Reviewed, UniProtKB/Swiss-Prot P27561 (RIP4_SAPOF)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein saporin-4
      Short name=SAP-4
    EC=3.2.2.22
Alternative name(s):
    SO-3
    rRNA N-glycosidase

Gene names

Name:

SAP4

Organism

Saponaria officinalis (Common soapwort)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	157 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.
Sequence caution	The sequence CAA41950.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – ›157	›133	Ribosome-inactivating protein saporin-4		PRO_0000030775
Experimental info
	Non-terminal residue	157	1

Sequences

	Sequence		Length	Mass (Da)
	P27561-1 [UniParc]. Last modified April 1, 1993. Version 2. Checksum: 22DB3FFFDE18288C	FASTA	157	17,520
	10 20 30 40 50 60 MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN VKDPNLKYGG 70 80 90 100 110 120 TDIAVIGPPS KGKFLRINFQ SSRGTVSLGL KRDNLYVVAY LAMDNTNVNR AYYFRSEITS 130 140 150 AELTALFPEA TTANQKALEY TEDYQSIEKN AQITQED

References

[1]	"Characterisation of saporin genes: in vitro expression and ribosome inactivation." Fordham-Skelton A.P., Taylor P.E., Hartley M.R., Croy R.R.D. Mol. Gen. Genet. 229:460-466(1991) [PubMed: 1719367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59257 Genomic DNA. Translation: CAA41950.1. Sequence problems.
PIR	S16485. S16487. S17934. S38527.
3D structure databases
HSSP	HSSP built from PDB template 1QI7 based on UniProtKB P27559.
SMR	P27561. Positions 25-157.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 39707.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP4_SAPOF

Accession

Primary (citable) accession number: P27561

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	April 1, 1993
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)