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P27616

- PUR7_YEAST

UniProt

P27616 - PUR7_YEAST

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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

ADE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: SGD

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:ADE1
Ordered Locus Names:YAR015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

SGDiS000000070. ADE1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 306305Phosphoribosylaminoimidazole-succinocarboxamide synthasePRO_0000100929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP27616.
PaxDbiP27616.
PeptideAtlasiP27616.

2D gel databases

SWISS-2DPAGEP27616.

Expressioni

Gene expression databases

GenevestigatoriP27616.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP82P028292EBI-14257,EBI-8659

Protein-protein interaction databases

BioGridi31799. 30 interactions.
IntActiP27616. 2 interactions.
MINTiMINT-659098.
STRINGi4932.YAR015W.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 174Combined sources
Beta strandi19 – 279Combined sources
Beta strandi30 – 356Combined sources
Helixi53 – 6715Combined sources
Turni68 – 714Combined sources
Beta strandi72 – 765Combined sources
Helixi85 – 884Combined sources
Helixi91 – 944Combined sources
Helixi96 – 1027Combined sources
Beta strandi105 – 1106Combined sources
Beta strandi116 – 1249Combined sources
Helixi127 – 13610Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi153 – 1597Combined sources
Helixi176 – 1838Combined sources
Helixi185 – 20925Combined sources
Beta strandi211 – 22313Combined sources
Turni224 – 2274Combined sources
Beta strandi228 – 2336Combined sources
Turni238 – 2403Combined sources
Beta strandi241 – 2455Combined sources
Turni246 – 2483Combined sources
Turni258 – 2603Combined sources
Helixi261 – 2699Combined sources
Helixi282 – 30019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A48X-ray1.90A2-306[»]
1OBDX-ray1.40A2-306[»]
1OBGX-ray2.05A2-306[»]
2CNQX-ray1.00A2-306[»]
2CNUX-ray1.05A2-306[»]
2CNVX-ray2.00A2-306[»]
ProteinModelPortaliP27616.
SMRiP27616. Positions 2-306.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27616.

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0152.
HOGENOMiHOG000230360.
InParanoidiP27616.
KOiK01923.
OMAiFWFDKVK.
OrthoDBiEOG71ZPBX.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27616-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI
60 70 80 90 100
PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT
110 120 130 140 150
QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE
160 170 180 190 200
SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS
210 220 230 240 250
KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK
260 270 280 290 300
VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT

GSKWSH
Length:306
Mass (Da):34,603
Last modified:October 5, 2010 - v2
Checksum:iA9AF2D450F25862A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851E → G in AAA34396. (PubMed:3013210)Curated
Sequence conflicti185 – 1851E → G no nucleotide entry (PubMed:1756975)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.
PIRiS20122. JQ1395.
RefSeqiNP_009409.1. NM_001178216.1.

Genome annotation databases

EnsemblFungiiYAR015W; YAR015W; YAR015W.
GeneIDi851272.
KEGGisce:YAR015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1 .
M67445 Genomic DNA. Translation: AAA34398.1 .
L22015 Genomic DNA. Translation: AAC04963.1 .
X60549 Genomic DNA. Translation: CAA43043.1 .
BK006935 Genomic DNA. Translation: DAA06994.1 .
PIRi S20122. JQ1395.
RefSeqi NP_009409.1. NM_001178216.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1A48 X-ray 1.90 A 2-306 [» ]
1OBD X-ray 1.40 A 2-306 [» ]
1OBG X-ray 2.05 A 2-306 [» ]
2CNQ X-ray 1.00 A 2-306 [» ]
2CNU X-ray 1.05 A 2-306 [» ]
2CNV X-ray 2.00 A 2-306 [» ]
ProteinModelPortali P27616.
SMRi P27616. Positions 2-306.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31799. 30 interactions.
IntActi P27616. 2 interactions.
MINTi MINT-659098.
STRINGi 4932.YAR015W.

2D gel databases

SWISS-2DPAGE P27616.

Proteomic databases

MaxQBi P27616.
PaxDbi P27616.
PeptideAtlasi P27616.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAR015W ; YAR015W ; YAR015W .
GeneIDi 851272.
KEGGi sce:YAR015W.

Organism-specific databases

SGDi S000000070. ADE1.

Phylogenomic databases

eggNOGi COG0152.
HOGENOMi HOG000230360.
InParanoidi P27616.
KOi K01923.
OMAi FWFDKVK.
OrthoDBi EOG71ZPBX.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00131 .
BioCyci MetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.

Miscellaneous databases

EvolutionaryTracei P27616.
NextBioi 968251.

Gene expression databases

Genevestigatori P27616.

Family and domain databases

Gene3Di 3.30.470.20. 1 hit.
HAMAPi MF_00137. SAICAR_synth.
InterProi IPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view ]
Pfami PF01259. SAICAR_synt. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00081. purC. 1 hit.
PROSITEi PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae."
    Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N.
    Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control."
    Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N.
    Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
    Davies C.J., Hutchison C.A. III
    Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA."
    Schweitzer B., Philippsen P.
    Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis."
    Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S.
    Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), ACETYLATION AT SER-2.

Entry informationi

Entry nameiPUR7_YEAST
AccessioniPrimary (citable) accession number: P27616
Secondary accession number(s): D6VPM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: November 26, 2014
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3