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P27600

- GNA12_MOUSE

UniProt

P27600 - GNA12_MOUSE

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Protein

Guanine nucleotide-binding protein subunit alpha-12

Gene

Gna12

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. May play a role in the control of cell migration through the TOR signaling cascade.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691MagnesiumBy similarity
Metal bindingi206 – 2061MagnesiumBy similarity
Binding sitei351 – 3511GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi62 – 698GTPBy similarity
Nucleotide bindingi200 – 2067GTPBy similarity
Nucleotide bindingi225 – 2295GTPBy similarity
Nucleotide bindingi294 – 2974GTPBy similarity

GO - Molecular functioni

  1. D5 dopamine receptor binding Source: RefGenome
  2. G-protein beta/gamma-subunit complex binding Source: RefGenome
  3. GTPase activity Source: MGI
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. signal transducer activity Source: RefGenome

GO - Biological processi

  1. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: RefGenome
  2. cell differentiation Source: MGI
  3. embryonic digit morphogenesis Source: MGI
  4. G-protein coupled receptor signaling pathway Source: MGI
  5. intracellular signal transduction Source: MGI
  6. in utero embryonic development Source: MGI
  7. regulation of cell shape Source: MGI
  8. regulation of fibroblast migration Source: UniProtKB
  9. regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
  10. regulation of TOR signaling Source: UniProtKB
  11. response to drug Source: Ensembl
  12. Rho protein signal transduction Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_204733. G alpha (12/13) signalling events.
REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein subunit alpha-12
Short name:
G alpha-12
Short name:
G-protein subunit alpha-12
Gene namesi
Name:Gna12
Synonyms:Gna-12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 5

Organism-specific databases

MGIiMGI:95767. Gna12.

Subcellular locationi

GO - Cellular componenti

  1. brush border membrane Source: RefGenome
  2. heterotrimeric G-protein complex Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 21S → G: Results in myristoylation of G-2, which restores the transformation activity; when associated with S-6. 1 Publication
Mutagenesisi6 – 61R → S: Results in myristoylation of G-2, which restores the transformation activity; when associated with G-2. 1 Publication
Mutagenesisi11 – 111C → S: Abolishes palmitoylation and transformation activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Guanine nucleotide-binding protein subunit alpha-12PRO_0000203771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi11 – 111S-palmitoyl cysteine1 Publication

Post-translational modificationi

Myristoylation of mutated N-terminus in place of original palmitoylation restores the transformation activity.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate

Proteomic databases

MaxQBiP27600.
PaxDbiP27600.
PRIDEiP27600.

PTM databases

PhosphoSiteiP27600.

Expressioni

Gene expression databases

BgeeiP27600.
CleanExiMM_GNA12.
ExpressionAtlasiP27600. baseline and differential.
GenevestigatoriP27600.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. Interacts with UBXD5. Interacts (when active) with PPP5C (via TPR repeats); activates PPP5C phosphatase activity and translocates PPP5C to the cell membrane By similarity. Interacts with RGS22 By similarity.By similarity

Protein-protein interaction databases

IntActiP27600. 2 interactions.
MINTiMINT-4096224.
STRINGi10090.ENSMUSP00000000153.

Structurei

Secondary structure

1
379
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi56 – 6712
Helixi68 – 7912
Helixi85 – 11228
Helixi120 – 1223
Helixi123 – 1308
Helixi142 – 15716
Helixi159 – 1668
Helixi168 – 1703
Helixi176 – 1827
Helixi184 – 1874
Helixi196 – 2016
Beta strandi208 – 2169
Beta strandi219 – 2268
Helixi230 – 2334
Helixi234 – 2385
Turni239 – 2424
Beta strandi244 – 2518
Beta strandi260 – 2667
Helixi267 – 27913
Helixi282 – 2843
Beta strandi287 – 2948
Helixi296 – 30510
Turni309 – 3113
Helixi322 – 33514
Beta strandi346 – 3483
Helixi354 – 37017

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1ZCAX-ray2.90A/B48-379[»]
ProteinModelPortaliP27600.
SMRiP27600. Positions 54-371.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27600.

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(12) subfamily.Curated

Phylogenomic databases

eggNOGiNOG279688.
GeneTreeiENSGT00760000118851.
HOGENOMiHOG000038729.
HOVERGENiHBG063184.
InParanoidiP27600.
KOiK04346.
OMAiWQHSENE.
OrthoDBiEOG7HF1JF.
PhylomeDBiP27600.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR000469. Gprotein_alpha_12.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

P27600-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGVVRTLSR CLLPAEAGAR ERRAGAARDA EREARRRSRD IDALLARERR
60 70 80 90 100
AVRRLVKILL LGAGESGKST FLKQMRIIHG REFDQKALLE FRDTIFDNIL
110 120 130 140 150
KGSRVLVDAR DKLGIPWQHS ENEKHGMFLM AFENKAGLPV EPATFQLYVP
160 170 180 190 200
ALSALWRDSG IREAFSRRSE FQLGESVKYF LDNLDRIGQL NYFPSKQDIL
210 220 230 240 250
LARKATKGIV EHDFVIKKIP FKMVDVGGQR SQRQKWFQCF DGITSILFMV
260 270 280 290 300
SSSEYDQVLM EDRRTNRLVE SMNIFETIVN NKLFFNVSII LFLNKMDLLV
310 320 330 340 350
EKVKSVSIKK HFPDFKGDPH RLEDVQRYLV QCFDRKRRNR SKPLFHHFTT
360 370
AIDTENIRFV FHAVKDTILQ ENLKDIMLQ
Length:379
Mass (Da):44,095
Last modified:January 23, 2007 - v3
Checksum:i647C524AD7A0B5E2
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63659 mRNA. Translation: AAA37648.1.
M57618 mRNA. Translation: AAA63302.1.
CCDSiCCDS19825.1.
PIRiA41095.
C33833.
RefSeqiNP_034432.1. NM_010302.2.
UniGeneiMm.370185.

Genome annotation databases

EnsembliENSMUST00000000153; ENSMUSP00000000153; ENSMUSG00000000149.
GeneIDi14673.
KEGGimmu:14673.
UCSCiuc009aih.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M63659 mRNA. Translation: AAA37648.1 .
M57618 mRNA. Translation: AAA63302.1 .
CCDSi CCDS19825.1.
PIRi A41095.
C33833.
RefSeqi NP_034432.1. NM_010302.2.
UniGenei Mm.370185.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1ZCA X-ray 2.90 A/B 48-379 [» ]
ProteinModelPortali P27600.
SMRi P27600. Positions 54-371.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P27600. 2 interactions.
MINTi MINT-4096224.
STRINGi 10090.ENSMUSP00000000153.

PTM databases

PhosphoSitei P27600.

Proteomic databases

MaxQBi P27600.
PaxDbi P27600.
PRIDEi P27600.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000153 ; ENSMUSP00000000153 ; ENSMUSG00000000149 .
GeneIDi 14673.
KEGGi mmu:14673.
UCSCi uc009aih.1. mouse.

Organism-specific databases

CTDi 2768.
MGIi MGI:95767. Gna12.

Phylogenomic databases

eggNOGi NOG279688.
GeneTreei ENSGT00760000118851.
HOGENOMi HOG000038729.
HOVERGENi HBG063184.
InParanoidi P27600.
KOi K04346.
OMAi WQHSENE.
OrthoDBi EOG7HF1JF.
PhylomeDBi P27600.
TreeFami TF300673.

Enzyme and pathway databases

Reactomei REACT_204733. G alpha (12/13) signalling events.
REACT_220322. Thrombin signalling through proteinase activated receptors (PARs).

Miscellaneous databases

EvolutionaryTracei P27600.
NextBioi 286558.
PROi P27600.
SOURCEi Search...

Gene expression databases

Bgeei P27600.
CleanExi MM_GNA12.
ExpressionAtlasi P27600. baseline and differential.
Genevestigatori P27600.

Family and domain databases

Gene3Di 1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProi IPR000469. Gprotein_alpha_12.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR10218. PTHR10218. 1 hit.
Pfami PF00503. G-alpha. 1 hit.
[Graphical view ]
PRINTSi PR00318. GPROTEINA.
PR00440. GPROTEINA12.
SMARTi SM00275. G_alpha. 1 hit.
[Graphical view ]
SUPFAMi SSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNeti Search...

Publicationsi

  1. "G alpha 12 and G alpha 13 subunits define a fourth class of G protein alpha subunits."
    Strathmann M.P., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 88:5582-5586(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Diversity of the G-protein family: sequences from five additional alpha subunits in the mouse."
    Strathmann M., Wilkie T.M., Simon M.I.
    Proc. Natl. Acad. Sci. U.S.A. 86:7407-7409(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 232-292.
  3. "Galpha12 requires acylation for its transforming activity."
    Jones T.L., Gutkind J.S.
    Biochemistry 37:3196-3202(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PALMITOYLATION AT CYS-11, MUTAGENESIS OF SER-2; ARG-6 AND CYS-11.
  4. "PRR5L degradation promotes mTORC2-mediated PKC-delta phosphorylation and cell migration downstream of Galpha12."
    Gan X., Wang J., Wang C., Sommer E., Kozasa T., Srinivasula S., Alessi D., Offermanns S., Simon M.I., Wu D.
    Nat. Cell Biol. 14:686-696(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN TOR SIGNALING.

Entry informationi

Entry nameiGNA12_MOUSE
AccessioniPrimary (citable) accession number: P27600
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: October 29, 2014
This is version 135 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3