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Protein

Temperature shock-inducible protein 1

Gene

TIP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Seems to have esterase activity. Prefers ester of fatty acids from 4 to 16 carbon atoms.

GO - Molecular functioni

  • lipase activity Source: SGD
  • structural constituent of cell wall Source: SGD

GO - Biological processi

  • fungal-type cell wall organization Source: SGD
  • response to stress Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciYEAST:G3O-29036-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Temperature shock-inducible protein 1 (EC:3.1.1.-)
Gene namesi
Name:TIP1
Ordered Locus Names:YBR067C
ORF Names:YBR0622
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR067C.
SGDiS000000271. TIP1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19194 PublicationsAdd
BLAST
Chaini20 – 186167Temperature shock-inducible protein 1PRO_0000033237Add
BLAST
Propeptidei187 – 21024Removed in mature formPRO_0000033238Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi186 – 1861GPI-anchor amidated asparagine1 Publication

Post-translational modificationi

Extensively O-glycosylated.2 Publications
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Inductioni

Induced during anaerobic growth and by cold shock and heat shock.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi32770. 16 interactions.
DIPiDIP-4908N.
IntActiP27654. 3 interactions.
MINTiMINT-476413.

Structurei

3D structure databases

ProteinModelPortaliP27654.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi101 – 12020Ala/Ser-richAdd
BLAST

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

GeneTreeiENSGT00700000104882.
InParanoidiP27654.
OMAiTIVKLPW.
OrthoDBiEOG092C5NOA.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVSKIAFVL SAIASLAVAD TSAAETAELQ AIIGDINSHL SDYLGLETGN
60 70 80 90 100
SGFQIPSDVL SVYQQVMTYT DDAYTTLFSE LDFDAITKTI VKLPWYTTRL
110 120 130 140 150
SSEIAAALAS VSPASSEAAS SSEAASSSKA ASSSEATSSA APSSSAAPSS
160 170 180 190 200
SAAPSSSAES SSKAVSSSVA PTTSSVSTST VETASNAGQR VNAGAASFGA
210
VVAGAAALLL
Length:210
Mass (Da):20,728
Last modified:August 1, 1992 - v1
Checksum:iD607F34A9C1D108F
GO

Mass spectrometryi

Molecular mass is 16944 Da from positions 20 - 188. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M71216 Genomic DNA. Translation: AAA35157.1.
Z35936 Genomic DNA. Translation: CAA85011.1.
BK006936 Genomic DNA. Translation: DAA07186.1.
PIRiA40979.
RefSeqiNP_009623.1. NM_001178415.1.

Genome annotation databases

EnsemblFungiiYBR067C; YBR067C; YBR067C.
GeneIDi852359.
KEGGisce:YBR067C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M71216 Genomic DNA. Translation: AAA35157.1.
Z35936 Genomic DNA. Translation: CAA85011.1.
BK006936 Genomic DNA. Translation: DAA07186.1.
PIRiA40979.
RefSeqiNP_009623.1. NM_001178415.1.

3D structure databases

ProteinModelPortaliP27654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi32770. 16 interactions.
DIPiDIP-4908N.
IntActiP27654. 3 interactions.
MINTiMINT-476413.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR067C; YBR067C; YBR067C.
GeneIDi852359.
KEGGisce:YBR067C.

Organism-specific databases

EuPathDBiFungiDB:YBR067C.
SGDiS000000271. TIP1.

Phylogenomic databases

GeneTreeiENSGT00700000104882.
InParanoidiP27654.
OMAiTIVKLPW.
OrthoDBiEOG092C5NOA.

Enzyme and pathway databases

BioCyciYEAST:G3O-29036-MONOMER.

Miscellaneous databases

PROiP27654.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTIP1_YEAST
AccessioniPrimary (citable) accession number: P27654
Secondary accession number(s): D6VQ66
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 35600 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.