Reviewed, UniProtKB/Swiss-Prot P27644 (PGLR_AGRTU)

Names and origin

Protein names

Recommended name:
    Polygalacturonase
    EC=3.2.1.15
Alternative name(s):
    Pectinase
    PGL

Gene names

Name:

pgl

Organism

Agrobacterium tumefaciens

Taxonomic identifier

358 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Rhizobiaceae › Rhizobium/Agrobacterium group › Agrobacterium

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Seems to regulates the surface properties of the bacterium in the presence of plant cells or plant cell extracts.
Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Subcellular location	Secreted.
Induction	By certain acidic polysaccharides found in carrot root extract.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 4 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	polygalacturonase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Polygalacturonase		PRO_0000215228
Sites
	Active site	94	1	Proton donor By similarity
	Active site	130	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27644-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 37B03C2EE29F23AB	FASTA	312	32,943
	10 20 30 40 50 60 MALATRATGG AGRRKPVRAR CARGLHLVSC HKTQLLGFTI RNAASWTIHP QGCEDLTAAA 70 80 90 100 110 120 STIIAPHDSP NTDGFNPESC RNVMISGVRF SVGDDCIAVK AGKRGPDGED DHLAETRGIT 130 140 150 160 170 180 VRHCLMQPGH GGLVIGSEMS GGVHDVTVED CDMIGTDRGL RLKTGARSGG GMVGNITMRR 190 200 210 220 230 240 VLLDGVQTAL SANAHYHCDA DGHDDWVQSR NPAPVNDGTP FVDGITVEDV EIRNLAHAAG 250 260 270 280 290 300 VFLGLPDVPS ATSLSATSPI VSHDPSAVAT PPIMADRVRP MRMRLVFEQA DVVCDDPALL 310 NDAPVSISSY FD

References

[1]	"Genetic and molecular analyses of picA, a plant-inducible locus on the Agrobacterium tumefaciens chromosome." Rong L., Karcher S.J., Gelvin S.B. J. Bacteriol. 173:5110-5120(1991) [PubMed: 1860822] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: A136.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M62814 Genomic DNA. Translation: AAA22102.1.
PIR	A40364.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Enzyme and pathway databases
BRENDA	3.2.1.15. 2199.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 4 hits. [Graphical view]
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PGLR_AGRTU

Accession

Primary (citable) accession number: P27644

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)