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Protein

Temperature shock-inducible protein 1

Gene

TIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Seems to have esterase activity. Prefers ester of fatty acids from 4 to 16 carbon atoms.

Miscellaneous

Present with 35600 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • lipase activity Source: SGD
  • structural constituent of cell wall Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
  • response to stress Source: InterPro

Keywordsi

Molecular functionHydrolase
Biological processStress response

Enzyme and pathway databases

BioCyciYEAST:G3O-29036-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Temperature shock-inducible protein 1 (EC:3.1.1.-)
Gene namesi
Name:TIP1
Ordered Locus Names:YBR067C
ORF Names:YBR0622
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR067C
SGDiS000000271 TIP1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 194 PublicationsAdd BLAST19
ChainiPRO_000003323720 – 186Temperature shock-inducible protein 1Add BLAST167
PropeptideiPRO_0000033238187 – 210Removed in mature formAdd BLAST24

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi186GPI-anchor amidated asparagine1 Publication1

Post-translational modificationi

Extensively O-glycosylated.2 Publications
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Proteomic databases

PaxDbiP27654
PRIDEiP27654

Expressioni

Inductioni

Induced during anaerobic growth and by cold shock and heat shock.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi32770, 34 interactors
DIPiDIP-4908N
IntActiP27654, 8 interactors
MINTiP27654
STRINGi4932.YBR067C

Structurei

3D structure databases

ProteinModelPortaliP27654
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi101 – 120Ala/Ser-richAdd BLAST20

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00700000104882
InParanoidiP27654
OMAiATELPWY
OrthoDBiEOG092C5NOA

Family and domain databases

InterProiView protein in InterPro
IPR000992 SRP1_TIP1
PfamiView protein in Pfam
PF00660 SRP1_TIP1, 1 hit
PROSITEiView protein in PROSITE
PS00724 SRP1_TIP1, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSKIAFVL SAIASLAVAD TSAAETAELQ AIIGDINSHL SDYLGLETGN
60 70 80 90 100
SGFQIPSDVL SVYQQVMTYT DDAYTTLFSE LDFDAITKTI VKLPWYTTRL
110 120 130 140 150
SSEIAAALAS VSPASSEAAS SSEAASSSKA ASSSEATSSA APSSSAAPSS
160 170 180 190 200
SAAPSSSAES SSKAVSSSVA PTTSSVSTST VETASNAGQR VNAGAASFGA
210
VVAGAAALLL
Length:210
Mass (Da):20,728
Last modified:August 1, 1992 - v1
Checksum:iD607F34A9C1D108F
GO

Mass spectrometryi

Molecular mass is 16944 Da from positions 20 - 188. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M71216 Genomic DNA Translation: AAA35157.1
Z35936 Genomic DNA Translation: CAA85011.1
BK006936 Genomic DNA Translation: DAA07186.1
PIRiA40979
RefSeqiNP_009623.1, NM_001178415.1

Genome annotation databases

EnsemblFungiiYBR067C; YBR067C; YBR067C
GeneIDi852359
KEGGisce:YBR067C

Similar proteinsi

Entry informationi

Entry nameiTIP1_YEAST
AccessioniPrimary (citable) accession number: P27654
Secondary accession number(s): D6VQ66
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: March 28, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health