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P27605

- HPRT_RAT

UniProt

P27605 - HPRT_RAT

Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway By similarity.By similarity

    Catalytic activityi

    IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
    GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

    Cofactori

    Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei69 – 691GMPBy similarity
    Active sitei138 – 1381Proton acceptorBy similarity
    Binding sitei166 – 1661GMPBy similarity
    Metal bindingi194 – 1941MagnesiumBy similarity
    Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi134 – 1429GMPBy similarity
    Nucleotide bindingi186 – 1883GMPBy similarity

    GO - Molecular functioni

    1. guanine phosphoribosyltransferase activity Source: UniProtKB
    2. hypoxanthine phosphoribosyltransferase activity Source: RGD
    3. magnesium ion binding Source: RefGenome
    4. nucleotide binding Source: UniProtKB-KW

    GO - Biological processi

    1. adenine salvage Source: RefGenome
    2. brain development Source: RGD
    3. cellular response to insulin stimulus Source: RGD
    4. GMP catabolic process Source: UniProtKB
    5. GMP salvage Source: RefGenome
    6. guanine salvage Source: UniProtKB
    7. hypoxanthine metabolic process Source: RGD
    8. hypoxanthine salvage Source: UniProtKB
    9. IMP metabolic process Source: UniProtKB
    10. IMP salvage Source: RefGenome
    11. purine ribonucleoside salvage Source: RefGenome
    12. spermatogenesis Source: RGD

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Keywords - Ligandi

    Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_221255. Purine salvage.
    SABIO-RKP27605.
    UniPathwayiUPA00591; UER00648.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
    Short name:
    HGPRT
    Short name:
    HGPRTase
    Gene namesi
    Name:Hprt1
    Synonyms:Hprt
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome X

    Organism-specific databases

    RGDi2826. Hprt1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: RefGenome

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139591Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei103 – 1031N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    PaxDbiP27605.
    PRIDEiP27605.

    2D gel databases

    World-2DPAGE0004:P27605.

    PTM databases

    PhosphoSiteiP27605.

    Expressioni

    Gene expression databases

    GenevestigatoriP27605.

    Interactioni

    Subunit structurei

    Homotetramer.By similarity

    Protein-protein interaction databases

    IntActiP27605. 2 interactions.
    MINTiMINT-4579300.

    Structurei

    3D structure databases

    ProteinModelPortaliP27605.
    SMRiP27605. Positions 5-218.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0634.
    GeneTreeiENSGT00390000017323.
    HOGENOMiHOG000236521.
    HOVERGENiHBG000242.
    InParanoidiP27605.
    KOiK00760.
    OMAiAREIMKG.
    OrthoDBiEOG7673CK.
    PhylomeDBiP27605.
    TreeFamiTF313367.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    InterProiIPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SUPFAMiSSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
    PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27605-1 [UniParc]FASTAAdd to Basket

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    MSTLSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA    50
    RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI 100
    RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV 150
    KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEHFR 200
    DLNHVCVISE SGKAKYKA 218
    Length:218
    Mass (Da):24,477
    Last modified:August 1, 1992 - v1
    Checksum:i2683FC1444FE0B5E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63983 mRNA. Translation: AAA41350.1.
    S79292 mRNA. Translation: AAB21288.1.
    X62085 mRNA. Translation: CAA43997.1.
    AF001282
    , AF001278, AF009655, AF009656, AF001279, AF001280, AF001281 Genomic DNA. Translation: AAB65640.1.
    BC098629 mRNA. Translation: AAH98629.1.
    U06049 Genomic DNA. Translation: AAA56887.1.
    PIRiS18140.
    RefSeqiNP_036715.1. NM_012583.2.
    UniGeneiRn.47.

    Genome annotation databases

    EnsembliENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367.
    GeneIDi24465.
    KEGGirno:24465.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63983 mRNA. Translation: AAA41350.1 .
    S79292 mRNA. Translation: AAB21288.1 .
    X62085 mRNA. Translation: CAA43997.1 .
    AF001282
    , AF001278 , AF009655 , AF009656 , AF001279 , AF001280 , AF001281 Genomic DNA. Translation: AAB65640.1 .
    BC098629 mRNA. Translation: AAH98629.1 .
    U06049 Genomic DNA. Translation: AAA56887.1 .
    PIRi S18140.
    RefSeqi NP_036715.1. NM_012583.2.
    UniGenei Rn.47.

    3D structure databases

    ProteinModelPortali P27605.
    SMRi P27605. Positions 5-218.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P27605. 2 interactions.
    MINTi MINT-4579300.

    PTM databases

    PhosphoSitei P27605.

    2D gel databases

    World-2DPAGE 0004:P27605.

    Proteomic databases

    PaxDbi P27605.
    PRIDEi P27605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000045153 ; ENSRNOP00000043388 ; ENSRNOG00000031367 .
    GeneIDi 24465.
    KEGGi rno:24465.

    Organism-specific databases

    CTDi 3251.
    RGDi 2826. Hprt1.

    Phylogenomic databases

    eggNOGi COG0634.
    GeneTreei ENSGT00390000017323.
    HOGENOMi HOG000236521.
    HOVERGENi HBG000242.
    InParanoidi P27605.
    KOi K00760.
    OMAi AREIMKG.
    OrthoDBi EOG7673CK.
    PhylomeDBi P27605.
    TreeFami TF313367.

    Enzyme and pathway databases

    UniPathwayi UPA00591 ; UER00648 .
    Reactomei REACT_221255. Purine salvage.
    SABIO-RK P27605.

    Miscellaneous databases

    NextBioi 603395.
    PROi P27605.

    Gene expression databases

    Genevestigatori P27605.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    InterProi IPR005904. Hxn_phspho_trans.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
    PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Rat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis."
      Chiaverotti T.A., Battula N., Monnat R.J. Jr.
      Genomics 11:1158-1160(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Rat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis."
      Chiaverotti T.A., Battula N., Monnat R.J. Jr.
      Adv. Exp. Med. Biol. 309B:117-120(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The gene encoding hypoxanthine-guanine phosphoribosyltransferase as target for mutational analysis: PCR cloning and sequencing of the cDNA from the rat."
      Jansen J.G., Vrieling H., van Zeeland A.A., Mohn G.R.
      Mutat. Res. 266:105-116(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    4. "DNA sequence flanking the protein coding regions of the rat Hprt gene."
      Chen T., Mittelstaedt R.A., Heflich R.H.
      Mutat. Res. 382:79-80(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Fischer 344.
      Tissue: Spleen.
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Thymus.
    6. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
      Submitted (SEP-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 35-45; 74-83; 92-101; 116-141; 157-166 AND 171-200, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Sprague-Dawley.
      Tissue: Brain, Hippocampus and Spinal cord.
    7. "Analysis of in vivo mutation in exon 8 of the rat hprt gene."
      Mittelstaedt R.A., Heflich R.H.
      Mutat. Res. 311:139-148(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-203.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiHPRT_RAT
    AccessioniPrimary (citable) accession number: P27605
    Secondary accession number(s): P70469, Q4KMC5, Q62926
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3