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P27601

- GNA13_MOUSE

UniProt

P27601 - GNA13_MOUSE

Protein

Guanine nucleotide-binding protein subunit alpha-13

Gene

Gna13

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi62 – 621MagnesiumBy similarity
    Metal bindingi203 – 2031MagnesiumBy similarity
    Binding sitei349 – 3491GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi55 – 628GTPBy similarity
    Nucleotide bindingi197 – 2037GTPBy similarity
    Nucleotide bindingi222 – 2265GTPBy similarity
    Nucleotide bindingi291 – 2944GTPBy similarity

    GO - Molecular functioni

    1. D5 dopamine receptor binding Source: RefGenome
    2. G-protein beta/gamma-subunit complex binding Source: RefGenome
    3. GTPase activity Source: MGI
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. protein binding Source: IntAct
    7. signal transducer activity Source: RefGenome
    8. type 1 angiotensin receptor binding Source: RefGenome

    GO - Biological processi

    1. activation of phospholipase D activity Source: RefGenome
    2. adenylate cyclase-activating G-protein coupled receptor signaling pathway Source: MGI
    3. angiogenesis Source: MGI
    4. cell differentiation Source: MGI
    5. G-protein coupled receptor signaling pathway Source: MGI
    6. intracellular signal transduction Source: MGI
    7. in utero embryonic development Source: MGI
    8. patterning of blood vessels Source: MGI
    9. platelet activation Source: MGI
    10. positive regulation of cytosolic calcium ion concentration Source: Ensembl
    11. regulation of cell migration Source: MGI
    12. regulation of cell shape Source: MGI
    13. Rho protein signal transduction Source: MGI

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit alpha-13
    Short name:
    G alpha-13
    Short name:
    G-protein subunit alpha-13
    Gene namesi
    Name:Gna13
    Synonyms:Gna-13
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:95768. Gna13.

    Subcellular locationi

    Membrane 1 Publication; Lipid-anchor 1 Publication. Melanosome By similarity. Cytoplasm 1 Publication. Nucleus 1 Publication
    Note: Cytoplasmic in adult somatic cells, but mainly nuclear in spermatids in the testes. Translocates from the cytoplasm to the nucleus during spermatogenesis, hence predominantly observed in the cytoplasm of round spermatids but localized in the nuclei of elongating or elongated spermatids and testicular spermatozoa.

    GO - Cellular componenti

    1. brush border membrane Source: RefGenome
    2. heterotrimeric G-protein complex Source: MGI
    3. melanosome Source: UniProtKB-SubCell
    4. nucleus Source: UniProtKB-SubCell
    5. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Cytoplasm, Membrane, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 377377Guanine nucleotide-binding protein subunit alpha-13PRO_0000203774Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi14 – 141S-palmitoyl cysteineBy similarity
    Lipidationi18 – 181S-palmitoyl cysteineBy similarity
    Modified residuei203 – 2031PhosphothreonineBy similarity

    Post-translational modificationi

    Phosphorylation on Thr-203 destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation.By similarity

    Keywords - PTMi

    Lipoprotein, Palmitate, Phosphoprotein

    Proteomic databases

    MaxQBiP27601.
    PaxDbiP27601.
    PRIDEiP27601.

    PTM databases

    PhosphoSiteiP27601.

    Expressioni

    Tissue specificityi

    Expressed in brain and testis, as well as in kidney and sperm (at protein level).1 Publication

    Gene expression databases

    ArrayExpressiP27601.
    BgeeiP27601.
    CleanExiMM_GNA13.
    GenevestigatoriP27601.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts with HAX1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane By similarity. Interacts with RGS22 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    AipO089153EBI-2255627,EBI-6935014

    Protein-protein interaction databases

    BioGridi199963. 2 interactions.
    DIPiDIP-46241N.
    IntActiP27601. 3 interactions.
    MINTiMINT-4096246.

    Structurei

    Secondary structure

    1
    377
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi49 – 546
    Helixi61 – 7212
    Helixi78 – 825
    Helixi85 – 10521
    Helixi113 – 1153
    Helixi116 – 1238
    Beta strandi127 – 1293
    Helixi130 – 1334
    Helixi139 – 15416
    Helixi156 – 1638
    Helixi164 – 1674
    Helixi174 – 1785
    Helixi181 – 1844
    Beta strandi185 – 1884
    Helixi193 – 1986
    Beta strandi204 – 21310
    Beta strandi216 – 2238
    Helixi227 – 23610
    Beta strandi242 – 2487
    Helixi251 – 2533
    Beta strandi259 – 2635
    Helixi264 – 27613
    Helixi279 – 2813
    Beta strandi284 – 2918
    Helixi293 – 2997
    Turni300 – 3023
    Helixi305 – 3073
    Helixi319 – 33113
    Turni337 – 3393
    Beta strandi343 – 3464
    Helixi352 – 37120

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SHZX-ray2.85A/D52-159[»]
    A/D333-376[»]
    1ZCBX-ray2.00A47-377[»]
    3AB3X-ray2.40A/C47-377[»]
    3CX6X-ray2.50A41-377[»]
    3CX7X-ray2.25A41-377[»]
    3CX8X-ray2.00A41-377[»]
    ProteinModelPortaliP27601.
    SMRiP27601. Positions 47-369.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27601.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(12) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG279688.
    GeneTreeiENSGT00690000101672.
    HOGENOMiHOG000038729.
    HOVERGENiHBG063184.
    InParanoidiP27601.
    KOiK04639.
    OMAiCFPGCVL.
    OrthoDBiEOG7HF1JF.
    PhylomeDBiP27601.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR000469. Gprotein_alpha_12.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00440. GPROTEINA12.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P27601-1 [UniParc]FASTAAdd to Basket

    « Hide

    MADFLPSRSV LSVCFPGCVL TNGEAEQQRK SKEIDKCLSR EKTYVKRLVK    50
    ILLLGAGESG KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV 100
    DAREKLHIPW GDNKNQLHGD KLMAFDTRAP MAAQGMVETR VFLQYLPAIR 150
    ALWEDSGIQN AYDRRREFQL GESVKYFLDN LDKLGVPDYI PSQQDILLAR 200
    RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV TSILFLVSSS 250
    EFDQVLMEDR QTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV 300
    QVVSIKDYFL EFEGDPHCLR DVQKFLVECF RGKRRDQQQR PLYHHFTTAI 350
    NTENIRLVFR DVKDTILHDN LKQLMLQ 377
    Length:377
    Mass (Da):44,055
    Last modified:August 1, 1992 - v1
    Checksum:i45A6A6DB47C707BB
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti52 – 521L → P in AAH57665. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63660 mRNA. Translation: AAA37649.1.
    BC057665 mRNA. Translation: AAH57665.1.
    M57620 mRNA. Translation: AAA63303.1.
    CCDSiCCDS25577.1.
    PIRiB41095.
    RefSeqiNP_034433.3. NM_010303.3.
    UniGeneiMm.193925.

    Genome annotation databases

    EnsembliENSMUST00000020930; ENSMUSP00000020930; ENSMUSG00000020611.
    GeneIDi14674.
    KEGGimmu:14674.
    UCSCiuc007mce.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63660 mRNA. Translation: AAA37649.1 .
    BC057665 mRNA. Translation: AAH57665.1 .
    M57620 mRNA. Translation: AAA63303.1 .
    CCDSi CCDS25577.1.
    PIRi B41095.
    RefSeqi NP_034433.3. NM_010303.3.
    UniGenei Mm.193925.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SHZ X-ray 2.85 A/D 52-159 [» ]
    A/D 333-376 [» ]
    1ZCB X-ray 2.00 A 47-377 [» ]
    3AB3 X-ray 2.40 A/C 47-377 [» ]
    3CX6 X-ray 2.50 A 41-377 [» ]
    3CX7 X-ray 2.25 A 41-377 [» ]
    3CX8 X-ray 2.00 A 41-377 [» ]
    ProteinModelPortali P27601.
    SMRi P27601. Positions 47-369.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199963. 2 interactions.
    DIPi DIP-46241N.
    IntActi P27601. 3 interactions.
    MINTi MINT-4096246.

    PTM databases

    PhosphoSitei P27601.

    Proteomic databases

    MaxQBi P27601.
    PaxDbi P27601.
    PRIDEi P27601.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000020930 ; ENSMUSP00000020930 ; ENSMUSG00000020611 .
    GeneIDi 14674.
    KEGGi mmu:14674.
    UCSCi uc007mce.1. mouse.

    Organism-specific databases

    CTDi 10672.
    MGIi MGI:95768. Gna13.

    Phylogenomic databases

    eggNOGi NOG279688.
    GeneTreei ENSGT00690000101672.
    HOGENOMi HOG000038729.
    HOVERGENi HBG063184.
    InParanoidi P27601.
    KOi K04639.
    OMAi CFPGCVL.
    OrthoDBi EOG7HF1JF.
    PhylomeDBi P27601.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Miscellaneous databases

    ChiTaRSi GNA13. mouse.
    EvolutionaryTracei P27601.
    NextBioi 286562.
    PROi P27601.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27601.
    Bgeei P27601.
    CleanExi MM_GNA13.
    Genevestigatori P27601.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR000469. Gprotein_alpha_12.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00440. GPROTEINA12.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits."
      Strathmann M.P., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 88:5582-5586(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NMRI.
      Tissue: Mammary tumor.
    3. Lubec G., Kang S.U.
      Submitted (APR-2007) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 51-61 AND 220-227, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: C57BL/6.
      Tissue: Brain.
    4. "Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
      Strathmann M., Wilkie T.M., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 228-288.
    5. "RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits."
      Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z., Sha J.
      Biol. Reprod. 79:1021-1029(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
      Tissue: Testis.

    Entry informationi

    Entry nameiGNA13_MOUSE
    AccessioniPrimary (citable) accession number: P27601
    Secondary accession number(s): Q6PF99
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3