Phosphoribosylaminoimidazole-succinocarboxamide synthase - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P27616 (PUR7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 129. History...

Clusters with 100%, 90%, 50% identity |

Documents (5) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	ADE1
Ordered Locus Names:	YAR015W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Subunit structure	Monomer.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from direct assay Ref.2. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay PubMed 14562095. Source: SGD nucleus Inferred from direct assay PubMed 14562095. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from direct assay Ref.2. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSP82	P02829	2	EBI-14257,EBI-8659

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 306

306

Phosphoribosylaminoimidazole-succinocarboxamide synthase

PRO_0000100929

Amino acid modifications

Modified residue

N-acetylmethionine

Modified residue

160

Phosphothreonine Ref.9

Experimental info

Sequence conflict

185

E → G in AAA34396. Ref.1

Sequence conflict

185

E → G no nucleotide entry Ref.2

Secondary structure

306

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P27616 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: A9AF2D450F25862A
Show »

FASTA

306

34,603

        10         20         30         40         50         60 
MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI PEKGILLTKL 

        70         80         90        100        110        120 
SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT QLEDRSLLVH KHKLIPLEVI 

       130        140        150        160        170        180 
VRGYITGSAW KEYVKTGTVH GLKQPQGLKE SQEFPEPIFT PSTKAEQGEH DENISPAQAA 

       190        200        210        220        230        240 
ELVGEDLSRR VAELAVKLYS KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS 

       250        260        270        280        290        300 
SRFWNGASYK VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT 


GSKWSH

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae." Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N. Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control." Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N. Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I." Davies C.J., Hutchison C.A. III Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA." Schweitzer B., Philippsen P. Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-160, MASS SPECTROMETRY.
[10]	"The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis." Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S. Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.

PIR

JQ1395. S20122.

RefSeq

NP_009409.1. NM_001178216.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A48	X-ray	1.90	A	2-306	[»]
1OBD	X-ray	1.40	A	2-306	[»]
1OBG	X-ray	2.05	A	2-306	[»]
2CNQ	X-ray	1.00	A	2-306	[»]
2CNU	X-ray	1.05	A	2-306	[»]
2CNV	X-ray	2.00	A	2-306	[»]

ProteinModelPortal

P27616.

SMR

P27616. Positions 2-306.

ModBase

Search...

Protein-protein interaction databases

IntAct

P27616. 2 interactions.

MINT

MINT-659098.

STRING

4932.YAR015W.

2D gel databases

SWISS-2DPAGE

P27616.

Proteomic databases

PaxDb

P27616.

PeptideAtlas

P27616.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YAR015W; YAR015W; YAR015W.

GeneID

851272.

KEGG

sce:YAR015W.

Organism-specific databases

SGD

S000000070. ADE1.

Phylogenomic databases

eggNOG

COG0152.

HOGENOM

HOG000230360.

K01923.

OMA

DSSRYWL.

OrthoDB

EOG4JMBZN.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-488.

UniPathway

UPA00074; UER00131.

Gene expression databases

Genevestigator

P27616.

GermOnline

YAR015W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

3.30.470.20. 1 hit.

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

PANTHER

PTHR11609. PTHR11609. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. purC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00131. Adenosine monophosphate.

EvolutionaryTrace

P27616.

NextBio

968251.

Entry information

Entry name

PUR7_YEAST

Accession

Primary (citable) accession number: P27616
Secondary accession number(s): D6VPM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 5, 2010
Last modified:	April 3, 2013
This is version 129 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

2D gel databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents