Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P27637 (BUD14_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bud site selection protein 14
Gene names
Name:BUD14
Ordered Locus Names:YAR014C
ORF Names:FUN2
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length709 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Important for bud site selection. Seems to be a regulatory subunit of the BUD14-GLC7 type-I phosphatase complex. The BUD14-GLC7 complex is necessary to regulate microtubule dynamics at the cortex and may function as a specific activator of the dynein complex. Ref.6 Ref.9

Subunit structure

Interacts with GLC7. Ref.7 Ref.9

Miscellaneous

Present with 538 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 SH3 domain.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.13
Chain2 – 707706Bud site selection protein 14
PRO_0000065012

Regions

Domain259 – 32062SH3

Amino acid modifications

Modified residue21N-acetylserine Ref.13
Modified residue1591Phosphotyrosine Ref.12
Modified residue1601Phosphoserine Ref.11 Ref.12
Modified residue1621Phosphoserine Ref.12
Modified residue1771Phosphothreonine Ref.12
Modified residue2121Phosphoserine Ref.10 Ref.12
Modified residue2221Phosphoserine Ref.10 Ref.12
Modified residue3761Phosphoserine Ref.11 Ref.12
Modified residue3781Phosphoserine Ref.11 Ref.12
Modified residue4011Phosphoserine Ref.12
Modified residue5071Phosphoserine Ref.12
Modified residue6551Phosphoserine Ref.12
Modified residue6581Phosphoserine Ref.10 Ref.12
Modified residue6701Phosphoserine Ref.12

Experimental info

Sequence conflict4391G → A no nucleotide entry Ref.1
Sequence conflict4391G → A in AAC04962. Ref.2
Sequence conflict573 – 59523KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R no nucleotide entry Ref.1
Sequence conflict573 – 59523KDISQ…VENTE → RTFTYIMQNRKLRDNKRGKH R in AAC04962. Ref.2
Sequence conflict6091A → P no nucleotide entry Ref.1
Sequence conflict6091A → P in AAC04962. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P27637 [UniParc].

Last modified July 27, 2011. Version 4.
Checksum: F123511A4C592EB4

FASTA70978,981
        10         20         30         40         50         60 
MSNKEEHVDE TSASGVKEVS SIAARHDNGY APSLITSTSG MDSFQSHALL NDPTLIEDYS 

        70         80         90        100        110        120 
DIINNRPTSG SKLTLGNEDS ESMGGSVVVT PTSNKSSPFN SKLNILSNAA EKGHDVLRNR 

       130        140        150        160        170        180 
DDDKELEEEN VEKHMHSNSK RDQRHYKENS SELPDSYDYS DSEFEDNLER RLQEIETDSV 

       190        200        210        220        230        240 
DSADKDEVHF SVNNTMNPDV DDFSDGLKYA ISEDEDEEEN YSDDDDFDRK FQDSGFQGEK 

       250        260        270        280        290        300 
DDLEEENDDY QPLSPPRELD PDKLYALYAF NGHDSSHCQL GQDEPCILLN DQDAYWWLVK 

       310        320        330        340        350        360 
RITDGKIGFA PAEILETFPE RLARLNCWKN ENMSSQSVAS SDSKDDSISS GNKNQSDAES 

       370        380        390        400        410        420 
IIPTPALNGY GKGNKSVSFN DVVGYADRFI DDAIEDTSLD SNDDGGEGNG QSYDDDVDND 

       430        440        450        460        470        480 
KETKVTHRDE YTEAKLNFGK FQDDDTSDVV SDVSFSTSLN TPLNVKKVRR QDNKNESEPK 

       490        500        510        520        530        540 
TSSSKDREDD YNANRYVGQE KSEPVDSDYD TDLKKVFEAP RMPFANGMAK SDSQNSLSTI 

       550        560        570        580        590        600 
GEFSPSSSEW TNESPSTPIV EESSSIPSSR AIKDISQYIH AKSKIEETTN VENTEGQIQA 

       610        620        630        640        650        660 
SLGSSGGMAN QTDAEQPKEE LEKHHSTPEE EKQSTLSLHS SSEEDFYMDE QRAVSSASIN 

       670        680        690        700 
SSLSGSRALS NTNMSDPASK PNSLVQHLYA PVFDRMDVLM KQLDEIIRK 

« Hide

References

« Hide 'large scale' references
[1]"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Fisk D., Cherry J.M.
Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 439; 573-595 AND 609.
Strain: ATCC 204508 / S288c.
[5]"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
Davies C.J., Hutchison C.A. III
Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-142.
[6]"A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae."
Ni L., Snyder M.
Mol. Biol. Cell 12:2147-2170(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[7]"The Glc7p-interacting protein Bud14p attenuates polarized growth, pheromone response, and filamentous growth in Saccharomyces cerevisiae."
Cullen P.J., Sprague G.F. Jr.
Eukaryot. Cell 1:884-894(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH GLC7.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"The Bud14p-Glc7p complex functions as a cortical regulator of dynein in budding yeast."
Knaus M., Cameroni E., Pedruzzi I., Tatchell K., De Virgilio C., Peter M.
EMBO J. 24:3000-3011(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH GLC7, FUNCTION OF THE BUD14-GLC7 COMPLEX.
[10]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-212; SER-222 AND SER-658, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[11]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-160; SER-376 AND SER-378, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-159; SER-160; SER-162; THR-177; SER-212; SER-222; SER-376; SER-378; SER-401; SER-507; SER-655; SER-658 AND SER-670, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[13]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L22015 Genomic DNA. Translation: AAC04962.2.
M67445 Genomic DNA. Translation: AAA34397.1.
BK006935 Genomic DNA. Translation: DAA06993.2.
PIRS40904.
RefSeqNP_009408.3. NM_001178215.2.

3D structure databases

ProteinModelPortalP27637.
SMRP27637. Positions 262-316.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31798. 167 interactions.
DIPDIP-1756N.
IntActP27637. 96 interactions.
MINTMINT-403701.
STRING4932.YAR014C.

Proteomic databases

PaxDbP27637.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAR014C; YAR014C; YAR014C.
GeneID851271.
KEGGsce:YAR014C.

Organism-specific databases

CYGDYAR014c.
SGDS000000069. BUD14.

Phylogenomic databases

eggNOGNOG291368.
HOGENOMHOG000095277.
OMANCWKNEN.
OrthoDBEOG7MSMZD.

Enzyme and pathway databases

BioCycYEAST:G3O-28873-MONOMER.

Gene expression databases

GenevestigatorP27637.

Family and domain databases

InterProIPR001452. SH3_domain.
[Graphical view]
PfamPF00018. SH3_1. 1 hit.
[Graphical view]
SMARTSM00326. SH3. 1 hit.
[Graphical view]
SUPFAMSSF50044. SSF50044. 1 hit.
PROSITEPS50002. SH3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968248.

Entry information

Entry nameBUD14_YEAST
AccessionPrimary (citable) accession number: P27637
Secondary accession number(s): D6VPM3
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: March 19, 2014
This is version 125 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families