P27543 (MTLD_ENTFA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Mannitol-1-phosphate 5-dehydrogenase
EC=1.1.1.17

Gene names

Name:	mtlD
Ordered Locus Names:	EF_0413

Organism

Enterococcus faecalis (Streptococcus faecalis)

Taxonomic identifier

1351 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Enterococcaceae › Enterococcus

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-mannitol 1-phosphate + NAD+ = D-fructose 6-phosphate + NADH. HAMAP MF_00196
Subunit structure	Monomer.
Sequence similarities	Belongs to the mannitol dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro mannitol-1-phosphate 5-dehydrogenase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 384	384	Mannitol-1-phosphate 5-dehydrogenase HAMAP MF_00196		PRO_0000170706
Regions
	Nucleotide binding	3 – 14	12	NAD By similarity
Experimental info
	Sequence conflict	23 – 28	6	NGFHIT → TGFILP in AAA24780. Ref.1
	Sequence conflict	34 – 37	4	ETII → GNHH in AAA24780. Ref.1
	Sequence conflict	115	1	K → NG in AAA24780. Ref.1
	Sequence conflict	138 – 146	9	KYLKNPAYA → IIFEKPSLS in AAA24780. Ref.1
	Sequence conflict	167	1	E → K in AAA24780. Ref.1
	Sequence conflict	198 – 202	5	HYVAD → ITCR in AAA24780. Ref.1
	Sequence conflict	215 – 216	2	NT → TS in AAA24780. Ref.1
	Sequence conflict	277	1	Q → N in AAA24780. Ref.1
	Sequence conflict	320 – 323	4	PHLL → AAFI in AAA24780. Ref.1
	Sequence conflict	356 – 384	29	IAEVT…RPQVA → DR in AAA24780. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P27543 [UniParc]. Last modified April 23, 2003. Version 2. Checksum: CE51664A668BC7CD	FASTA	384	43,099
	10 20 30 40 50 60 MNAVHFGAGN IGRGFIGEIL AKNGFHITFV DVNETIIQAL KERKSYTIEL ADASHQQINV 70 80 90 100 110 120 ENVTGLNNMT EPEKVVEAIA EADLVTTAIG PNILPRIAEL IAQGIDARAE ANCQKPLDII 130 140 150 160 170 180 ACENMIGGST FLAEEVAKYL KNPAYAEQWI GFPDAAVDRI VPLQKHEDPL FVQVEPFCEW 190 200 210 220 230 240 VIDDTNRKAK EIQLEGVHYV ADLEPYIERK LFSVNTGHAT VAYTGALLGY QTIDEAMQDA 250 260 270 280 290 300 LVVAQLKSVL QETGKLLVAK WNFDEQEHAA YIEKIIQRFQ NKYISDAITR VARTPIRKLG 310 320 330 340 350 360 AQERFIRPIR ELQERNLVSP HLLAMIGIVF NYHDPEDEQS RQLQEMLDQE SVDTVIAEVT 370 380 GIEDPETVKN IKQNVERYAR PQVA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Mannitol-specific phosphoenolpyruvate-dependent phosphotransferase system of Enterococcus faecalis: molecular cloning and nucleotide sequences of the enzyme IIIMtl gene and the mannitol-1-phosphate dehydrogenase gene, expression in Escherichia coli, and comparison of the gene products with similar enzymes." Fischer R., von Strandmann R.P., Hengstenberg W. J. Bacteriol. 173:3709-3715(1991) [PubMed: 1904856] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis." Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R., Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin S.A. , Kolonay J.F., Madupu R., Nelson W.C., Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M., Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M. Science 299:2071-2074(2003) [PubMed: 12663927] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700802 / V583.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38386 Genomic DNA. Translation: AAA24780.1. AE016830 Genomic DNA. Translation: AAO80272.1.
PIR	C39435.
RefSeq	NP_814201.1. NC_004668.1.
3D structure databases
ProteinModelPortal	P27543.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1199330.
GenomeReviews	Gene locus EF_0413 in contig AE016830_GR.
KEGG	efa:EF0413.
NMPDR	fig|226185.1.peg.387.
PATRIC	21851259. VBIEntFae7065_0382.
TIGR	EF_0413.
Phylogenomic databases
HOGENOM	HBG568825.
OMA	FANTAVD.
ProtClustDB	PRK02318.
Enzyme and pathway databases
BioCyc	EFAE226185:EF_0413-MONOMER.
Family and domain databases
HAMAP	MF_00196. Mannitol_dehydrog. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013328. DH_multihelical. IPR023028. Mannitol_1_phos_5_DH. IPR000669. Mannitol_DH. IPR013118. Mannitol_DH_C. IPR023027. Mannitol_DH_CS. IPR013131. Mannitol_DH_N. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00009.
Pfam	PF01232. Mannitol_dh. 1 hit. PF08125. Mannitol_dh_C. 1 hit. [Graphical view]
PRINTS	PR00084. MTLDHDRGNASE.
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
PROSITE	PS00974. MANNITOL_DHGENASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTLD_ENTFA

Accession

Primary (citable) accession number: P27543

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	April 23, 2003
Last modified:	January 25, 2012
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program