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P27638

- SPK1_SCHPO

UniProt

P27638 - SPK1_SCHPO

Protein

Mitogen-activated protein kinase spk1

Gene

spk1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 131 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Involved in mating signal transduction pathway.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Magnesium.By similarity

    Enzyme regulationi

    Activated by tyrosine and threonine phosphorylation.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei68 – 681ATPPROSITE-ProRule annotation
    Active sitei163 – 1631Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi45 – 539ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: PomBase
    3. protein kinase activity Source: PomBase

    GO - Biological processi

    1. cell cycle arrest in response to pheromone Source: PomBase
    2. MAPK cascade Source: GOC
    3. pheromone-dependent signal transduction involved in conjugation with cellular fusion Source: PomBase
    4. signal transduction involved in conjugation with cellular fusion Source: PomBase

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.24. 5615.
    ReactomeiREACT_202608. ERK2 activation.
    REACT_202848. Signaling by FGFR.
    REACT_205526. ERK1 activation.
    REACT_213398. Regulation of HSF1-mediated heat shock response.
    REACT_224814. Signal transduction by L1.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase spk1 (EC:2.7.11.24)
    Short name:
    MAP kinase spk1
    Short name:
    MAPK
    Gene namesi
    Name:spk1
    ORF Names:SPAC31G5.09c
    OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
    Taxonomic identifieri284812 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
    ProteomesiUP000002485: Chromosome I

    Organism-specific databases

    PomBaseiSPAC31G5.09c.

    Subcellular locationi

    Nucleus
    Note: Mainly nuclear.

    GO - Cellular componenti

    1. cytosol Source: PomBase
    2. mitotic spindle pole body Source: PomBase
    3. nucleus Source: PomBase

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 372372Mitogen-activated protein kinase spk1PRO_0000186340Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei199 – 1991PhosphothreonineBy similarity
    Modified residuei201 – 2011PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-199 and Tyr-201, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27638.

    Interactioni

    Protein-protein interaction databases

    BioGridi278934. 5 interactions.
    IntActiP27638. 3 interactions.
    MINTiMINT-4688010.
    STRINGi4896.SPAC31G5.09c-1.

    Structurei

    3D structure databases

    ProteinModelPortaliP27638.
    SMRiP27638. Positions 31-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 327289Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi199 – 2013TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    KOiK04371.
    OMAiHENIISV.
    OrthoDBiEOG7K3TWD.
    PhylomeDBiP27638.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01773. P38MAPKINASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P27638-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASATSTPTI ADGNSNKESV ATSRSPHTHD LNFELPEEYE MINLIGQGAY    50
    GVVCAALHKP SGLKVAVKKI HPFNHPVFCL RTLREIKLLR HFRHENIISI 100
    LDILPPPSYQ ELEDVYIVQE LMETDLYRVI RSQPLSDDHC QYFTYQILRA 150
    LKAMHSAGVV HRDLKPSNLL LNANCDLKVA DFGLARSTTA QGGNPGFMTE 200
    YVATRWYRAP EIMLSFREYS KAIDLWSTGC ILAEMLSARP LFPGKDYHSQ 250
    ITLILNILGT PTMDDFSRIK SARARKYIKS LPFTPKVSFK ALFPQASPDA 300
    IDLLEKLLTF NPDKRITAEE ALKHPYVAAY HDASDEPTAS PMPPNLVDLY 350
    CNKEDLEIPV LKALIFREVN FR 372
    Length:372
    Mass (Da):42,003
    Last modified:August 1, 1992 - v1
    Checksum:i85D980514C9386C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31735 Genomic DNA. Translation: BAA06536.1.
    X57334 Genomic DNA. Translation: CAA40610.1.
    AB084886 mRNA. Translation: BAC54906.1.
    AB084887 mRNA. Translation: BAC54907.1.
    CU329670 Genomic DNA. Translation: CAB11693.1.
    AB004551 Genomic DNA. Translation: BAA20417.1.
    PIRiS15663.
    RefSeqiNP_594009.1. NM_001019435.2.

    Genome annotation databases

    EnsemblFungiiSPAC31G5.09c.1; SPAC31G5.09c.1:pep; SPAC31G5.09c.
    GeneIDi2542474.
    KEGGispo:SPAC31G5.09c.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    D31735 Genomic DNA. Translation: BAA06536.1 .
    X57334 Genomic DNA. Translation: CAA40610.1 .
    AB084886 mRNA. Translation: BAC54906.1 .
    AB084887 mRNA. Translation: BAC54907.1 .
    CU329670 Genomic DNA. Translation: CAB11693.1 .
    AB004551 Genomic DNA. Translation: BAA20417.1 .
    PIRi S15663.
    RefSeqi NP_594009.1. NM_001019435.2.

    3D structure databases

    ProteinModelPortali P27638.
    SMRi P27638. Positions 31-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 278934. 5 interactions.
    IntActi P27638. 3 interactions.
    MINTi MINT-4688010.
    STRINGi 4896.SPAC31G5.09c-1.

    Proteomic databases

    MaxQBi P27638.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii SPAC31G5.09c.1 ; SPAC31G5.09c.1:pep ; SPAC31G5.09c .
    GeneIDi 2542474.
    KEGGi spo:SPAC31G5.09c.

    Organism-specific databases

    PomBasei SPAC31G5.09c.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    KOi K04371.
    OMAi HENIISV.
    OrthoDBi EOG7K3TWD.
    PhylomeDBi P27638.

    Enzyme and pathway databases

    BRENDAi 2.7.11.24. 5615.
    Reactomei REACT_202608. ERK2 activation.
    REACT_202848. Signaling by FGFR.
    REACT_205526. ERK1 activation.
    REACT_213398. Regulation of HSF1-mediated heat shock response.
    REACT_224814. Signal transduction by L1.

    Miscellaneous databases

    NextBioi 20803529.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR008352. MAPK_p38.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01773. P38MAPKINASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Fission yeast genes that confer resistance to staurosporine encode an AP-1-like transcription factor and a protein kinase related to the mammalian ERK1/MAP2 and budding yeast FUS3 and KSS1 kinases."
      Toda T., Shimanuki M., Yanagida M.
      Genes Dev. 5:60-73(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of abundant polyA plus non-coding RNAs that are expressed in Schizosaccharomyces pombe."
      Watanabe T., Saito T.T., Nabeshima K., Nojima H.
      Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: CD16-1.
    3. "The genome sequence of Schizosaccharomyces pombe."
      Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
      , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
      Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 972 / ATCC 24843.
    4. "S.pombe uroporphrinogen III synthase gene."
      Kawamukai M.
      Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 286-372.
    5. "Schizosaccharomyces pombe Spk1 is a tyrosine-phosphorylated protein functionally related to Xenopus mitogen-activated protein kinase."
      Gotoh Y., Nishida E., Shimanuki M., Toda T., Imai Y., Yamamoto M.
      Mol. Cell. Biol. 13:6427-6434(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiSPK1_SCHPO
    AccessioniPrimary (citable) accession number: P27638
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 131 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Caution

    Was originally thought to confer resistance to staurosporine.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Schizosaccharomyces pombe
      Schizosaccharomyces pombe: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3