P27560 (RIP3_SAPOF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-inactivating protein saporin-3
Short name=SAP-3
Short name=SO-3
EC=3.2.2.22

Alternative name(s):
rRNA N-glycosidase

Gene names

Name:

SAP3

Organism

Saponaria officinalis (Common soapwort) (Lychnis saponaria)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	236 AA.
Sequence status	Fragment.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 236	›236	Ribosome-inactivating protein saporin-3		PRO_0000221412
Sites
	Active site	148	1	By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P27560 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: DE3D68BA50FCE054	FASTA	236	26,591
	10 20 30 40 50 60 DPNLKYGGTD IAVIGPPSRD KFLRLNFQSS RGTVSLGLKR ENLYVVAYLA MDNANVNRAY 70 80 90 100 110 120 YFGTEITSAE LTTLLPEATV ANQKALEYTE DYQSIEKNAK ITEGDKTRKE LGLGINLLST 130 140 150 160 170 180 LMDAVNKKAR VVKNEARFLL IAIQMTAEAA RFRYIQNLVT KNFPNKFNSE DKVIQFQVNW 190 200 210 220 230 SKISKAIYGD AKNGVFNKDY DFGFGKVRQV KDLQMGLLMY LGTTPNNAAD RYRAEL

References

[1]	"Characterisation of saporin genes: in vitro expression and ribosome inactivation." Fordham-Skelton A.P., Taylor P.E., Hartley M.R., Croy R.R.D. Mol. Gen. Genet. 229:460-466(1991) [PubMed: 1719367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X59256 Genomic DNA. Translation: CAA41949.1.
PIR	S17932.
3D structure databases
ProteinModelPortal	P27560.
SMR	P27560. Positions 1-222.
ModBase	Search...
Protein family/group databases
Allergome	2805. Sap o RIP.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
SUPFAM	SSF56371. Ribosome_inactivat_prot. 1 hit.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP3_SAPOF

Accession

Primary (citable) accession number: P27560

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 28, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program