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Protein

Protein C-ets-1

Gene

Ets1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcription factor. Directly controls the expression of cytokine and chemokine genes in a wide variety of different cellular contexts. May control the differentiation, survival and proliferation of lymphoid cells. May also regulate angiogenesis through regulation of expression of genes controlling endothelial cell migration and invasion (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi335 – 415ETSPROSITE-ProRule annotationAdd BLAST81

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Immunity, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiR-MMU-2559585. Oncogene Induced Senescence.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein C-ets-1
Alternative name(s):
p54
Gene namesi
Name:Ets1
Synonyms:Ets-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:95455. Ets1.

Subcellular locationi

  • Cytoplasm By similarity
  • Nucleus By similarity

  • Note: Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • nucleoplasm Source: MGI
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002040701 – 440Protein C-ets-1Add BLAST440

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8N6-acetyllysineBy similarity1
Modified residuei15N6-acetyllysine; alternateBy similarity1
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO); alternate
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei38Phosphothreonine; by MAPK1 Publication1
Modified residuei223PhosphotyrosineBy similarity1
Cross-linki227Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO)1 Publication
Modified residuei251PhosphoserineCombined sources1
Modified residuei254PhosphoserineCombined sources1
Modified residuei265PhosphothreonineBy similarity1
Modified residuei267PhosphoserineBy similarity1
Modified residuei270PhosphoserineBy similarity1
Modified residuei282PhosphoserineCombined sources1
Modified residuei285PhosphoserineCombined sources1
Modified residuei305N6-acetyllysineBy similarity1

Post-translational modificationi

Sumoylated on Lys-15 and Lys-227, preferentially with SUMO2; which inhibits transcriptional activity.1 Publication
Ubiquitinated; which induces proteasomal degradation.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP27577.
PaxDbiP27577.
PRIDEiP27577.

PTM databases

iPTMnetiP27577.
PhosphoSitePlusiP27577.

Expressioni

Gene expression databases

BgeeiENSMUSG00000032035.
CleanExiMM_ETS1.
ExpressionAtlasiP27577. baseline and differential.
GenevisibleiP27577. MM.

Interactioni

Subunit structurei

Binds DNA as a homodimer; homodimerization is required for transcription activation. Interacts with DAXX (By similarity). Interacts with UBE2I (By similarity). Interacts with SP100; the interaction is direct and modulates ETS1 transcriptional activity (By similarity). Interacts with MAF and MAFB. Interacts with PAX5; the interaction alters DNA-binding properties.By similarity3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
TLX1P313142EBI-4289053,EBI-2820655From a different organism.
TLX3O437112EBI-4289053,EBI-3939165From a different organism.

Protein-protein interaction databases

BioGridi204765. 4 interactors.
DIPiDIP-41848N.
IntActiP27577. 3 interactors.
MINTiMINT-194860.
STRINGi10090.ENSMUSP00000034534.

Structurei

Secondary structure

1440
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni39 – 42Combined sources4
Helixi43 – 52Combined sources10
Helixi54 – 62Combined sources9
Beta strandi67 – 69Combined sources3
Helixi74 – 88Combined sources15
Turni95 – 98Combined sources4
Helixi102 – 107Combined sources6
Helixi109 – 116Combined sources8
Helixi119 – 134Combined sources16
Helixi304 – 312Combined sources9
Turni313 – 315Combined sources3
Beta strandi318 – 321Combined sources4
Helixi323 – 330Combined sources8
Helixi337 – 345Combined sources9
Helixi348 – 350Combined sources3
Turni351 – 353Combined sources3
Beta strandi354 – 356Combined sources3
Beta strandi362 – 364Combined sources3
Helixi368 – 379Combined sources12
Helixi386 – 395Combined sources10
Turni396 – 400Combined sources5
Beta strandi401 – 404Combined sources4
Beta strandi408 – 414Combined sources7
Helixi418 – 422Combined sources5
Helixi426 – 432Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K78X-ray2.25B/F331-440[»]
1K79X-ray2.40A/D331-440[»]
1K7AX-ray2.80A/D331-440[»]
1MD0X-ray2.00A/B300-440[»]
1MDMX-ray2.80B280-440[»]
1R36NMR-A301-440[»]
2JV3NMR-A29-138[»]
2KMDNMR-A29-138[»]
DisProtiDP00111.
ProteinModelPortaliP27577.
SMRiP27577.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27577.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini51 – 136PNTPROSITE-ProRule annotationAdd BLAST86

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni130 – 243Activation domain; required for transcription activationBy similarityAdd BLAST114

Sequence similaritiesi

Belongs to the ETS family.Curated
Contains 1 ETS DNA-binding domain.PROSITE-ProRule annotation
Contains 1 PNT (pointed) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000285953.
HOVERGENiHBG003088.
InParanoidiP27577.
KOiK02678.
OMAiDEMATQE.
OrthoDBiEOG091G0HJF.
PhylomeDBiP27577.
TreeFamiTF316214.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016311. Transform_prot_C-ets.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFiPIRSF001698. Transforming_factor_C-ets. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: At least 2 isoforms are produced.
Isoform 1 (identifier: P27577-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKAAVDLKPT LTIIKTEKVD LELFPSPDME CADVPLLTPS SKEMMSQALK
60 70 80 90 100
ATFSGFTKEQ QRLGIPKDPR QWTETHVRDW VMWAVNEFSL KGVDFQKFCM
110 120 130 140 150
SGAALCALGK ECFLELAPDF VGDILWEHLE ILQKEDVKPY QVNGANPTYP
160 170 180 190 200
ESCYTSDYFI SYGIEHAQCV PPSEFSEPSF ITESYQTLHP ISSEELLSLK
210 220 230 240 250
YENDYPSVIL QDPLQTDTLQ TDYFAIKQEV LTPDNMCLGR ASRGKLGGQD
260 270 280 290 300
SFESVESYDS CDRLTQSWSS QSSFNSLQRV PSYDSFDYED YPAALPNHKP
310 320 330 340 350
KGTFKDYVRD RADLNKDKPV IPAAALAGYT GSGPIQLWQF LLELLTDKSC
360 370 380 390 400
QSFISWTGDG WEFKLSDPDE VARRWGKRKN KPKMNYEKLS RGLRYYYDKN
410 420 430 440
IIHKTAGKRY VYRFVCDLQS LLGYTPEELH AMLDVKPDAD
Length:440
Mass (Da):50,202
Last modified:February 1, 1995 - v2
Checksum:i151164D83C41B143
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti28D → E in AAA63299 (Ref. 1) Curated1
Sequence conflicti28D → E in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti37L → S in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti51 – 52AT → SY in CAA39310 (PubMed:2204020).Curated2
Sequence conflicti55G → P in AAA63299 (Ref. 1) Curated1
Sequence conflicti63L → R in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti74E → D in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti96Q → H in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti105L → V in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti157D → V in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti211Q → R in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti217D → E in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti225A → R in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti234D → N in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti360G → C in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti383K → S in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti392G → A in CAA39310 (PubMed:2204020).Curated1
Sequence conflicti408 – 409KR → NA in CAA39310 (PubMed:2204020).Curated2
Sequence conflicti413R → A in CAA39310 (PubMed:2204020).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58482 mRNA. Translation: AAA63299.1.
X53953 mRNA. Translation: CAA37904.1.
X55787 mRNA. Translation: CAA39310.1.
BC010588 mRNA. Translation: AAH10588.1.
CCDSiCCDS22954.1. [P27577-1]
PIRiA30487. A35875.
I48291.
RefSeqiNP_035938.2. NM_011808.2. [P27577-1]
UniGeneiMm.292415.

Genome annotation databases

EnsembliENSMUST00000034534; ENSMUSP00000034534; ENSMUSG00000032035. [P27577-1]
GeneIDi23871.
KEGGimmu:23871.
UCSCiuc009osb.1. mouse. [P27577-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58482 mRNA. Translation: AAA63299.1.
X53953 mRNA. Translation: CAA37904.1.
X55787 mRNA. Translation: CAA39310.1.
BC010588 mRNA. Translation: AAH10588.1.
CCDSiCCDS22954.1. [P27577-1]
PIRiA30487. A35875.
I48291.
RefSeqiNP_035938.2. NM_011808.2. [P27577-1]
UniGeneiMm.292415.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1K78X-ray2.25B/F331-440[»]
1K79X-ray2.40A/D331-440[»]
1K7AX-ray2.80A/D331-440[»]
1MD0X-ray2.00A/B300-440[»]
1MDMX-ray2.80B280-440[»]
1R36NMR-A301-440[»]
2JV3NMR-A29-138[»]
2KMDNMR-A29-138[»]
DisProtiDP00111.
ProteinModelPortaliP27577.
SMRiP27577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi204765. 4 interactors.
DIPiDIP-41848N.
IntActiP27577. 3 interactors.
MINTiMINT-194860.
STRINGi10090.ENSMUSP00000034534.

PTM databases

iPTMnetiP27577.
PhosphoSitePlusiP27577.

Proteomic databases

EPDiP27577.
PaxDbiP27577.
PRIDEiP27577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034534; ENSMUSP00000034534; ENSMUSG00000032035. [P27577-1]
GeneIDi23871.
KEGGimmu:23871.
UCSCiuc009osb.1. mouse. [P27577-1]

Organism-specific databases

CTDi2113.
MGIiMGI:95455. Ets1.

Phylogenomic databases

eggNOGiKOG3806. Eukaryota.
ENOG410Z0ZF. LUCA.
GeneTreeiENSGT00760000118907.
HOGENOMiHOG000285953.
HOVERGENiHBG003088.
InParanoidiP27577.
KOiK02678.
OMAiDEMATQE.
OrthoDBiEOG091G0HJF.
PhylomeDBiP27577.
TreeFamiTF316214.

Enzyme and pathway databases

ReactomeiR-MMU-2559585. Oncogene Induced Senescence.

Miscellaneous databases

ChiTaRSiEts1. mouse.
EvolutionaryTraceiP27577.
PROiP27577.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032035.
CleanExiMM_ETS1.
ExpressionAtlasiP27577. baseline and differential.
GenevisibleiP27577. MM.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.150.50. 1 hit.
InterProiIPR000418. Ets_dom.
IPR003118. Pointed_dom.
IPR013761. SAM/pointed.
IPR016311. Transform_prot_C-ets.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF00178. Ets. 1 hit.
PF02198. SAM_PNT. 1 hit.
[Graphical view]
PIRSFiPIRSF001698. Transforming_factor_C-ets. 1 hit.
PRINTSiPR00454. ETSDOMAIN.
SMARTiSM00413. ETS. 1 hit.
SM00251. SAM_PNT. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF47769. SSF47769. 1 hit.
PROSITEiPS00345. ETS_DOMAIN_1. 1 hit.
PS00346. ETS_DOMAIN_2. 1 hit.
PS50061. ETS_DOMAIN_3. 1 hit.
PS51433. PNT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiETS1_MOUSE
AccessioniPrimary (citable) accession number: P27577
Secondary accession number(s): Q61403
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1995
Last modified: November 30, 2016
This is version 173 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.