Bud site selection protein 14 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Contribute

Send feedback

Read comments (?) or add your own

P27637 (BUD14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 101. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

text xml rdf/xml gff fasta

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Bud site selection protein 14

Gene names

Name:	BUD14
Ordered Locus Names:	YAR014C
ORF Names:	FUN2

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	709 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Important for bud site selection. Seems to be a regulatory subunit of the BUD14-GLC7 type-I phosphatase complex. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex. Ref.6 Ref.9
Subunit structure	Interacts with GLC7. Ref.7 Ref.9
Miscellaneous	Present with 538 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Contains 1 SH3 domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Domain	SH3 domain
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cytoskeleton organization Inferred from physical interaction. Source: SGD negative regulation of actin filament polymerization Inferred from mutant phenotype. Source: SGD regulation of cell shape during vegetative growth phase Inferred from genetic interaction Ref.7. Source: SGD regulation of protein localization Inferred from direct assay. Source: SGD regulation of transcription, DNA-dependent Inferred from mutant phenotype. Source: SGD
Cellular component	cellular bud neck Inferred from direct assay Ref.6. Source: SGD cellular bud tip Inferred from direct assay Ref.6. Source: SGD incipient cellular bud site Inferred from direct assay Ref.6. Source: SGD
Molecular function	protein binding Inferred from physical interaction. Source: IntAct protein phosphatase type 1 regulator activity Inferred from physical interaction. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
BEM2	P39960	3	EBI-20747,EBI-3517
GLC7	P32598	7	EBI-20747,EBI-13715

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 707

707

Bud site selection protein 14

PRO_0000065012

Regions

Domain

259 – 320

SH3

Amino acid modifications

Modified residue

Phosphoserine Ref.12

Modified residue

160

Phosphoserine Ref.11 Ref.12

Modified residue

162

Phosphoserine Ref.12

Modified residue

212

Phosphoserine Ref.10

Modified residue

222

Phosphoserine Ref.10 Ref.12

Modified residue

360

Phosphoserine Ref.12

Modified residue

376

Phosphoserine Ref.12

Modified residue

378

Phosphoserine Ref.12

Modified residue

398

Phosphoserine Ref.12

Modified residue

401

Phosphoserine Ref.12

Modified residue

641

Phosphoserine Ref.12

Modified residue

642

Phosphoserine Ref.12

Modified residue

655

Phosphoserine Ref.12

Modified residue

658

Phosphoserine Ref.12

Modified residue

672

Phosphothreonine Ref.12

Experimental info

Sequence conflict

439

G → A no nucleotide entry Ref.1

Sequence conflict

439

G → A in AAC04962. Ref.2

Sequence conflict

573 – 595

KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R no nucleotide entry Ref.1

Sequence conflict

573 – 595

KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R in AAC04962. Ref.2

Sequence conflict

609

A → P no nucleotide entry Ref.1

Sequence conflict

609

A → P in AAC04962. Ref.2

Sequences

Sequence

Length

Mass (Da)

Tools

P27637 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: F123511A4C592EB4
Show »

FASTA

709

78,981

        10         20         30         40         50         60 
MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL NDPTLIEDYS 

        70         80         90        100        110        120 
DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN SKLNILSNAA EKGHDVLRNR 

       130        140        150        160        170        180 
DDDKELEEEN VEKHMHSNSK RDQRHYKENS SELPDSYDYS DSEFEDNLER RLQEIETDSV 

       190        200        210        220        230        240 
DSADKDEVHF SVNNTMNPDV DDFSDGLKYA ISEDEDEEEN YSDDDDFDRK FQDSGFQGEK 

       250        260        270        280        290        300 
DDLEEENDDY QPLSPPRELD PDKLYALYAF NGHDSSHCQL GQDEPCILLN DQDAYWWLVK 

       310        320        330        340        350        360 
RITDGKIGFA PAEILETFPE RLARLNCWKN ENMSSQSVAS SDSKDDSISS GNKNQSDAES 

       370        380        390        400        410        420 
IIPTPALNGY GKGNKSVSFN DVVGYADRFI DDAIEDTSLD SNDDGGEGNG QSYDDDVDND 

       430        440        450        460        470        480 
KETKVTHRDE YTEAKLNFGK FQDDDTSDVV SDVSFSTSLN TPLNVKKVRR QDNKNESEPK 

       490        500        510        520        530        540 
TSSSKDREDD YNANRYVGQE KSEPVDSDYD TDLKKVFEAP RMPFANGMAK SDSQNSLSTI 

       550        560        570        580        590        600 
GEFSPSSSEW TNESPSTPIV EESSSIPSSR AIKDISQYIH AKSKIEETTN VENTEGQIQA 

       610        620        630        640        650        660 
SLGSSGGMAN QTDAEQPKEE LEKHHSTPEE EKQSTLSLHS SSEEDFYMDE QRAVSSASIN 

       670        680        690        700 
SSLSGSRALS NTNMSDPASK PNSLVQHLYA PVFDRMDVLM KQLDEIIRK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Fisk D., Cherry J.M. Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 439; 573-595 AND 609. Strain: ATCC 204508 / S288c.
[5]	"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I." Davies C.J., Hutchison C.A. III Nucleic Acids Res. 19:5731-5738(1991) [PubMed: 1658741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
[6]	"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae." Ni L., Snyder M. Mol. Biol. Cell 12:2147-2170(2001) [PubMed: 11452010] [Abstract] Cited for: FUNCTION.
[7]	"The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae." Cullen P.J., Sprague G.F. Jr. Eukaryot. Cell 1:884-894(2002) [PubMed: 12477789] [Abstract] Cited for: INTERACTION WITH GLC7.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast." Knaus M., Cameroni E., Pedruzzi I., Tatchell K., De Virgilio C., Peter M. EMBO J. 24:3000-3011(2005) [PubMed: 16107882] [Abstract] Cited for: FUNCTION, INTERACTION WITH GLC7, FUNCTION OF THE BUD14-GLC7 COMPLEX.
[10]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-222 AND SER-656, MASS SPECTROMETRY. Strain: ADR376.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-97; SER-160; SER-162; SER-222; SER-360; SER-376; SER-378; SER-398; SER-401; SER-641; SER-642; SER-655; SER-658 AND THR-672, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L22015 Genomic DNA. Translation: AAC04962.2. M67445 Genomic DNA. Translation: AAA34397.1. BK006935 Genomic DNA. Translation: DAA06993.2.
PIR	S40904.
RefSeq	NP_009408.3. NM_001178215.2.
3D structure databases
ProteinModelPortal	P27637.
SMR	P27637. Positions 251-317.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1756N.
IntAct	P27637. 51 interactions.
MINT	MINT-403701.
STRING	P27637.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	851271.
KEGG	sce:YAR014C.
NMPDR	fig\|4932.3.peg.92.
Organism-specific databases
CYGD	YAR014c.
SGD	S000000069. BUD14.
Phylogenomic databases
eggNOG	fuNOG06503.
GeneTree	EFGT00050000002830.
HOGENOM	HBG203640.
OMA	NCWKNEN.
OrthoDB	EOG4R7ZM8.
Gene expression databases
ArrayExpress	P27637.
Genevestigator	P27637.
GermOnline	YAR014C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001452. SH3_domain. [Graphical view]
Pfam	PF00018. SH3_1. 1 hit. [Graphical view]
SMART	SM00326. SH3. 1 hit. [Graphical view]
SUPFAM	SSF50044. SH3. 1 hit.
PROSITE	PS50002. SH3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968248.

Entry information

Entry name

BUD14_YEAST

Accession

Primary (citable) accession number: P27637
Secondary accession number(s): D6VPM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 101 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents