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P27614 (CBPS_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carboxypeptidase S
EC=3.4.17.4
Alternative name(s):
GLY-X carboxypeptidase
YSCS
Gene names
Name:CPS1
Synonyms:CPS
Ordered Locus Names:YJL172W
ORF Names:J0510
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length576 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Necessary for use of certain peptides as sole nitrogen source. May also cleave intracellularly generated peptides to recycle amino acids for protein synthesis.

Catalytic activity

Release of a C-terminal amino acid from a peptide in which glycine is the penultimate amino acid, e.g. Z-Gly-|-Leu.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subunit structure

yscS is synthesized as one polypeptide chain precursor which after carbohydrate modification in the secretory pathway yields two active precursor molecules. The proteolytically unprocessed forms are associated with the membrane, whereas the mature forms of the enzyme are soluble.

Subcellular location

Vacuole membrane; Single-pass membrane protein. Note: Lysosome-like vacuoles. Ref.5 Ref.7

Domain

The transmembrane domain contains polar residues that mediate the recognition by TUL1.

Post-translational modification

Glycosylated.

Ubiquitinated. Ubiquitination mediates sorting into internal vesicles in late endosomes. TUL1 is required for ubiquitination. Ref.8

Miscellaneous

Present with 721 molecules/cell in log phase SD medium. Ref.9

Sequence similarities

Belongs to the peptidase M20A family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 576576Carboxypeptidase S
PRO_0000185271

Regions

Topological domain1 – 1919Cytoplasmic Potential
Transmembrane20 – 4021Helical; Potential
Topological domain41 – 576536Lumenal Potential

Sites

Active site1701 By similarity
Active site2391Proton acceptor By similarity
Metal binding1681Zinc 2 By similarity
Metal binding2051Zinc 1 By similarity
Metal binding2051Zinc 2 By similarity
Metal binding2401Zinc 1 By similarity
Metal binding2681Zinc 2 By similarity
Metal binding5471Zinc 1 By similarity

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential
Glycosylation2281N-linked (GlcNAc...) Potential
Glycosylation3811N-linked (GlcNAc...) Potential
Glycosylation5251N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict3871S → T in CAA40571. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P27614 [UniParc].

Last modified November 1, 1995. Version 2.
Checksum: 5CBB536D421B5F70

FASTA57664,597
        10         20         30         40         50         60 
MIALPVEKAP RKSLWQRHRA FISGIVALII IGTFFLTSGL HPAPPHEAKR PHHGKGPMHS 

        70         80         90        100        110        120 
PKCEKIEPLS PSFKHSVDTI LHDPAFRNSS IEKLSNAVRI PTVVQDKNPN PADDPDFYKH 

       130        140        150        160        170        180 
FYELHDYFEK TFPNIHKHLK LEKVNELGLL YTWEGSDPDL KPLLLMAHQD VVPVNNETLS 

       190        200        210        220        230        240 
SWKFPPFSGH YDPETDFVWG RGSNDCKNLL IAEFEAIEQL LIDGFKPNRT IVMSLGFDEE 

       250        260        270        280        290        300 
ASGTLGAASL ASFLHERYGD DGIYSIIDEG EGIMEVDKDV FVATPINAEK GYVDFEVSIL 

       310        320        330        340        350        360 
GHGGHSSVPP DHTTIGIASE LITEFEANPF DYEFEFDNPI YGLLTCAAEH SKSLSKDVKK 

       370        380        390        400        410        420 
TILGAPFCPR RKDKLVEYIS NQSHLRSLIR TTQAVDIING GVKANALPET TRFLINHRIN 

       430        440        450        460        470        480 
LHSSVAEVFE RNIEYAKKIA EKYGYGLSKN GDDYIIPETE LGHIDITLLR ELEPAPLSPS 

       490        500        510        520        530        540 
SGPVWDILAG TIQDVFENGV LQNNEEFYVT TGLFSGNTDT KYYWNLSKNI YRFVGSIIDI 

       550        560        570 
DLLKTLHSVN EHVDVPGHLS AIAFVYEYIV NVNEYA

« Hide

References

« Hide 'large scale' references
[1]"Carboxypeptidase yscS: gene structure and function of the vacuolar enzyme."
Spormann D.O., Heim J., Wolf D.H.
Eur. J. Biochem. 197:399-405(1991) [PubMed: 2026161] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Molecular cloning and sequencing of genomic DNA encoding yeast vacuolar carboxypeptidase yscS."
Bordallo J., Bordallo C., Gascon S., Suarez-Rendueles P.
FEBS Lett. 283:27-32(1991) [PubMed: 1709881] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204510 / AB320.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"Biogenesis of the yeast vacuole (lysosome). The precursor forms of the soluble hydrolase carboxypeptidase yscS are associated with the vacuolar membrane."
Spormann D.O., Heim J., Wolf D.H.
J. Biol. Chem. 267:8021-8029(1992) [PubMed: 1569061] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[6]"Protein family classification based on searching a database of blocks."
Henikoff S., Henikoff J.G.
Genomics 19:97-107(1994) [PubMed: 8188249] [Abstract]
Cited for: SIMILARITY TO ARGE/DAPE/ACY1/CPG2/YSCS FAMILY.
[7]"Sorting of proteins into multivesicular bodies: ubiquitin-dependent and -independent targeting."
Reggiori F., Pelham H.R.B.
EMBO J. 20:5176-5186(2001) [PubMed: 11566881] [Abstract]
Cited for: SUBCELLULAR LOCATION.
[8]"A transmembrane ubiquitin ligase required to sort membrane proteins into multivesicular bodies."
Reggiori F., Pelham H.R.B.
Nat. Cell Biol. 4:117-123(2002) [PubMed: 11788821] [Abstract]
Cited for: UBIQUITINATION BY TUL1.
[9]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X57316 Genomic DNA. Translation: CAA40571.1.
X63068 Genomic DNA. Translation: CAA44790.1.
Z49447 Genomic DNA. Translation: CAA89467.1.
BK006943 Genomic DNA. Translation: DAA08632.1.
PIRS16693.
RefSeqNP_012363.1. NM_001181605.1.

3D structure databases

ProteinModelPortalP27614.
SMRP27614. Positions 87-297.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-5566N.
IntActP27614. 2 interactions.
MINTMINT-561556.
STRINGP27614.

Protein family/group databases

MEROPSM20.002.

Proteomic databases

PeptideAtlasP27614.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJL172W; YJL172W; YJL172W.
GeneID853267.
KEGGsce:YJL172W.
NMPDRfig|4932.3.peg.3327.

Organism-specific databases

CYGDYJL172w.
SGDS000003708. CPS1.

Phylogenomic databases

eggNOGfuNOG05817.
GeneTreeEFGT00050000005823.
HOGENOMHBG324099.
OMALAHQDVV.
OrthoDBEOG4936SG.

Gene expression databases

ArrayExpressP27614.
GenevestigatorP27614.
GermOnlineYJL172W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001261. ArgE/DapE_CS.
IPR017141. Pept_M20_carboxypep.
IPR002933. Peptidase_M20.
IPR011650. Peptidase_M20_dimer.
[Graphical view]
KOK01293.
PfamPF07687. M20_dimer. 1 hit.
PF01546. Peptidase_M20. 1 hit.
[Graphical view]
PIRSFPIRSF037217. Carboxypeptidase_S. 1 hit.
SUPFAMSSF55031. Peptidase_M20_dimer. 1 hit.
PROSITEPS00758. ARGE_DAPE_CPG2_1. 1 hit.
PS00759. ARGE_DAPE_CPG2_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio973535.

Entry information

Entry nameCBPS_YEAST
AccessionPrimary (citable) accession number: P27614
Secondary accession number(s): D6VW16
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: November 1, 1995
Last modified: December 14, 2011
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Peptidase families

Classification of peptidase families and list of entries

SIMILARITY comments

Index of protein domains and families

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