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P27616

- PUR7_YEAST

UniProt

P27616 - PUR7_YEAST

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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene
ADE1, YAR015W
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: SGD
  3. protein binding Source: IntAct

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:ADE1
Ordered Locus Names:YAR015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

SGDiS000000070. ADE1.

Pathology & Biotechi

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedUniRule annotation
Chaini2 – 306305Phosphoribosylaminoimidazole-succinocarboxamide synthaseUniRule annotationPRO_0000100929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP27616.
PaxDbiP27616.
PeptideAtlasiP27616.

2D gel databases

SWISS-2DPAGEP27616.

Expressioni

Gene expression databases

GenevestigatoriP27616.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP82P028292EBI-14257,EBI-8659

Protein-protein interaction databases

BioGridi31799. 29 interactions.
IntActiP27616. 2 interactions.
MINTiMINT-659098.
STRINGi4932.YAR015W.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 174
Beta strandi19 – 279
Beta strandi30 – 356
Helixi53 – 6715
Turni68 – 714
Beta strandi72 – 765
Helixi85 – 884
Helixi91 – 944
Helixi96 – 1027
Beta strandi105 – 1106
Beta strandi116 – 1249
Helixi127 – 13610
Beta strandi137 – 1393
Beta strandi153 – 1597
Helixi176 – 1838
Helixi185 – 20925
Beta strandi211 – 22313
Turni224 – 2274
Beta strandi228 – 2336
Turni238 – 2403
Beta strandi241 – 2455
Turni246 – 2483
Turni258 – 2603
Helixi261 – 2699
Helixi282 – 30019

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A48X-ray1.90A2-306[»]
1OBDX-ray1.40A2-306[»]
1OBGX-ray2.05A2-306[»]
2CNQX-ray1.00A2-306[»]
2CNUX-ray1.05A2-306[»]
2CNVX-ray2.00A2-306[»]
ProteinModelPortaliP27616.
SMRiP27616. Positions 2-306.

Miscellaneous databases

EvolutionaryTraceiP27616.

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.

Phylogenomic databases

eggNOGiCOG0152.
HOGENOMiHOG000230360.
KOiK01923.
OMAiFWFDKVK.
OrthoDBiEOG71ZPBX.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27616-1 [UniParc]FASTAAdd to Basket

« Hide

MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI    50
PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT 100
QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE 150
SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS 200
KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK 250
VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT 300
GSKWSH 306
Length:306
Mass (Da):34,603
Last modified:October 5, 2010 - v2
Checksum:iA9AF2D450F25862A
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851E → G in AAA34396. 1 Publication
Sequence conflicti185 – 1851E → G no nucleotide entry 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.
PIRiS20122. JQ1395.
RefSeqiNP_009409.1. NM_001178216.1.

Genome annotation databases

EnsemblFungiiYAR015W; YAR015W; YAR015W.
GeneIDi851272.
KEGGisce:YAR015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1 .
M67445 Genomic DNA. Translation: AAA34398.1 .
L22015 Genomic DNA. Translation: AAC04963.1 .
X60549 Genomic DNA. Translation: CAA43043.1 .
BK006935 Genomic DNA. Translation: DAA06994.1 .
PIRi S20122. JQ1395.
RefSeqi NP_009409.1. NM_001178216.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A48 X-ray 1.90 A 2-306 [» ]
1OBD X-ray 1.40 A 2-306 [» ]
1OBG X-ray 2.05 A 2-306 [» ]
2CNQ X-ray 1.00 A 2-306 [» ]
2CNU X-ray 1.05 A 2-306 [» ]
2CNV X-ray 2.00 A 2-306 [» ]
ProteinModelPortali P27616.
SMRi P27616. Positions 2-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31799. 29 interactions.
IntActi P27616. 2 interactions.
MINTi MINT-659098.
STRINGi 4932.YAR015W.

Chemistry

DrugBanki DB00131. Adenosine monophosphate.

2D gel databases

SWISS-2DPAGE P27616.

Proteomic databases

MaxQBi P27616.
PaxDbi P27616.
PeptideAtlasi P27616.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAR015W ; YAR015W ; YAR015W .
GeneIDi 851272.
KEGGi sce:YAR015W.

Organism-specific databases

SGDi S000000070. ADE1.

Phylogenomic databases

eggNOGi COG0152.
HOGENOMi HOG000230360.
KOi K01923.
OMAi FWFDKVK.
OrthoDBi EOG71ZPBX.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00131 .
BioCyci MetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P27616.
NextBioi 968251.

Gene expression databases

Genevestigatori P27616.

Family and domain databases

Gene3Di 3.30.470.20. 1 hit.
HAMAPi MF_00137. SAICAR_synth.
InterProi IPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view ]
Pfami PF01259. SAICAR_synt. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00081. purC. 1 hit.
PROSITEi PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae."
    Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N.
    Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control."
    Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N.
    Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
    Davies C.J., Hutchison C.A. III
    Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA."
    Schweitzer B., Philippsen P.
    Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis."
    Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S.
    Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), ACETYLATION AT SER-2.

Entry informationi

Entry nameiPUR7_YEAST
AccessioniPrimary (citable) accession number: P27616
Secondary accession number(s): D6VPM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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