CD40 ligand - Mus musculus (Mouse)

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P27548 (CD40L_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
CD40 ligand
Short name=CD40-L

Alternative name(s):
T-cell antigen Gp39
TNF-related activation protein
Short name=TRAP
Tumor necrosis factor ligand superfamily member 5
CD_antigen=CD154

Gene names

Name:	Cd40lg
Synonyms:	Cd40l, Tnfsf5

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching By similarity.
Subunit structure	Homotrimer.
Subcellular location	Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted.
Tissue specificity	Specifically expressed on activated CD4+ T-lymphocytes.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing By similarity.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal-anchor Transmembrane Transmembrane helix
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell differentiation Inferred from genetic interaction PubMed 14647274. Source: MGI B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB immunoglobulin secretion Inferred from genetic interaction PubMed 15485634. Source: MGI inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB isotype switching Inferred from direct assay PubMed 7529792. Source: UniProtKB negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB platelet activation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of endothelial cell apoptotic process Inferred from electronic annotation. Source: Compara positive regulation of interleukin-12 production Inferred from electronic annotation. Source: Compara regulation of immunoglobulin secretion Inferred from direct assay PubMed 12958312. Source: MGI signal transduction Non-traceable author statement PubMed 7529792. Source: UniProtKB
Cellular_component	external side of plasma membrane Inferred from direct assay PubMed 17082577. Source: MGI extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	CD40 receptor binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

CD40 ligand, membrane form

PRO_0000034490

Chain

112 – 260

149

CD40 ligand, soluble form By similarity

PRO_0000034491

Regions

Topological domain

1 – 22

Cytoplasmic Potential

Transmembrane

23 – 46

Helical; Signal-anchor for type II membrane protein; Potential

Topological domain

47 – 260

214

Extracellular Potential

Sites

Site

111 – 112

Cleavage By similarity

Amino acid modifications

Modified residue

Phosphothreonine Ref.5

Modified residue

Phosphoserine Ref.5

Modified residue

149

Phosphoserine Ref.5

Glycosylation

239

N-linked (GlcNAc...) Potential

Disulfide bond

177 ↔ 217

Ref.6

Experimental info

Sequence conflict

198

S → I in AAC13640. Ref.3

Secondary structure

260

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P27548 [UniParc].

Last modified August 14, 2001. Version 2.
Checksum: 7E1AC117473672AD
Show »

FASTA

260

29,370

        10         20         30         40         50         60 
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL DKVEEEVNLH 

        70         80         90        100        110        120 
EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL NKEEKKENSF EMQRGDEDPQ 

       130        140        150        160        170        180 
IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVMLENGKQL TVKREGLYYV YTQVTFCSNR 

       190        200        210        220        230        240 
EPSSQRPFIV GLWLKPSSGS ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV 

       250        260 
TEASQVIHRV GFSSFGLLKL

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biological characterization of a murine ligand for CD40." Armitage R., Fanslow W., Sato T.A., Clifford K.N., Strockbine L., Macduff B.M., Anderson D.M., Gimpel S.D., Davis-Smith T., Maliszewski C.R., Clark E.A., Smith C.A., Grabstein K.H., Cosman D., Spriggs M.K. Nature 357:80-82(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Spriggs M.K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 198.
[3]	"Structure of the murine CD40 ligand gene." Tsitsikov E.N., Ramesh N., Geha R.S. Mol. Immunol. 31:895-900(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 137-260. Strain: 129/Sv. Tissue: Liver.
[4]	"Emerging cytokine family." Farrah T., Smith C.A. Nature 358:26-26(1992) [PubMed] [Europe PMC] [Abstract] Cited for: SIMILARITY TO THE TNF FAMILY.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-4; SER-6 AND SER-149, MASS SPECTROMETRY. Tissue: Liver.
[6]	"A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors." Peitsch M.C., Jongeneel C.V. Int. Immunol. 5:233-238(1993) [PubMed] [Europe PMC] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 115-260, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.

IPI

IPI00117673.

PIR

S21738.

RefSeq

NP_035746.2. NM_011616.2.

UniGene

Mm.4861.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CDA	model	-	A/B/C	115-260	[»]

ProteinModelPortal

P27548.

SMR

P27548. Positions 115-260.

ModBase

Search...

Protein-protein interaction databases

STRING

10090.ENSMUSP00000033466.

PTM databases

PhosphoSite

P27548.

Proteomic databases

PaxDb

P27548.

PRIDE

P27548.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.

GeneID

21947.

KEGG

mmu:21947.

Organism-specific databases

CTD

959.

MGI

MGI:88337. Cd40lg.

Phylogenomic databases

eggNOG

NOG39330.

HOGENOM

HOG000111291.

HOVERGEN

HBG079629.

InParanoid

P27548.

K03161.

OrthoDB

EOG4G4GRV.

Gene expression databases

ArrayExpress

P27548.

Bgee

P27548.

CleanEx

MM_CD40LG.

Genevestigator

P27548.

GermOnline

ENSMUSG00000031132. Mus musculus.

Family and domain databases

Gene3D

2.60.120.40. 1 hit.

InterPro

IPR006052. TNF.
IPR021184. TNF_CS.
IPR003263. TNF_ligand_5.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]

Pfam

PF00229. TNF. 1 hit.
[Graphical view]

PIRSF

PIRSF016527. TNF_5. 1 hit.

PRINTS

PR01702. CD40LIGAND.

SMART

SM00207. TNF. 1 hit.
[Graphical view]

SUPFAM

SSF49842. TNF_like. 1 hit.

PROSITE

PS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

301600.

SOURCE

Search...

Entry information

Entry name

CD40L_MOUSE

Accession

Primary (citable) accession number: P27548

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 14, 2001
Last modified:	April 3, 2013
This is version 123 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Cleaved into the following 2 chains:

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents