Reviewed, UniProtKB/Swiss-Prot P27548 (CD40L_MOUSE)

Names and origin

Protein names

Recommended name:
    CD40 ligand
      Short name=CD40-L
Alternative name(s):
    Tumor necrosis factor ligand superfamily member 5
    TNF-related activation protein
      Short name=TRAP
    T-cell antigen Gp39
    CD_antigen=CD154
Cleaved into the following 2 chains:
    1- Recommended name:
            CD40 ligand, membrane form
    2- Recommended name:
            CD40 ligand, soluble form

Gene names

Name:	Cd40lg
Synonyms:	Cd40l, Tnfsf5

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Cytokine that binds to TNFRSF5. Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching By similarity.
Subunit structure	Homotrimer.
Subcellular location	Cell membrane; Single-pass type II membrane protein. CD40 ligand, soluble form: Secreted.
Tissue specificity	Specifically expressed on activated CD4+ T-lymphocytes.
Post-translational modification	The soluble form derives from the membrane form by proteolytic processing By similarity.
Sequence similarities	Belongs to the tumor necrosis factor family.

Ontologies

Keywords
Cellular component	Cell membrane Membrane Secreted
Domain	Signal-anchor Transmembrane
Molecular function	Cytokine
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure
Gene Ontology (GO)
Biological process	B cell differentiation Inferred from genetic interaction. Source: MGI B cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB anti-apoptosis Inferred from sequence or structural similarity. Source: UniProtKB immunoglobulin secretion Inferred from genetic interaction. Source: MGI inflammatory response Inferred from sequence or structural similarity. Source: UniProtKB isotype switching Inferred from direct assay. Source: UniProtKB platelet activation Inferred from sequence or structural similarity. Source: UniProtKB regulation of immunoglobulin secretion Inferred from direct assay. Source: MGI signal transduction Non-traceable author statement. Source: UniProtKB
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	CD40 receptor binding Inferred from sequence or structural similarity. Source: UniProtKB cytokine activity Inferred from electronic annotation. Source: UniProtKB-KW tumor necrosis factor receptor binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 260

260

CD40 ligand, membrane form

PRO_0000034490

Chain

112 – 260

149

CD40 ligand, soluble form By similarity

PRO_0000034491

Regions

Topological domain

1 – 22

22

Cytoplasmic Potential

Transmembrane

23 – 46

24

Signal-anchor for type II membrane protein Potential

Topological domain

47 – 260

214

Extracellular Potential

Sites

Site

111 – 112

2

Cleavage By similarity

Amino acid modifications

Modified residue

4

1

Phosphothreonine Ref.5

Modified residue

6

1

Phosphoserine Ref.5

Modified residue

149

1

Phosphoserine Ref.5

Glycosylation

239

1

N-linked (GlcNAc...) Potential

Disulfide bond

177 ↔ 217

Ref.6

Experimental info

Sequence conflict

198

1

S → I in AAC13640. Ref.3

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

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.

.

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.

.

.

.

.

260

Helix Strand Turn

Details...

Beta strand

118 – 120

3

Beta strand

122 – 127

6

Turn

130 – 132

3

Turn

156 – 158

3

Beta strand

159 – 161

3

Beta strand

166 – 176

11

Beta strand

188 – 195

8

Beta strand

202 – 209

8

Beta strand

220 – 230

11

Beta strand

235 – 242

8

Beta strand

253 – 260

8

Sequences

	Sequence		Length	Mass (Da)
	P27548-1 [UniParc]. Last modified August 14, 2001. Version 2. Checksum: 7E1AC117473672AD	FASTA	260	29,370
	10 20 30 40 50 60 MIETYSQPSP RSVATGLPAS MKIFMYLLTV FLITQMIGSV LFAVYLHRRL DKVEEEVNLH 70 80 90 100 110 120 EDFVFIKKLK RCNKGEGSLS LLNCEEMRRQ FEDLVKDITL NKEEKKENSF EMQRGDEDPQ 130 140 150 160 170 180 IAAHVVSEAN SNAASVLQWA KKGYYTMKSN LVMLENGKQL TVKREGLYYV YTQVTFCSNR 190 200 210 220 230 240 EPSSQRPFIV GLWLKPSSGS ERILLKAANT HSSSQLCEQQ SVHLGGVFEL QAGASVFVNV 250 260 TEASQVIHRV GFSSFGLLKL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biological characterization of a murine ligand for CD40." Armitage R., Fanslow W., Sato T.A., Clifford K.N., Strockbine L., Macduff B.M., Anderson D.M., Gimpel S.D., Davis-Smith T., Maliszewski C.R., Clark E.A., Smith C.A., Grabstein K.H., Cosman D., Spriggs M.K. Nature 357:80-82(1992) [PubMed: 1374165] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Spriggs M.K. Submitted (APR-2001) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO 198.
[3]	"Structure of the murine CD40 ligand gene." Tsitsikov E.N., Ramesh N., Geha R.S. Mol. Immunol. 31:895-900(1994) [PubMed: 7520529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 137-260. Strain: 129/Sv. Tissue: Liver.
[4]	"Emerging cytokine family." Farrah T., Smith C.A. Nature 358:26-26(1992) [PubMed: 1377364] [Abstract] Cited for: SIMILARITY TO THE TNF FAMILY.
[5]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-4; SER-6 AND SER-149, MASS SPECTROMETRY. Tissue: Liver.
[6]	"A 3-D model for the CD40 ligand predicts that it is a compact trimer similar to the tumor necrosis factors." Peitsch M.C., Jongeneel C.V. Int. Immunol. 5:233-238(1993) [PubMed: 8095800] [Abstract] Cited for: 3D-STRUCTURE MODELING OF 115-260, DISULFIDE BOND.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X65453 mRNA. Translation: CAA46448.2.
S71858 Genomic DNA. Translation: AAC13639.1.
S71861 Genomic DNA. Translation: AAC13640.1.

IPI

IPI00117673.

PIR

S21738.

RefSeq

NP_035746.2.

UniGene

Mm.4861

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1CDA	model	-	A/B/C	115-260	[»]

SMR

P27548. Positions 115-260.

ModBase

Search...

PTM databases

PhosphoSite

P27548.

Proteomic databases

PRIDE

P27548.

Genome annotation databases

Ensembl

ENSMUSG00000031132. Mus musculus. [Contig view]

GeneID

21947.

KEGG

mmu:21947.

Organism-specific databases

MGI

MGI:88337. Cd40lg.

Phylogenomic databases

HOGENOM

P27548.

HOVERGEN

P27548.

OMA

P27548. KIFMYLL.

Gene expression databases

ArrayExpress

P27548.

Bgee

P27548.

CleanEx

MM_CD40LG.

GermOnline

ENSMUSG00000031132. Mus musculus.

Family and domain databases

InterPro

IPR003263. TNF_5.
IPR006052. TNF_family.
IPR008983. Tumour_necrosis_fac-like.
[Graphical view]

Gene3D

G3DSA:2.60.120.40. Tumour_necrosis_fac-like. 1 hit.

Pfam

PF00229. TNF. 1 hit.
[Graphical view]

PIRSF

PIRSF016527. TNF_5. 1 hit.

PRINTS

PR01702. CD40LIGAND.

ProDom

PD002012. TNF_subf. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00207. TNF. 1 hit.
[Graphical view]

PROSITE

PS00251. TNF_1. 1 hit.
PS50049. TNF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

301600.

SOURCE

Search...

Entry information

Entry name

CD40L_MOUSE

Accession

Primary (citable) accession number: P27548

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 14, 2001
Last modified:	June 16, 2009
This is version 93 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)