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P27605

- HPRT_RAT

UniProt

P27605 - HPRT_RAT

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Protein

Hypoxanthine-guanine phosphoribosyltransferase

Gene

Hprt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Converts guanine to guanosine monophosphate, and hypoxanthine to inosine monophosphate. Transfers the 5-phosphoribosyl group from 5-phosphoribosylpyrophosphate onto the purine. Plays a central role in the generation of purine nucleotides through the purine salvage pathway (By similarity).By similarity

Catalytic activityi

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate.
GMP + diphosphate = guanine + 5-phospho-alpha-D-ribose 1-diphosphate.

Cofactori

Mg2+By similarityNote: Binds 2 magnesium ions per subunit. The magnesium ions are essentially bound to the substrate and have few direct interactions with the protein.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei69 – 691GMPBy similarity
Active sitei138 – 1381Proton acceptorBy similarity
Binding sitei166 – 1661GMPBy similarity
Metal bindingi194 – 1941MagnesiumBy similarity
Binding sitei194 – 1941GMP; via carbonyl oxygenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi134 – 1429GMPBy similarity
Nucleotide bindingi186 – 1883GMPBy similarity

GO - Molecular functioni

  1. guanine phosphoribosyltransferase activity Source: UniProtKB
  2. hypoxanthine phosphoribosyltransferase activity Source: RGD
  3. magnesium ion binding Source: RefGenome
  4. nucleotide binding Source: UniProtKB-KW

GO - Biological processi

  1. adenine salvage Source: RefGenome
  2. brain development Source: RGD
  3. cellular response to insulin stimulus Source: RGD
  4. GMP catabolic process Source: UniProtKB
  5. GMP salvage Source: RefGenome
  6. guanine salvage Source: UniProtKB
  7. hypoxanthine metabolic process Source: RGD
  8. hypoxanthine salvage Source: UniProtKB
  9. IMP metabolic process Source: UniProtKB
  10. IMP salvage Source: RefGenome
  11. purine ribonucleoside salvage Source: RefGenome
  12. spermatogenesis Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_221255. Purine salvage.
SABIO-RKP27605.
UniPathwayiUPA00591; UER00648.

Names & Taxonomyi

Protein namesi
Recommended name:
Hypoxanthine-guanine phosphoribosyltransferase (EC:2.4.2.8)
Short name:
HGPRT
Short name:
HGPRTase
Gene namesi
Name:Hprt1
Synonyms:Hprt
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome X

Organism-specific databases

RGDi2826. Hprt1.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 218217Hypoxanthine-guanine phosphoribosyltransferasePRO_0000139591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei103 – 1031N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiP27605.
PRIDEiP27605.

2D gel databases

World-2DPAGE0004:P27605.

PTM databases

PhosphoSiteiP27605.

Expressioni

Gene expression databases

ExpressionAtlasiP27605. baseline.
GenevestigatoriP27605.

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

IntActiP27605. 2 interactions.
MINTiMINT-4579300.

Structurei

3D structure databases

ProteinModelPortaliP27605.
SMRiP27605. Positions 5-218.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0634.
GeneTreeiENSGT00390000017323.
HOGENOMiHOG000236521.
HOVERGENiHBG000242.
InParanoidiP27605.
KOiK00760.
OMAiAREIMKG.
OrthoDBiEOG7673CK.
PhylomeDBiP27605.
TreeFamiTF313367.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
InterProiIPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF00156. Pribosyltran. 1 hit.
[Graphical view]
SUPFAMiSSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR01203. HGPRTase. 1 hit.
PROSITEiPS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27605-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSTLSPSVVI SDDEPGYDLD LFCIPNHYAE DLEKVFIPHG LIMDRTERLA
60 70 80 90 100
RDVMKEMGGH HIVALCVLKG GYKFFADLLD YIKALNRNSD RSIPMTVDFI
110 120 130 140 150
RLKSYCNDQS TGDIKVIGGD DLSTLTGKNV LIVEDIIDTG KTMQTLLSLV
160 170 180 190 200
KQYSPKMVKV ASLLVKRTSR SVGYRPDFVG FEIPDKFVVG YALDYNEHFR
210
DLNHVCVISE SGKAKYKA
Length:218
Mass (Da):24,477
Last modified:August 1, 1992 - v1
Checksum:i2683FC1444FE0B5E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63983 mRNA. Translation: AAA41350.1.
S79292 mRNA. Translation: AAB21288.1.
X62085 mRNA. Translation: CAA43997.1.
AF001282
, AF001278, AF009655, AF009656, AF001279, AF001280, AF001281 Genomic DNA. Translation: AAB65640.1.
BC098629 mRNA. Translation: AAH98629.1.
U06049 Genomic DNA. Translation: AAA56887.1.
PIRiS18140.
RefSeqiNP_036715.1. NM_012583.2.
XP_008771881.1. XM_008773659.1.
UniGeneiRn.47.

Genome annotation databases

EnsembliENSRNOT00000045153; ENSRNOP00000043388; ENSRNOG00000031367.
GeneIDi103689983.
24465.
KEGGirno:24465.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63983 mRNA. Translation: AAA41350.1 .
S79292 mRNA. Translation: AAB21288.1 .
X62085 mRNA. Translation: CAA43997.1 .
AF001282
, AF001278 , AF009655 , AF009656 , AF001279 , AF001280 , AF001281 Genomic DNA. Translation: AAB65640.1 .
BC098629 mRNA. Translation: AAH98629.1 .
U06049 Genomic DNA. Translation: AAA56887.1 .
PIRi S18140.
RefSeqi NP_036715.1. NM_012583.2.
XP_008771881.1. XM_008773659.1.
UniGenei Rn.47.

3D structure databases

ProteinModelPortali P27605.
SMRi P27605. Positions 5-218.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P27605. 2 interactions.
MINTi MINT-4579300.

PTM databases

PhosphoSitei P27605.

2D gel databases

World-2DPAGE 0004:P27605.

Proteomic databases

PaxDbi P27605.
PRIDEi P27605.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000045153 ; ENSRNOP00000043388 ; ENSRNOG00000031367 .
GeneIDi 103689983.
24465.
KEGGi rno:24465.

Organism-specific databases

CTDi 3251.
RGDi 2826. Hprt1.

Phylogenomic databases

eggNOGi COG0634.
GeneTreei ENSGT00390000017323.
HOGENOMi HOG000236521.
HOVERGENi HBG000242.
InParanoidi P27605.
KOi K00760.
OMAi AREIMKG.
OrthoDBi EOG7673CK.
PhylomeDBi P27605.
TreeFami TF313367.

Enzyme and pathway databases

UniPathwayi UPA00591 ; UER00648 .
Reactomei REACT_221255. Purine salvage.
SABIO-RK P27605.

Miscellaneous databases

NextBioi 603395.
PROi P27605.

Gene expression databases

ExpressionAtlasi P27605. baseline.
Genevestigatori P27605.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
InterProi IPR005904. Hxn_phspho_trans.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF00156. Pribosyltran. 1 hit.
[Graphical view ]
SUPFAMi SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR01203. HGPRTase. 1 hit.
PROSITEi PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Rat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis."
    Chiaverotti T.A., Battula N., Monnat R.J. Jr.
    Genomics 11:1158-1160(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Rat hypoxanthine phosphoribosyltransferase cDNA cloning and sequence analysis."
    Chiaverotti T.A., Battula N., Monnat R.J. Jr.
    Adv. Exp. Med. Biol. 309B:117-120(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The gene encoding hypoxanthine-guanine phosphoribosyltransferase as target for mutational analysis: PCR cloning and sequencing of the cDNA from the rat."
    Jansen J.G., Vrieling H., van Zeeland A.A., Mohn G.R.
    Mutat. Res. 266:105-116(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  4. "DNA sequence flanking the protein coding regions of the rat Hprt gene."
    Chen T., Mittelstaedt R.A., Heflich R.H.
    Mutat. Res. 382:79-80(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Fischer 344.
    Tissue: Spleen.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Thymus.
  6. Lubec G., Afjehi-Sadat L., Chen W.-Q., Kang S.U., Lubec S.
    Submitted (SEP-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 35-45; 74-83; 92-101; 116-141; 157-166 AND 171-200, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Brain, Hippocampus and Spinal cord.
  7. "Analysis of in vivo mutation in exon 8 of the rat hprt gene."
    Mittelstaedt R.A., Heflich R.H.
    Mutat. Res. 311:139-148(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 163-203.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiHPRT_RAT
AccessioniPrimary (citable) accession number: P27605
Secondary accession number(s): P70469, Q4KMC5, Q62926
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 26, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3