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Protein

CD40 ligand

Gene

Cd40lg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei111 – 1122CleavageBy similarity

GO - Molecular functioni

  1. CD40 receptor binding Source: UniProtKB

GO - Biological processi

  1. B cell differentiation Source: MGI
  2. B cell proliferation Source: UniProtKB
  3. immunoglobulin secretion Source: MGI
  4. inflammatory response Source: UniProtKB
  5. isotype switching Source: UniProtKB
  6. negative regulation of apoptotic process Source: UniProtKB
  7. platelet activation Source: UniProtKB
  8. positive regulation of endothelial cell apoptotic process Source: MGI
  9. positive regulation of interleukin-12 production Source: MGI
  10. regulation of immunoglobulin secretion Source: MGI
  11. signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

ReactomeiREACT_300990. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Names & Taxonomyi

Protein namesi
Recommended name:
CD40 ligand
Short name:
CD40-L
Alternative name(s):
T-cell antigen Gp39
TNF-related activation protein
Short name:
TRAP
Tumor necrosis factor ligand superfamily member 5
CD_antigen: CD154
Cleaved into the following 2 chains:
Gene namesi
Name:Cd40lg
Synonyms:Cd40l, Tnfsf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88337. Cd40lg.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei23 – 4624Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini47 – 260214ExtracellularSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. extracellular space Source: UniProtKB-KW
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 260260CD40 ligand, membrane formPRO_0000034490Add
BLAST
Chaini112 – 260149CD40 ligand, soluble formBy similarityPRO_0000034491Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi177 ↔ 2171 Publication
Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP27548.
PRIDEiP27548.

PTM databases

PhosphoSiteiP27548.

Expressioni

Tissue specificityi

Specifically expressed on activated CD4+ T-lymphocytes.

Gene expression databases

BgeeiP27548.
CleanExiMM_CD40LG.
ExpressionAtlasiP27548. baseline.
GenevestigatoriP27548.

Interactioni

Subunit structurei

Homotrimer.

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CDAmodel-A/B/C115-260[»]
ProteinModelPortaliP27548.
SMRiP27548. Positions 115-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39330.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiP27548.
KOiK03161.
OMAiKIFMYLL.
OrthoDBiEOG7ZPNKR.
PhylomeDBiP27548.
TreeFamiTF332169.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR003263. TNF_ligand_5.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL
60 70 80 90 100
DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL
110 120 130 140 150
NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN
160 170 180 190 200
LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS
210 220 230 240 250
ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV
260
GFSSFGLLKL
Length:260
Mass (Da):29,370
Last modified:August 13, 2001 - v2
Checksum:i7E1AC117473672AD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti198 – 1981S → I in AAC13640 (PubMed:7520529).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.
CCDSiCCDS30152.1.
PIRiS21738.
RefSeqiNP_035746.2. NM_011616.2.
UniGeneiMm.4861.

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.
GeneIDi21947.
KEGGimmu:21947.
UCSCiuc009thb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.
CCDSiCCDS30152.1.
PIRiS21738.
RefSeqiNP_035746.2. NM_011616.2.
UniGeneiMm.4861.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1CDAmodel-A/B/C115-260[»]
ProteinModelPortaliP27548.
SMRiP27548. Positions 115-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466.

PTM databases

PhosphoSiteiP27548.

Proteomic databases

PaxDbiP27548.
PRIDEiP27548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.
GeneIDi21947.
KEGGimmu:21947.
UCSCiuc009thb.1. mouse.

Organism-specific databases

CTDi959.
MGIiMGI:88337. Cd40lg.

Phylogenomic databases

eggNOGiNOG39330.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiP27548.
KOiK03161.
OMAiKIFMYLL.
OrthoDBiEOG7ZPNKR.
PhylomeDBiP27548.
TreeFamiTF332169.

Enzyme and pathway databases

ReactomeiREACT_300990. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

Miscellaneous databases

NextBioi301600.
PROiP27548.
SOURCEiSearch...

Gene expression databases

BgeeiP27548.
CleanExiMM_CD40LG.
ExpressionAtlasiP27548. baseline.
GenevestigatoriP27548.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR003263. TNF_ligand_5.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Spriggs M.K.
    Submitted (MAR-2001) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 198.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 137-260.
    Strain: 129/Sv.
    Tissue: Liver.
  4. Cited for: SIMILARITY TO THE TNF FAMILY.
  5. "A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors."
    Peitsch M.C., Jongeneel C.V.
    Int. Immunol. 5:233-238(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: 3D-STRUCTURE MODELING OF 115-260, DISULFIDE BOND.

Entry informationi

Entry nameiCD40L_MOUSE
AccessioniPrimary (citable) accession number: P27548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 31, 1992
Last sequence update: August 13, 2001
Last modified: March 31, 2015
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.