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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi: IMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes IMP from hypoxanthine.
Proteins known to be involved in this subpathway in this organism are:
  1. Hypoxanthine-guanine phosphoribosyltransferase (Hprt1)
This subpathway is part of the pathway IMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes IMP from hypoxanthine, the pathway IMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei69GMPBy similarity1
Active sitei138Proton acceptorBy similarity1
Binding sitei166GMPBy similarity1
Metal bindingi194MagnesiumBy similarity1
Binding sitei194GMP; via carbonyl oxygenBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi134 – 142GMPBy similarity9
Nucleotide bindingi186 – 188GMPBy similarity3

GO - Molecular functioni

GO - Biological processi

  • adenine salvage Source: GO_Central
  • brain development Source: RGD
  • cellular response to insulin stimulus Source: RGD
  • GMP catabolic process Source: UniProtKB
  • GMP salvage Source: GO_Central
  • guanine salvage Source: UniProtKB
  • hypoxanthine metabolic process Source: RGD
  • hypoxanthine salvage Source: UniProtKB
  • IMP metabolic process Source: UniProtKB
  • IMP salvage Source: GO_Central
  • spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-74217. Purine salvage.
SABIO-RKP27605.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:Hprt1
Synonyms:Hprt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome X

Organism-specific databases

RGDi2826. Hprt1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001395912 – 218Hypoxanthine-guanine phosphoribosyltransferaseAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei103N6-acetyllysineBy similarity1
Cross-linki115Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Modified residuei142PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP27605.
PRIDEiP27605.

2D gel databases

World-2DPAGE0004:P27605.

PTM databases

iPTMnetiP27605.
PhosphoSitePlusiP27605.

Expressioni

Gene expression databases

BgeeiENSRNOG00000031367.
ExpressionAtlasiP27605. differential.
GenevisibleiP27605. RN.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP27605. 2 interactors.
MINTiMINT-4579300.
STRINGi10116.ENSRNOP00000043388.

Structurei

3D structure databases

ProteinModelPortaliP27605.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG3367. Eukaryota.
COG0634. LUCA.
GeneTreeiENSGT00390000017323.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiP27605.
KOiK00760.
OMAiDIAYVGF.
OrthoDBiEOG091G0K6K.
PhylomeDBiP27605.
TreeFamiTF313367.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27605-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEHFR
210
DLNHVCVISE SGKAKYKA
Length:218
Mass (Da):24,477
Last modified:August 1, 1992 - v1
Checksum:i2683FC1444FE0B5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63983 mRNA. Translation: AAA41350.1.
S79292 mRNA. Translation: AAB21288.1.
X62085 mRNA. Translation: CAA43997.1.
AF001282
, AF001278, AF009655, AF009656, AF001279, AF001280, AF001281 Genomic DNA. Translation: AAB65640.1.
BC098629 mRNA. Translation: AAH98629.1.
U06049 Genomic DNA. Translation: AAA56887.1.
PIRiS18140.
RefSeqiNP_036715.1. NM_012583.2.
XP_008771881.1. XM_008773659.2.
UniGeneiRn.47.

Genome annotation databases

EnsembliENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367.
ENSRNOT00000065935; ENSRNOP00000062740; ENSRNOG00000048561.
GeneIDi103689983.
24465.
KEGGirno:103689983.
rno:24465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63983 mRNA. Translation: AAA41350.1.
S79292 mRNA. Translation: AAB21288.1.
X62085 mRNA. Translation: CAA43997.1.
AF001282
, AF001278, AF009655, AF009656, AF001279, AF001280, AF001281 Genomic DNA. Translation: AAB65640.1.
BC098629 mRNA. Translation: AAH98629.1.
U06049 Genomic DNA. Translation: AAA56887.1.
PIRiS18140.
RefSeqiNP_036715.1. NM_012583.2.
XP_008771881.1. XM_008773659.2.
UniGeneiRn.47.

3D structure databases

ProteinModelPortaliP27605.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP27605. 2 interactors.
MINTiMINT-4579300.
STRINGi10116.ENSRNOP00000043388.

PTM databases

iPTMnetiP27605.
PhosphoSitePlusiP27605.

2D gel databases

World-2DPAGE0004:P27605.

Proteomic databases

PaxDbiP27605.
PRIDEiP27605.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367.
ENSRNOT00000065935; ENSRNOP00000062740; ENSRNOG00000048561.
GeneIDi103689983.
24465.
KEGGirno:103689983.
rno:24465.

Organism-specific databases

CTDi3251.
RGDi2826. Hprt1.

Phylogenomic databases

eggNOGiKOG3367. Eukaryota.
COG0634. LUCA.
GeneTreeiENSGT00390000017323.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiP27605.
KOiK00760.
OMAiDIAYVGF.
OrthoDBiEOG091G0K6K.
PhylomeDBiP27605.
TreeFamiTF313367.

Enzyme and pathway databases

UniPathwayiUPA00591; UER00648.
ReactomeiR-RNO-74217. Purine salvage.
SABIO-RKP27605.

Miscellaneous databases

PROiP27605.

Gene expression databases

BgeeiENSRNOG00000031367.
ExpressionAtlasiP27605. differential.
GenevisibleiP27605. RN.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHPRT_RAT
AccessioniPrimary (citable) accession number: P27605
Secondary accession number(s): P70469, Q4KMC5, Q62926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 30, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.