Reviewed, UniProtKB/Swiss-Prot P27559 (RIP2_SAPOF)

Names and origin

Protein names

Recommended name:
    Ribosome-inactivating protein saporin-2
      Short name=SAP-2
      Short name=SO-2
    EC=3.2.2.22
Alternative name(s):
    rRNA N-glycosidase

Gene names

Name:

SAP2

Organism

Saponaria officinalis (Common soapwort)

Taxonomic identifier

3572 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › Caryophyllales › Caryophyllaceae › Saponaria

Protein attributes

Sequence length	292 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.
Catalytic activity	Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
Sequence similarities	Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Hydrolase Protein synthesis inhibitor Toxin
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	defense response Inferred from electronic annotation. Source: UniProtKB-KW negative regulation of translation Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	rRNA N-glycosylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Ref.3
	Chain	25 – 292	268	Ribosome-inactivating protein saporin-2		PRO_0000030774
Sites
	Active site	200	1	By similarity
Natural variations
	Natural variant	72	1	D → E

Sequences

	Sequence		Length	Mass (Da)
	P27559-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: FA143C0E1BE88976	FASTA	292	32,811
	10 20 30 40 50 60 MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN VKDPNLKYGG 70 80 90 100 110 120 TDIAVIGPPS KDKFLRINFQ SSRGTVSLGL KRDNLYVVAY LAMDNTNVNR AYYFKSEITS 130 140 150 160 170 180 AELTALFPEA TTANQKALEY TEDYQSIEKN AQITQGDKSR KELGLGIDLL LTFMEAVNKK 190 200 210 220 230 240 ARVVKNEARF LLIAIQMTAE VARFRYIQNL VTKNFPNKFD SDNKVIQFEV SWRKISTAIY 250 260 270 280 290 GDAKNGVFNK DYDFGFGKVR QVKDLQMGLL MYLGKPKSSN EANSTAYATT VL

References

[1]	"Characterisation of saporin genes: in vitro expression and ribosome inactivation." Fordham-Skelton A.P., Taylor P.E., Hartley M.R., Croy R.R.D. Mol. Gen. Genet. 229:460-466(1991) [PubMed: 1719367] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The expression of saporin, a ribosome-inactivating protein from the plant Saponaria officinalis, in Escherichia coli." Barthelemy I., Martineau D., Ong M., Matsunami R., Ling N., Benatti L., Cavallaro U., Soria M., Lappi D.A. J. Biol. Chem. 268:6541-6548(1993) [PubMed: 8454624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-284. Tissue: Leaf.
[3]	"Distribution and properties of major ribosome-inactivating proteins (28 S rRNA N-glycosidases) of the plant Saponaria officinalis L. (Caryophyllaceae)." Ferreras J.M., Barbieri L., Girbes T., Battelli M.G., Rojo M.A., Arias F.J., Rocher M.A., Soriano F., Mendez E., Stirpe F. Biochim. Biophys. Acta 1216:31-42(1993) [PubMed: 8218413] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-54.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X59255 Genomic DNA. Translation: CAA41948.1. X69132 Genomic DNA. Translation: CAA48886.1. X69133 Genomic DNA. Translation: CAA48887.1.
PIR	S16487. RLQHG2. S17933. S38527.
3D structure databases
HSSP	HSSP built from PDB template 1QI7 based on UniProtKB P20656.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.2.2.22. 39707.
Family and domain databases
InterPro	IPR001574. Ribosome_inactivat_prot. IPR017988. Ribosome_inactivat_prot_CS. IPR016138. Ribosome_inactivat_prot_sub1. IPR016139. Ribosome_inactivat_prot_sub2. IPR017989. Ribosome_inactivat_prot_subgr. [Graphical view]
Gene3D	G3DSA:3.40.420.10. Ribosome_inactivat_prot_sub1. 1 hit. G3DSA:4.10.470.10. Ribosome_inactivat_prot_sub2. 1 hit.
Pfam	PF00161. RIP. 1 hit. [Graphical view]
PRINTS	PR00396. SHIGARICIN.
PROSITE	PS00275. SHIGA_RICIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIP2_SAPOF

Accession

Primary (citable) accession number: P27559
Secondary accession number(s): Q41390, Q9SAP5

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)