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P27548

- CD40L_MOUSE

UniProt

P27548 - CD40L_MOUSE

Protein

CD40 ligand

Gene

Cd40lg

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 134 (01 Oct 2014)
      Sequence version 2 (14 Aug 2001)
      Previous versions | rss
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    Functioni

    Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei111 – 1122CleavageBy similarity

    GO - Molecular functioni

    1. CD40 receptor binding Source: UniProtKB

    GO - Biological processi

    1. B cell differentiation Source: MGI
    2. B cell proliferation Source: UniProtKB
    3. immunoglobulin secretion Source: MGI
    4. inflammatory response Source: UniProtKB
    5. isotype switching Source: UniProtKB
    6. negative regulation of apoptotic process Source: UniProtKB
    7. platelet activation Source: UniProtKB
    8. positive regulation of endothelial cell apoptotic process Source: Ensembl
    9. positive regulation of interleukin-12 production Source: Ensembl
    10. regulation of immunoglobulin secretion Source: MGI
    11. signal transduction Source: UniProtKB

    Keywords - Molecular functioni

    Cytokine

    Enzyme and pathway databases

    ReactomeiREACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    CD40 ligand
    Short name:
    CD40-L
    Alternative name(s):
    T-cell antigen Gp39
    TNF-related activation protein
    Short name:
    TRAP
    Tumor necrosis factor ligand superfamily member 5
    CD_antigen: CD154
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Cd40lg
    Synonyms:Cd40l, Tnfsf5
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:88337. Cd40lg.

    Subcellular locationi

    GO - Cellular componenti

    1. external side of plasma membrane Source: MGI
    2. extracellular space Source: UniProtKB-KW
    3. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 260260CD40 ligand, membrane formPRO_0000034490Add
    BLAST
    Chaini112 – 260149CD40 ligand, soluble formBy similarityPRO_0000034491Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi177 ↔ 2171 Publication
    Glycosylationi239 – 2391N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The soluble form derives from the membrane form by proteolytic processing.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiP27548.
    PRIDEiP27548.

    PTM databases

    PhosphoSiteiP27548.

    Expressioni

    Tissue specificityi

    Specifically expressed on activated CD4+ T-lymphocytes.

    Gene expression databases

    ArrayExpressiP27548.
    BgeeiP27548.
    CleanExiMM_CD40LG.
    GenevestigatoriP27548.

    Interactioni

    Subunit structurei

    Homotrimer.

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000033466.

    Structurei

    Secondary structure

    1
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi118 – 1203
    Beta strandi122 – 1276
    Turni130 – 1323
    Turni156 – 1583
    Beta strandi159 – 1613
    Beta strandi166 – 17611
    Beta strandi188 – 1958
    Beta strandi202 – 2098
    Beta strandi220 – 23011
    Beta strandi235 – 2428
    Beta strandi253 – 2608

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1CDAmodel-A/B/C115-260[»]
    ProteinModelPortaliP27548.
    SMRiP27548. Positions 115-260.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2222CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini47 – 260214ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei23 – 4624Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the tumor necrosis factor family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG39330.
    HOGENOMiHOG000111291.
    HOVERGENiHBG079629.
    InParanoidiP27548.
    KOiK03161.
    OMAiFLITQMI.
    OrthoDBiEOG7ZPNKR.
    PhylomeDBiP27548.
    TreeFamiTF332169.

    Family and domain databases

    Gene3Di2.60.120.40. 1 hit.
    InterProiIPR021184. TNF_CS.
    IPR006052. TNF_dom.
    IPR003263. TNF_ligand_5.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view]
    PfamiPF00229. TNF. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016527. TNF_5. 1 hit.
    PRINTSiPR01702. CD40LIGAND.
    SMARTiSM00207. TNF. 1 hit.
    [Graphical view]
    SUPFAMiSSF49842. SSF49842. 1 hit.
    PROSITEiPS00251. TNF_1. 1 hit.
    PS50049. TNF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P27548-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL    50
    DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL 100
    NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN 150
    LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS 200
    ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV 250
    GFSSFGLLKL 260
    Length:260
    Mass (Da):29,370
    Last modified:August 14, 2001 - v2
    Checksum:i7E1AC117473672AD
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti198 – 1981S → I in AAC13640. (PubMed:7520529)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65453 mRNA. Translation: CAA46448.2.
    S71858 Genomic DNA. Translation: AAC13639.1.
    S71861 Genomic DNA. Translation: AAC13640.1.
    CCDSiCCDS30152.1.
    PIRiS21738.
    RefSeqiNP_035746.2. NM_011616.2.
    UniGeneiMm.4861.

    Genome annotation databases

    EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.
    GeneIDi21947.
    KEGGimmu:21947.
    UCSCiuc009thb.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X65453 mRNA. Translation: CAA46448.2 .
    S71858 Genomic DNA. Translation: AAC13639.1 .
    S71861 Genomic DNA. Translation: AAC13640.1 .
    CCDSi CCDS30152.1.
    PIRi S21738.
    RefSeqi NP_035746.2. NM_011616.2.
    UniGenei Mm.4861.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1CDA model - A/B/C 115-260 [» ]
    ProteinModelPortali P27548.
    SMRi P27548. Positions 115-260.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10090.ENSMUSP00000033466.

    PTM databases

    PhosphoSitei P27548.

    Proteomic databases

    PaxDbi P27548.
    PRIDEi P27548.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000033466 ; ENSMUSP00000033466 ; ENSMUSG00000031132 .
    GeneIDi 21947.
    KEGGi mmu:21947.
    UCSCi uc009thb.1. mouse.

    Organism-specific databases

    CTDi 959.
    MGIi MGI:88337. Cd40lg.

    Phylogenomic databases

    eggNOGi NOG39330.
    HOGENOMi HOG000111291.
    HOVERGENi HBG079629.
    InParanoidi P27548.
    KOi K03161.
    OMAi FLITQMI.
    OrthoDBi EOG7ZPNKR.
    PhylomeDBi P27548.
    TreeFami TF332169.

    Enzyme and pathway databases

    Reactomei REACT_202937. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.

    Miscellaneous databases

    NextBioi 301600.
    PROi P27548.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P27548.
    Bgeei P27548.
    CleanExi MM_CD40LG.
    Genevestigatori P27548.

    Family and domain databases

    Gene3Di 2.60.120.40. 1 hit.
    InterProi IPR021184. TNF_CS.
    IPR006052. TNF_dom.
    IPR003263. TNF_ligand_5.
    IPR008983. Tumour_necrosis_fac-like_dom.
    [Graphical view ]
    Pfami PF00229. TNF. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016527. TNF_5. 1 hit.
    PRINTSi PR01702. CD40LIGAND.
    SMARTi SM00207. TNF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49842. SSF49842. 1 hit.
    PROSITEi PS00251. TNF_1. 1 hit.
    PS50049. TNF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Spriggs M.K.
      Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION TO 198.
    3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 137-260.
      Strain: 129/Sv.
      Tissue: Liver.
    4. Cited for: SIMILARITY TO THE TNF FAMILY.
    5. "A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors."
      Peitsch M.C., Jongeneel C.V.
      Int. Immunol. 5:233-238(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: 3D-STRUCTURE MODELING OF 115-260, DISULFIDE BOND.

    Entry informationi

    Entry nameiCD40L_MOUSE
    AccessioniPrimary (citable) accession number: P27548
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 14, 2001
    Last modified: October 1, 2014
    This is version 134 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3