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P27656

- LIPC_MOUSE

UniProt

P27656 - LIPC_MOUSE

Protein

Hepatic triacylglycerol lipase

Gene

Lipc

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin.

    Catalytic activityi

    Triacylglycerol + H2O = diacylglycerol + a carboxylate.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei169 – 1691NucleophileBy similarity
    Active sitei195 – 1951Charge relay systemPROSITE-ProRule annotation
    Active sitei280 – 2801Charge relay systemPROSITE-ProRule annotation

    GO - Molecular functioni

    1. heparin binding Source: UniProtKB-KW
    2. lipase activity Source: MGI
    3. protein binding Source: MGI
    4. triglyceride lipase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cholesterol homeostasis Source: MGI
    2. cholesterol metabolic process Source: MGI
    3. cholesterol transport Source: MGI
    4. fatty acid biosynthetic process Source: Ensembl
    5. high-density lipoprotein particle remodeling Source: Ensembl
    6. low-density lipoprotein particle remodeling Source: Ensembl
    7. triglyceride catabolic process Source: Ensembl
    8. triglyceride homeostasis Source: Ensembl
    9. very-low-density lipoprotein particle remodeling Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Keywords - Ligandi

    Heparin-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hepatic triacylglycerol lipase (EC:3.1.1.3)
    Short name:
    HL
    Short name:
    Hepatic lipase
    Alternative name(s):
    Lipase member C
    Gene namesi
    Name:Lipc
    Synonyms:Hpl
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 9

    Organism-specific databases

    MGIiMGI:96216. Lipc.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI
    2. high-density lipoprotein particle Source: UniProtKB-KW

    Keywords - Cellular componenti

    HDL, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222By similarityAdd
    BLAST
    Chaini23 – 510488Hepatic triacylglycerol lipasePRO_0000017770Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi79 – 791N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi398 – 3981N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiP27656.
    PRIDEiP27656.

    PTM databases

    PhosphoSiteiP27656.

    Expressioni

    Gene expression databases

    ArrayExpressiP27656.
    BgeeiP27656.
    CleanExiMM_LIPC.
    GenevestigatoriP27656.

    Interactioni

    Protein-protein interaction databases

    BioGridi200409. 27 interactions.
    STRINGi10090.ENSMUSP00000034731.

    Structurei

    3D structure databases

    ProteinModelPortaliP27656.
    SMRiP27656. Positions 37-471.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini353 – 487135PLATPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni183 – 19412Heparin-bindingSequence AnalysisAdd
    BLAST

    Sequence similaritiesi

    Belongs to the AB hydrolase superfamily. Lipase family.Curated
    Contains 1 PLAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG81747.
    GeneTreeiENSGT00750000117234.
    HOGENOMiHOG000038553.
    HOVERGENiHBG002259.
    InParanoidiQ8VC44.
    KOiK01046.
    OMAiCHFLELY.
    OrthoDBiEOG757CX5.
    TreeFamiTF324997.

    Family and domain databases

    Gene3Di2.60.60.20. 1 hit.
    3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000734. Lipase.
    IPR002333. Lipase_hep.
    IPR008976. Lipase_LipOase.
    IPR013818. Lipase_N.
    IPR016272. Lipoprotein_lipase_LIPH.
    IPR001024. PLAT/LH2_dom.
    [Graphical view]
    PANTHERiPTHR11610. PTHR11610. 1 hit.
    PTHR11610:SF2. PTHR11610:SF2. 1 hit.
    PfamiPF00151. Lipase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
    PRINTSiPR00824. HEPLIPASE.
    PR00821. TAGLIPASE.
    SMARTiSM00308. LH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF49723. SSF49723. 1 hit.
    SSF53474. SSF53474. 1 hit.
    PROSITEiPS00120. LIPASE_SER. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27656-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGNPLQISIF LVFCIFIQSS ACGQGVGTEP FGRSLGATEA SKPLKKPETR    50
    FLLFQDENDR LGCRLRPQHP ETLQECGFNS SQPLIMIIHG WSVDGLLENW 100
    IWKIVSALKS RQSQPVNVGL VDWISLAYQH YTIAVQNTRI VGQDVAALLL 150
    WLEESAKFSR SKVHLIGYSL GAHVSGFAGS SMDGKNKIGR ITGLDPAGPM 200
    FEGTSPNERL SPDDANFVDA IHTFTREHMG LSVGIKQPIA HYDFYPNGGS 250
    FQPGCHFLEL YKHIAEHGLN AITQTIKCAH ERSVHLFIDS LQHSDLQSIG 300
    FQCSDMGSFS QGLCLSCKKG RCNTLGYDIR KDRSGKSKRL FLITRAQSPF 350
    KVYHYQFKIQ FINQIEKPVE PTFTMSLLGT KEEIKRIPIT LGEGITSNKT 400
    YSFLITLDKD IGELILLKFK WENSAVWANV WNTVQTIMLW GIEPHHSGLI 450
    LKTIWVKAGE TQQRMTFCPE NLDDLQLHPS QEKVFVNCEV KSKRLTESKE 500
    QMSQETHAKK 510
    Length:510
    Mass (Da):57,389
    Last modified:July 27, 2011 - v2
    Checksum:iE30222652D782A05
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti286 – 2861L → P in CAA41329. (PubMed:1840530)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228765 mRNA. Translation: AAO73443.1.
    X58426 mRNA. Translation: CAA41329.1.
    CH466522 Genomic DNA. Translation: EDL26212.1.
    BC021841 mRNA. Translation: AAH21841.1.
    BC094050 mRNA. Translation: AAH94050.1.
    CCDSiCCDS23324.1.
    PIRiS15893.
    RefSeqiNP_032306.2. NM_008280.2.
    UniGeneiMm.390187.

    Genome annotation databases

    EnsembliENSMUST00000034731; ENSMUSP00000034731; ENSMUSG00000032207.
    GeneIDi15450.
    KEGGimmu:15450.
    UCSCiuc009qos.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AY228765 mRNA. Translation: AAO73443.1 .
    X58426 mRNA. Translation: CAA41329.1 .
    CH466522 Genomic DNA. Translation: EDL26212.1 .
    BC021841 mRNA. Translation: AAH21841.1 .
    BC094050 mRNA. Translation: AAH94050.1 .
    CCDSi CCDS23324.1.
    PIRi S15893.
    RefSeqi NP_032306.2. NM_008280.2.
    UniGenei Mm.390187.

    3D structure databases

    ProteinModelPortali P27656.
    SMRi P27656. Positions 37-471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200409. 27 interactions.
    STRINGi 10090.ENSMUSP00000034731.

    PTM databases

    PhosphoSitei P27656.

    Proteomic databases

    PaxDbi P27656.
    PRIDEi P27656.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000034731 ; ENSMUSP00000034731 ; ENSMUSG00000032207 .
    GeneIDi 15450.
    KEGGi mmu:15450.
    UCSCi uc009qos.1. mouse.

    Organism-specific databases

    CTDi 3990.
    MGIi MGI:96216. Lipc.

    Phylogenomic databases

    eggNOGi NOG81747.
    GeneTreei ENSGT00750000117234.
    HOGENOMi HOG000038553.
    HOVERGENi HBG002259.
    InParanoidi Q8VC44.
    KOi K01046.
    OMAi CHFLELY.
    OrthoDBi EOG757CX5.
    TreeFami TF324997.

    Miscellaneous databases

    NextBioi 288252.
    PROi P27656.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27656.
    Bgeei P27656.
    CleanExi MM_LIPC.
    Genevestigatori P27656.

    Family and domain databases

    Gene3Di 2.60.60.20. 1 hit.
    3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000734. Lipase.
    IPR002333. Lipase_hep.
    IPR008976. Lipase_LipOase.
    IPR013818. Lipase_N.
    IPR016272. Lipoprotein_lipase_LIPH.
    IPR001024. PLAT/LH2_dom.
    [Graphical view ]
    PANTHERi PTHR11610. PTHR11610. 1 hit.
    PTHR11610:SF2. PTHR11610:SF2. 1 hit.
    Pfami PF00151. Lipase. 1 hit.
    PF01477. PLAT. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000865. Lipoprotein_lipase_LIPH. 1 hit.
    PRINTSi PR00824. HEPLIPASE.
    PR00821. TAGLIPASE.
    SMARTi SM00308. LH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49723. SSF49723. 1 hit.
    SSF53474. SSF53474. 1 hit.
    PROSITEi PS00120. LIPASE_SER. 1 hit.
    PS50095. PLAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of mouse hepatic triacylglycerol lipase: gene expression in combined lipase-deficient (cld/cld) mice."
      Oka K., Nakano T., Tkalcevic G.T., Scow R.O., Brown W.V.
      Biochim. Biophys. Acta 1089:13-20(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Characterization of cDNA encoding the mouse hepatic triglyceride lipase and expression by in vitro translation."
      Chang S.F., Netter H.J., Will H.
      FEBS Lett. 289:69-72(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
      Submitted (FEB-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.

    Entry informationi

    Entry nameiLIPC_MOUSE
    AccessioniPrimary (citable) accession number: P27656
    Secondary accession number(s): Q8VC44
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3