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Protein

Mitogen-activated protein kinase spk1

Gene

spk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Involved in mating signal transduction pathway.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68ATPPROSITE-ProRule annotation1
Active sitei163Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 53ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • MAPK cascade involved in ascospore formation Source: PomBase
  • MAPK cascade involved in conjugation with cellular fusion Source: PomBase
  • mitotic cell cycle G1 arrest in response to pheromone Source: PomBase
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24. 5613.
ReactomeiR-SPO-110056. MAPK3 (ERK1) activation.
R-SPO-112409. RAF-independent MAPK1/3 activation.
R-SPO-112411. MAPK1 (ERK2) activation.
R-SPO-198753. ERK/MAPK targets.
R-SPO-202670. ERKs are inactivated.
R-SPO-3371453. Regulation of HSF1-mediated heat shock response.
R-SPO-445144. Signal transduction by L1.
R-SPO-5673001. RAF/MAP kinase cascade.
R-SPO-5674135. MAP2K and MAPK activation.
R-SPO-5674499. Negative feedback regulation of MAPK pathway.
R-SPO-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase spk1 (EC:2.7.11.24)
Short name:
MAP kinase spk1
Short name:
MAPK
Gene namesi
Name:spk1
ORF Names:SPAC31G5.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.09c.
PomBaseiSPAC31G5.09c. spk1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • cytosol Source: PomBase
  • mitotic spindle pole body Source: PomBase
  • nucleus Source: PomBase

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863401 – 372Mitogen-activated protein kinase spk1Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphothreonineBy similarity1
Modified residuei201PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27638.
PRIDEiP27638.

Interactioni

Protein-protein interaction databases

BioGridi278934. 12 interactors.
IntActiP27638. 3 interactors.
MINTiMINT-4688010.
STRINGi4896.SPAC31G5.09c.1.

Structurei

3D structure databases

ProteinModelPortaliP27638.
SMRiP27638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 327Protein kinasePROSITE-ProRule annotationAdd BLAST289

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi199 – 201TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233024.
InParanoidiP27638.
KOiK04371.
OMAiEWKQHIY.
OrthoDBiEOG092C2FL8.
PhylomeDBiP27638.

Family and domain databases

InterProiView protein in InterPro
IPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR008352. MAPK_p38.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF00069. Pkinase. 1 hit.
PRINTSiPR01773. P38MAPKINASE.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

P27638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY
60 70 80 90 100
GVVCAALHKP SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI
110 120 130 140 150
LDILPPPSYQ ELEDVYIVQE LMETDLYRVI RSQPLSDDHC QYFTYQILRA
160 170 180 190 200
LKAMHSAGVV HRDLKPSNLL LNANCDLKVA DFGLARSTTA QGGNPGFMTE
210 220 230 240 250
YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP LFPGKDYHSQ
260 270 280 290 300
ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA
310 320 330 340 350
IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY
360 370
CNKEDLEIPV LKALIFREVN FR
Length:372
Mass (Da):42,003
Last modified:August 1, 1992 - v1
Checksum:i85D980514C9386C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31735 Genomic DNA. Translation: BAA06536.1.
X57334 Genomic DNA. Translation: CAA40610.1.
AB084886 mRNA. Translation: BAC54906.1.
AB084887 mRNA. Translation: BAC54907.1.
CU329670 Genomic DNA. Translation: CAB11693.1.
AB004551 Genomic DNA. Translation: BAA20417.1.
PIRiS15663.
RefSeqiNP_594009.1. NM_001019435.2.

Genome annotation databases

EnsemblFungiiSPAC31G5.09c.1; SPAC31G5.09c.1:pep; SPAC31G5.09c.
GeneIDi2542474.
KEGGispo:SPAC31G5.09c.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSPK1_SCHPO
AccessioniPrimary (citable) accession number: P27638
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 5, 2017
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to confer resistance to staurosporine.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families