P27612 (PLAP_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phospholipase A-2-activating protein
Short name=PLA2P
Short name=PLAP

Gene names

Name:	Plaa
Synonyms:	Plap

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	794 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in the maintenance of ubiquitin levels By similarity.
Subunit structure	Interacts with ubiquitin. Interacts with VCP By similarity.
Induction	In smooth muscle and endothelial cells by leukotriene D4, by tumor necrosis factor in endothelial cells and by uric acid crystals in macrophages.
Domain	The PUL domain is composed of 6 armadillo-like repeats and mediates the interaction with VCP C-terminus By similarity. The PFU domain mediates interaction with ubiquitin By similarity.
Sequence similarities	Belongs to the WD repeat PLAP family. Contains 6 ARM repeats. Contains 1 PFU domain. Contains 1 PUL domain. Contains 7 WD repeats.
Sequence caution	The sequence AAA39943.1 differs from that shown. Reason: Several frameshifts and contaminating sequence. The sequence described in Ref.3 differs from that shown. Reason: Frameshift at position 697.

Ontologies

Keywords
Domain	Repeat WD repeat
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	inflammatory response Inferred from direct assay Ref.5. Source: MGI
Molecular function	phospholipase A2 activator activity Inferred from direct assay Ref.5. Source: MGI protein binding Inferred from physical interaction. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 794	794	Phospholipase A-2-activating protein		PRO_0000051131
Regions
	Repeat	17 – 56	40	WD 1
	Repeat	63 – 107	45	WD 2
	Repeat	110 – 148	39	WD 3
	Repeat	149 – 188	40	WD 4
	Repeat	190 – 227	38	WD 5
	Repeat	229 – 268	40	WD 6
	Repeat	270 – 307	38	WD 7
	Domain	366 – 465	100	PFU
	Domain	533 – 793	261	PUL
	Repeat	546 – 588	43	ARM 1
	Repeat	589 – 620	32	ARM 2
	Repeat	621 – 668	48	ARM 3
	Repeat	669 – 714	46	ARM 4
	Repeat	715 – 754	40	ARM 5
	Repeat	755 – 794	40	ARM 6
Amino acid modifications
	Modified residue	50	1	Phosphoserine By similarity
	Modified residue	529	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	60	1	C → Y in AAA39943. Ref.2
	Sequence conflict	70	1	C → Y in AAA39943. Ref.2
	Sequence conflict	300 – 301	2	SE → AD Ref.6
	Sequence conflict	528	1	S → T in BAC36064. Ref.1
	Sequence conflict	546	1	A → T Ref.6
	Sequence conflict	593	1	A → R Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P27612 [UniParc]. Last modified July 27, 2011. Version 4. Checksum: 7D8B1D55B07563CC	FASTA	794	87,221
	10 20 30 40 50 60 MASGASRYRL SCSLPGHELD VRGLVCCLYP PGAFVSVSRD RTTRLWAPDS PNRGFTEMHC 70 80 90 100 110 120 MSGHSNFVSC VCIIPSSDIY PHGLIATGGN DHNICIFSLD SPMPLYILKG HKDTVCSLSS 130 140 150 160 170 180 GKFGTLLSGS WDTTAKVWLN DKCMMTLQGH TAAVWAVKIL PEQGLMLTGS ADKTIKLWKA 190 200 210 220 230 240 GRCERTFLGH EDCVRGLAIL SETEFLSCAN DASIRRWQIT GECLEVYFGH TNYIYSISVF 250 260 270 280 290 300 PNSKDFVTTA EDRSLRIWKH GECAQTIRLP AQSIWCCCVL ENGDIVVGAS DGIIRVFTES 310 320 330 340 350 360 EERTASAEEI KAFERELSQA TIDSKTGDLG DINAEQLPGR EHLSEPGTRE GQTRLIRDGE 370 380 390 400 410 420 RVEAYQWSVS DGRWIKIGDV VGSSGANQQT SGKVLYEGKE FDYVFSIDVN EGGPSYKLPY 430 440 450 460 470 480 NVSDDPWLVA YNFLQKNDLN PMFLDQVAKF IIDNTKGQTL GLGNTSFSDP FTGGGRYVPG 490 500 510 520 530 540 TSGPSNTVQT ADPFTGAGRY MPGSAGMDTT MTGVDPFTGN SAYRSAASKT VNIYFPKKEA 550 560 570 580 590 600 LTFDQANPTQ ILGKLKELNG TAPEEKKLTE DDLVLLEKIL SLICNNSSEK PTAQQLQILW 610 620 630 640 650 660 KAINWPEDIV FPALDILRLS IKHPNVNENF CNEKGDQFSS HLINLLNPKG KPANQLLALR 670 680 690 700 710 720 TFCNCFVSQA GQKLMMSQRE SLMSHAIELK SGSNKNIHIA LATLTLNYSV CFHKDHNIEG 730 740 750 760 770 780 KAQCLSVIST ILEVVQDLEA TFRLLVALGT LISDDSNAIQ LAKSLGVDSQ IKKYVSVSEP 790 AKVSECCRLV LHLL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y. Science 309:1559-1563(2005) [PubMed: 16141072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J.
[2]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed: 19468303] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6.
[3]	Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[5]	"Cloning of a phospholipase A2-activating protein." Clark M.A., Oezguer L.E., Conway T.M., Dispoto J., Crooke S.T., Bomalaski J.S. Proc. Natl. Acad. Sci. U.S.A. 88:5418-5422(1991) [PubMed: 2052621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-312.
[6]	"Cloning of a rat cDNA encoding a protein with high homology to mouse phospholipase A2-activating protein." Wang H., Lemasters J.J., Herman B. Gene 161:237-241(1995) [PubMed: 7665086] [Abstract] Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AK075934 mRNA. Translation: BAC36064.1. AL732597 Genomic DNA. Translation: CAM19635.1. CH466527 Genomic DNA. Translation: EDL30936.1. BC139355 mRNA. Translation: AAI39356.1. BC139356 mRNA. Translation: AAI39357.1. BC139773 mRNA. Translation: AAI39774.1. M57958 mRNA. Translation: AAA39943.1. Sequence problems.
IPI	IPI00226234.
PIR	A40963.
RefSeq	NP_766283.2. NM_172695.2.
UniGene	Mm.370954. Mm.485102.
3D structure databases
ProteinModelPortal	P27612.
SMR	P27612. Positions 6-306, 386-794.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-220705.
STRING	P27612.
PTM databases
PhosphoSite	P27612.
Proteomic databases
PRIDE	P27612.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000107107; ENSMUSP00000102724; ENSMUSG00000028577.
GeneID	18786.
KEGG	mmu:18786.
Organism-specific databases
CTD	9373.
MGI	MGI:104810. Plaa.
Phylogenomic databases
eggNOG	roNOG04126.
GeneTree	ENSGT00550000074944.
HOGENOM	HBG395734.
HOVERGEN	HBG008204.
InParanoid	P27612.
OrthoDB	EOG4DJJVW.
PhylomeDB	P27612.
Gene expression databases
ArrayExpress	P27612.
Bgee	P27612.
CleanEx	MM_PLAA.
Genevestigator	P27612.
GermOnline	ENSMUSG00000028577. Mus musculus.
Family and domain databases
InterPro	IPR015155. PLAA_fam_Ub-bd_PFU. IPR013535. PUL. IPR015943. WD40/YVTN_repeat-like_dom. IPR001680. WD40_repeat. IPR011046. WD40_repeat-like_dom. IPR017986. WD40_repeat_dom. [Graphical view]
Gene3D	G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K14018.
Pfam	PF09070. PFU. 1 hit. PF08324. PUL. 1 hit. PF00400. WD40. 7 hits. [Graphical view]
SMART	SM00320. WD40. 7 hits. [Graphical view]
SUPFAM	SSF50978. WD40_like. 1 hit.
PROSITE	PS50176. ARM_REPEAT. False negative. PS51394. PFU. 1 hit. PS51396. PUL. 1 hit. PS00678. WD_REPEATS_1. False negative. PS50082. WD_REPEATS_2. 3 hits. PS50294. WD_REPEATS_REGION. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	295052.
SOURCE	Search...

Entry information

Entry name

PLAP_MOUSE

Accession

Primary (citable) accession number: P27612
Secondary accession number(s): A4QPD6, Q8C6C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	July 27, 2011
Last modified:	January 25, 2012
This is version 104 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program