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P27600

- GNA12_MOUSE

UniProt

P27600 - GNA12_MOUSE

Protein

Guanine nucleotide-binding protein subunit alpha-12

Gene

Gna12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. May play a role in the control of cell migration through the TOR signaling cascade.1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi69 – 691MagnesiumBy similarity
    Metal bindingi206 – 2061MagnesiumBy similarity
    Binding sitei351 – 3511GTP; via amide nitrogenBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 698GTPBy similarity
    Nucleotide bindingi200 – 2067GTPBy similarity
    Nucleotide bindingi225 – 2295GTPBy similarity
    Nucleotide bindingi294 – 2974GTPBy similarity

    GO - Molecular functioni

    1. D5 dopamine receptor binding Source: RefGenome
    2. G-protein beta/gamma-subunit complex binding Source: RefGenome
    3. GTPase activity Source: MGI
    4. GTP binding Source: UniProtKB-KW
    5. metal ion binding Source: UniProtKB-KW
    6. signal transducer activity Source: RefGenome

    GO - Biological processi

    1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
    2. cell differentiation Source: MGI
    3. embryonic digit morphogenesis Source: MGI
    4. G-protein coupled receptor signaling pathway Source: MGI
    5. intracellular signal transduction Source: MGI
    6. in utero embryonic development Source: MGI
    7. regulation of cell shape Source: MGI
    8. regulation of fibroblast migration Source: UniProtKB
    9. regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
    10. regulation of TOR signaling Source: UniProtKB
    11. response to drug Source: Ensembl
    12. Rho protein signal transduction Source: MGI

    Keywords - Molecular functioni

    Transducer

    Keywords - Ligandi

    GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_204733. G alpha (12/13) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanine nucleotide-binding protein subunit alpha-12
    Short name:
    G alpha-12
    Short name:
    G-protein subunit alpha-12
    Gene namesi
    Name:Gna12
    Synonyms:Gna-12
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:95767. Gna12.

    Subcellular locationi

    GO - Cellular componenti

    1. brush border membrane Source: RefGenome
    2. heterotrimeric G-protein complex Source: RefGenome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi2 – 21S → G: Results in myristoylation of G-2, which restores the transformation activity; when associated with S-6. 1 Publication
    Mutagenesisi6 – 61R → S: Results in myristoylation of G-2, which restores the transformation activity; when associated with G-2. 1 Publication
    Mutagenesisi11 – 111C → S: Abolishes palmitoylation and transformation activity. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 379379Guanine nucleotide-binding protein subunit alpha-12PRO_0000203771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Lipidationi11 – 111S-palmitoyl cysteine1 Publication

    Post-translational modificationi

    Myristoylation of mutated N-terminus in place of original palmitoylation restores the transformation activity.1 Publication

    Keywords - PTMi

    Lipoprotein, Palmitate

    Proteomic databases

    PaxDbiP27600.
    PRIDEiP27600.

    PTM databases

    PhosphoSiteiP27600.

    Expressioni

    Gene expression databases

    ArrayExpressiP27600.
    BgeeiP27600.
    CleanExiMM_GNA12.
    GenevestigatoriP27600.

    Interactioni

    Subunit structurei

    G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane By similarity. Interacts with RGS22 By similarity.By similarity

    Protein-protein interaction databases

    IntActiP27600. 2 interactions.
    MINTiMINT-4096224.
    STRINGi10090.ENSMUSP00000000153.

    Structurei

    Secondary structure

    1
    379
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi56 – 6712
    Helixi68 – 7912
    Helixi85 – 11228
    Helixi120 – 1223
    Helixi123 – 1308
    Helixi142 – 15716
    Helixi159 – 1668
    Helixi168 – 1703
    Helixi176 – 1827
    Helixi184 – 1874
    Helixi196 – 2016
    Beta strandi208 – 2169
    Beta strandi219 – 2268
    Helixi230 – 2334
    Helixi234 – 2385
    Turni239 – 2424
    Beta strandi244 – 2518
    Beta strandi260 – 2667
    Helixi267 – 27913
    Helixi282 – 2843
    Beta strandi287 – 2948
    Helixi296 – 30510
    Turni309 – 3113
    Helixi322 – 33514
    Beta strandi346 – 3483
    Helixi354 – 37017

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1ZCAX-ray2.90A/B48-379[»]
    ProteinModelPortaliP27600.
    SMRiP27600. Positions 54-371.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP27600.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the G-alpha family. G(12) subfamily.Curated

    Phylogenomic databases

    eggNOGiNOG279688.
    GeneTreeiENSGT00690000101672.
    HOGENOMiHOG000038729.
    HOVERGENiHBG063184.
    InParanoidiP27600.
    KOiK04346.
    OMAiWQHSENE.
    OrthoDBiEOG7HF1JF.
    PhylomeDBiP27600.
    TreeFamiTF300673.

    Family and domain databases

    Gene3Di1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProiIPR000469. Gprotein_alpha_12.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view]
    PANTHERiPTHR10218. PTHR10218. 1 hit.
    PfamiPF00503. G-alpha. 1 hit.
    [Graphical view]
    PRINTSiPR00318. GPROTEINA.
    PR00440. GPROTEINA12.
    SMARTiSM00275. G_alpha. 1 hit.
    [Graphical view]
    SUPFAMiSSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    P27600-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR    50
    AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL 100
    KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP 150
    ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL 200
    LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV 250
    SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV 300
    EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR SKPLFHHFTT 350
    AIDTENIRFV FHAVKDTILQ ENLKDIMLQ 379
    Length:379
    Mass (Da):44,095
    Last modified:January 23, 2007 - v3
    Checksum:i647C524AD7A0B5E2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63659 mRNA. Translation: AAA37648.1.
    M57618 mRNA. Translation: AAA63302.1.
    CCDSiCCDS19825.1.
    PIRiA41095.
    C33833.
    RefSeqiNP_034432.1. NM_010302.2.
    UniGeneiMm.370185.

    Genome annotation databases

    EnsembliENSMUST00000000153; ENSMUSP00000000153; ENSMUSG00000000149.
    GeneIDi14673.
    KEGGimmu:14673.
    UCSCiuc009aih.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M63659 mRNA. Translation: AAA37648.1 .
    M57618 mRNA. Translation: AAA63302.1 .
    CCDSi CCDS19825.1.
    PIRi A41095.
    C33833.
    RefSeqi NP_034432.1. NM_010302.2.
    UniGenei Mm.370185.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1ZCA X-ray 2.90 A/B 48-379 [» ]
    ProteinModelPortali P27600.
    SMRi P27600. Positions 54-371.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P27600. 2 interactions.
    MINTi MINT-4096224.
    STRINGi 10090.ENSMUSP00000000153.

    PTM databases

    PhosphoSitei P27600.

    Proteomic databases

    PaxDbi P27600.
    PRIDEi P27600.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000153 ; ENSMUSP00000000153 ; ENSMUSG00000000149 .
    GeneIDi 14673.
    KEGGi mmu:14673.
    UCSCi uc009aih.1. mouse.

    Organism-specific databases

    CTDi 2768.
    MGIi MGI:95767. Gna12.

    Phylogenomic databases

    eggNOGi NOG279688.
    GeneTreei ENSGT00690000101672.
    HOGENOMi HOG000038729.
    HOVERGENi HBG063184.
    InParanoidi P27600.
    KOi K04346.
    OMAi WQHSENE.
    OrthoDBi EOG7HF1JF.
    PhylomeDBi P27600.
    TreeFami TF300673.

    Enzyme and pathway databases

    Reactomei REACT_204733. G alpha (12/13) signalling events.
    REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

    Miscellaneous databases

    EvolutionaryTracei P27600.
    NextBioi 286558.
    PROi P27600.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27600.
    Bgeei P27600.
    CleanExi MM_GNA12.
    Genevestigatori P27600.

    Family and domain databases

    Gene3Di 1.10.400.10. 1 hit.
    3.40.50.300. 2 hits.
    InterProi IPR000469. Gprotein_alpha_12.
    IPR001019. Gprotein_alpha_su.
    IPR011025. GproteinA_insert.
    IPR027417. P-loop_NTPase.
    [Graphical view ]
    PANTHERi PTHR10218. PTHR10218. 1 hit.
    Pfami PF00503. G-alpha. 1 hit.
    [Graphical view ]
    PRINTSi PR00318. GPROTEINA.
    PR00440. GPROTEINA12.
    SMARTi SM00275. G_alpha. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47895. SSF47895. 1 hit.
    SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits."
      Strathmann M.P., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 88:5582-5586(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Brain.
    2. "Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
      Strathmann M., Wilkie T.M., Simon M.I.
      Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 232-292.
    3. "Galpha12 requires acylation for its transforming activity."
      Jones T.L., Gutkind J.S.
      Biochemistry 37:3196-3202(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: PALMITOYLATION AT CYS-11, MUTAGENESIS OF SER-2; ARG-6 AND CYS-11.
    4. "PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12."
      Gan X., Wang J., Wang C., Sommer E., Kozasa T., Srinivasula S., Alessi D., Offermanns S., Simon M.I., Wu D.
      Nat. Cell Biol. 14:686-696(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION IN TOR SIGNALING.

    Entry informationi

    Entry nameiGNA12_MOUSE
    AccessioniPrimary (citable) accession number: P27600
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 134 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3