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P27638 (SPK1_SCHPO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 129. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase spk1

Short name=MAP kinase spk1
Short name=MAPK
EC=2.7.11.24
Gene names
Name:spk1
ORF Names:SPAC31G5.09c
OrganismSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) [Reference proteome]
Taxonomic identifier284812 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces

Protein attributes

Sequence length372 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Involved in mating signal transduction pathway. Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Magnesium By similarity.

Enzyme regulation

Activated by tyrosine and threonine phosphorylation.

Subcellular location

Nucleus. Note: Mainly nuclear.

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Caution

Was originally (Ref.1) thought to confer resistance to staurosporine.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 372372Mitogen-activated protein kinase spk1
PRO_0000186340

Regions

Domain39 – 327289Protein kinase
Nucleotide binding45 – 539ATP By similarity
Motif199 – 2013TXY

Sites

Active site1631Proton acceptor By similarity
Binding site681ATP By similarity

Amino acid modifications

Modified residue1991Phosphothreonine By similarity
Modified residue2011Phosphotyrosine By similarity

Sequences

Sequence LengthMass (Da)Tools
P27638 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 85D980514C9386C7

FASTA37242,003
        10         20         30         40         50         60 
MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY GVVCAALHKP 

        70         80         90        100        110        120 
SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI LDILPPPSYQ ELEDVYIVQE 

       130        140        150        160        170        180 
LMETDLYRVI RSQPLSDDHC QYFTYQILRA LKAMHSAGVV HRDLKPSNLL LNANCDLKVA 

       190        200        210        220        230        240 
DFGLARSTTA QGGNPGFMTE YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP 

       250        260        270        280        290        300 
LFPGKDYHSQ ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA 

       310        320        330        340        350        360 
IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY CNKEDLEIPV 

       370 
LKALIFREVN FR 

« Hide

References

« Hide 'large scale' references
[1]"Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases."
Toda T., Shimanuki M., Yanagida M.
Genes Dev. 5:60-73(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of abundant polyA plus non-coding RNAs that are expressed in Schizosaccharomyces pombe."
Watanabe T., Saito T.T., Nabeshima K., Nojima H.
Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: CD16-1.
[3]"The genome sequence of Schizosaccharomyces pombe."
Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. expand/collapse author list , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 972 / ATCC 24843.
[4]"S.pombe uroporphrinogen III synthase gene."
Kawamukai M.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-372.
[5]"Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase."
Gotoh Y., Nishida E., Shimanuki M., Toda T., Imai Y., Yamamoto M.
Mol. Cell. Biol. 13:6427-6434(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D31735 Genomic DNA. Translation: BAA06536.1.
X57334 Genomic DNA. Translation: CAA40610.1.
AB084886 mRNA. Translation: BAC54906.1.
AB084887 mRNA. Translation: BAC54907.1.
CU329670 Genomic DNA. Translation: CAB11693.1.
AB004551 Genomic DNA. Translation: BAA20417.1.
PIRS15663.
RefSeqNP_594009.1. NM_001019435.2.

3D structure databases

ProteinModelPortalP27638.
SMRP27638. Positions 31-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid278934. 5 interactions.
IntActP27638. 3 interactions.
MINTMINT-4688010.
STRING4896.SPAC31G5.09c-1.

Proteomic databases

MaxQBP27638.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiSPAC31G5.09c.1; SPAC31G5.09c.1:pep; SPAC31G5.09c.
GeneID2542474.
KEGGspo:SPAC31G5.09c.

Organism-specific databases

PomBaseSPAC31G5.09c.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
KOK04371.
OMAHENIISV.
OrthoDBEOG7K3TWD.
PhylomeDBP27638.

Enzyme and pathway databases

BRENDA2.7.11.24. 5615.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSPR01773. P38MAPKINASE.
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20803529.

Entry information

Entry nameSPK1_SCHPO
AccessionPrimary (citable) accession number: P27638
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Schizosaccharomyces pombe

Schizosaccharomyces pombe: entries and gene names