Cell division control protein 15 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P27636 (CDC15_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cell division control protein 15
EC=2.7.11.1

Gene names

Name:	CDC15
Ordered Locus Names:	YAR019C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	974 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Essential for late nuclear division in the mitotic cycle. Required for inactivation of CDC2 kinase activity at the end of mitosis.
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cytoplasm › cytoskeleton › spindle pole Ref.8.
Miscellaneous	Present with 238 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 1 protein kinase domain.
Caution	It is uncertain whether Met-1 or Met-4 is the initiator.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cytokinesis Inferred from mutant phenotype PubMed 11267871. Source: SGD meiotic anaphase II Inferred from mutant phenotype PubMed 17660551. Source: SGD mitosis Inferred from electronic annotation. Source: UniProtKB-KW regulation of exit from mitosis Inferred from mutant phenotype PubMed 11267871. Source: SGD
Cellular_component	cellular bud neck Inferred from direct assay PubMed 12832486. Source: SGD cytoplasm Inferred from direct assay PubMed 22842922. Source: SGD spindle pole body Inferred from direct assay PubMed 10662594. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein kinase activity Inferred from direct assay PubMed 11404483 PubMed 16319894. Source: SGD protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TEM1	P38987	6	EBI-4200,EBI-19113

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 974

974

Cell division control protein 15

PRO_0000085717

Regions

Domain

25 – 272

248

Protein kinase

Nucleotide binding

31 – 39

ATP By similarity

Sites

Active site

146

Proton acceptor By similarity

Binding site

ATP By similarity

Amino acid modifications

Modified residue

309

Phosphoserine Ref.12

Modified residue

560

Phosphoserine Ref.12

Modified residue

562

Phosphoserine Ref.10 Ref.12

Modified residue

564

Phosphothreonine Ref.12

Modified residue

596

Phosphoserine Ref.12

Modified residue

946

Phosphoserine Ref.11 Ref.12

Experimental info

Sequence conflict

316

A → R in CAA43041. Ref.1

Sequence conflict

316

A → R in CAA36906. Ref.2

Sequence conflict

316

A → R Ref.4

Sequence conflict

316

A → R in AAC04965. Ref.5

Sequence conflict

321

A → P in CAA43041. Ref.1

Sequence conflict

321

A → P in CAA36906. Ref.2

Sequence conflict

321

A → P Ref.4

Sequence conflict

321

A → P in AAC04965. Ref.5

Sequence conflict

900 – 902

KDV → NGC no nucleotide entry Ref.4

Sequence conflict

900 – 902

KDV → NGC in AAC04965. Ref.5

Sequences

Sequence

Length

Mass (Da)

Tools

P27636 [UniParc].

Last modified July 27, 2011. Version 3.
Checksum: 99383BD871A4A726
Show »

FASTA

974

110,242

        10         20         30         40         50         60 
MNSMADTDRV NLTPIQRASE KSVQYHLKQV IGRGSYGVVY KAINKHTDQV VAIKEVVYEN 

        70         80         90        100        110        120 
DEELNDIMAE ISLLKNLNHN NIVKYHGFIR KSYELYILLE YCANGSLRRL ISRSSTGLSE 

       130        140        150        160        170        180 
NESKTYVTQT LLGLKYLHGE GVIHRDIKAA NILLSADNTV KLADFGVSTI VNSSALTLAG 

       190        200        210        220        230        240 
TLNWMAPEIL GNRGASTLSD IWSLGATVVE MLTKNPPYHN LTDANIYYAV ENDTYYPPSS 

       250        260        270        280        290        300 
FSEPLKDFLS KCFVKNMYKR PTADQLLKHV WINSTENVKV DKLNKFKEDF TDADYHWDAD 

       310        320        330        340        350        360 
FQEEKLNISP SKFSLAAAPA AWAENNQELD LMPPTESQLL SQLKSSSKPL TDLHVLFSVC 

       370        380        390        400        410        420 
SLENIADTII ECLSRTTVDK RLITAFGSIF VYDTQHNHSR LRLKFIAMGG IPLIIKFEHL 

       430        440        450        460        470        480 
AKEFVIDYPQ TLIECGIMYP PNFASLKTPK YILELVYRFY DLTSTAFWCR WCFKHLDISL 

       490        500        510        520        530        540 
LLNNIHERRA QSILLKLSSY APWSFEKILP SLIDSKLKKK ILISPQITYV VFKSINYMIT 

       550        560        570        580        590        600 
TNDDKIHKSA IPSSSSLPLS SSPTRNSPVN SVQSPSRSPV HSLMATRPSS PMRHKSISNF 

       610        620        630        640        650        660 
PHLTISSKSR LLIELPEGFF TWLTSFFVDM AQIKDLSVLK YFTKLCYLTV HINSTFLNDL 

       670        680        690        700        710        720 
LDNDAFFAFI RNIDTIIPFI DDAKTAAFIW KQITAICVEM SLDMDQMSAS LFSTAMNFIR 

       730        740        750        760        770        780 
KKNNTSISGL EIILNCLHFT LRNVNDDVAP TVGSSESHSV FLIKVNNDAA IELPIDQLVD 

       790        800        810        820        830        840 
LFYALNDDDV NLSKLISIFT KICSLPGFEN LTINIIFHPN FYEKIVSFFD TYFNSLLIQI 

       850        860        870        880        890        900 
DLLKFIKLIF SKSLLKLYDY TGQPDPIKQT EPNRRNKATV FKLRAILVQI TEFLNNNWNK 

       910        920        930        940        950        960 
DVPKRNSNQV GGDSVLICQL CEDIRSLSKK GSLQKVSSVT AAIGSSPTKD ERSNLRSSKD 

       970 
KSDGFSVPIT TFQT

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA." Schweitzer B., Philippsen P. Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"CDC15, an essential cell cycle gene in Saccharomyces cerevisiae, encodes a protein kinase domain." Schweitzer B., Philippsen P. Yeast 7:265-273(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: S288c / GRF88.
[3]	Schweitzer B. Submitted (MAY-1993) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 900-902.
[4]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 316; 321 AND 900-902. Strain: ATCC 204508 / S288c.
[7]	"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I." Davies C.J., Hutchison C.A. III Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 865-974.
[8]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[9]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[10]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-562, MASS SPECTROMETRY. Strain: YAL6B.
[11]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-946, MASS SPECTROMETRY.
[12]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309; SER-560; SER-562; THR-564; SER-596 AND SER-946, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X60549 Genomic DNA. Translation: CAA43041.1. X52683 Genomic DNA. Translation: CAA36906.1. L22015 Genomic DNA. Translation: AAC04965.1. M67445 Genomic DNA. Translation: AAA34400.1. BK006935 Genomic DNA. Translation: DAA06996.2.
PIR	S15038.
RefSeq	NP_009411.2. NM_001178218.2.
3D structure databases
ProteinModelPortal	P27636.
SMR	P27636. Positions 12-281.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5728N.
IntAct	P27636. 8 interactions.
MINT	MINT-683916.
STRING	4932.YAR019C.
Proteomic databases
PaxDb	P27636.
PeptideAtlas	P27636.
PRIDE	P27636.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAR019C; YAR019C; YAR019C.
GeneID	851274.
KEGG	sce:YAR019C.
Organism-specific databases
SGD	S000000072. CDC15.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00700000104073.
HOGENOM	HOG000141997.
KO	K06683.
OMA	VKYHGFI.
OrthoDB	EOG4GQTDR.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28875-MONOMER.
BRENDA	2.7.11.1. 984.
Gene expression databases
Genevestigator	P27636.
GermOnline	YAR019C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968257.

Entry information

Entry name

CDC15_YEAST

Accession

Primary (citable) accession number: P27636
Secondary accession number(s): D6VPM6

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 27, 2011
Last modified:	May 29, 2013
This is version 129 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents