Reviewed, UniProtKB/Swiss-Prot P27608 (AROF_TOBAC)

Names and origin

Protein names

Recommended name:
    Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic
    EC=2.5.1.54
Alternative name(s):
    Phospho-2-keto-3-deoxyheptonate aldolase 1
    3-deoxy-D-arabino-heptulosonate 7-phosphate synthase 1
    DAHP synthase 1

Gene names

Name:

DHAPS-1

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	542 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-erythrose 4-phosphate + H2O = 3-deoxy-D-arabino-hept-2-ulosonate 7-phosphate + phosphate.
Enzyme regulation	Activation by tryptophan (a hysteretic factor).
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 1/7.
Subcellular location	Plastid › chloroplast.
Tissue specificity	Leaves, stems, and petals.
Induction	By the herbicide glyphosate and wounding.
Sequence similarities	Belongs to the class-II DAHP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-7-phosphoheptulonate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 76	76	Chloroplast By similarity
	Chain	77 – 542	466	Phospho-2-dehydro-3-deoxyheptonate aldolase 1, chloroplastic		PRO_0000002304

Sequences

	Sequence		Length	Mass (Da)
	P27608-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 4C166391ADBA7D51	FASTA	542	59,975
	10 20 30 40 50 60 MALSSSSTTN SLLPNKSQLV QNQSLLPSPL KNVSFTTNST KPVRFVQPIS AIHSSDSSKN 70 80 90 100 110 120 PIVSDKPSSK PSPPAATVTA AATTVTKTEW TVESWKSKKA LQLPEYPNQE ELQSVLKTIE 130 140 150 160 170 180 EFPPIVFAGE ARSLEERLGE AAMGRAFLLQ GGDCAESFKE FNANNIRDTF RILLQMGAVL 190 200 210 220 230 240 MFGGQMPVIK VGRMAGQFAK PRSDNFEEKN GVKLPSYRGD NVNGDAFDAK SRTPDPQRLI 250 260 270 280 290 300 RAYCQSAATL NLLRAFATGG YAAMQRINQW NLDFTEHSEQ GDRYRELANR VDEALGFMAA 310 320 330 340 350 360 AGLTVDHPIM KTTEFWTSHE CLLLPYEQSL TRLDSTSGLY YDCSAHFIWV GERTRQLDGA 370 380 390 400 410 420 HVEFLRGVAN PLGIKVSDKM DPSALVKLIE ILNPDNKAGR ITIITRMGAE NMRVKLPHLI 430 440 450 460 470 480 RAVRRAGQIV TWVSDPMHGN TIKAPCGLKT RPFDSIRAEV RAFFDVHEQE GSHPGGVHLE 490 500 510 520 530 540 MTGQNVTECI GGSRTVTFDD LSSRYHTHCD PRLNASQSLE LAFIIAERLR KRRLGSQNVL GQ

References

[1]

"Cloning and nucleotide sequence of a complementary DNA encoding 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from tobacco." Wang Y., Herrmann K.M., Weller S.C., Goldsbrough P.B. Plant Physiol. 97:847-848(1991) [PubMed: 16668482] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Wisconsin 38.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M64261 mRNA. Translation: AAA34068.1.
3D structure databases
SMR	P27608. Positions 89-531.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.54. 298.
Family and domain databases
InterPro	IPR002480. DAHP_synth_2. [Graphical view]
PANTHER	PTHR21337. DAHP_synth_2. 1 hit.
Pfam	PF01474. DAHP_synth_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01358. DAHP_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

AROF_TOBAC

Accession

Primary (citable) accession number: P27608

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	February 9, 2010
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)