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P27616 (PUR7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase

EC=6.3.2.6
Alternative name(s):
SAICAR synthetase
Gene names
Name:ADE1
Ordered Locus Names:YAR015W
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length306 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP-Rule MF_00137

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2. HAMAP-Rule MF_00137

Subunit structure

Monomer.

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SAICAR synthetase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

HSP82P028292EBI-14257,EBI-8659

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed HAMAP-Rule MF_00137
Chain2 – 306305Phosphoribosylaminoimidazole-succinocarboxamide synthase HAMAP-Rule MF_00137
PRO_0000100929

Amino acid modifications

Modified residue21N-acetylserine Ref.9 Ref.10

Experimental info

Sequence conflict1851E → G in AAA34396. Ref.1
Sequence conflict1851E → G no nucleotide entry Ref.2

Secondary structure

........................................... 306
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P27616 [UniParc].

Last modified October 5, 2010. Version 2.
Checksum: A9AF2D450F25862A

FASTA30634,603
        10         20         30         40         50         60 
MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI PEKGILLTKL 

        70         80         90        100        110        120 
SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT QLEDRSLLVH KHKLIPLEVI 

       130        140        150        160        170        180 
VRGYITGSAW KEYVKTGTVH GLKQPQGLKE SQEFPEPIFT PSTKAEQGEH DENISPAQAA 

       190        200        210        220        230        240 
ELVGEDLSRR VAELAVKLYS KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS 

       250        260        270        280        290        300 
SRFWNGASYK VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT 


GSKWSH 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae."
Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N.
Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control."
Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N.
Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
Davies C.J., Hutchison C.A. III
Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[5]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[6]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[7]"NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA."
Schweitzer B., Philippsen P.
Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
[8]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB."
Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.
Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis."
Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S.
Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), ACETYLATION AT SER-2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.
PIRJQ1395. S20122.
RefSeqNP_009409.1. NM_001178216.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1A48X-ray1.90A2-306[»]
1OBDX-ray1.40A2-306[»]
1OBGX-ray2.05A2-306[»]
2CNQX-ray1.00A2-306[»]
2CNUX-ray1.05A2-306[»]
2CNVX-ray2.00A2-306[»]
ProteinModelPortalP27616.
SMRP27616. Positions 2-306.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31799. 29 interactions.
IntActP27616. 2 interactions.
MINTMINT-659098.
STRING4932.YAR015W.

Chemistry

DrugBankDB00131. Adenosine monophosphate.

2D gel databases

SWISS-2DPAGEP27616.

Proteomic databases

MaxQBP27616.
PaxDbP27616.
PeptideAtlasP27616.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAR015W; YAR015W; YAR015W.
GeneID851272.
KEGGsce:YAR015W.

Organism-specific databases

SGDS000000070. ADE1.

Phylogenomic databases

eggNOGCOG0152.
HOGENOMHOG000230360.
KOK01923.
OMAFWFDKVK.
OrthoDBEOG71ZPBX.

Enzyme and pathway databases

BioCycMetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.
UniPathwayUPA00074; UER00131.

Gene expression databases

GenevestigatorP27616.

Family and domain databases

Gene3D3.30.470.20. 1 hit.
HAMAPMF_00137. SAICAR_synth.
InterProIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsTIGR00081. purC. 1 hit.
PROSITEPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP27616.
NextBio968251.

Entry information

Entry namePUR7_YEAST
AccessionPrimary (citable) accession number: P27616
Secondary accession number(s): D6VPM4
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways