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Protein

Ribosome-inactivating protein saporin-2

Gene

SAP2

Organism
Saponaria officinalis (Common soapwort) (Lychnis saponaria)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells. Useful as immunotoxin for pharmacological applications.

Catalytic activityi

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei200 – 2001By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protein synthesis inhibitor, Toxin

Keywords - Biological processi

Plant defense

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-inactivating protein saporin-2 (EC:3.2.2.22)
Short name:
SAP-2
Short name:
SO-2
Alternative name(s):
rRNA N-glycosidase
Gene namesi
Name:SAP2
OrganismiSaponaria officinalis (Common soapwort) (Lychnis saponaria)
Taxonomic identifieri3572 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesCaryophyllaceaeCaryophylleaeSaponaria

Pathology & Biotechi

Protein family/group databases

Allergomei2805. Sap o RIP.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 24241 PublicationAdd
BLAST
Chaini25 – 292268Ribosome-inactivating protein saporin-2PRO_0000030774Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP27559.
SMRiP27559. Positions 25-277.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27559-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN
60 70 80 90 100
VKDPNLKYGG TDIAVIGPPS KDKFLRINFQ SSRGTVSLGL KRDNLYVVAY
110 120 130 140 150
LAMDNTNVNR AYYFKSEITS AELTALFPEA TTANQKALEY TEDYQSIEKN
160 170 180 190 200
AQITQGDKSR KELGLGIDLL LTFMEAVNKK ARVVKNEARF LLIAIQMTAE
210 220 230 240 250
VARFRYIQNL VTKNFPNKFD SDNKVIQFEV SWRKISTAIY GDAKNGVFNK
260 270 280 290
DYDFGFGKVR QVKDLQMGLL MYLGKPKSSN EANSTAYATT VL
Length:292
Mass (Da):32,811
Last modified:August 1, 1992 - v1
Checksum:iFA143C0E1BE88976
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti72 – 721D → E.

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59255 Genomic DNA. Translation: CAA41948.1.
X69132 Genomic DNA. Translation: CAA48886.1.
X69133 Genomic DNA. Translation: CAA48887.1.
PIRiS16487.
S17933. RLQHG2.
S38527.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59255 Genomic DNA. Translation: CAA41948.1.
X69132 Genomic DNA. Translation: CAA48886.1.
X69133 Genomic DNA. Translation: CAA48887.1.
PIRiS16487.
S17933. RLQHG2.
S38527.

3D structure databases

ProteinModelPortaliP27559.
SMRiP27559. Positions 25-277.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

Allergomei2805. Sap o RIP.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.420.10. 1 hit.
4.10.470.10. 1 hit.
InterProiIPR001574. Ribosome_inactivat_prot.
IPR017988. Ribosome_inactivat_prot_CS.
IPR016138. Ribosome_inactivat_prot_sub1.
IPR016139. Ribosome_inactivat_prot_sub2.
IPR017989. Ribosome_inactivat_prot_subgr.
[Graphical view]
PfamiPF00161. RIP. 1 hit.
[Graphical view]
PRINTSiPR00396. SHIGARICIN.
SUPFAMiSSF56371. SSF56371. 1 hit.
PROSITEiPS00275. SHIGA_RICIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRIP2_SAPOF
AccessioniPrimary (citable) accession number: P27559
Secondary accession number(s): Q41390, Q9SAP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: June 8, 2016
This is version 86 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.