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Protein

Eukaryotic initiation factor 4A

Gene

inf-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi73 – 80ATP8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase, Initiation factor, RNA-binding
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-1169408. ISG15 antiviral mechanism.
R-CEL-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-CEL-72662. Activation of the mRNA upon binding of the cap-binding complex and eIFs, and subsequent binding to 43S.
R-CEL-72702. Ribosomal scanning and start codon recognition.

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic initiation factor 4A (EC:3.6.4.13)
Short name:
eIF-4A
Alternative name(s):
ATP-dependent RNA helicase eIF4A
Initiation factor 1
Gene namesi
Name:inf-1
ORF Names:F57B9.6
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome III

Organism-specific databases

WormBaseiF57B9.6a; CE01341; WBGene00002083; inf-1.
F57B9.6b; CE38524; WBGene00002083; inf-1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000549451 – 402Eukaryotic initiation factor 4AAdd BLAST402

Proteomic databases

EPDiP27639.
PaxDbiP27639.
PeptideAtlasiP27639.
PRIDEiP27639.

Expressioni

Gene expression databases

BgeeiWBGene00002083.

Interactioni

Subunit structurei

eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G (By similarity).By similarity

Protein-protein interaction databases

BioGridi41180. 1 interactor.
DIPiDIP-27102N.
IntActiP27639. 1 interactor.
MINTiMINT-1112147.
STRINGi6239.F57B9.6a.2.

Structurei

3D structure databases

ProteinModelPortaliP27639.
SMRiP27639.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini60 – 230Helicase ATP-bindingAdd BLAST171
Domaini241 – 402Helicase C-terminalAdd BLAST162

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi29 – 57Q motifAdd BLAST29
Motifi178 – 181DEAD box4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0327. Eukaryota.
COG0513. LUCA.
GeneTreeiENSGT00530000062880.
HOGENOMiHOG000268797.
InParanoidiP27639.
KOiK03257.
OMAiFGCQALV.
OrthoDBiEOG091G07OI.
PhylomeDBiP27639.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
InterProiView protein in InterPro
IPR011545. DEAD/DEAH_box_helicase_dom.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000629. RNA-helicase_DEAD-box_CS.
IPR014014. RNA_helicase_DEAD_Q_motif.
PfamiView protein in Pfam
PF00270. DEAD. 1 hit.
PF00271. Helicase_C. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiView protein in PROSITE
PS00039. DEAD_ATP_HELICASE. 1 hit.
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS51195. Q_MOTIF. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a (identifier: P27639-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDVKNDVNV SSVVDADGLI EGNYDQVVES FDDMELKEEL LRGIYGFGFE
60 70 80 90 100
KPSAIQKRAI VPCTTGKDVI AQAQSGTGKT ATFSVSILQR IDHEDPHVQA
110 120 130 140 150
LVMAPTRELA QQIQKVMSAL GEYLNVNILP CIGGTSVRDD QRKLEAGIHV
160 170 180 190 200
VVGTPGRVGD MINRNALDTS RIKMFVLDEA DEMLSRGFKD QIYEVFRSMP
210 220 230 240 250
QDVQVVLLSA TMPSEVLDVT NRFMRNPIRI LVKKDELTLE GIRQFYINVQ
260 270 280 290 300
KDEWKFDCLC DLYNVVNVTQ AVIFCNTRRK VDTLTEKMTE NQFTVSCLHG
310 320 330 340 350
DMDQAERDTI MREFRSGSSR VLITTDILAR GIDVQQVSLV INYDLPSNRE
360 370 380 390 400
NYIHRIGRSG RFGRKGVAIN FVTENDARQL KEIESYYTTQ IEEMPESIAD

LI
Length:402
Mass (Da):45,408
Last modified:August 1, 1992 - v1
Checksum:iAB1CE281F9FC8881
GO
Isoform b (identifier: P27639-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-287: Missing.

Note: No experimental confirmation available.
Show »
Length:115
Mass (Da):13,187
Checksum:iE91B226E8F907340
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0206401 – 287Missing in isoform b. CuratedAdd BLAST287

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z12116 mRNA. Translation: CAA78102.1.
FO081266 Genomic DNA. Translation: CCD70313.1.
FO081266 Genomic DNA. Translation: CCD70314.1.
PIRiS26281.
RefSeqiNP_001022623.1. NM_001027452.2. [P27639-1]
NP_001022624.1. NM_001027453.2. [P27639-2]
UniGeneiCel.6803.

Genome annotation databases

EnsemblMetazoaiF57B9.6a.1; F57B9.6a.1; WBGene00002083. [P27639-1]
F57B9.6a.2; F57B9.6a.2; WBGene00002083. [P27639-1]
GeneIDi175966.
KEGGicel:CELE_F57B9.6.
UCSCiF57B9.6a.1. c. elegans. [P27639-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiIF4A_CAEEL
AccessioniPrimary (citable) accession number: P27639
Secondary accession number(s): Q4TTC4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 25, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Translation initiation factors
    List of translation initiation factor entries
  3. SIMILARITY comments
    Index of protein domains and families