Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bud site selection protein 14

Gene

BUD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Important for bud site selection. Seems to be a regulatory subunit of the BUD14-GLC7 type-I phosphatase complex. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex.2 Publications

GO - Molecular functioni

  • protein phosphatase type 1 regulator activity Source: SGD

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • cytoskeleton organization Source: SGD
  • negative regulation of actin filament polymerization Source: SGD
  • regulation of cell shape during vegetative growth phase Source: SGD
  • regulation of cytokinesis Source: SGD
  • regulation of formin-nucleated actin cable assembly Source: SGD
  • regulation of protein localization Source: SGD
  • regulation of transcription, DNA-templated Source: SGD
  • regulation of vesicle-mediated transport Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division

Enzyme and pathway databases

BioCyciYEAST:G3O-28873-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Bud site selection protein 14
Gene namesi
Name:BUD14
Ordered Locus Names:YAR014C
ORF Names:FUN2
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAR014C.
SGDiS000000069. BUD14.

Subcellular locationi

GO - Cellular componenti

  • cellular bud neck Source: SGD
  • cellular bud tip Source: SGD
  • incipient cellular bud site Source: SGD
  • Kelch-containing formin regulatory complex Source: SGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000650122 – 707Bud site selection protein 14Add BLAST706

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei159PhosphotyrosineCombined sources1
Modified residuei160PhosphoserineCombined sources1
Modified residuei162PhosphoserineCombined sources1
Modified residuei177PhosphothreonineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei376PhosphoserineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei401PhosphoserineCombined sources1
Modified residuei507PhosphoserineCombined sources1
Modified residuei655PhosphoserineCombined sources1
Modified residuei658PhosphoserineCombined sources1
Modified residuei670PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP27637.
PRIDEiP27637.

PTM databases

iPTMnetiP27637.

Interactioni

Subunit structurei

Interacts with GLC7.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-20747,EBI-20747
BEM2P399603EBI-20747,EBI-3517
GLC7P325987EBI-20747,EBI-13715
KIN2P131863EBI-20747,EBI-9723

Protein-protein interaction databases

BioGridi31798. 171 interactors.
DIPiDIP-1756N.
IntActiP27637. 98 interactors.
MINTiMINT-403701.

Structurei

3D structure databases

ProteinModelPortaliP27637.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini259 – 320SH3PROSITE-ProRule annotationAdd BLAST62

Sequence similaritiesi

Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH3 domain

Phylogenomic databases

HOGENOMiHOG000095277.
InParanoidiP27637.
OMAiNCWKNEN.
OrthoDBiEOG092C0T40.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27637-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL
60 70 80 90 100
NDPTLIEDYS DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN
110 120 130 140 150
SKLNILSNAA EKGHDVLRNR DDDKELEEEN VEKHMHSNSK RDQRHYKENS
160 170 180 190 200
SELPDSYDYS DSEFEDNLER RLQEIETDSV DSADKDEVHF SVNNTMNPDV
210 220 230 240 250
DDFSDGLKYA ISEDEDEEEN YSDDDDFDRK FQDSGFQGEK DDLEEENDDY
260 270 280 290 300
QPLSPPRELD PDKLYALYAF NGHDSSHCQL GQDEPCILLN DQDAYWWLVK
310 320 330 340 350
RITDGKIGFA PAEILETFPE RLARLNCWKN ENMSSQSVAS SDSKDDSISS
360 370 380 390 400
GNKNQSDAES IIPTPALNGY GKGNKSVSFN DVVGYADRFI DDAIEDTSLD
410 420 430 440 450
SNDDGGEGNG QSYDDDVDND KETKVTHRDE YTEAKLNFGK FQDDDTSDVV
460 470 480 490 500
SDVSFSTSLN TPLNVKKVRR QDNKNESEPK TSSSKDREDD YNANRYVGQE
510 520 530 540 550
KSEPVDSDYD TDLKKVFEAP RMPFANGMAK SDSQNSLSTI GEFSPSSSEW
560 570 580 590 600
TNESPSTPIV EESSSIPSSR AIKDISQYIH AKSKIEETTN VENTEGQIQA
610 620 630 640 650
SLGSSGGMAN QTDAEQPKEE LEKHHSTPEE EKQSTLSLHS SSEEDFYMDE
660 670 680 690 700
QRAVSSASIN SSLSGSRALS NTNMSDPASK PNSLVQHLYA PVFDRMDVLM

KQLDEIIRK
Length:709
Mass (Da):78,981
Last modified:July 27, 2011 - v4
Checksum:iF123511A4C592EB4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti439G → A no nucleotide entry (PubMed:7941740).Curated1
Sequence conflicti439G → A in AAC04962 (PubMed:7731988).Curated1
Sequence conflicti573 – 595KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R no nucleotide entry (PubMed:7941740).CuratedAdd BLAST23
Sequence conflicti573 – 595KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R in AAC04962 (PubMed:7731988).CuratedAdd BLAST23
Sequence conflicti609A → P no nucleotide entry (PubMed:7941740).Curated1
Sequence conflicti609A → P in AAC04962 (PubMed:7731988).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22015 Genomic DNA. Translation: AAC04962.2.
M67445 Genomic DNA. Translation: AAA34397.1.
BK006935 Genomic DNA. Translation: DAA06993.2.
PIRiS40904.
RefSeqiNP_009408.3. NM_001178215.2.

Genome annotation databases

EnsemblFungiiYAR014C; YAR014C; YAR014C.
GeneIDi851271.
KEGGisce:YAR014C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22015 Genomic DNA. Translation: AAC04962.2.
M67445 Genomic DNA. Translation: AAA34397.1.
BK006935 Genomic DNA. Translation: DAA06993.2.
PIRiS40904.
RefSeqiNP_009408.3. NM_001178215.2.

3D structure databases

ProteinModelPortaliP27637.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31798. 171 interactors.
DIPiDIP-1756N.
IntActiP27637. 98 interactors.
MINTiMINT-403701.

PTM databases

iPTMnetiP27637.

Proteomic databases

MaxQBiP27637.
PRIDEiP27637.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAR014C; YAR014C; YAR014C.
GeneIDi851271.
KEGGisce:YAR014C.

Organism-specific databases

EuPathDBiFungiDB:YAR014C.
SGDiS000000069. BUD14.

Phylogenomic databases

HOGENOMiHOG000095277.
InParanoidiP27637.
OMAiNCWKNEN.
OrthoDBiEOG092C0T40.

Enzyme and pathway databases

BioCyciYEAST:G3O-28873-MONOMER.

Miscellaneous databases

PROiP27637.

Family and domain databases

InterProiIPR001452. SH3_domain.
[Graphical view]
PfamiPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTiSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
PROSITEiPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiBUD14_YEAST
AccessioniPrimary (citable) accession number: P27637
Secondary accession number(s): D6VPM3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 150 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.