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P27637

- BUD14_YEAST

UniProt

P27637 - BUD14_YEAST

Protein

Bud site selection protein 14

Gene

BUD14

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 4 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Important for bud site selection. Seems to be a regulatory subunit of the BUD14-GLC7 type-I phosphatase complex. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex.2 Publications

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. protein phosphatase type 1 regulator activity Source: SGD

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. cytoskeleton organization Source: SGD
    4. negative regulation of actin filament polymerization Source: SGD
    5. regulation of catalytic activity Source: GOC
    6. regulation of cell shape during vegetative growth phase Source: SGD
    7. regulation of protein localization Source: SGD
    8. regulation of transcription, DNA-templated Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28873-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Bud site selection protein 14
    Gene namesi
    Name:BUD14
    Ordered Locus Names:YAR014C
    ORF Names:FUN2
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAR014c.
    SGDiS000000069. BUD14.

    Subcellular locationi

    GO - Cellular componenti

    1. cellular bud neck Source: SGD
    2. cellular bud tip Source: SGD
    3. incipient cellular bud site Source: SGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 707706Bud site selection protein 14PRO_0000065012Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine1 Publication
    Modified residuei159 – 1591Phosphotyrosine1 Publication
    Modified residuei160 – 1601Phosphoserine2 Publications
    Modified residuei162 – 1621Phosphoserine1 Publication
    Modified residuei177 – 1771Phosphothreonine1 Publication
    Modified residuei212 – 2121Phosphoserine2 Publications
    Modified residuei222 – 2221Phosphoserine2 Publications
    Modified residuei376 – 3761Phosphoserine2 Publications
    Modified residuei378 – 3781Phosphoserine2 Publications
    Modified residuei401 – 4011Phosphoserine1 Publication
    Modified residuei507 – 5071Phosphoserine1 Publication
    Modified residuei655 – 6551Phosphoserine1 Publication
    Modified residuei658 – 6581Phosphoserine2 Publications
    Modified residuei670 – 6701Phosphoserine1 Publication

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP27637.
    PaxDbiP27637.

    Expressioni

    Gene expression databases

    GenevestigatoriP27637.

    Interactioni

    Subunit structurei

    Interacts with GLC7.2 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BEM2P399603EBI-20747,EBI-3517
    GLC7P325987EBI-20747,EBI-13715
    KIN2P131863EBI-20747,EBI-9723

    Protein-protein interaction databases

    BioGridi31798. 170 interactions.
    DIPiDIP-1756N.
    IntActiP27637. 96 interactions.
    MINTiMINT-403701.
    STRINGi4932.YAR014C.

    Structurei

    3D structure databases

    ProteinModelPortaliP27637.
    SMRiP27637. Positions 263-317.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini259 – 32062SH3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 SH3 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH3 domain

    Phylogenomic databases

    eggNOGiNOG291368.
    HOGENOMiHOG000095277.
    OMAiNCWKNEN.
    OrthoDBiEOG7MSMZD.

    Family and domain databases

    InterProiIPR001452. SH3_domain.
    [Graphical view]
    PfamiPF00018. SH3_1. 1 hit.
    [Graphical view]
    SMARTiSM00326. SH3. 1 hit.
    [Graphical view]
    SUPFAMiSSF50044. SSF50044. 1 hit.
    PROSITEiPS50002. SH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27637-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL    50
    NDPTLIEDYS DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN 100
    SKLNILSNAA EKGHDVLRNR DDDKELEEEN VEKHMHSNSK RDQRHYKENS 150
    SELPDSYDYS DSEFEDNLER RLQEIETDSV DSADKDEVHF SVNNTMNPDV 200
    DDFSDGLKYA ISEDEDEEEN YSDDDDFDRK FQDSGFQGEK DDLEEENDDY 250
    QPLSPPRELD PDKLYALYAF NGHDSSHCQL GQDEPCILLN DQDAYWWLVK 300
    RITDGKIGFA PAEILETFPE RLARLNCWKN ENMSSQSVAS SDSKDDSISS 350
    GNKNQSDAES IIPTPALNGY GKGNKSVSFN DVVGYADRFI DDAIEDTSLD 400
    SNDDGGEGNG QSYDDDVDND KETKVTHRDE YTEAKLNFGK FQDDDTSDVV 450
    SDVSFSTSLN TPLNVKKVRR QDNKNESEPK TSSSKDREDD YNANRYVGQE 500
    KSEPVDSDYD TDLKKVFEAP RMPFANGMAK SDSQNSLSTI GEFSPSSSEW 550
    TNESPSTPIV EESSSIPSSR AIKDISQYIH AKSKIEETTN VENTEGQIQA 600
    SLGSSGGMAN QTDAEQPKEE LEKHHSTPEE EKQSTLSLHS SSEEDFYMDE 650
    QRAVSSASIN SSLSGSRALS NTNMSDPASK PNSLVQHLYA PVFDRMDVLM 700
    KQLDEIIRK 709
    Length:709
    Mass (Da):78,981
    Last modified:July 27, 2011 - v4
    Checksum:iF123511A4C592EB4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti439 – 4391G → A no nucleotide entry (PubMed:7941740)Curated
    Sequence conflicti439 – 4391G → A in AAC04962. (PubMed:7731988)Curated
    Sequence conflicti573 – 59523KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R no nucleotide entry (PubMed:7941740)CuratedAdd
    BLAST
    Sequence conflicti573 – 59523KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R in AAC04962. (PubMed:7731988)CuratedAdd
    BLAST
    Sequence conflicti609 – 6091A → P no nucleotide entry (PubMed:7941740)Curated
    Sequence conflicti609 – 6091A → P in AAC04962. (PubMed:7731988)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04962.2.
    M67445 Genomic DNA. Translation: AAA34397.1.
    BK006935 Genomic DNA. Translation: DAA06993.2.
    PIRiS40904.
    RefSeqiNP_009408.3. NM_001178215.2.

    Genome annotation databases

    EnsemblFungiiYAR014C; YAR014C; YAR014C.
    GeneIDi851271.
    KEGGisce:YAR014C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L22015 Genomic DNA. Translation: AAC04962.2 .
    M67445 Genomic DNA. Translation: AAA34397.1 .
    BK006935 Genomic DNA. Translation: DAA06993.2 .
    PIRi S40904.
    RefSeqi NP_009408.3. NM_001178215.2.

    3D structure databases

    ProteinModelPortali P27637.
    SMRi P27637. Positions 263-317.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31798. 170 interactions.
    DIPi DIP-1756N.
    IntActi P27637. 96 interactions.
    MINTi MINT-403701.
    STRINGi 4932.YAR014C.

    Proteomic databases

    MaxQBi P27637.
    PaxDbi P27637.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR014C ; YAR014C ; YAR014C .
    GeneIDi 851271.
    KEGGi sce:YAR014C.

    Organism-specific databases

    CYGDi YAR014c.
    SGDi S000000069. BUD14.

    Phylogenomic databases

    eggNOGi NOG291368.
    HOGENOMi HOG000095277.
    OMAi NCWKNEN.
    OrthoDBi EOG7MSMZD.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28873-MONOMER.

    Miscellaneous databases

    NextBioi 968248.

    Gene expression databases

    Genevestigatori P27637.

    Family and domain databases

    InterProi IPR001452. SH3_domain.
    [Graphical view ]
    Pfami PF00018. SH3_1. 1 hit.
    [Graphical view ]
    SMARTi SM00326. SH3. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50044. SSF50044. 1 hit.
    PROSITEi PS50002. SH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
      Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Fisk D., Cherry J.M.
      Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    4. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 439; 573-595 AND 609.
      Strain: ATCC 204508 / S288c.
    5. "A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
      Davies C.J., Hutchison C.A. III
      Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
    6. "A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
      Ni L., Snyder M.
      Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    7. "The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae."
      Cullen P.J., Sprague G.F. Jr.
      Eukaryot. Cell 1:884-894(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH GLC7.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast."
      Knaus M., Cameroni E., Pedruzzi I., Tatchell K., De Virgilio C., Peter M.
      EMBO J. 24:3000-3011(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INTERACTION WITH GLC7, FUNCTION OF THE BUD14-GLC7 COMPLEX.
    10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-222 AND SER-658, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160; SER-376 AND SER-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-159; SER-160; SER-162; THR-177; SER-212; SER-222; SER-376; SER-378; SER-401; SER-507; SER-655; SER-658 AND SER-670, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    13. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBUD14_YEAST
    AccessioniPrimary (citable) accession number: P27637
    Secondary accession number(s): D6VPM3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 130 of the entry and version 4 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 538 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3