P27652 (LUCI_RENRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Renilla-luciferin 2-monooxygenase EC=1.13.12.5 Alternative name(s): Renilla-type luciferase
Organism	Renilla reniformis (Sea pansy)
Taxonomic identifier	6136 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Cnidaria › Anthozoa › Octocorallia › Pennatulacea › Sessiliflorae › Renillidae › Renilla

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Renilla luciferin + O2 = oxidized Renilla luciferin + CO2 + light.
Subunit structure	Monomer.
Miscellaneous	This luciferase produces light with a wavelength of 480 nm. In presence of a green fluorescence protein (GFP) it produces a green fluorescence at 509 nm.

Ontologies

Keywords
Biological process	Luminescence
Molecular function	Decarboxylase Lyase Monooxygenase Oxidoreductase Photoprotein
Technical term	3D-structure Direct protein sequencing
Gene Ontology (GO)
Biological_process	bioluminescence Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	Renilla-luciferin 2-monooxygenase activity Inferred from electronic annotation. Source: EC carboxy-lyase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 311

311

Renilla-luciferin 2-monooxygenase

PRO_0000084523

Experimental info

Sequence conflict

219

1

W → L AA sequence Ref.1

Secondary structure

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311

Helix Strand Turn

Details...

Helix

10 – 13

4

Helix

17 – 23

7

Beta strand

25 – 29

5

Beta strand

32 – 38

7

Beta strand

45 – 50

6

Helix

57 – 60

4

Turn

61 – 63

3

Helix

64 – 66

3

Turn

67 – 70

4

Beta strand

71 – 76

6

Helix

94 – 105

12

Turn

106 – 109

4

Beta strand

112 – 119

8

Helix

121 – 132

12

Beta strand

136 – 144

9

Helix

160 – 167

8

Helix

170 – 175

6

Turn

176 – 178

3

Helix

180 – 183

4

Helix

185 – 188

4

Beta strand

191 – 193

3

Helix

196 – 203

8

Helix

204 – 206

3

Beta strand

208 – 210

3

Helix

211 – 213

3

Helix

214 – 221

8

Turn

226 – 228

3

Helix

231 – 245

15

Beta strand

252 – 259

8

Helix

263 – 270

8

Beta strand

273 – 286

14

Helix

287 – 289

3

Helix

292 – 307

16

Sequences

	Sequence		Length	Mass (Da)
	P27652 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 0A3FD025B4EC33FD	FASTA	311	36,022
	10 20 30 40 50 60 MTSKVYDPEQ RKRMITGPQW WARCKQMNVL DSFINYYDSE KHAENAVIFL HGNAASSYLW 70 80 90 100 110 120 RHVVPHIEPV ARCIIPDLIG MGKSGKSGNG SYRLLDHYKY LTAWFELLNL PKKIIFVGHD 130 140 150 160 170 180 WGACLAFHYS YEHQDKIKAI VHAESVVDVI ESWDEWPDIE EDIALIKSEE GEKMVLENNF 190 200 210 220 230 240 FVETMLPSKI MRKLEPEEFA AYLEPFKEKG EVRRPTLSWP REIPLVKGGK PDVVQIVRNY 250 260 270 280 290 300 NAYLRASDDL PKMFIESDPG FFSNAIVEGA KKFPNTEFVK VKGLHFSQED APDEMGKYIK 310 SFVERVLKNE Q

References

[1]	"Isolation and expression of a cDNA encoding Renilla reniformis luciferase." Lorenz W.W., McCann R.O., Longiaru M., Cormier M.J. Proc. Natl. Acad. Sci. U.S.A. 88:4438-4442(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M63501 mRNA. Translation: AAA29804.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2PSD	X-ray	1.40	A	3-311	[»]
2PSE	X-ray	2.50	A	3-311	[»]
2PSF	X-ray	1.40	A/B	3-311	[»]
2PSH	X-ray	1.79	A/B	3-311	[»]
2PSJ	X-ray	1.80	A/B	3-311	[»]

ProteinModelPortal

P27652.

SMR

P27652. Positions 3-309.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-16919.

Family and domain databases

InterPro

IPR000073. AB_hydrolase_1.
IPR000639. Epox_hydrolase-like.
[Graphical view]

Pfam

PF00561. Abhydrolase_1. 1 hit.
[Graphical view]

PRINTS

PR00412. EPOXHYDRLASE.

ProtoNet

Search...

Other

EvolutionaryTrace

P27652.

Entry information

Entry name

LUCI_RENRE

Accession

Primary (citable) accession number: P27652

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)