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Protein

Chlorophyll a-b binding protein 4, chloroplastic

Gene

LHCA4

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The light-harvesting complex (LHC) functions as a light receptor, it captures and delivers excitation energy to photosystems with which it is closely associated.1 Publication

Miscellaneous

Light emission at 715-720 nm upon excitation at 440 and 475 nm, and subsequent transfer of excitation energy to the photosystem I core with a relative slow rate of 25 ns(-1).1 Publication

Cofactori

Note: Binds at least 14 chlorophylls (8 Chl-a and 6 Chl-b) and carotenoids such as lutein and neoxanthin.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi57Magnesium (chlorophyll-b 1 axial ligand); via carbonyl oxygenBy similarity1
Binding sitei77Chlorophyll-a 1; via amide nitrogenBy similarity1
Metal bindingi96Magnesium (chlorophyll-a 1 axial ligand)By similarity1
Binding sitei101Chlorophyll-b 2By similarity1
Binding sitei138Chlorophyll-b 3By similarity1
Metal bindingi144Magnesium (chlorophyll-b 2 axial ligand); via carbonyl oxygenBy similarity1
Metal bindingi154Magnesium (chlorophyll-b 3 axial ligand)By similarity1
Binding sitei157Chlorophyll-b 4By similarity1
Binding sitei204Chlorophyll-a 5By similarity1
Metal bindingi205Magnesium (chlorophyll-a 3 axial ligand)By similarity1
Metal bindingi208Magnesium (chlorophyll-a 4 axial ligand)By similarity1
Binding sitei210Chlorophyll-a 1By similarity1
Metal bindingi222Magnesium (chlorophyll-a 5 axial ligand)By similarity1
Metal bindingi237Magnesium (chlorophyll-a 6 axial ligand)By similarity1

GO - Molecular functioni

  • chlorophyll binding Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • pigment binding Source: GO_Central
  • protein domain specific binding Source: CAFA

GO - Biological processi

  • photosynthesis, light harvesting in photosystem I Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
  • response to cold Source: UniProtKB
  • response to high light intensity Source: UniProtKB
  • response to karrikin Source: TAIR
  • response to low light intensity stimulus Source: UniProtKB

Keywordsi

Biological processPhotosynthesis
LigandChlorophyll, Chromophore, Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chlorophyll a-b binding protein 4, chloroplastic1 Publication
Alternative name(s):
LHCI type III CAB-4
Gene namesi
Name:LHCA41 Publication
Synonyms:CAB4
Ordered Locus Names:At3g47470
ORF Names:F1P2.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G47470
TAIRilocus:2079117 AT3G47470

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei102 – 122HelicalSequence analysisAdd BLAST21
Transmembranei135 – 155HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Chloroplast, Membrane, Photosystem I, Plastid, Thylakoid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000003649? – 251Chlorophyll a-b binding protein 4, chloroplastic
Transit peptidei1 – ?ChloroplastSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35PhosphoserineCombined sources1

Post-translational modificationi

Photoregulated by reversible phosphorylation of its threonine residues.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP27521
PRIDEiP27521

PTM databases

iPTMnetiP27521

Expressioni

Inductioni

Induced by low light (LL) but repressed by high light (HL). Inhibited by cold.1 Publication

Gene expression databases

ExpressionAtlasiP27521 baseline and differential
GenevisibleiP27521 AT

Interactioni

Subunit structurei

The LHC complex consists of chlorophyll a-b binding proteins. Red-emitting heterodimer with LHCA1 (PubMed:21083539).1 Publication

GO - Molecular functioni

  • protein domain specific binding Source: CAFA

Protein-protein interaction databases

BioGridi9221, 4 interactors
STRINGi3702.AT3G47470.1

Structurei

3D structure databases

ProteinModelPortaliP27521
SMRiP27521
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

The N-terminus of the protein extends into the stroma where it is involved with adhesion of granal membranes and post-translational modifications; both are believed to mediate the distribution of excitation energy between photosystems I and II.

Sequence similaritiesi

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IQCX Eukaryota
ENOG4110JH3 LUCA
HOGENOMiHOG000238033
InParanoidiP27521
KOiK08910
OMAiILNVPKW
OrthoDBiEOG09360J56
PhylomeDBiP27521

Family and domain databases

Gene3Di1.10.3460.10, 1 hit
InterProiView protein in InterPro
IPR001344 Chloro_AB-bd_pln
IPR022796 Chloroa_b-bind
IPR023329 Chlorophyll_a/b-bd_dom_sf
PANTHERiPTHR21649 PTHR21649, 1 hit
PfamiView protein in Pfam
PF00504 Chloroa_b-bind, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P27521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATVTTHASA SIFRPCTSKP RFLTGSSGRL NRDLSFTSIG SSAKTSSFKV
60 70 80 90 100
EAKKGEWLPG LASPDYLTGS LAGDNGFDPL GLAEDPENLK WFVQAELVNG
110 120 130 140 150
RWAMLGVAGM LLPEVFTKIG IINVPEWYDA GKEQYFASSS TLFVIEFILF
160 170 180 190 200
HYVEIRRWQD IKNPGSVNQD PIFKQYSLPK GEVGYPGGIF NPLNFAPTQE
210 220 230 240 250
AKEKELANGR LAMLAFLGFV VQHNVTGKGP FENLLQHLSD PWHNTIVQTF

N
Length:251
Mass (Da):27,734
Last modified:August 1, 1992 - v1
Checksum:iDC8B390C2AED9D22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M63931 mRNA Translation: AAA32760.1
AL132955 Genomic DNA Translation: CAB61973.1
CP002686 Genomic DNA Translation: AEE78285.1
AY093080 mRNA Translation: AAM13079.1
BT000093 mRNA Translation: AAN15412.1
AY086470 mRNA Translation: AAM63472.1
PIRiT45707
RefSeqiNP_190331.3, NM_114615.4
UniGeneiAt.21346
At.27524
At.35504
At.35803
At.66361
At.68076
At.71101

Genome annotation databases

EnsemblPlantsiAT3G47470.1; AT3G47470.1; AT3G47470
GeneIDi823901
GrameneiAT3G47470.1; AT3G47470.1; AT3G47470
KEGGiath:AT3G47470

Similar proteinsi

Entry informationi

Entry nameiCA4_ARATH
AccessioniPrimary (citable) accession number: P27521
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 25, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health