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Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

ADE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: SGD

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: SGD
  2. purine nucleotide biosynthetic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.
BRENDAi6.3.2.6. 984.
UniPathwayiUPA00074; UER00131.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Gene namesi
Name:ADE1
Ordered Locus Names:YAR015W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAR015W.
SGDiS000000070. ADE1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed2 Publications
Chaini2 – 306305Phosphoribosylaminoimidazole-succinocarboxamide synthasePRO_0000100929Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine2 Publications

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP27616.
PaxDbiP27616.
PeptideAtlasiP27616.

2D gel databases

SWISS-2DPAGEP27616.

Expressioni

Gene expression databases

GenevestigatoriP27616.

Interactioni

Subunit structurei

Monomer.

Binary interactionsi

WithEntry#Exp.IntActNotes
HSP82P028292EBI-14257,EBI-8659

Protein-protein interaction databases

BioGridi31799. 30 interactions.
IntActiP27616. 2 interactions.
MINTiMINT-659098.
STRINGi4932.YAR015W.

Structurei

Secondary structure

1
306
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi14 – 174Combined sources
Beta strandi19 – 279Combined sources
Beta strandi30 – 356Combined sources
Helixi53 – 6715Combined sources
Turni68 – 714Combined sources
Beta strandi72 – 765Combined sources
Helixi85 – 884Combined sources
Helixi91 – 944Combined sources
Helixi96 – 1027Combined sources
Beta strandi105 – 1106Combined sources
Beta strandi116 – 1249Combined sources
Helixi127 – 13610Combined sources
Beta strandi137 – 1393Combined sources
Beta strandi153 – 1597Combined sources
Helixi176 – 1838Combined sources
Helixi185 – 20925Combined sources
Beta strandi211 – 22313Combined sources
Turni224 – 2274Combined sources
Beta strandi228 – 2336Combined sources
Turni238 – 2403Combined sources
Beta strandi241 – 2455Combined sources
Turni246 – 2483Combined sources
Turni258 – 2603Combined sources
Helixi261 – 2699Combined sources
Helixi282 – 30019Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A48X-ray1.90A2-306[»]
1OBDX-ray1.40A2-306[»]
1OBGX-ray2.05A2-306[»]
2CNQX-ray1.00A2-306[»]
2CNUX-ray1.05A2-306[»]
2CNVX-ray2.00A2-306[»]
ProteinModelPortaliP27616.
SMRiP27616. Positions 2-306.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27616.

Family & Domainsi

Sequence similaritiesi

Belongs to the SAICAR synthetase family.Curated

Phylogenomic databases

eggNOGiCOG0152.
HOGENOMiHOG000230360.
InParanoidiP27616.
KOiK01923.
OMAiFWPADSY.
OrthoDBiEOG71ZPBX.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27616-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI
60 70 80 90 100
PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT
110 120 130 140 150
QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE
160 170 180 190 200
SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS
210 220 230 240 250
KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK
260 270 280 290 300
VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT

GSKWSH
Length:306
Mass (Da):34,603
Last modified:October 5, 2010 - v2
Checksum:iA9AF2D450F25862A
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti185 – 1851E → G in AAA34396 (PubMed:3013210).Curated
Sequence conflicti185 – 1851E → G no nucleotide entry (PubMed:1756975).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.
PIRiS20122. JQ1395.
RefSeqiNP_009409.1. NM_001178216.1.

Genome annotation databases

EnsemblFungiiYAR015W; YAR015W; YAR015W.
GeneIDi851272.
KEGGisce:YAR015W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M61209 Genomic DNA. Translation: AAA34396.1.
M67445 Genomic DNA. Translation: AAA34398.1.
L22015 Genomic DNA. Translation: AAC04963.1.
X60549 Genomic DNA. Translation: CAA43043.1.
BK006935 Genomic DNA. Translation: DAA06994.1.
PIRiS20122. JQ1395.
RefSeqiNP_009409.1. NM_001178216.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A48X-ray1.90A2-306[»]
1OBDX-ray1.40A2-306[»]
1OBGX-ray2.05A2-306[»]
2CNQX-ray1.00A2-306[»]
2CNUX-ray1.05A2-306[»]
2CNVX-ray2.00A2-306[»]
ProteinModelPortaliP27616.
SMRiP27616. Positions 2-306.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31799. 30 interactions.
IntActiP27616. 2 interactions.
MINTiMINT-659098.
STRINGi4932.YAR015W.

2D gel databases

SWISS-2DPAGEP27616.

Proteomic databases

MaxQBiP27616.
PaxDbiP27616.
PeptideAtlasiP27616.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAR015W; YAR015W; YAR015W.
GeneIDi851272.
KEGGisce:YAR015W.

Organism-specific databases

EuPathDBiFungiDB:YAR015W.
SGDiS000000070. ADE1.

Phylogenomic databases

eggNOGiCOG0152.
HOGENOMiHOG000230360.
InParanoidiP27616.
KOiK01923.
OMAiFWPADSY.
OrthoDBiEOG71ZPBX.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00131.
BioCyciMetaCyc:MONOMER-488.
YEAST:YAR015W-MONOMER.
BRENDAi6.3.2.6. 984.

Miscellaneous databases

EvolutionaryTraceiP27616.
NextBioi968251.
PROiP27616.

Gene expression databases

GenevestigatoriP27616.

Family and domain databases

Gene3Di3.30.470.20. 1 hit.
HAMAPiMF_00137. SAICAR_synth.
InterProiIPR013816. ATP_grasp_subdomain_2.
IPR028923. SAICAR_synt/ADE2_N.
IPR001636. SAICAR_synth.
IPR018236. SAICAR_synthetase_CS.
[Graphical view]
PfamiPF01259. SAICAR_synt. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00081. purC. 1 hit.
PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
PS01058. SAICAR_SYNTHETASE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae."
    Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N.
    Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control."
    Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N.
    Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
    Davies C.J., Hutchison C.A. III
    Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
    Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
    Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  7. "NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA."
    Schweitzer B., Philippsen P.
    Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
  8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].
  10. "The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis."
    Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S.
    Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), ACETYLATION AT SER-2.

Entry informationi

Entry nameiPUR7_YEAST
AccessioniPrimary (citable) accession number: P27616
Secondary accession number(s): D6VPM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: October 5, 2010
Last modified: April 29, 2015
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.