Reviewed, UniProtKB/Swiss-Prot P27638 (SPK1_SCHPO)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase spk1
      Short name=MAP kinase spk1
      Short name=MAPK
    EC=2.7.11.24

Gene names

Name:	spk1
ORF Names:	SPAC31G5.09c

Organism

Schizosaccharomyces pombe (Fission yeast) [Complete proteome]

Taxonomic identifier

4896 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Taphrinomycotina › Schizosaccharomycetes › Schizosaccharomycetales › Schizosaccharomycetaceae › Schizosaccharomyces

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in mating signal transduction pathway. Ref.5
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Cofactor	Magnesium By similarity.
Enzyme regulation	Activated by tyrosine and threonine phosphorylation.
Subcellular location	Nucleus. Note: Mainly nuclear.
Domain	The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational modification	Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
Caution	Was originally (Ref.1) thought to confer resistance to staurosporine.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	MAPKKK cascade Inferred by curator. Source: GeneDB_SPombe cell cycle arrest in response to pheromone Traceable author statement. Source: GeneDB_SPombe protein amino acid phosphorylation Inferred by curator. Source: GeneDB_SPombe signal transduction involved in conjugation with cellular fusion Inferred from genetic interaction. Source: GeneDB_SPombe
Cellular component	cytosol Inferred from direct assay. Source: GeneDB_SPombe nucleus Ref.1 Inferred from direct assay. Source: GeneDB_SPombe
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase activity Inferred from genetic interaction. Source: GeneDB_SPombe protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
byr1	P10506	1	EBI-2104356,EBI-2042633

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Mitogen-activated protein kinase spk1		PRO_0000186340
Regions
	Domain	39 – 327	289	Protein kinase
	Nucleotide binding	45 – 53	9	ATP By similarity
	Motif	199 – 201	3	TXY
Sites
	Active site	163	1	Proton acceptor By similarity
	Binding site	68	1	ATP By similarity
Amino acid modifications
	Modified residue	199	1	Phosphothreonine By similarity
	Modified residue	201	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27638-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 85D980514C9386C7	FASTA	372	42,003
	10 20 30 40 50 60 MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY GVVCAALHKP 70 80 90 100 110 120 SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI LDILPPPSYQ ELEDVYIVQE 130 140 150 160 170 180 LMETDLYRVI RSQPLSDDHC QYFTYQILRA LKAMHSAGVV HRDLKPSNLL LNANCDLKVA 190 200 210 220 230 240 DFGLARSTTA QGGNPGFMTE YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP 250 260 270 280 290 300 LFPGKDYHSQ ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA 310 320 330 340 350 360 IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY CNKEDLEIPV 370 LKALIFREVN FR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases." Toda T., Shimanuki M., Yanagida M. Genes Dev. 5:60-73(1991) [PubMed: 1899230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of abundant polyA plus non-coding RNAs that are expressed in Schizosaccharomyces pombe." Watanabe T., Saito T.T., Nabeshima K., Nojima H. Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: CD16-1.
[3]	"The genome sequence of Schizosaccharomyces pombe." Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M. , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P. Nature 415:871-880(2002) [PubMed: 11859360] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 38366 / 972.
[4]	"S.pombe uroporphrinogen III synthase gene." Kawamukai M. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-372.
[5]	"Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase." Gotoh Y., Nishida E., Shimanuki M., Toda T., Imai Y., Yamamoto M. Mol. Cell. Biol. 13:6427-6434(1993) [PubMed: 8413241] [Abstract] Cited for: FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D31735 Genomic DNA. Translation: BAA06536.1. X57334 Genomic DNA. Translation: CAA40610.1. AB084886 mRNA. Translation: BAC54906.1. AB084887 mRNA. Translation: BAC54907.1. CU329670 Genomic DNA. Translation: CAB11693.1. AB004551 Genomic DNA. Translation: BAA20417.1.
PIR	S15663.
RefSeq	NP_594009.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P27638. 1 interaction.
STRING	P27638.
Genome annotation databases
GeneID	2542474.
GenomeReviews	Gene locus spk1 in contig CU329670_GR.
KEGG	spo:SPAC31G5.09c.
NMPDR	fig|4896.1.peg.3979.
Organism-specific databases
GeneDB_Spombe	SPAC31G5.09c.
Phylogenomic databases
OMA	WNKLFPK
OrthoDB	EOG9VQC5X
Enzyme and pathway databases
BRENDA	2.7.11.24. 653.
Gene expression databases
ArrayExpress	P27638.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR003527. MAP_kinase_CS. IPR008352. MAPK_p38. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01773. P38MAPKINASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS01351. MAPK. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPK1_SCHPO

Accession

Primary (citable) accession number: P27638

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	November 24, 2009
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)