P27616 (PUR7_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase
EC=6.3.2.6

Alternative name(s):
SAICAR synthetase

Gene names

Name:	ADE1
Ordered Locus Names:	YAR015W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	306 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate: step 1/2.
Subunit structure	Monomer.
Miscellaneous	Present with 4280 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from direct assay Ref.2. Source: SGD
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from direct assay Ref.2. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
HSP82	P02829	2	EBI-14257,EBI-8659

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 306

306

Phosphoribosylaminoimidazole-succinocarboxamide synthase

PRO_0000100929

Amino acid modifications

Modified residue

1

1

N-acetylmethionine

Modified residue

160

1

Phosphothreonine Ref.9

Experimental info

Sequence conflict

185

1

E → G in AAA34396. Ref.1

Sequence conflict

185

1

E → G no nucleotide entry Ref.2

Secondary structure

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306

Helix Strand Turn

Details...

Turn

9 – 10

2

Beta strand

14 – 17

4

Beta strand

19 – 27

9

Turn

28 – 29

2

Beta strand

30 – 35

6

Turn

42 – 43

2

Turn

51 – 52

2

Helix

53 – 67

15

Turn

68 – 71

4

Beta strand

73 – 76

4

Turn

81 – 82

2

Helix

85 – 88

4

Helix

91 – 94

4

Helix

96 – 102

7

Turn

103 – 104

2

Beta strand

105 – 110

6

Beta strand

116 – 124

9

Helix

127 – 136

10

Beta strand

137 – 139

3

Turn

140 – 141

2

Turn

146 – 147

2

Turn

150 – 151

2

Beta strand

153 – 159

7

Helix

176 – 183

8

Helix

185 – 208

24

Turn

209 – 210

2

Beta strand

211 – 223

13

Turn

224 – 227

4

Beta strand

228 – 233

6

Turn

238 – 240

3

Beta strand

241 – 246

6

Turn

247 – 248

2

Turn

251 – 252

2

Turn

258 – 260

3

Helix

261 – 269

9

Turn

270 – 271

2

Turn

273 – 274

2

Helix

282 – 300

19

Sequences

	Sequence		Length	Mass (Da)
	P27616 [UniParc]. Last modified October 5, 2010. Version 2. Checksum: A9AF2D450F25862A	FASTA	306	34,603
	10 20 30 40 50 60 MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI PEKGILLTKL 70 80 90 100 110 120 SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT QLEDRSLLVH KHKLIPLEVI 130 140 150 160 170 180 VRGYITGSAW KEYVKTGTVH GLKQPQGLKE SQEFPEPIFT PSTKAEQGEH DENISPAQAA 190 200 210 220 230 240 ELVGEDLSRR VAELAVKLYS KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS 250 260 270 280 290 300 SRFWNGASYK VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT GSKWSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae." Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N. Bioorg. Khim. 12:555-558(1986) [PubMed: 3013210] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control." Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N. Gene 109:143-147(1991) [PubMed: 1756975] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I." Davies C.J., Hutchison C.A. III Nucleic Acids Res. 19:5731-5738(1991) [PubMed: 1658741] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region." Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H. Yeast 10:535-541(1994) [PubMed: 7941740] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[7]	"NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA." Schweitzer B., Philippsen P. Mol. Gen. Genet. 234:164-167(1992) [PubMed: 1495480] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-160, MASS SPECTROMETRY.
[10]	"The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis." Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S. Structure 6:363-376(1998) [PubMed: 9551557] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.

PIR

JQ1395. S20122.

RefSeq

NP_009409.1. NM_001178216.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1A48	X-ray	1.90	A	2-306	[»]
1OBD	X-ray	1.40	A	2-306	[»]
1OBG	X-ray	2.05	A	2-306	[»]
2CNQ	X-ray	1.00	A	2-306	[»]
2CNU	X-ray	1.05	A	2-306	[»]
2CNV	X-ray	2.00	A	2-306	[»]

ProteinModelPortal

P27616.

SMR

P27616. Positions 2-306.

ModBase

Search...

Protein-protein interaction databases

IntAct

P27616. 3 interactions.

MINT

MINT-659098.

STRING

P27616.

2D gel databases

SWISS-2DPAGE

P27616.

Proteomic databases

PeptideAtlas

P27616.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YAR015W; YAR015W; YAR015W.

GeneID

851272.

KEGG

sce:YAR015W.

Organism-specific databases

SGD

S000000070. ADE1.

Phylogenomic databases

eggNOG

fuNOG04520.

GeneTree

EFGT00050000005120.

HOGENOM

HBG555040.

OMA

LTLMDEV.

OrthoDB

EOG4JMBZN.

Enzyme and pathway databases

BioCyc

MetaCyc:MONOMER-488.

Gene expression databases

ArrayExpress

P27616.

Genevestigator

P27616.

GermOnline

YAR015W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR013816. ATP_grasp_subdomain_2.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]

Gene3D

G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.

KO

K01923.

PANTHER

PTHR11609. SAICAR_synt. 1 hit.

Pfam

PF01259. SAICAR_synt. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00081. PurC. 1 hit.

PROSITE

PS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

DrugBank

DB00131. Adenosine monophosphate.

Entry information

Entry name

PUR7_YEAST

Accession

Primary (citable) accession number: P27616
Secondary accession number(s): D6VPM4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	October 5, 2010
Last modified:	December 14, 2011
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program