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Protein

Hexokinase-1

Gene

HK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30ATP 1By similarity1
Binding sitei155Substrate 1By similarity1
Binding sitei209Glucose-6-phosphate 1By similarity1
Binding sitei232Glucose-6-phosphate 1By similarity1
Binding sitei235Substrate 1By similarity1
Binding sitei260Substrate 1By similarity1
Binding sitei449Glucose-6-phosphate 1By similarity1
Binding sitei603Glucose-6-phosphate 2By similarity1
Binding sitei657Glucose-6-phosphate 2By similarity1
Binding sitei680ATP 2By similarity1
Binding sitei680Glucose-6-phosphate 2By similarity1
Binding sitei683Substrate 2By similarity1
Binding sitei708Substrate 2By similarity1
Binding sitei742Substrate 2By similarity1
Binding sitei897Glucose-6-phosphate 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi84 – 89ATP 1Sequence analysis6
Nucleotide bindingi425 – 426ATP 1By similarity2
Nucleotide bindingi532 – 537ATP 2By similarity6
Nucleotide bindingi747 – 748ATP 2By similarity2
Nucleotide bindingi784 – 788ATP 2By similarity5
Nucleotide bindingi863 – 867ATP 2By similarity5

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP27595.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-1 (EC:2.7.1.1)
Alternative name(s):
Brain form hexokinase
Hexokinase type I
Short name:
HK I
Gene namesi
Name:HK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001975841 – 918Hexokinase-1Add BLAST918

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei337PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP27595.

Interactioni

Subunit structurei

Monomer. Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2 (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP27595.
SMRiP27595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini16 – 458Hexokinase 1PROSITE-ProRule annotationAdd BLAST443
Domaini464 – 906Hexokinase 2PROSITE-ProRule annotationAdd BLAST443

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 12HydrophobicAdd BLAST12
Regioni13 – 475RegulatoryBy similarityAdd BLAST463
Regioni73 – 207Hexokinase small subdomain 1PROSITE-ProRule annotationAdd BLAST135
Regioni84 – 88Glucose-6-phosphate 1 bindingBy similarity5
Regioni172 – 173Substrate 1 bindingBy similarity2
Regioni208 – 447Hexokinase large subdomain 1PROSITE-ProRule annotationAdd BLAST240
Regioni208 – 209Substrate 1 bindingBy similarity2
Regioni291 – 294Substrate 1 bindingBy similarity4
Regioni413 – 415Glucose-6-phosphate 1 bindingBy similarity3
Regioni476 – 918CatalyticBy similarityAdd BLAST443
Regioni521 – 655Hexokinase small subdomain 2PROSITE-ProRule annotationAdd BLAST135
Regioni532 – 536Glucose-6-phosphate 2 bindingBy similarity5
Regioni603 – 604Substrate 2 bindingBy similarity2
Regioni620 – 621Substrate 2 bindingBy similarity2
Regioni656 – 895Hexokinase large subdomain 2PROSITE-ProRule annotationAdd BLAST240
Regioni656 – 657Substrate 2 bindingBy similarity2
Regioni861 – 863Glucose-6-phosphate 2 bindingBy similarity3

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule (By similarity).By similarity

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG005020.
InParanoidiP27595.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLLDIM NRFKKEMKNG
60 70 80 90 100
LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE
110 120 130 140 150
QNRPVHMESE VYDTPENIMH GSGSQLFDHV LECLGDFMEK KKIKDKKLPV
160 170 180 190 200
GFTFSFPCRQ SKIDQAILIT WTKRFKARGA EGNYVVKLLD KAIKKRGDYD
210 220 230 240 250
ANIVAVVNDT VGTMIDCGYD DQHCEVGLII GTGTNACYME ELRQIDFGWG
260 270 280 290 300
DDGRMCINTE WGDLGDDGSL EDIRKEFDRE FRRGSLNPGK QRFEKMVSGR
310 320 330 340 350
YMEDVVRLVL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKDKEGLH
360 370 380 390 400
NAKEILTRLG VERSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN
410 420 430 440 450
KSTPRLRTTV RVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGTG
460 470 480 490 500
KGAAMVTAVA YRLAEQHRQI EETLAHFRLS KQTLMEVKKR LRTEMEMGLR
510 520 530 540 550
KETNSNATVN MLPSFLRSIP DGTEDGDFLA LDLGGTNFRV LLVKIRSGKK
560 570 580 590 600
STVEMHNKIY RIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF
610 620 630 640 650
TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA VKRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGNQ
710 720 730 740 750
RQMCINMEWG AFGDNGCSDD IRTDFDKVVD EYSLNSGNQR FENMISGIYL
760 770 780 790 800
GEIVRNILID FTKKGFLFRG QISEPLKTRG IFETKFLSQI ESDRLALLQV
810 820 830 840 850
RAILQQLGLN STCDDSILVK TVCGVVSKRA AQLCGAGMAA VVEKIRENRG
860 870 880 890 900
LDRLNVTVGV DGTLYKLHPQ FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG
910
AALITAVGVR LRGESAIS
Length:918
Mass (Da):103,064
Last modified:August 1, 1992 - v1
Checksum:i1DCFB7F1D06FE2B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65140 mRNA. Translation: AAA51661.1.
PIRiA55277.
UniGeneiBt.500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65140 mRNA. Translation: AAA51661.1.
PIRiA55277.
UniGeneiBt.500.

3D structure databases

ProteinModelPortaliP27595.
SMRiP27595.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP27595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005020.
InParanoidiP27595.

Enzyme and pathway databases

UniPathwayiUPA00242.
SABIO-RKP27595.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK1_BOVIN
AccessioniPrimary (citable) accession number: P27595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.