Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mitogen-activated protein kinase spk1

Gene

spk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Involved in mating signal transduction pathway.1 Publication

Caution

Was originally thought to confer resistance to staurosporine.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by tyrosine and threonine phosphorylation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68ATPPROSITE-ProRule annotation1
Active sitei163Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 53ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • MAP kinase activity Source: PomBase

GO - Biological processi

  • MAPK cascade Source: PomBase
  • pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
  • positive regulation of conjugation with cellular fusion Source: GOC-OWL
  • regulation of ascospore formation Source: PomBase
  • regulation of gene expression Source: GO_Central

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.24 5613
ReactomeiR-SPO-110056 MAPK3 (ERK1) activation
R-SPO-112409 RAF-independent MAPK1/3 activation
R-SPO-112411 MAPK1 (ERK2) activation
R-SPO-198753 ERK/MAPK targets
R-SPO-202670 ERKs are inactivated
R-SPO-3371453 Regulation of HSF1-mediated heat shock response
R-SPO-445144 Signal transduction by L1
R-SPO-450341 Activation of the AP-1 family of transcription factors
R-SPO-5673001 RAF/MAP kinase cascade
R-SPO-5674135 MAP2K and MAPK activation
R-SPO-5674499 Negative feedback regulation of MAPK pathway
R-SPO-6798695 Neutrophil degranulation

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase spk1 (EC:2.7.11.24)
Short name:
MAP kinase spk1
Short name:
MAPK
Gene namesi
Name:spk1
ORF Names:SPAC31G5.09c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC31G5.09c
PomBaseiSPAC31G5.09c spk1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863401 – 372Mitogen-activated protein kinase spk1Add BLAST372

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei199PhosphothreonineBy similarity1
Modified residuei201PhosphotyrosineBy similarity1

Post-translational modificationi

Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27638
PaxDbiP27638
PRIDEiP27638

PTM databases

iPTMnetiP27638

Interactioni

Protein-protein interaction databases

BioGridi278934, 12 interactors
IntActiP27638, 3 interactors
STRINGi4896.SPAC31G5.09c.1

Structurei

3D structure databases

ProteinModelPortaliP27638
SMRiP27638
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 327Protein kinasePROSITE-ProRule annotationAdd BLAST289

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi199 – 201TXY3

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000233024
InParanoidiP27638
KOiK04371
OMAiMSSGAMD
OrthoDBiEOG092C2FL8
PhylomeDBiP27638

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008352 MAPK_p38-like
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01773 P38MAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P27638-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY
60 70 80 90 100
GVVCAALHKP SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI
110 120 130 140 150
LDILPPPSYQ ELEDVYIVQE LMETDLYRVI RSQPLSDDHC QYFTYQILRA
160 170 180 190 200
LKAMHSAGVV HRDLKPSNLL LNANCDLKVA DFGLARSTTA QGGNPGFMTE
210 220 230 240 250
YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP LFPGKDYHSQ
260 270 280 290 300
ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA
310 320 330 340 350
IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY
360 370
CNKEDLEIPV LKALIFREVN FR
Length:372
Mass (Da):42,003
Last modified:August 1, 1992 - v1
Checksum:i85D980514C9386C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31735 Genomic DNA Translation: BAA06536.1
X57334 Genomic DNA Translation: CAA40610.1
AB084886 mRNA Translation: BAC54906.1
AB084887 mRNA Translation: BAC54907.1
CU329670 Genomic DNA Translation: CAB11693.1
AB004551 Genomic DNA Translation: BAA20417.1
PIRiS15663
RefSeqiNP_594009.1, NM_001019435.2

Genome annotation databases

EnsemblFungiiSPAC31G5.09c.1; SPAC31G5.09c.1:pep; SPAC31G5.09c
GeneIDi2542474
KEGGispo:SPAC31G5.09c

Similar proteinsi

Entry informationi

Entry nameiSPK1_SCHPO
AccessioniPrimary (citable) accession number: P27638
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: May 23, 2018
This is version 166 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health