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P27544

- CERS1_HUMAN

UniProt

P27544 - CERS1_HUMAN

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Protein

Ceramide synthase 1

Gene

CERS1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing mainly one fatty acid donor (N-linked stearoyl- (C18) ceramide) in a fumonisin B1-independent manner By similarity.By similarity

GO - Molecular functioni

  1. sphingosine N-acyltransferase activity Source: HGNC

GO - Biological processi

  1. cellular response to dithiothreitol Source: UniProt
  2. cellular response to drug Source: UniProt
  3. cellular response to mycotoxin Source: UniProt
  4. cellular response to UV-A Source: UniProt
  5. ceramide biosynthetic process Source: UniProt
  6. negative regulation of telomerase activity Source: UniProt
  7. small molecule metabolic process Source: Reactome
  8. sphingolipid biosynthetic process Source: UniProt
  9. sphingolipid metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER66-34367.
ReactomeiREACT_115810. Sphingolipid de novo biosynthesis.

Names & Taxonomyi

Protein namesi
Recommended name:
Ceramide synthase 1
Short name:
CerS1
Alternative name(s):
LAG1 longevity assurance homolog 1
Longevity assurance gene 1 protein homolog 1
Protein UOG-1
Gene namesi
Name:CERS1
Synonyms:LAG1, LASS1, UOG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:14253. CERS1.

Subcellular locationi

Isoform 1 : Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity. Golgi apparatus membrane By similarity; Multi-pass membrane protein By similarity
Note: Isoform 1 may recycle from the Golgi to the endoplasmic reticulum.

GO - Cellular componenti

  1. endoplasmic reticulum Source: UniProt
  2. endoplasmic reticulum membrane Source: Reactome
  3. Golgi apparatus Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. intracellular membrane-bounded organelle Source: BHF-UCL
  6. membrane Source: UniProt
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA30299.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 350349Ceramide synthase 1PRO_0000185507Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine1 Publication

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP27544.
PaxDbiP27544.
PRIDEiP27544.

PTM databases

PhosphoSiteiP27544.

Expressioni

Gene expression databases

BgeeiP27544.
CleanExiHS_LASS1.
ExpressionAtlasiP27544. baseline and differential.
GenevestigatoriP27544.

Organism-specific databases

HPAiHPA045724.

Interactioni

Protein-protein interaction databases

BioGridi115941. 2 interactions.
IntActiP27544. 2 interactions.
MINTiMINT-8247548.
STRINGi9606.ENSP00000402697.

Structurei

3D structure databases

ProteinModelPortaliP27544.
ModBaseiSearch...
MobiDBiSearch...

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei53 – 7321HelicalSequence AnalysisAdd
BLAST
Transmembranei103 – 12321HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16821HelicalSequence AnalysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence AnalysisAdd
BLAST
Transmembranei287 – 30721HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini97 – 311215TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG306914.
GeneTreeiENSGT00550000074401.
HOVERGENiHBG052309.
InParanoidiP27544.
KOiK04710.
OMAiKVLTGQM.
OrthoDBiEOG7ZD1VB.
PhylomeDBiP27544.
TreeFamiTF314319.

Family and domain databases

InterProiIPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view]
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
PIRSFiPIRSF005225. LAG1_LAC1. 1 hit.
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P27544-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAAGPAAGP TGPEPMPSYA QLVQRGWGSA LAAARGCTDC GWGLARRGLA
60 70 80 90 100
EHAHLAPPEL LLLALGALGW TALRSAATAR LFRPLAKRCC LQPRDAAKMP
110 120 130 140 150
ESAWKFLFYL GSWSYSAYLL FGTDYPFFHD PPSVFYDWTP GMAVPRDIAA
160 170 180 190 200
AYLLQGSFYG HSIYATLYMD TWRKDSVVML LHHVVTLILI VSSYAFRYHN
210 220 230 240 250
VGILVLFLHD ISDVQLEFTK LNIYFKSRGG SYHRLHALAA DLGCLSFGFS
260 270 280 290 300
WFWFRLYWFP LKVLYATSHC SLRTVPDIPF YFFFNALLLL LTLMNLYWFL
310 320 330 340 350
YIVAFAAKVL TGQVHELKDL REYDTAEAQS LKPSKAEKPL RNGLVKDKRF
Length:350
Mass (Da):39,536
Last modified:August 1, 1992 - v1
Checksum:iF102C12C47DB4162
GO
Isoform 2 (identifier: P27544-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     338-350: Missing.

Note: No experimental confirmation available.

Show »
Length:337
Mass (Da):37,983
Checksum:i563C0D539D8E1E5C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti111 – 1111G → C in AAH22450. (PubMed:15489334)Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei338 – 35013Missing in isoform 2. 1 PublicationVSP_003049Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62302 mRNA. Translation: AAA58500.1.
AF105009
, AF105005, AF105006, AF105007, AF105008 Genomic DNA. Translation: AAD16892.1.
AC005197 Genomic DNA. Translation: AAC24611.1.
AC003972 Genomic DNA. No translation available.
BC022450 mRNA. Translation: AAH22450.1.
CCDSiCCDS46021.1. [P27544-2]
PIRiD39364.
RefSeqiNP_067090.1. NM_021267.4. [P27544-1]
NP_937850.1. NM_198207.2. [P27544-2]
UniGeneiHs.412355.

Genome annotation databases

EnsembliENST00000429504; ENSP00000389044; ENSG00000223802. [P27544-2]
GeneIDi10715.
KEGGihsa:10715.
UCSCiuc002nki.1. human. [P27544-1]

Polymorphism databases

DMDMi137046.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M62302 mRNA. Translation: AAA58500.1 .
AF105009
, AF105005 , AF105006 , AF105007 , AF105008 Genomic DNA. Translation: AAD16892.1 .
AC005197 Genomic DNA. Translation: AAC24611.1 .
AC003972 Genomic DNA. No translation available.
BC022450 mRNA. Translation: AAH22450.1 .
CCDSi CCDS46021.1. [P27544-2 ]
PIRi D39364.
RefSeqi NP_067090.1. NM_021267.4. [P27544-1 ]
NP_937850.1. NM_198207.2. [P27544-2 ]
UniGenei Hs.412355.

3D structure databases

ProteinModelPortali P27544.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115941. 2 interactions.
IntActi P27544. 2 interactions.
MINTi MINT-8247548.
STRINGi 9606.ENSP00000402697.

PTM databases

PhosphoSitei P27544.

Polymorphism databases

DMDMi 137046.

Proteomic databases

MaxQBi P27544.
PaxDbi P27544.
PRIDEi P27544.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000429504 ; ENSP00000389044 ; ENSG00000223802 . [P27544-2 ]
GeneIDi 10715.
KEGGi hsa:10715.
UCSCi uc002nki.1. human. [P27544-1 ]

Organism-specific databases

CTDi 10715.
GeneCardsi GC19M018982.
HGNCi HGNC:14253. CERS1.
HPAi HPA045724.
MIMi 606919. gene.
neXtProti NX_P27544.
PharmGKBi PA30299.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG306914.
GeneTreei ENSGT00550000074401.
HOVERGENi HBG052309.
InParanoidi P27544.
KOi K04710.
OMAi KVLTGQM.
OrthoDBi EOG7ZD1VB.
PhylomeDBi P27544.
TreeFami TF314319.

Enzyme and pathway databases

BioCyci MetaCyc:MONOMER66-34367.
Reactomei REACT_115810. Sphingolipid de novo biosynthesis.

Miscellaneous databases

GeneWikii LASS1.
GenomeRNAii 10715.
NextBioi 40679.
PROi P27544.
SOURCEi Search...

Gene expression databases

Bgeei P27544.
CleanExi HS_LASS1.
ExpressionAtlasi P27544. baseline and differential.
Genevestigatori P27544.

Family and domain databases

InterProi IPR016439. Longevity_assurance_LAG1_LAC1.
IPR006634. TLC-dom.
[Graphical view ]
Pfami PF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view ]
PIRSFi PIRSF005225. LAG1_LAC1. 1 hit.
SMARTi SM00724. TLC. 1 hit.
[Graphical view ]
PROSITEi PS50922. TLC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Expression of growth/differentiation factor 1 in the nervous system: conservation of a bicistronic structure."
    Lee S.-J.
    Proc. Natl. Acad. Sci. U.S.A. 88:4250-4254(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Homologs of the yeast longevity gene LAG1 in Caenorhabditis elegans and human."
    Jiang J.C., Kirchman P.A., Zagulski M., Hunt J., Jazwinski S.M.
    Genome Res. 8:1259-1272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1).
  3. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
    Tissue: Hypothalamus.
  5. "Comparative large-scale characterisation of plant vs. mammal proteins reveals similar and idiosyncratic N-alpha acetylation features."
    Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T., Giglione C.
    Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT ALA-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCERS1_HUMAN
AccessioniPrimary (citable) accession number: P27544
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

This protein is produced by a bicistronic gene which also produces the GDF1 protein from a non-overlapping reading frame.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3