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Protein

CD40 ligand

Gene

Cd40lg

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T-cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF-kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity). Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165).By similarity1 Publication
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Cytokine

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
CD40 ligand
Short name:
CD40-L
Alternative name(s):
T-cell antigen Gp39
TNF-related activation protein
Short name:
TRAP
Tumor necrosis factor ligand superfamily member 5
CD_antigen: CD154
Cleaved into the following 2 chains:
Gene namesi
Name:Cd40lg
Synonyms:Cd40l, Tnfsf5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:88337. Cd40lg.

Subcellular locationi

  • Cell membrane By similarity; Single-pass type II membrane protein By similarity
  • Cell surface By similarity
CD40 ligand, soluble form :
  • Secreted By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 22CytoplasmicSequence analysisAdd BLAST22
Transmembranei23 – 46Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini47 – 260ExtracellularSequence analysisAdd BLAST214

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000344901 – 260CD40 ligand, membrane formAdd BLAST260
ChainiPRO_0000034491112 – 260CD40 ligand, soluble formBy similarityAdd BLAST149

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi177 ↔ 2171 Publication
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

The soluble form derives from the membrane form by proteolytic processing.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei111 – 112CleavageBy similarity2

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP27548.
PRIDEiP27548.

PTM databases

iPTMnetiP27548.
PhosphoSitePlusiP27548.

Expressioni

Tissue specificityi

Specifically expressed on activated CD4+ T-lymphocytes.

Gene expression databases

BgeeiENSMUSG00000031132.
CleanExiMM_CD40LG.
ExpressionAtlasiP27548. baseline and differential.
GenevisibleiP27548. MM.

Interactioni

Subunit structurei

Homotrimer (PubMed:8095800). Interacts with CD28 (By similarity).1 PublicationBy similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDAmodel-A/B/C115-260[»]
ProteinModelPortaliP27548.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the tumor necrosis factor family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IVYF. Eukaryota.
ENOG4111TET. LUCA.
GeneTreeiENSGT00510000048489.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiP27548.
KOiK03161.
OMAiSMKIFMY.
OrthoDBiEOG091G0I9G.
PhylomeDBiP27548.
TreeFamiTF332169.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27548-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL
60 70 80 90 100
DKVEEEVNLH EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL
110 120 130 140 150
NKEEKKENSF EMQRGDEDPQ IAAHVVSEAN SNAASVLQWA KKGYYTMKSN
160 170 180 190 200
LVMLENGKQL TVKREGLYYV YTQVTFCSNR EPSSQRPFIV GLWLKPSSGS
210 220 230 240 250
ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV TEASQVIHRV
260
GFSSFGLLKL
Length:260
Mass (Da):29,370
Last modified:August 14, 2001 - v2
Checksum:i7E1AC117473672AD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198S → I in AAC13640 (PubMed:7520529).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.
CCDSiCCDS30152.1.
PIRiS21738.
RefSeqiNP_035746.2. NM_011616.2.
UniGeneiMm.4861.

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.
GeneIDi21947.
KEGGimmu:21947.
UCSCiuc009thb.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.
CCDSiCCDS30152.1.
PIRiS21738.
RefSeqiNP_035746.2. NM_011616.2.
UniGeneiMm.4861.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1CDAmodel-A/B/C115-260[»]
ProteinModelPortaliP27548.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033466.

PTM databases

iPTMnetiP27548.
PhosphoSitePlusiP27548.

Proteomic databases

PaxDbiP27548.
PRIDEiP27548.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033466; ENSMUSP00000033466; ENSMUSG00000031132.
GeneIDi21947.
KEGGimmu:21947.
UCSCiuc009thb.1. mouse.

Organism-specific databases

CTDi959.
MGIiMGI:88337. Cd40lg.

Phylogenomic databases

eggNOGiENOG410IVYF. Eukaryota.
ENOG4111TET. LUCA.
GeneTreeiENSGT00510000048489.
HOGENOMiHOG000111291.
HOVERGENiHBG079629.
InParanoidiP27548.
KOiK03161.
OMAiSMKIFMY.
OrthoDBiEOG091G0I9G.
PhylomeDBiP27548.
TreeFamiTF332169.

Enzyme and pathway databases

ReactomeiR-MMU-198933. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
R-MMU-5668541. TNFR2 non-canonical NF-kB pathway.
R-MMU-5676594. TNF receptor superfamily (TNFSF) members mediating non-canonical NF-kB pathway.

Miscellaneous databases

PROiP27548.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031132.
CleanExiMM_CD40LG.
ExpressionAtlasiP27548. baseline and differential.
GenevisibleiP27548. MM.

Family and domain databases

Gene3Di2.60.120.40. 1 hit.
InterProiIPR003263. CD40L.
IPR021184. TNF_CS.
IPR006052. TNF_dom.
IPR008983. Tumour_necrosis_fac-like_dom.
[Graphical view]
PfamiPF00229. TNF. 1 hit.
[Graphical view]
PIRSFiPIRSF016527. TNF_5. 1 hit.
PRINTSiPR01702. CD40LIGAND.
SMARTiSM00207. TNF. 1 hit.
[Graphical view]
SUPFAMiSSF49842. SSF49842. 1 hit.
PROSITEiPS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD40L_MOUSE
AccessioniPrimary (citable) accession number: P27548
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 14, 2001
Last modified: November 2, 2016
This is version 153 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.