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Protein

Hexokinase-1

Gene

HK1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + D-hexose = ADP + D-hexose 6-phosphate.

Enzyme regulationi

Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.

Pathwayi: hexose metabolism

This protein is involved in the pathway hexose metabolism, which is part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the pathway hexose metabolism and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei30 – 301ATP 1By similarity
Binding sitei155 – 1551Substrate 1By similarity
Binding sitei209 – 2091Glucose-6-phosphate 1By similarity
Binding sitei232 – 2321Glucose-6-phosphate 1By similarity
Binding sitei235 – 2351Substrate 1By similarity
Binding sitei260 – 2601Substrate 1By similarity
Binding sitei449 – 4491Glucose-6-phosphate 1By similarity
Binding sitei603 – 6031Glucose-6-phosphate 2By similarity
Binding sitei657 – 6571Glucose-6-phosphate 2By similarity
Binding sitei680 – 6801ATP 2By similarity
Binding sitei680 – 6801Glucose-6-phosphate 2By similarity
Binding sitei683 – 6831Substrate 2By similarity
Binding sitei708 – 7081Substrate 2By similarity
Binding sitei742 – 7421Substrate 2By similarity
Binding sitei897 – 8971Glucose-6-phosphate 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi84 – 896ATP 1Sequence analysis
Nucleotide bindingi425 – 4262ATP 1By similarity
Nucleotide bindingi532 – 5376ATP 2By similarity
Nucleotide bindingi747 – 7482ATP 2By similarity
Nucleotide bindingi784 – 7885ATP 2By similarity
Nucleotide bindingi863 – 8675ATP 2By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

SABIO-RKP27595.
UniPathwayiUPA00242.

Names & Taxonomyi

Protein namesi
Recommended name:
Hexokinase-1 (EC:2.7.1.1)
Alternative name(s):
Brain form hexokinase
Hexokinase type I
Short name:
HK I
Gene namesi
Name:HK1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Hexokinase-1PRO_0000197584Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei337 – 3371PhosphoserineBy similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiP27595.

Interactioni

Subunit structurei

Monomer. Interacts with VDAC1. The HK1-VDAC1 complex interacts with ATF2 (By similarity). Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2 (By similarity). Interacts (via N-terminal spermatogenic cell-specific region) with PFKM (via C-terminus) (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP27595.
SMRiP27595. Positions 16-914.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini16 – 458443Hexokinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini464 – 906443Hexokinase 2PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 1212HydrophobicAdd
BLAST
Regioni13 – 475463RegulatoryBy similarityAdd
BLAST
Regioni73 – 207135Hexokinase small subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni84 – 885Glucose-6-phosphate 1 bindingBy similarity
Regioni172 – 1732Substrate 1 bindingBy similarity
Regioni208 – 447240Hexokinase large subdomain 1PROSITE-ProRule annotationAdd
BLAST
Regioni208 – 2092Substrate 1 bindingBy similarity
Regioni291 – 2944Substrate 1 bindingBy similarity
Regioni413 – 4153Glucose-6-phosphate 1 bindingBy similarity
Regioni476 – 918443CatalyticBy similarityAdd
BLAST
Regioni521 – 655135Hexokinase small subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni532 – 5365Glucose-6-phosphate 2 bindingBy similarity
Regioni603 – 6042Substrate 2 bindingBy similarity
Regioni620 – 6212Substrate 2 bindingBy similarity
Regioni656 – 895240Hexokinase large subdomain 2PROSITE-ProRule annotationAdd
BLAST
Regioni656 – 6572Substrate 2 bindingBy similarity
Regioni861 – 8633Glucose-6-phosphate 2 bindingBy similarity

Domaini

The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule (By similarity).By similarity

Sequence similaritiesi

Belongs to the hexokinase family.PROSITE-ProRule annotationCurated
Contains 2 hexokinase domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOVERGENiHBG005020.
InParanoidiP27595.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27595-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLLDIM NRFKKEMKNG
60 70 80 90 100
LSRDFNPTAT VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE
110 120 130 140 150
QNRPVHMESE VYDTPENIMH GSGSQLFDHV LECLGDFMEK KKIKDKKLPV
160 170 180 190 200
GFTFSFPCRQ SKIDQAILIT WTKRFKARGA EGNYVVKLLD KAIKKRGDYD
210 220 230 240 250
ANIVAVVNDT VGTMIDCGYD DQHCEVGLII GTGTNACYME ELRQIDFGWG
260 270 280 290 300
DDGRMCINTE WGDLGDDGSL EDIRKEFDRE FRRGSLNPGK QRFEKMVSGR
310 320 330 340 350
YMEDVVRLVL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKDKEGLH
360 370 380 390 400
NAKEILTRLG VERSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN
410 420 430 440 450
KSTPRLRTTV RVDGSLYKTH PQYSRRFHKT LRRLVPDSDV RFLLSESGTG
460 470 480 490 500
KGAAMVTAVA YRLAEQHRQI EETLAHFRLS KQTLMEVKKR LRTEMEMGLR
510 520 530 540 550
KETNSNATVN MLPSFLRSIP DGTEDGDFLA LDLGGTNFRV LLVKIRSGKK
560 570 580 590 600
STVEMHNKIY RIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF
610 620 630 640 650
TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA VKRREEFDLD
660 670 680 690 700
VVAVVNDTVG TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGNQ
710 720 730 740 750
RQMCINMEWG AFGDNGCSDD IRTDFDKVVD EYSLNSGNQR FENMISGIYL
760 770 780 790 800
GEIVRNILID FTKKGFLFRG QISEPLKTRG IFETKFLSQI ESDRLALLQV
810 820 830 840 850
RAILQQLGLN STCDDSILVK TVCGVVSKRA AQLCGAGMAA VVEKIRENRG
860 870 880 890 900
LDRLNVTVGV DGTLYKLHPQ FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG
910
AALITAVGVR LRGESAIS
Length:918
Mass (Da):103,064
Last modified:August 1, 1992 - v1
Checksum:i1DCFB7F1D06FE2B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65140 mRNA. Translation: AAA51661.1.
PIRiA55277.
UniGeneiBt.500.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65140 mRNA. Translation: AAA51661.1.
PIRiA55277.
UniGeneiBt.500.

3D structure databases

ProteinModelPortaliP27595.
SMRiP27595. Positions 16-914.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP27595.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG005020.
InParanoidiP27595.

Enzyme and pathway databases

UniPathwayiUPA00242.
SABIO-RKP27595.

Family and domain databases

InterProiIPR001312. Hexokinase.
IPR019807. Hexokinase_BS.
IPR022673. Hexokinase_C.
IPR022672. Hexokinase_N.
[Graphical view]
PANTHERiPTHR19443. PTHR19443. 1 hit.
PfamiPF00349. Hexokinase_1. 2 hits.
PF03727. Hexokinase_2. 2 hits.
[Graphical view]
PROSITEiPS00378. HEXOKINASE_1. 2 hits.
PS51748. HEXOKINASE_2. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHXK1_BOVIN
AccessioniPrimary (citable) accession number: P27595
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: July 6, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.