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P27601 (GNA13_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein subunit alpha-13

Short name=G alpha-13
Short name=G-protein subunit alpha-13
Gene names
Name:Gna13
Synonyms:Gna-13
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts with HAX1. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane By similarity. Interacts with RGS22 By similarity.

Subcellular location

Membrane; Lipid-anchor. Melanosome By similarity. Cytoplasm. Nucleus. Note: Cytoplasmic in adult somatic cells, but mainly nuclear in spermatids in the testes. Translocates from the cytoplasm to the nucleus during spermatogenesis, hence predominantly observed in the cytoplasm of round spermatids but localized in the nuclei of elongating or elongated spermatids and testicular spermatozoa. Ref.5

Tissue specificity

Expressed in brain and testis, as well as in kidney and sperm (at protein level). Ref.5

Post-translational modification

Phosphorylation on Thr-203 destabilizes the heterotrimer of alpha, beta and gamma, and inhibits Rho activation By similarity.

Sequence similarities

Belongs to the G-alpha family. G(12) subfamily.

Ontologies

Keywords
   Cellular componentCytoplasm
Membrane
Nucleus
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMLipoprotein
Palmitate
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Inferred from sequence alignment Ref.4. Source: MGI

Rho protein signal transduction

Inferred from direct assay PubMed 10037795. Source: MGI

activation of phospholipase D activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

adenylate cyclase-activating G-protein coupled receptor signaling pathway

Inferred from genetic interaction PubMed 18541530. Source: MGI

angiogenesis

Inferred from mutant phenotype PubMed 8999798. Source: MGI

cell differentiation

Inferred from direct assay PubMed 9305907. Source: MGI

in utero embryonic development

Inferred from genetic interaction PubMed 12077299. Source: MGI

intracellular signal transduction

Inferred from direct assay PubMed 10037795. Source: MGI

patterning of blood vessels

Inferred from mutant phenotype PubMed 15919816. Source: MGI

platelet activation

Inferred from mutant phenotype PubMed 14528298. Source: MGI

positive regulation of cytosolic calcium ion concentration

Inferred from electronic annotation. Source: Ensembl

regulation of cell migration

Inferred from mutant phenotype PubMed 12077299. Source: MGI

regulation of cell shape

Inferred from direct assay PubMed 10037795. Source: MGI

   Cellular_componentbrush border membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

extrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Inferred from sequence alignment Ref.4. Source: MGI

melanosome

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionD5 dopamine receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

G-protein beta/gamma-subunit complex binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from sequence alignment Ref.4. Source: MGI

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

protein binding

Inferred from physical interaction PubMed 19390533. Source: IntAct

signal transducer activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

type 1 angiotensin receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

AipO089153EBI-2255627,EBI-6935014

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 377377Guanine nucleotide-binding protein subunit alpha-13
PRO_0000203774

Regions

Nucleotide binding55 – 628GTP By similarity
Nucleotide binding197 – 2037GTP By similarity
Nucleotide binding222 – 2265GTP By similarity
Nucleotide binding291 – 2944GTP By similarity

Sites

Metal binding621Magnesium By similarity
Metal binding2031Magnesium By similarity
Binding site3491GTP; via amide nitrogen By similarity

Amino acid modifications

Modified residue2031Phosphothreonine By similarity
Lipidation141S-palmitoyl cysteine By similarity
Lipidation181S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict521L → P in AAH57665. Ref.2

Secondary structure

......................................................... 377
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P27601 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 45A6A6DB47C707BB

FASTA37744,055
        10         20         30         40         50         60 
MADFLPSRSV LSVCFPGCVL TNGEAEQQRK SKEIDKCLSR EKTYVKRLVK ILLLGAGESG 

        70         80         90        100        110        120 
KSTFLKQMRI IHGQDFDQRA REEFRPTIYS NVIKGMRVLV DAREKLHIPW GDNKNQLHGD 

       130        140        150        160        170        180 
KLMAFDTRAP MAAQGMVETR VFLQYLPAIR ALWEDSGIQN AYDRRREFQL GESVKYFLDN 

       190        200        210        220        230        240 
LDKLGVPDYI PSQQDILLAR RPTKGIHEYD FEIKNVPFKM VDVGGQRSER KRWFECFDSV 

       250        260        270        280        290        300 
TSILFLVSSS EFDQVLMEDR QTNRLTESLN IFETIVNNRV FSNVSIILFL NKTDLLEEKV 

       310        320        330        340        350        360 
QVVSIKDYFL EFEGDPHCLR DVQKFLVECF RGKRRDQQQR PLYHHFTTAI NTENIRLVFR 

       370 
DVKDTILHDN LKQLMLQ 

« Hide

References

« Hide 'large scale' references
[1]"G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits."
Strathmann M.P., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 88:5582-5586(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: NMRI.
Tissue: Mammary tumor.
[3]Lubec G., Kang S.U.
Submitted (APR-2007) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 51-61 AND 220-227, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Brain.
[4]"Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
Strathmann M., Wilkie T.M., Simon M.I.
Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 228-288.
[5]"RGS22, a novel testis-specific regulator of G-protein signaling involved in human and mouse spermiogenesis along with GNA12/13 subunits."
Hu Y., Xing J., Chen L., Guo X., Du Y., Zhao C., Zhu Y., Lin M., Zhou Z., Sha J.
Biol. Reprod. 79:1021-1029(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
Tissue: Testis.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M63660 mRNA. Translation: AAA37649.1.
BC057665 mRNA. Translation: AAH57665.1.
M57620 mRNA. Translation: AAA63303.1.
CCDSCCDS25577.1.
PIRB41095.
RefSeqNP_034433.3. NM_010303.3.
UniGeneMm.193925.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SHZX-ray2.85A/D52-159[»]
A/D333-376[»]
1ZCBX-ray2.00A47-377[»]
3AB3X-ray2.40A/C47-377[»]
3CX6X-ray2.50A41-377[»]
3CX7X-ray2.25A41-377[»]
3CX8X-ray2.00A41-377[»]
ProteinModelPortalP27601.
SMRP27601. Positions 47-369.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199963. 2 interactions.
DIPDIP-46241N.
IntActP27601. 3 interactions.
MINTMINT-4096246.

PTM databases

PhosphoSiteP27601.

Proteomic databases

MaxQBP27601.
PaxDbP27601.
PRIDEP27601.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000020930; ENSMUSP00000020930; ENSMUSG00000020611.
GeneID14674.
KEGGmmu:14674.
UCSCuc007mce.1. mouse.

Organism-specific databases

CTD10672.
MGIMGI:95768. Gna13.

Phylogenomic databases

eggNOGNOG279688.
GeneTreeENSGT00690000101672.
HOGENOMHOG000038729.
HOVERGENHBG063184.
InParanoidP27601.
KOK04639.
OMACFPGCVL.
OrthoDBEOG7HF1JF.
PhylomeDBP27601.
TreeFamTF300673.

Enzyme and pathway databases

ReactomeREACT_224553. Hemostasis.

Gene expression databases

ArrayExpressP27601.
BgeeP27601.
CleanExMM_GNA13.
GenevestigatorP27601.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR000469. Gprotein_alpha_12.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

ChiTaRSGNA13. mouse.
EvolutionaryTraceP27601.
NextBio286562.
PROP27601.
SOURCESearch...

Entry information

Entry nameGNA13_MOUSE
AccessionPrimary (citable) accession number: P27601
Secondary accession number(s): Q6PF99
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 9, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot