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Protein

Chlorophyll a-b binding protein 4, chloroplastic

Gene

LHCA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.1 Publication

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi57 – 571Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity
Binding sitei77 – 771Chlorophyll-a 1; via amide nitrogenBy similarity
Metal bindingi96 – 961Magnesium (chlorophyll-a 1 axial ligand)By similarity
Binding sitei101 – 1011Chlorophyll-b 2By similarity
Binding sitei138 – 1381Chlorophyll-b 3By similarity
Metal bindingi144 – 1441Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity
Metal bindingi154 – 1541Magnesium (chlorophyll-b 3 axial ligand)By similarity
Binding sitei157 – 1571Chlorophyll-b 4By similarity
Binding sitei204 – 2041Chlorophyll-a 5By similarity
Metal bindingi205 – 2051Magnesium (chlorophyll-a 3 axial ligand)By similarity
Metal bindingi208 – 2081Magnesium (chlorophyll-a 4 axial ligand)By similarity
Binding sitei210 – 2101Chlorophyll-a 1By similarity
Metal bindingi222 – 2221Magnesium (chlorophyll-a 5 axial ligand)By similarity
Metal bindingi237 – 2371Magnesium (chlorophyll-a 6 axial ligand)By similarity

GO - Molecular functioni

GO - Biological processi

  • photosynthesis, light harvesting in photosystem I Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • response to cold Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to karrikin Source: TAIR
  • response to light stimulus Source: GO_Central
  • response to low light intensity stimulus Source: UniProtKB
Complete GO annotation...

Keywords - Biological processi

Photosynthesis

Keywords - Ligandi

Chlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 4, chloroplastic1 Publication
Alternative name(s):
LHCI type III CAB-4
Gene namesi
Name:LHCA41 Publication
Synonyms:CAB4
Ordered Locus Names:At3g47470
ORF Names:F1P2.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G47470.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei102 – 12221HelicalSequence analysisAdd
BLAST
Transmembranei135 – 15521HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • chloroplast Source: TAIR
  • chloroplast envelope Source: TAIR
  • chloroplast thylakoid Source: TAIR
  • chloroplast thylakoid membrane Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: TAIR
  • photosystem I Source: UniProtKB-KW
  • plastoglobule Source: TAIR
  • thylakoid Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 251Chlorophyll a-b binding protein 4, chloroplasticPRO_0000003649
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei35 – 351PhosphoserineCombined sources

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27521.
PRIDEiP27521.

PTM databases

iPTMnetiP27521.

Expressioni

Inductioni

Induced by low light (LL) but repressed by high light (HL). Inhibited by cold.1 Publication

Gene expression databases

GenevisibleiP27521. AT.

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins. Red-emitting heterodimer with LHCA1 (PubMed:21083539).1 Publication

Protein-protein interaction databases

BioGridi9221. 4 interactions.
STRINGi3702.AT3G47470.1.

Structurei

3D structure databases

ProteinModelPortaliP27521.
SMRiP27521. Positions 54-249.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQCX. Eukaryota.
ENOG4110JH3. LUCA.
HOGENOMiHOG000238033.
InParanoidiP27521.
KOiK08910.
OMAiKEQYFAS.
PhylomeDBiP27521.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVTTHASA SIFRPCTSKP RFLTGSSGRL NRDLSFTSIG SSAKTSSFKV
60 70 80 90 100
EAKKGEWLPG LASPDYLTGS LAGDNGFDPL GLAEDPENLK WFVQAELVNG
110 120 130 140 150
RWAMLGVAGM LLPEVFTKIG IINVPEWYDA GKEQYFASSS TLFVIEFILF
160 170 180 190 200
HYVEIRRWQD IKNPGSVNQD PIFKQYSLPK GEVGYPGGIF NPLNFAPTQE
210 220 230 240 250
AKEKELANGR LAMLAFLGFV VQHNVTGKGP FENLLQHLSD PWHNTIVQTF

N
Length:251
Mass (Da):27,734
Last modified:August 1, 1992 - v1
Checksum:iDC8B390C2AED9D22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63931 mRNA. Translation: AAA32760.1.
AL132955 Genomic DNA. Translation: CAB61973.1.
CP002686 Genomic DNA. Translation: AEE78285.1.
AY093080 mRNA. Translation: AAM13079.1.
BT000093 mRNA. Translation: AAN15412.1.
AY086470 mRNA. Translation: AAM63472.1.
PIRiT45707.
RefSeqiNP_190331.3. NM_114615.3.
UniGeneiAt.21346.
At.27524.
At.35504.
At.35803.
At.66361.
At.68076.
At.71101.

Genome annotation databases

EnsemblPlantsiAT3G47470.1; AT3G47470.1; AT3G47470.
GeneIDi823901.
GrameneiAT3G47470.1; AT3G47470.1; AT3G47470.
KEGGiath:AT3G47470.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63931 mRNA. Translation: AAA32760.1.
AL132955 Genomic DNA. Translation: CAB61973.1.
CP002686 Genomic DNA. Translation: AEE78285.1.
AY093080 mRNA. Translation: AAM13079.1.
BT000093 mRNA. Translation: AAN15412.1.
AY086470 mRNA. Translation: AAM63472.1.
PIRiT45707.
RefSeqiNP_190331.3. NM_114615.3.
UniGeneiAt.21346.
At.27524.
At.35504.
At.35803.
At.66361.
At.68076.
At.71101.

3D structure databases

ProteinModelPortaliP27521.
SMRiP27521. Positions 54-249.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi9221. 4 interactions.
STRINGi3702.AT3G47470.1.

PTM databases

iPTMnetiP27521.

Proteomic databases

PaxDbiP27521.
PRIDEiP27521.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT3G47470.1; AT3G47470.1; AT3G47470.
GeneIDi823901.
GrameneiAT3G47470.1; AT3G47470.1; AT3G47470.
KEGGiath:AT3G47470.

Organism-specific databases

TAIRiAT3G47470.

Phylogenomic databases

eggNOGiENOG410IQCX. Eukaryota.
ENOG4110JH3. LUCA.
HOGENOMiHOG000238033.
InParanoidiP27521.
KOiK08910.
OMAiKEQYFAS.
PhylomeDBiP27521.

Miscellaneous databases

PROiP27521.

Gene expression databases

GenevisibleiP27521. AT.

Family and domain databases

Gene3Di1.10.3460.10. 1 hit.
InterProiIPR001344. Chloro_AB-bd_pln.
IPR022796. Chloroa_b-bind.
IPR023329. Chlorophyll_a/b-bd_dom.
[Graphical view]
PANTHERiPTHR21649. PTHR21649. 1 hit.
PfamiPF00504. Chloroa_b-bind. 1 hit.
[Graphical view]
SUPFAMiSSF103511. SSF103511. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation, characterization and chromosomal location of a new cab gene from Arabidopsis thaliana."
    Zhang H., Hanley S., Goodman H.M.
    Plant Physiol. 96:1387-1388(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
    Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F.
    , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
    Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
  6. "A guide to the Lhc genes and their relatives in Arabidopsis."
    Jansson S.
    Trends Plant Sci. 4:236-240(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  7. "The PSI-K subunit of photosystem I is involved in the interaction between light-harvesting complex I and the photosystem I reaction center core."
    Jensen P.E., Gilpin M., Knoetzel J., Scheller H.V.
    J. Biol. Chem. 275:24701-24708(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
    Strain: cv. Columbia.
  8. "Pigment organization and energy transfer dynamics in isolated photosystem I (PSI) complexes from Arabidopsis thaliana depleted of the PSI-G, PSI-K, PSI-L, or PSI-N subunit."
    Ihalainen J.A., Jensen P.E., Haldrup A., van Stokkum I.H.M., van Grondelle R., Scheller H.V., Dekker J.P.
    Biophys. J. 83:2190-2201(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON PHOTOSYSTEM I ANTENNA.
  9. "Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana."
    Ferro M., Salvi D., Brugiere S., Miras S., Kowalski S., Louwagie M., Garin J., Joyard J., Rolland N.
    Mol. Cell. Proteomics 2:325-345(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Strain: cv. Wassilewskija.
  10. "Lhca5--an LHC-type protein associated with photosystem I."
    Ganeteg U., Klimmek F., Jansson S.
    Plant Mol. Biol. 54:641-651(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION BY LIGHT AND COLD.
    Strain: cv. C24 and cv. Columbia.
  11. "Pigment binding, fluorescence properties, and oligomerization behavior of Lhca5, a novel light-harvesting protein."
    Storf S., Jansson S., Schmid V.H.R.
    J. Biol. Chem. 280:5163-5168(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: COFACTOR.
  12. "Large-scale Arabidopsis phosphoproteome profiling reveals novel chloroplast kinase substrates and phosphorylation networks."
    Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A., Grossmann J., Gruissem W., Baginsky S.
    Plant Physiol. 150:889-903(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-35, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "The light-harvesting complexes of higher-plant Photosystem I: Lhca1/4 and Lhca2/3 form two red-emitting heterodimers."
    Wientjes E., Croce R.
    Biochem. J. 433:477-485(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, COFACTOR.
    Strain: cv. Columbia.
  14. "The role of the individual Lhcas in photosystem I excitation energy trapping."
    Wientjes E., van Stokkum I.H.M., van Amerongen H., Croce R.
    Biophys. J. 101:745-754(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MISCELLANEOUS.

Entry informationi

Entry nameiCA4_ARATH
AccessioniPrimary (citable) accession number: P27521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 13, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Light emission at 715-720 nm upon excitation at 440 and 475 nm, and subsequent transfer of excitation energy to the photosystem I core with a relative slow rate of 25 ns(-1).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.