Reviewed, UniProtKB/Swiss-Prot P27595 (HXK1_BOVIN)

Names and origin

Protein names

Recommended name:
    Hexokinase-1
    EC=2.7.1.1
Alternative name(s):
    Hexokinase type I
      Short name=HK I
    Brain form hexokinase

Gene names

Name:

HK1

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	918 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + D-hexose = ADP + D-hexose 6-phosphate.
Enzyme regulation	Hexokinase is an allosteric enzyme inhibited by its product Glc-6-P.
Pathway	Carbohydrate metabolism; hexose metabolism.
Subunit structure	Monomer.
Subcellular location	Mitochondrion outer membrane. Note: Its hydrophobic N-terminal sequence may be involved in membrane binding.
Domain	The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule By similarity.
Miscellaneous	In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).
Sequence similarities	Belongs to the hexokinase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Membrane Mitochondrion Mitochondrion outer membrane
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Acetylation Phosphoprotein
Technical term	Allosteric enzyme
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytosol Inferred from sequence or structural similarity. Source: AgBase mitochondrial outer membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hexokinase activity Inferred from sequence or structural similarity. Source: AgBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 918	918	Hexokinase-1		PRO_0000197584
Regions
	Nucleotide binding	84 – 89	6	ATP 1 Potential
	Nucleotide binding	425 – 426	2	ATP 1 By similarity
	Nucleotide binding	532 – 537	6	ATP 2 By similarity
	Nucleotide binding	747 – 748	2	ATP 2 By similarity
	Nucleotide binding	784 – 788	5	ATP 2 By similarity
	Nucleotide binding	863 – 867	5	ATP 2 By similarity
	Region	1 – 12	12	Hydrophobic
	Region	13 – 475	463	Regulatory By similarity
	Region	84 – 88	5	Glucose-6-phosphate 1 binding By similarity
	Region	172 – 173	2	Substrate 1 binding By similarity
	Region	208 – 209	2	Substrate 1 binding By similarity
	Region	291 – 294	4	Substrate 1 binding By similarity
	Region	413 – 415	3	Glucose-6-phosphate 1 binding By similarity
	Region	476 – 918	443	Catalytic By similarity
	Region	532 – 536	5	Glucose-6-phosphate 2 binding By similarity
	Region	603 – 604	2	Substrate 2 binding By similarity
	Region	620 – 621	2	Substrate 2 binding By similarity
	Region	656 – 657	2	Substrate 2 binding By similarity
	Region	861 – 863	3	Glucose-6-phosphate 2 binding By similarity
Sites
	Binding site	30	1	ATP 1 By similarity
	Binding site	155	1	Substrate 1 binding By similarity
	Binding site	209	1	Glucose-6-phosphate 1 By similarity
	Binding site	232	1	Glucose-6-phosphate 1 By similarity
	Binding site	235	1	Substrate 1 By similarity
	Binding site	260	1	Substrate 1 By similarity
	Binding site	449	1	Glucose-6-phosphate 1 By similarity
	Binding site	603	1	Glucose-6-phosphate 2 By similarity
	Binding site	657	1	Glucose-6-phosphate 2 By similarity
	Binding site	680	1	ATP 2 By similarity
	Binding site	680	1	Glucose-6-phosphate 2 By similarity
	Binding site	683	1	Substrate 2 binding By similarity
	Binding site	708	1	Substrate 2 By similarity
	Binding site	742	1	Substrate 2 By similarity
	Binding site	897	1	Glucose-6-phosphate 2 By similarity
Amino acid modifications
	Modified residue	1	1	N-acetylmethionine By similarity
	Modified residue	27	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27595-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 1DCFB7F1D06FE2B6	FASTA	918	103,064
	10 20 30 40 50 60 MIAAQLLAYY FTELKDDQVK KIDKYLYAMR LSDETLLDIM NRFKKEMKNG LSRDFNPTAT 70 80 90 100 110 120 VKMLPTFVRS IPDGSEKGDF IALDLGGSSF RILRVQVNHE QNRPVHMESE VYDTPENIMH 130 140 150 160 170 180 GSGSQLFDHV LECLGDFMEK KKIKDKKLPV GFTFSFPCRQ SKIDQAILIT WTKRFKARGA 190 200 210 220 230 240 EGNYVVKLLD KAIKKRGDYD ANIVAVVNDT VGTMIDCGYD DQHCEVGLII GTGTNACYME 250 260 270 280 290 300 ELRQIDFGWG DDGRMCINTE WGDLGDDGSL EDIRKEFDRE FRRGSLNPGK QRFEKMVSGR 310 320 330 340 350 360 YMEDVVRLVL VKMAKEGLLF EGRITPELLT RGKFNTSDVS AIEKDKEGLH NAKEILTRLG 370 380 390 400 410 420 VERSDDDCVS VQHVCTIVSF RSANLVAATL GAILNRLRDN KSTPRLRTTV RVDGSLYKTH 430 440 450 460 470 480 PQYSRRFHKT LRRLVPDSDV RFLLSESGTG KGAAMVTAVA YRLAEQHRQI EETLAHFRLS 490 500 510 520 530 540 KQTLMEVKKR LRTEMEMGLR KETNSNATVN MLPSFLRSIP DGTEDGDFLA LDLGGTNFRV 550 560 570 580 590 600 LLVKIRSGKK STVEMHNKIY RIPIEIMQGT GEELFDHIVS CISDFLDYMG IKGPRMPLGF 610 620 630 640 650 660 TFSFPCQQTS LDAGILITWT KGFKATDCVG HDVVTLLRDA VKRREEFDLD VVAVVNDTVG 670 680 690 700 710 720 TMMTCAYEEP TCEVGLIVGT GSNACYMEEM KNVEMVEGNQ RQMCINMEWG AFGDNGCSDD 730 740 750 760 770 780 IRTDFDKVVD EYSLNSGNQR FENMISGIYL GEIVRNILID FTKKGFLFRG QISEPLKTRG 790 800 810 820 830 840 IFETKFLSQI ESDRLALLQV RAILQQLGLN STCDDSILVK TVCGVVSKRA AQLCGAGMAA 850 860 870 880 890 900 VVEKIRENRG LDRLNVTVGV DGTLYKLHPQ FSRIMHQTVK ELSPKCNVSF LLSEDGSGKG 910 AALITAVGVR LRGESAIS

References

[1]	"Synthesis and characterization of a bovine hexokinase 1 cDNA probe by mixed oligonucleotide primed amplification of cDNA using high complexity primer mixtures." Griffin L.D., Macgregor G.R., Muzny D.M., Harter J., Cook R.G., McCabe E.R. Biochem. Med. Metab. Biol. 41:125-131(1989) [PubMed: 2719857] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Mammalian hexokinase 1: evolutionary conservation and structure to function analysis." Griffin L.D., Gelb B.D., Wheeler D.A., Davison D., Adams V., McCabe E.R. Genomics 11:1014-1024(1991) [PubMed: 1783373] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M65140 mRNA. Translation: AAA51661.1.
IPI	IPI00705057.
PIR	A55277.
RefSeq	NP_776744.1.
UniGene	Bt.500
3D structure databases
HSSP	HSSP built from PDB template 1QHA based on UniProtKB P19367.
SMR	P27595. Positions 16-912.
ModBase	Search...
Protein-protein interaction databases
STRING	P27595.
Genome annotation databases
GeneID	281771.
KEGG	bta:281771.
Organism-specific databases
CTD	281771.
Phylogenomic databases
HOVERGEN	P27595.
Enzyme and pathway databases
BRENDA	2.7.1.1. 251.
Family and domain databases
InterPro	IPR001312. Hexokinase. IPR019807. Hexokinase_CS. [Graphical view]
PANTHER	PTHR19443. Hexokinase. 1 hit.
Pfam	PF00349. Hexokinase_1. 2 hits. PF03727. Hexokinase_2. 2 hits. [Graphical view]
PRINTS	PR00475. HEXOKINASE.
ProDom	PD001109. Hexokinase. 2 hits. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00378. HEXOKINASES. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HXK1_BOVIN

Accession

Primary (citable) accession number: P27595

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	September 1, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)