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Protein

Phospholipase A-2-activating protein

Gene

Plaa

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the maintenance of ubiquitin levels.By similarity

GO - Molecular functioni

  • phospholipase A2 activator activity Source: MGI

GO - Biological processi

  • inflammatory response Source: MGI
  • positive regulation of catalytic activity Source: GOC
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase A-2-activating protein
Short name:
PLA2P
Short name:
PLAP
Gene namesi
Name:Plaa
Synonyms:Plap
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:104810. Plaa.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Phospholipase A-2-activating proteinPRO_0000051131Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei50 – 501PhosphoserineBy similarity
Modified residuei529 – 5291N6-acetyllysineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP27612.
MaxQBiP27612.
PaxDbiP27612.
PRIDEiP27612.

PTM databases

iPTMnetiP27612.
PhosphoSiteiP27612.

Expressioni

Inductioni

In smooth muscle and endothelial cells by leukotriene D4, by tumor necrosis factor in endothelial cells and by uric acid crystals in macrophages.

Gene expression databases

BgeeiP27612.
CleanExiMM_PLAA.
ExpressionAtlasiP27612. baseline and differential.
GenevisibleiP27612. MM.

Interactioni

Subunit structurei

Interacts with ubiquitin. Interacts with VCP (By similarity).By similarity

Protein-protein interaction databases

BioGridi202224. 3 interactions.
IntActiP27612. 1 interaction.
MINTiMINT-220705.
STRINGi10090.ENSMUSP00000102724.

Structurei

3D structure databases

ProteinModelPortaliP27612.
SMRiP27612. Positions 6-378, 386-464, 530-794.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati17 – 5640WD 1Add
BLAST
Repeati63 – 10745WD 2Add
BLAST
Repeati110 – 14839WD 3Add
BLAST
Repeati149 – 18840WD 4Add
BLAST
Repeati190 – 22738WD 5Add
BLAST
Repeati229 – 26840WD 6Add
BLAST
Repeati270 – 30738WD 7Add
BLAST
Domaini366 – 465100PFUPROSITE-ProRule annotationAdd
BLAST
Domaini533 – 793261PULPROSITE-ProRule annotationAdd
BLAST
Repeati546 – 58843ARM 1Add
BLAST
Repeati589 – 62032ARM 2Add
BLAST
Repeati621 – 66848ARM 3Add
BLAST
Repeati669 – 71446ARM 4Add
BLAST
Repeati715 – 75440ARM 5Add
BLAST
Repeati755 – 79440ARM 6Add
BLAST

Domaini

The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus.By similarity
The PFU domain mediates interaction with ubiquitin.By similarity

Sequence similaritiesi

Belongs to the WD repeat PLAP family.Curated
Contains 6 ARM repeats.Curated
Contains 1 PFU domain.PROSITE-ProRule annotation
Contains 1 PUL domain.PROSITE-ProRule annotation
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0301. Eukaryota.
ENOG410XS67. LUCA.
GeneTreeiENSGT00550000074944.
HOGENOMiHOG000174247.
HOVERGENiHBG008204.
InParanoidiP27612.
KOiK14018.
OMAiYPRGLIA.
OrthoDBiEOG7D2FCZ.
TreeFamiTF105944.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27612-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASGASRYRL SCSLPGHELD VRGLVCCLYP PGAFVSVSRD RTTRLWAPDS
60 70 80 90 100
PNRGFTEMHC MSGHSNFVSC VCIIPSSDIY PHGLIATGGN DHNICIFSLD
110 120 130 140 150
SPMPLYILKG HKDTVCSLSS GKFGTLLSGS WDTTAKVWLN DKCMMTLQGH
160 170 180 190 200
TAAVWAVKIL PEQGLMLTGS ADKTIKLWKA GRCERTFLGH EDCVRGLAIL
210 220 230 240 250
SETEFLSCAN DASIRRWQIT GECLEVYFGH TNYIYSISVF PNSKDFVTTA
260 270 280 290 300
EDRSLRIWKH GECAQTIRLP AQSIWCCCVL ENGDIVVGAS DGIIRVFTES
310 320 330 340 350
EERTASAEEI KAFERELSQA TIDSKTGDLG DINAEQLPGR EHLSEPGTRE
360 370 380 390 400
GQTRLIRDGE RVEAYQWSVS DGRWIKIGDV VGSSGANQQT SGKVLYEGKE
410 420 430 440 450
FDYVFSIDVN EGGPSYKLPY NVSDDPWLVA YNFLQKNDLN PMFLDQVAKF
460 470 480 490 500
IIDNTKGQTL GLGNTSFSDP FTGGGRYVPG TSGPSNTVQT ADPFTGAGRY
510 520 530 540 550
MPGSAGMDTT MTGVDPFTGN SAYRSAASKT VNIYFPKKEA LTFDQANPTQ
560 570 580 590 600
ILGKLKELNG TAPEEKKLTE DDLVLLEKIL SLICNNSSEK PTAQQLQILW
610 620 630 640 650
KAINWPEDIV FPALDILRLS IKHPNVNENF CNEKGDQFSS HLINLLNPKG
660 670 680 690 700
KPANQLLALR TFCNCFVSQA GQKLMMSQRE SLMSHAIELK SGSNKNIHIA
710 720 730 740 750
LATLTLNYSV CFHKDHNIEG KAQCLSVIST ILEVVQDLEA TFRLLVALGT
760 770 780 790
LISDDSNAIQ LAKSLGVDSQ IKKYVSVSEP AKVSECCRLV LHLL
Length:794
Mass (Da):87,221
Last modified:July 27, 2011 - v4
Checksum:i7D8B1D55B07563CC
GO

Sequence cautioni

The sequence AAA39943.1 differs from that shown.Several frameshifts and contaminating sequence.Curated
The sequence described in Ref. 3 differs from that shown. Reason: Frameshift at position 697. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti60 – 601C → Y in AAA39943 (PubMed:19468303).Curated
Sequence conflicti70 – 701C → Y in AAA39943 (PubMed:19468303).Curated
Sequence conflicti300 – 3012SE → AD (PubMed:7665086).Curated
Sequence conflicti528 – 5281S → T in BAC36064 (PubMed:16141072).Curated
Sequence conflicti546 – 5461A → T (PubMed:7665086).Curated
Sequence conflicti593 – 5931A → R (PubMed:7665086).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075934 mRNA. Translation: BAC36064.1.
AL732597 Genomic DNA. Translation: CAM19635.1.
CH466527 Genomic DNA. Translation: EDL30936.1.
BC139355 mRNA. Translation: AAI39356.1.
BC139356 mRNA. Translation: AAI39357.1.
BC139773 mRNA. Translation: AAI39774.1.
M57958 mRNA. Translation: AAA39943.1. Sequence problems.
CCDSiCCDS18359.1.
PIRiA40963.
RefSeqiNP_766283.2. NM_172695.2.
UniGeneiMm.490101.
Mm.491195.

Genome annotation databases

EnsembliENSMUST00000107107; ENSMUSP00000102724; ENSMUSG00000028577.
GeneIDi18786.
KEGGimmu:18786.
UCSCiuc008tsd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK075934 mRNA. Translation: BAC36064.1.
AL732597 Genomic DNA. Translation: CAM19635.1.
CH466527 Genomic DNA. Translation: EDL30936.1.
BC139355 mRNA. Translation: AAI39356.1.
BC139356 mRNA. Translation: AAI39357.1.
BC139773 mRNA. Translation: AAI39774.1.
M57958 mRNA. Translation: AAA39943.1. Sequence problems.
CCDSiCCDS18359.1.
PIRiA40963.
RefSeqiNP_766283.2. NM_172695.2.
UniGeneiMm.490101.
Mm.491195.

3D structure databases

ProteinModelPortaliP27612.
SMRiP27612. Positions 6-378, 386-464, 530-794.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202224. 3 interactions.
IntActiP27612. 1 interaction.
MINTiMINT-220705.
STRINGi10090.ENSMUSP00000102724.

PTM databases

iPTMnetiP27612.
PhosphoSiteiP27612.

Proteomic databases

EPDiP27612.
MaxQBiP27612.
PaxDbiP27612.
PRIDEiP27612.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000107107; ENSMUSP00000102724; ENSMUSG00000028577.
GeneIDi18786.
KEGGimmu:18786.
UCSCiuc008tsd.1. mouse.

Organism-specific databases

CTDi9373.
MGIiMGI:104810. Plaa.

Phylogenomic databases

eggNOGiKOG0301. Eukaryota.
ENOG410XS67. LUCA.
GeneTreeiENSGT00550000074944.
HOGENOMiHOG000174247.
HOVERGENiHBG008204.
InParanoidiP27612.
KOiK14018.
OMAiYPRGLIA.
OrthoDBiEOG7D2FCZ.
TreeFamiTF105944.

Miscellaneous databases

ChiTaRSiPlaa. mouse.
PROiP27612.
SOURCEiSearch...

Gene expression databases

BgeeiP27612.
CleanExiMM_PLAA.
ExpressionAtlasiP27612. baseline and differential.
GenevisibleiP27612. MM.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR015155. PLAA_fam_Ub-bd_PFU.
IPR013535. PUL_dom.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR017986. WD40_repeat_dom.
[Graphical view]
PfamiPF09070. PFU. 1 hit.
PF08324. PUL. 1 hit.
PF00400. WD40. 6 hits.
[Graphical view]
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS51394. PFU. 1 hit.
PS51396. PUL. 1 hit.
PS50082. WD_REPEATS_2. 3 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  5. Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-312.
  6. "Cloning of a rat cDNA encoding a protein with high homology to mouse phospholipase A2-activating protein."
    Wang H., Lemasters J.J., Herman B.
    Gene 161:237-241(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, Spleen and Testis.
  8. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-529, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiPLAP_MOUSE
AccessioniPrimary (citable) accession number: P27612
Secondary accession number(s): A4QPD6, Q8C6C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 144 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.