Lett. 289:69-72(1991) [PubMed: 1840530] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X58426 mRNA. Translation: CAA41329.1.
IPI	IPI00331108.
UniGene	Mm.390187
3D structure databases
HSSP	HSSP built from PDB template 1RP1 based on UniProtKB P06857.
ModBase	Search...
Protein-protein interaction databases
STRING	P27656.
PTM databases
PhosphoSite	P27656.
Proteomic databases
PRIDE	P27656.
Genome annotation databases
Ensembl	ENSMUST00000034731; ENSMUSP00000034731; ENSMUSG00000032207; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:96216. Lipc.
Phylogenomic databases
HOVERGEN	P27656.
Enzyme and pathway databases
BRENDA	3.1.1.3. 244.
Gene expression databases
ArrayExpress	P27656.
Bgee	P27656.
CleanEx	MM_LIPC.
Genevestigator	P27656.
GermOnline	ENSMUSG00000032207. Mus musculus.
Family and domain databases
InterPro	IPR000734. Lipase. IPR002333. Lipase_hep. IPR013818. Lipase_N. IPR008262. Lipase_Ser_AS. IPR001024. LipOase_LH2. IPR016272. Lipoprotein_lipase_LIPH. [Graphical view]
PANTHER	PTHR11610. Lipase. 1 hit. PTHR11610:SF2. Lipase_hep. 1 hit.
Pfam	PF00151. Lipase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PIRSF	PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
PRINTS	PR00824. HEPLIPASE. PR00821. TAGLIPASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00120. LIPASE_SER. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

LIPC_MOUSE

Accession

Primary (citable) accession number: P27656

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	October 13, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)