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Protein

Microtubule-associated protein 4

Gene

Map4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-neuronal microtubule-associated protein. Promotes microtubule assembly.

GO - Molecular functioni

  • microtubule binding Source: MGI
  • poly(A) RNA binding Source: MGI

GO - Biological processi

  • cell division Source: MGI
  • establishment of spindle orientation Source: MGI
  • microtubule sliding Source: MGI
  • mitotic spindle organization Source: MGI
  • negative regulation of non-motile cilium assembly Source: MGI
  • neuron projection development Source: GO_Central
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Microtubule-associated protein 4
Short name:
MAP-4
Gene namesi
Name:Map4
Synonyms:Mtap4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:97178. Map4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000727522 – 1125Microtubule-associated protein 4Add BLAST1124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei5PhosphoserineBy similarity1
Modified residuei60PhosphoserineBy similarity1
Modified residuei99PhosphoserineBy similarity1
Modified residuei254PhosphoserineCombined sources1
Modified residuei260PhosphothreonineCombined sources1
Modified residuei277PhosphothreonineBy similarity1
Modified residuei349PhosphothreonineBy similarity1
Modified residuei381PhosphoserineCombined sources1
Modified residuei410PhosphothreonineBy similarity1
Modified residuei447PhosphothreonineCombined sources1
Modified residuei475PhosphoserineCombined sources1
Modified residuei494PhosphothreonineBy similarity1
Modified residuei503PhosphothreonineCombined sources1
Modified residuei506PhosphoserineCombined sources1
Modified residuei511PhosphothreonineCombined sources1
Modified residuei512PhosphoserineCombined sources1
Modified residuei517PhosphoserineCombined sources1
Modified residuei519PhosphoserineBy similarity1
Modified residuei598PhosphoserineCombined sources1
Modified residuei617PhosphoserineCombined sources1
Modified residuei658PhosphothreonineCombined sources1
Modified residuei667PhosphoserineCombined sources1
Modified residuei684PhosphoserineBy similarity1
Modified residuei694PhosphoserineBy similarity1
Modified residuei760PhosphoserineCombined sources1
Modified residuei798PhosphoserineBy similarity1
Modified residuei826PhosphoserineBy similarity1
Modified residuei901PhosphoserineCombined sources1
Modified residuei914PhosphoserineCombined sources1
Modified residuei915PhosphothreonineBy similarity1
Modified residuei973PhosphoserineBy similarity1
Modified residuei1046PhosphoserineCombined sources1
Modified residuei1118PhosphoserineBy similarity1
Modified residuei1124PhosphoserineBy similarity1
Isoform 4 (identifier: P27546-4)
Modified residuei333PhosphoserineBy similarityCurated1
Modified residuei334Phosphoserine By similarityBy similarityCurated1

Post-translational modificationi

Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Phosphorylation on Ser-760 negatively regulates MAP4 activity to promote microtubule assembly. Isoform 4 is phosphorylated on Ser-333 and Ser-334 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP27546.
PaxDbiP27546.
PeptideAtlasiP27546.
PRIDEiP27546.

PTM databases

iPTMnetiP27546.
PhosphoSitePlusiP27546.
SwissPalmiP27546.

Expressioni

Tissue specificityi

Testis, striated and cardiac muscle.

Gene expression databases

BgeeiENSMUSG00000032479.
CleanExiMM_MTAP4.
ExpressionAtlasiP27546. baseline and differential.
GenevisibleiP27546. MM.

Interactioni

Subunit structurei

Interacts with SEPT2; this interaction impedes tubulin-binding. Interacts with TRAF3IP1 (PubMed:26487268).By similarity1 Publication

GO - Molecular functioni

  • microtubule binding Source: MGI

Protein-protein interaction databases

BioGridi201586. 6 interactors.
IntActiP27546. 9 interactors.
MINTiMINT-1861472.
STRINGi10090.ENSMUSP00000035055.

Structurei

3D structure databases

ProteinModelPortaliP27546.
SMRiP27546.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati896 – 926Tau/MAP 1Add BLAST31
Repeati965 – 995Tau/MAP 2Add BLAST31
Repeati996 – 1026Tau/MAP 3Add BLAST31
Repeati1027 – 1058Tau/MAP 4Add BLAST32

Sequence similaritiesi

Contains 4 Tau/MAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG410IG6M. Eukaryota.
ENOG4111J07. LUCA.
GeneTreeiENSGT00530000063491.
HOVERGENiHBG006323.
InParanoidiP27546.
KOiK10431.
PhylomeDBiP27546.

Family and domain databases

InterProiIPR027324. MAP2/MAP4/Tau.
IPR027323. MAP4.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERiPTHR11501. PTHR11501. 2 hits.
PTHR11501:SF16. PTHR11501:SF16. 2 hits.
PfamiPF00418. Tubulin-binding. 4 hits.
[Graphical view]
PROSITEiPS00229. TAU_MAP_1. 4 hits.
PS51491. TAU_MAP_2. 4 hits.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P27546-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADLSLVDAL TEPPPEIEGE IKRDFMAALE AEPYDDIVGE TVEKTEFIPL
60 70 80 90 100
LDGDEKTGNS ESKKKPCLDT SQVEGIPSSK PTLLANGDHG MEGNNTAGSP
110 120 130 140 150
TDFLEERVDY PDYQSSQNWP EDASFCFQPQ QVLDTDQAEP FNEHRDDGLA
160 170 180 190 200
DLLFVSSGPT NASAFTERDN PSEDSYGMLP CDSFASTAVV SQEWSVGAPN
210 220 230 240 250
SPCSESCVSP EVTIETLQPA TELSKAAEVE SVKEQLPAKA LETMAEQTTD
260 270 280 290 300
VVHSPSTDTT PGPDTEAALA KDIEEITKPD VILANVTQPS TESDMFLAQD
310 320 330 340 350
MELLTGTEAA HANNIILPTE PDESSTKDVA PPMEEEIVPG NDTTSPKETE
360 370 380 390 400
TTLPIKMDLA PPEDVLLTKE TELAPAKGMV SLSEIEEALA KNDESSAEIP
410 420 430 440 450
VAQETVVSET EVVLATEVVL PSDPITTLTK DVTLPLEAER PLVTDMTPSL
460 470 480 490 500
ETEMTLGKET APPTETNLGM AKDMSPLPES EVTLGKDVVI LPETKVAEFN
510 520 530 540 550
NVTPLSEEEV TSVKDMSPSA ETEAPLAKNA DLHSGTELIV DNSMAPASDL
560 570 580 590 600
ALPLETKVAT VPIKDKGTVQ TEEKPREDSQ LASMQHKGQS TVPPCTASPE
610 620 630 640 650
PVKAAEQMST LPIDAPSPLE NLEQKETPGS QPSEPCSGVS RQEEAKAAVG
660 670 680 690 700
VTGNDITTPP NKEPPPSPEK KAKPLATTQP AKTSTSKAKT QPTSLPKQPA
710 720 730 740 750
PTTSGGLNKK PMSLASGSVP AAPHKRPAAA TATARPSTLP ARDVKPKPIT
760 770 780 790 800
EAKVAEKRTS PSKPSSAPAL KPGPKTTPTV SKATSPSTLV STGPSSRSPA
810 820 830 840 850
TTLPKRPTSI KTEGKPADVK RMTAKSASAD LSRSKTTSAS SVKRNTTPTG
860 870 880 890 900
AAPPAGMTST RVKPMSAPSR SSGALSVDKK PTSTKPSSSA PRVSRLATTV
910 920 930 940 950
SAPDLKSVRS KVGSTENIKH QPGGGRAKVE KKTEAATTAG KPEPNAVTKA
960 970 980 990 1000
AGSIASAQKP PAGKVQIVSK KVSYSHIQSK CGSKDNIKHV PGGGNVQIQN
1010 1020 1030 1040 1050
KKVDISKVSS KCGSKANIKH KPGGGDVKIE SQKLNFKEKA QAKVGSLDNV
1060 1070 1080 1090 1100
GHLPAGGAVK TEGGGSEALP CPGPPAGEEP VIPEAAPDAG APTSASGLSG
1110 1120
HTTLSGGGDQ REPQTLDSQI QETSI
Length:1,125
Mass (Da):117,429
Last modified:June 12, 2007 - v3
Checksum:iE948B7F1F5B903E9
GO
Isoform 2 (identifier: P27546-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1124-1125: SI → N

Show »
Length:1,124
Mass (Da):117,343
Checksum:i2947F1F5B903E9EC
GO
Isoform 3 (identifier: P27546-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     927-964: Missing.
     1124-1125: SI → N

Show »
Length:1,086
Mass (Da):113,656
Checksum:i476A250416BBACFD
GO
Isoform 4 (identifier: P27546-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MADLSLVDALTEPPPEIEGEIKRDFM → MSLPEKQPAALT
     30-259: EAEPYDDIVG...DVVHSPSTDT → AAEDEQLSKG...YSVTQSPQAA
     263-419: PDTEAALAKD...TEVVLATEVV → KEKNGLVSSC...KYPELPAREE
     424-531: PITTLTKDVT...TEAPLAKNAD → LLPPTSSPMP...KKELGIAGLY
     535-625: GTELIVDNSM...PSPLENLEQK → KLEQIPEGSH...TQSIPSLLLE
     629-637: GSQPSEPCS → RD
     927-964: Missing.
     965-995: Missing.
     1124-1125: SI → N

Show »
Length:902
Mass (Da):94,517
Checksum:i031215FB486E6845
GO

Sequence cautioni

The sequence AAH42645 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE27434 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti225K → E in AAH55364 (PubMed:15489334).Curated1
Sequence conflicti252V → A in AAH55364 (PubMed:15489334).Curated1
Sequence conflicti394 – 395ES → VR in AAA16372 (PubMed:1718985).Curated2
Sequence conflicti416T → I in AAH55332 (PubMed:15489334).Curated1
Sequence conflicti955A → V in AAH55364 (PubMed:15489334).Curated1
Sequence conflicti982G → V in AAA16372 (PubMed:1718985).Curated1
Sequence conflicti993G → C in AAA16372 (PubMed:1718985).Curated1
Sequence conflicti1053L → F in AAA16372 (PubMed:1718985).Curated1
Sequence conflicti1060K → KV in AAH44654 (PubMed:15489334).Curated1
Sequence conflicti1060K → KV in AAH55364 (PubMed:15489334).Curated1
Sequence conflicti1089A → R in AAA16372 (PubMed:1718985).Curated1
Isoform 4 (identifier: P27546-4)
Sequence conflicti435K → E in BAE23650 (PubMed:16141072).By similarityCurated1
Sequence conflicti435K → E in BAE22377 (PubMed:16141072).By similarityCurated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0260891 – 26MADLS…KRDFM → MSLPEKQPAALT in isoform 4. 2 PublicationsAdd BLAST26
Alternative sequenceiVSP_02609030 – 259EAEPY…PSTDT → AAEDEQLSKGNPPECGMDSR KEIGQDGFEWQRTEGKLNEI GLNVSMDGQLKDRLVKNSSF LEQNKLGFFEGKLDKELSIE KPNKAYQETSGHLESGYVIS GTCQPSEGNLVHQKAAEFHP GLTEGKDKAATVQGKVAGKS GLEIKSQPDLNFPGAADTLT QHGEEQETSAWNANFYSVTQ SPQAA in isoform 4. 2 PublicationsAdd BLAST230
Alternative sequenceiVSP_026091263 – 419PDTEA…ATEVV → KEKNGLVSSCSVTGVMSDNS GQLNNKSPLLVAITHPDPTS EHLPTTSPPITMVEFTQENL NAGQDKELEKLRSSEEGPML DQVPQQKKAIRRALSECYHL SVPPAVNLVDKYPELPAREE in isoform 4. 2 PublicationsAdd BLAST157
Alternative sequenceiVSP_026092424 – 531PITTL…AKNAD → LLPPTSSPMPSPMPRKLGVP AMRRSMTVAEDQSASCRLSA GELASLSASQVPTALTFEEP VAKEREEQIHFSNDSNSSGK KELGIAGLY in isoform 4. 2 PublicationsAdd BLAST108
Alternative sequenceiVSP_026093535 – 625GTELI…NLEQK → KLEQIPEGSHKGKGQKNTGE TRVDSCPFICLGGEKQLMAL AGKKEIEVTATQSIPSLLLE in isoform 4. 2 PublicationsAdd BLAST91
Alternative sequenceiVSP_026094629 – 637GSQPSEPCS → RD in isoform 4. 2 Publications9
Alternative sequenceiVSP_026095927 – 964Missing in isoform 3 and isoform 4. 2 PublicationsAdd BLAST38
Alternative sequenceiVSP_026096965 – 995Missing in isoform 4. 2 PublicationsAdd BLAST31
Alternative sequenceiVSP_0260971124 – 1125SI → N in isoform 2, isoform 3 and isoform 4. 2 Publications2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72414 mRNA. Translation: AAA16372.1.
AK134996 mRNA. Translation: BAE22377.1.
AK138416 mRNA. Translation: BAE23650.1.
AK146790 mRNA. Translation: BAE27434.1. Different initiation.
BC042645 mRNA. Translation: AAH42645.1. Different initiation.
BC044654 mRNA. Translation: AAH44654.1.
BC050893 mRNA. Translation: AAH50893.1.
BC055332 mRNA. Translation: AAH55332.1.
BC055364 mRNA. Translation: AAH55364.1.
CCDSiCCDS57704.1. [P27546-1]
CCDS81083.1. [P27546-4]
PIRiB41206.
RefSeqiNP_001192259.1. NM_001205330.1. [P27546-1]
NP_001192261.1. NM_001205332.1. [P27546-3]
NP_001298092.1. NM_001311163.1.
NP_001298093.1. NM_001311164.1.
NP_032659.2. NM_008633.4.
XP_017168653.1. XM_017313164.1. [P27546-2]
UniGeneiMm.217318.
Mm.443428.
Mm.484451.

Genome annotation databases

EnsembliENSMUST00000035055; ENSMUSP00000035055; ENSMUSG00000032479. [P27546-1]
ENSMUST00000165876; ENSMUSP00000132662; ENSMUSG00000032479. [P27546-2]
GeneIDi17758.
KEGGimmu:17758.
UCSCiuc009rsz.3. mouse. [P27546-2]
uc009rta.3. mouse. [P27546-1]
uc009rtb.3. mouse. [P27546-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M72414 mRNA. Translation: AAA16372.1.
AK134996 mRNA. Translation: BAE22377.1.
AK138416 mRNA. Translation: BAE23650.1.
AK146790 mRNA. Translation: BAE27434.1. Different initiation.
BC042645 mRNA. Translation: AAH42645.1. Different initiation.
BC044654 mRNA. Translation: AAH44654.1.
BC050893 mRNA. Translation: AAH50893.1.
BC055332 mRNA. Translation: AAH55332.1.
BC055364 mRNA. Translation: AAH55364.1.
CCDSiCCDS57704.1. [P27546-1]
CCDS81083.1. [P27546-4]
PIRiB41206.
RefSeqiNP_001192259.1. NM_001205330.1. [P27546-1]
NP_001192261.1. NM_001205332.1. [P27546-3]
NP_001298092.1. NM_001311163.1.
NP_001298093.1. NM_001311164.1.
NP_032659.2. NM_008633.4.
XP_017168653.1. XM_017313164.1. [P27546-2]
UniGeneiMm.217318.
Mm.443428.
Mm.484451.

3D structure databases

ProteinModelPortaliP27546.
SMRiP27546.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201586. 6 interactors.
IntActiP27546. 9 interactors.
MINTiMINT-1861472.
STRINGi10090.ENSMUSP00000035055.

PTM databases

iPTMnetiP27546.
PhosphoSitePlusiP27546.
SwissPalmiP27546.

Proteomic databases

MaxQBiP27546.
PaxDbiP27546.
PeptideAtlasiP27546.
PRIDEiP27546.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035055; ENSMUSP00000035055; ENSMUSG00000032479. [P27546-1]
ENSMUST00000165876; ENSMUSP00000132662; ENSMUSG00000032479. [P27546-2]
GeneIDi17758.
KEGGimmu:17758.
UCSCiuc009rsz.3. mouse. [P27546-2]
uc009rta.3. mouse. [P27546-1]
uc009rtb.3. mouse. [P27546-3]

Organism-specific databases

CTDi4134.
MGIiMGI:97178. Map4.

Phylogenomic databases

eggNOGiENOG410IG6M. Eukaryota.
ENOG4111J07. LUCA.
GeneTreeiENSGT00530000063491.
HOVERGENiHBG006323.
InParanoidiP27546.
KOiK10431.
PhylomeDBiP27546.

Miscellaneous databases

PROiP27546.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032479.
CleanExiMM_MTAP4.
ExpressionAtlasiP27546. baseline and differential.
GenevisibleiP27546. MM.

Family and domain databases

InterProiIPR027324. MAP2/MAP4/Tau.
IPR027323. MAP4.
IPR001084. MAP_tubulin-bd_rpt.
[Graphical view]
PANTHERiPTHR11501. PTHR11501. 2 hits.
PTHR11501:SF16. PTHR11501:SF16. 2 hits.
PfamiPF00418. Tubulin-binding. 4 hits.
[Graphical view]
PROSITEiPS00229. TAU_MAP_1. 4 hits.
PS51491. TAU_MAP_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMAP4_MOUSE
AccessioniPrimary (citable) accession number: P27546
Secondary accession number(s): Q05BJ2
, Q3UIS2, Q3UUH5, Q3UY36, Q7TPC6, Q7TPD4, Q80YQ5, Q8CFP5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: June 12, 2007
Last modified: November 30, 2016
This is version 140 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.