P27582 (FABG6_BRANA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: 3-oxoacyl-[acyl-carrier-protein] reductase EC=1.1.1.100 Alternative name(s): 3-ketoacyl-acyl carrier protein reductase
Organism	Brassica napus (Rape)
Taxonomic identifier	3708 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Brassiceae › Brassica

Protein attributes

Sequence length	201 AA.
Sequence status	Fragments.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP+ = 3-oxoacyl-[acyl-carrier-protein] + NADPH.
Pathway	Lipid metabolism; fatty acid biosynthesis.
Subunit structure	Homotetramer Probable.
Subcellular location	Plastid › chloroplast. Note: And non-photosynthetic plastids.
Tissue specificity	Embryo and leaf tissues.
Miscellaneous	Exhibits a marked preference for acyl-carrier protein derivatives over CoA derivatives as substrates.
Sequence similarities	Belongs to the short-chain dehydrogenases/reductases (SDR) family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Chloroplast Plastid
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-oxoacyl-[acyl-carrier-protein] reductase (NADPH) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 201	201	3-oxoacyl-[acyl-carrier-protein] reductase		PRO_0000054661
Sites
	Active site	108	1	Proton acceptor By similarity
	Binding site	95	1	Substrate By similarity
Experimental info
	Non-adjacent residues	27 – 28	2
	Non-adjacent residues	34 – 35	2
	Non-adjacent residues	62 – 63	2

Sequences

	Sequence		Length	Mass (Da)
	P27582 [UniParc]. Last modified February 1, 1994. Version 3. Checksum: FD51B2E369D2D967	FASTA	201	21,047
	10 20 30 40 50 60 ATAQEQPEGE APDKVESPVV XXXEAXIVLV NYAREADVDA MMKDAVDYWG QIDVIANNAG 70 80 90 100 110 120 ITVIALNLTG VFLCSQAATK IMMKKRKGRI INIASVVGLI GNIGQANYAA AKAGVIGFSK 130 140 150 160 170 180 TAAREGASRN INVNVVCPGF IASEMTAKLG EDMEKKILGT IPLGRYGQPE DVAGLVEFLA 190 200 LSPAASYITG QTFTIDGGIA I

References

[1]

"3-oxoacyl-[ACP] reductase from oilseed rape (Brassica napus)." Sheldon P.S., Kekwick R.G.O., Smith C.G., Sidebottom C.M., Slabas A.R. Biochim. Biophys. Acta 1120:151-159(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 35-62 AND 81-84. Tissue: Seed.

[2]

"Molecular cloning of higher-plant 3-oxoacyl-(acyl carrier protein) reductase. Sequence identities with the nodG-gene product of the nitrogen-fixing soil bacterium Rhizobium meliloti." Slabas A.R., Chase D., Nishida I., Murata N., Sidebottom C.M., Safford R., Sheldon P.S., Kekwick R.G.O., Hardie D.G., Mackintosh R.W. Biochem. J. 283:321-326(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 63-201, PARTIAL PROTEIN SEQUENCE. Tissue: Seed.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64463 mRNA. Translation: CAA45793.1.
PIR	S22417.
3D structure databases
ProteinModelPortal	P27582.
SMR	P27582. Positions 31-201.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00094.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR002198. DH_sc/Rdtase_SDR. IPR002347. Glc/ribitol_DH. IPR016040. NAD(P)-bd_dom. IPR020904. Sc_DH/Rdtase_CS. [Graphical view]
Pfam	PF00106. adh_short. 1 hit. [Graphical view]
PRINTS	PR00081. GDHRDH. PR00080. SDRFAMILY.
PROSITE	PS00061. ADH_SHORT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FABG6_BRANA

Accession

Primary (citable) accession number: P27582

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	February 1, 1994
Last modified:	April 3, 2013
This is version 89 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program