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P27616

- PUR7_YEAST

UniProt

P27616 - PUR7_YEAST

Protein

Phosphoribosylaminoimidazole-succinocarboxamide synthase

Gene

ADE1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (05 Oct 2010)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: SGD
    3. protein binding Source: IntAct

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: SGD
    2. purine nucleotide biosynthetic process Source: SGD

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-488.
    YEAST:YAR015W-MONOMER.
    UniPathwayiUPA00074; UER00131.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
    Alternative name(s):
    SAICAR synthetase
    Gene namesi
    Name:ADE1
    Ordered Locus Names:YAR015W
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    SGDiS000000070. ADE1.

    Pathology & Biotechi

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed2 Publications
    Chaini2 – 306305Phosphoribosylaminoimidazole-succinocarboxamide synthasePRO_0000100929Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserine2 Publications

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP27616.
    PaxDbiP27616.
    PeptideAtlasiP27616.

    2D gel databases

    SWISS-2DPAGEP27616.

    Expressioni

    Gene expression databases

    GenevestigatoriP27616.

    Interactioni

    Subunit structurei

    Monomer.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    HSP82P028292EBI-14257,EBI-8659

    Protein-protein interaction databases

    BioGridi31799. 29 interactions.
    IntActiP27616. 2 interactions.
    MINTiMINT-659098.
    STRINGi4932.YAR015W.

    Structurei

    Secondary structure

    1
    306
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi14 – 174
    Beta strandi19 – 279
    Beta strandi30 – 356
    Helixi53 – 6715
    Turni68 – 714
    Beta strandi72 – 765
    Helixi85 – 884
    Helixi91 – 944
    Helixi96 – 1027
    Beta strandi105 – 1106
    Beta strandi116 – 1249
    Helixi127 – 13610
    Beta strandi137 – 1393
    Beta strandi153 – 1597
    Helixi176 – 1838
    Helixi185 – 20925
    Beta strandi211 – 22313
    Turni224 – 2274
    Beta strandi228 – 2336
    Turni238 – 2403
    Beta strandi241 – 2455
    Turni246 – 2483
    Turni258 – 2603
    Helixi261 – 2699
    Helixi282 – 30019

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1A48X-ray1.90A2-306[»]
    1OBDX-ray1.40A2-306[»]
    1OBGX-ray2.05A2-306[»]
    2CNQX-ray1.00A2-306[»]
    2CNUX-ray1.05A2-306[»]
    2CNVX-ray2.00A2-306[»]
    ProteinModelPortaliP27616.
    SMRiP27616. Positions 2-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27616.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the SAICAR synthetase family.Curated

    Phylogenomic databases

    eggNOGiCOG0152.
    HOGENOMiHOG000230360.
    KOiK01923.
    OMAiFWFDKVK.
    OrthoDBiEOG71ZPBX.

    Family and domain databases

    Gene3Di3.30.470.20. 1 hit.
    HAMAPiMF_00137. SAICAR_synth.
    InterProiIPR013816. ATP_grasp_subdomain_2.
    IPR028923. SAICAR_synt/ADE2_N.
    IPR001636. SAICAR_synth.
    IPR018236. SAICAR_synthetase_CS.
    [Graphical view]
    PfamiPF01259. SAICAR_synt. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00081. purC. 1 hit.
    PROSITEiPS01057. SAICAR_SYNTHETASE_1. 1 hit.
    PS01058. SAICAR_SYNTHETASE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27616-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSITKTELDG ILPLVARGKV RDIYEVDAGT LLFVATDRIS AYDVIMENSI    50
    PEKGILLTKL SEFWFKFLSN DVRNHLVDIA PGKTIFDYLP AKLSEPKYKT 100
    QLEDRSLLVH KHKLIPLEVI VRGYITGSAW KEYVKTGTVH GLKQPQGLKE 150
    SQEFPEPIFT PSTKAEQGEH DENISPAQAA ELVGEDLSRR VAELAVKLYS 200
    KCKDYAKEKG IIIADTKFEF GIDEKTNEII LVDEVLTPDS SRFWNGASYK 250
    VGESQDSYDK QFLRDWLTAN KLNGVNGVKM PQDIVDRTRA KYIEAYETLT 300
    GSKWSH 306
    Length:306
    Mass (Da):34,603
    Last modified:October 5, 2010 - v2
    Checksum:iA9AF2D450F25862A
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti185 – 1851E → G in AAA34396. (PubMed:3013210)Curated
    Sequence conflicti185 – 1851E → G no nucleotide entry (PubMed:1756975)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61209 Genomic DNA. Translation: AAA34396.1.
    M67445 Genomic DNA. Translation: AAA34398.1.
    L22015 Genomic DNA. Translation: AAC04963.1.
    X60549 Genomic DNA. Translation: CAA43043.1.
    BK006935 Genomic DNA. Translation: DAA06994.1.
    PIRiS20122. JQ1395.
    RefSeqiNP_009409.1. NM_001178216.1.

    Genome annotation databases

    EnsemblFungiiYAR015W; YAR015W; YAR015W.
    GeneIDi851272.
    KEGGisce:YAR015W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M61209 Genomic DNA. Translation: AAA34396.1 .
    M67445 Genomic DNA. Translation: AAA34398.1 .
    L22015 Genomic DNA. Translation: AAC04963.1 .
    X60549 Genomic DNA. Translation: CAA43043.1 .
    BK006935 Genomic DNA. Translation: DAA06994.1 .
    PIRi S20122. JQ1395.
    RefSeqi NP_009409.1. NM_001178216.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1A48 X-ray 1.90 A 2-306 [» ]
    1OBD X-ray 1.40 A 2-306 [» ]
    1OBG X-ray 2.05 A 2-306 [» ]
    2CNQ X-ray 1.00 A 2-306 [» ]
    2CNU X-ray 1.05 A 2-306 [» ]
    2CNV X-ray 2.00 A 2-306 [» ]
    ProteinModelPortali P27616.
    SMRi P27616. Positions 2-306.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31799. 29 interactions.
    IntActi P27616. 2 interactions.
    MINTi MINT-659098.
    STRINGi 4932.YAR015W.

    Chemistry

    DrugBanki DB00131. Adenosine monophosphate.

    2D gel databases

    SWISS-2DPAGE P27616.

    Proteomic databases

    MaxQBi P27616.
    PaxDbi P27616.
    PeptideAtlasi P27616.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAR015W ; YAR015W ; YAR015W .
    GeneIDi 851272.
    KEGGi sce:YAR015W.

    Organism-specific databases

    SGDi S000000070. ADE1.

    Phylogenomic databases

    eggNOGi COG0152.
    HOGENOMi HOG000230360.
    KOi K01923.
    OMAi FWFDKVK.
    OrthoDBi EOG71ZPBX.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00131 .
    BioCyci MetaCyc:MONOMER-488.
    YEAST:YAR015W-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P27616.
    NextBioi 968251.

    Gene expression databases

    Genevestigatori P27616.

    Family and domain databases

    Gene3Di 3.30.470.20. 1 hit.
    HAMAPi MF_00137. SAICAR_synth.
    InterProi IPR013816. ATP_grasp_subdomain_2.
    IPR028923. SAICAR_synt/ADE2_N.
    IPR001636. SAICAR_synth.
    IPR018236. SAICAR_synthetase_CS.
    [Graphical view ]
    Pfami PF01259. SAICAR_synt. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00081. purC. 1 hit.
    PROSITEi PS01057. SAICAR_SYNTHETASE_1. 1 hit.
    PS01058. SAICAR_SYNTHETASE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of the ADE1 gene of the yeast Saccharomyces cerevisiae."
      Myasnikov A.N., Plavnik Y.A., Sasnauskas K.V., Gedminene G.K., Yanulaitis A.A., Smirnov M.N.
      Bioorg. Khim. 12:555-558(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Saccharomyces cerevisiae ADE1 gene: structure, overexpression and possible regulation by general amino acid control."
      Myasnikov A.N., Sasnauskas K.V., Janulaitis A.A., Smirnov M.N.
      Gene 109:143-147(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "A directed DNA sequencing strategy based upon Tn3 transposon mutagenesis: application to the ADE1 locus on Saccharomyces cerevisiae chromosome I."
      Davies C.J., Hutchison C.A. III
      Nucleic Acids Res. 19:5731-5738(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "Sequencing of chromosome I of Saccharomyces cerevisiae: analysis of the 42 kbp SPO7-CENI-CDC15 region."
      Clark M.W., Keng T., Storms R.K., Zhong W.-W., Fortin N., Zeng B., Delaney S., Ouellette B.F.F., Barton A.B., Kaback D.B., Bussey H.
      Yeast 10:535-541(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "NPK1, a nonessential protein kinase gene in Saccharomyces cerevisiae with similarity to Aspergillus nidulans nimA."
      Schweitzer B., Philippsen P.
      Mol. Gen. Genet. 234:164-167(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 285-306.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "The structure of SAICAR synthase: an enzyme in the de novo pathway of purine nucleotide biosynthesis."
      Levdikov V.M., Barynin V.V., Grebenko A.I., Melik-Adamyan W.R., Lamzin V.S., Wilson K.S.
      Structure 6:363-376(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS), ACETYLATION AT SER-2.

    Entry informationi

    Entry nameiPUR7_YEAST
    AccessioniPrimary (citable) accession number: P27616
    Secondary accession number(s): D6VPM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: October 5, 2010
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 4280 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3