Reviewed, UniProtKB/Swiss-Prot P27652 (LUCI_RENRE)

Names and origin

Protein names	Recommended name:     Renilla-luciferin 2-monooxygenase     EC=1.13.12.5 Alternative name(s):     Renilla-type luciferase
Organism	Renilla reniformis (Sea pansy)
Taxonomic identifier	6136 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Cnidaria › Anthozoa › Octocorallia › Pennatulacea › Sessiliflorae › Renillidae › Renilla

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light.
Subunit structure	Monomer.
Miscellaneous	This luciferase produces light with a wavelength of 480 nm. In presence of a green fluorescence protein (GFP) it produces a green fluorescence at 509 nm.

Ontologies

Keywords
Biological process	Luminescence
Molecular function	Decarboxylase Lyase Monooxygenase Oxidoreductase Photoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Renilla-luciferin 2-monooxygenase activity Inferred from electronic annotation. Source: EC carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 311

311

Renilla-luciferin 2-monooxygenase

PRO_0000084523

Experimental info

Sequence conflict

219

1

W → L AA sequence Ref.1

Secondary structure

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311

Helix Strand Turn

Details...

Helix

10 – 13

4

Helix

17 – 23

7

Beta strand

25 – 29

5

Beta strand

32 – 38

7

Beta strand

45 – 50

6

Helix

57 – 60

4

Turn

61 – 63

3

Helix

64 – 66

3

Turn

67 – 70

4

Beta strand

71 – 76

6

Helix

94 – 105

12

Beta strand

112 – 119

8

Helix

121 – 132

12

Beta strand

136 – 144

9

Helix

160 – 167

8

Helix

170 – 175

6

Turn

176 – 178

3

Helix

180 – 183

4

Helix

185 – 188

4

Helix

196 – 203

8

Helix

204 – 206

3

Beta strand

208 – 210

3

Helix

211 – 213

3

Helix

214 – 221

8

Turn

226 – 228

3

Helix

231 – 245

15

Beta strand

252 – 259

8

Helix

263 – 270

8

Beta strand

273 – 286

14

Helix

287 – 289

3

Helix

292 – 307

16

Sequences

	Sequence		Length	Mass (Da)
	P27652-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 0A3FD025B4EC33FD	FASTA	311	36,022
	10 20 30 40 50 60 MTSKVYDPEQ RKRMITGPQW WARCKQMNVL DSFINYYDSE KHAENAVIFL HGNAASSYLW 70 80 90 100 110 120 RHVVPHIEPV ARCIIPDLIG MGKSGKSGNG SYRLLDHYKY LTAWFELLNL PKKIIFVGHD 130 140 150 160 170 180 WGACLAFHYS YEHQDKIKAI VHAESVVDVI ESWDEWPDIE EDIALIKSEE GEKMVLENNF 190 200 210 220 230 240 FVETMLPSKI MRKLEPEEFA AYLEPFKEKG EVRRPTLSWP REIPLVKGGK PDVVQIVRNY 250 260 270 280 290 300 NAYLRASDDL PKMFIESDPG FFSNAIVEGA KKFPNTEFVK VKGLHFSQED APDEMGKYIK 310 SFVERVLKNE Q

References

[1]	"Isolation and expression of a cDNA encoding Renilla reniformis luciferase." Lorenz W.W., McCann R.O., Longiaru M., Cormier M.J. Proc. Natl. Acad. Sci. U.S.A. 88:4438-4442(1991) [PubMed: 1674607] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

M63501 mRNA. Translation: AAA29804.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PSD	X-ray	1.40	A	3-311	[»]
2PSE	X-ray	2.50	A	3-311	[»]
2PSF	X-ray	1.40	A/B	3-311	[»]
2PSH	X-ray	1.79	A/B	3-311	[»]
2PSJ	X-ray	1.80	A/B	3-311	[»]

ModBase

Search...

Enzyme and pathway databases

BRENDA

1.13.12.5. 260671.

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PRINTS

PR00412. EPOXHYDRLASE.

ProtoNet

Search...

Entry information

Entry name

LUCI_RENRE

Accession

Primary (citable) accession number: P27652

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 26, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)