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P27638

- SPK1_SCHPO

UniProt

P27638 - SPK1_SCHPO

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Protein

Mitogen-activated protein kinase spk1

Gene

spk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Involved in mating signal transduction pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei68 – 681ATPPROSITE-ProRule annotation
Active sitei163 – 1631Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 539ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: PomBase
  3. protein kinase activity Source: PomBase

GO - Biological processi

  1. cell cycle arrest in response to pheromone Source: PomBase
  2. MAPK cascade involved in conjugation with cellular fusion Source: PomBase
  3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
  4. signal transduction involved in conjugation with cellular fusion Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 5615.
ReactomeiREACT_202608. ERK2 activation.
REACT_202848. Signaling by FGFR.
REACT_205526. ERK1 activation.
REACT_213398. Regulation of HSF1-mediated heat shock response.
REACT_224814. Signal transduction by L1.
REACT_231661. ERKs are inactivated.
REACT_260281. ERK/MAPK targets.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase spk1 (EC:2.7.11.24)
Short name:
MAP kinase spk1
Short name:
MAPK
Gene namesi
Name:spk1
ORF Names:SPAC31G5.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
ProteomesiUP000002485: Chromosome I

Organism-specific databases

PomBaseiSPAC31G5.09c.

Subcellular locationi

Nucleus
Note: Mainly nuclear.

GO - Cellular componenti

  1. cytosol Source: PomBase
  2. mitotic spindle pole body Source: PomBase
  3. nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Mitogen-activated protein kinase spk1PRO_0000186340Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei199 – 1991PhosphothreonineBy similarity
Modified residuei201 – 2011PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27638.

Interactioni

Protein-protein interaction databases

BioGridi278934. 7 interactions.
IntActiP27638. 3 interactions.
MINTiMINT-4688010.
STRINGi4896.SPAC31G5.09c-1.

Structurei

3D structure databases

ProteinModelPortaliP27638.
SMRiP27638. Positions 31-369.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 327289Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi199 – 2013TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
InParanoidiP27638.
KOiK04371.
OMAiHENIISV.
OrthoDBiEOG7K3TWD.
PhylomeDBiP27638.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01773. P38MAPKINASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27638-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY
60 70 80 90 100
GVVCAALHKP SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI
110 120 130 140 150
LDILPPPSYQ ELEDVYIVQE LMETDLYRVI RSQPLSDDHC QYFTYQILRA
160 170 180 190 200
LKAMHSAGVV HRDLKPSNLL LNANCDLKVA DFGLARSTTA QGGNPGFMTE
210 220 230 240 250
YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP LFPGKDYHSQ
260 270 280 290 300
ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA
310 320 330 340 350
IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY
360 370
CNKEDLEIPV LKALIFREVN FR
Length:372
Mass (Da):42,003
Last modified:August 1, 1992 - v1
Checksum:i85D980514C9386C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31735 Genomic DNA. Translation: BAA06536.1.
X57334 Genomic DNA. Translation: CAA40610.1.
AB084886 mRNA. Translation: BAC54906.1.
AB084887 mRNA. Translation: BAC54907.1.
CU329670 Genomic DNA. Translation: CAB11693.1.
AB004551 Genomic DNA. Translation: BAA20417.1.
PIRiS15663.
RefSeqiNP_594009.1. NM_001019435.2.

Genome annotation databases

EnsemblFungiiSPAC31G5.09c.1; SPAC31G5.09c.1:pep; SPAC31G5.09c.
GeneIDi2542474.
KEGGispo:SPAC31G5.09c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31735 Genomic DNA. Translation: BAA06536.1 .
X57334 Genomic DNA. Translation: CAA40610.1 .
AB084886 mRNA. Translation: BAC54906.1 .
AB084887 mRNA. Translation: BAC54907.1 .
CU329670 Genomic DNA. Translation: CAB11693.1 .
AB004551 Genomic DNA. Translation: BAA20417.1 .
PIRi S15663.
RefSeqi NP_594009.1. NM_001019435.2.

3D structure databases

ProteinModelPortali P27638.
SMRi P27638. Positions 31-369.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 278934. 7 interactions.
IntActi P27638. 3 interactions.
MINTi MINT-4688010.
STRINGi 4896.SPAC31G5.09c-1.

Proteomic databases

MaxQBi P27638.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii SPAC31G5.09c.1 ; SPAC31G5.09c.1:pep ; SPAC31G5.09c .
GeneIDi 2542474.
KEGGi spo:SPAC31G5.09c.

Organism-specific databases

PomBasei SPAC31G5.09c.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
InParanoidi P27638.
KOi K04371.
OMAi HENIISV.
OrthoDBi EOG7K3TWD.
PhylomeDBi P27638.

Enzyme and pathway databases

BRENDAi 2.7.11.24. 5615.
Reactomei REACT_202608. ERK2 activation.
REACT_202848. Signaling by FGFR.
REACT_205526. ERK1 activation.
REACT_213398. Regulation of HSF1-mediated heat shock response.
REACT_224814. Signal transduction by L1.
REACT_231661. ERKs are inactivated.
REACT_260281. ERK/MAPK targets.

Miscellaneous databases

NextBioi 20803529.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01773. P38MAPKINASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases."
    Toda T., Shimanuki M., Yanagida M.
    Genes Dev. 5:60-73(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of abundant polyA plus non-coding RNAs that are expressed in Schizosaccharomyces pombe."
    Watanabe T., Saito T.T., Nabeshima K., Nojima H.
    Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: CD16-1.
  3. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  4. "S.pombe uroporphrinogen III synthase gene."
    Kawamukai M.
    Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-372.
  5. "Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase."
    Gotoh Y., Nishida E., Shimanuki M., Toda T., Imai Y., Yamamoto M.
    Mol. Cell. Biol. 13:6427-6434(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiSPK1_SCHPO
AccessioniPrimary (citable) accession number: P27638
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to confer resistance to staurosporine.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3