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P27515 (URK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 123. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Uridine kinase

EC=2.7.1.48
Alternative name(s):
Uridine monophosphokinase
Gene names
Name:URK1
Ordered Locus Names:YNR012W
ORF Names:N2050
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length501 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway. Ref.1 Ref.5

Catalytic activity

ATP + uridine = ADP + UMP.

ATP + cytidine = ADP + CMP.

Pathway

Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3.

Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.

Subcellular location

Cytoplasm. Nucleus Ref.6.

Sequence similarities

Belongs to the uridine kinase family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

DAS2Q120845EBI-20151,EBI-35824
FUR1P185624EBI-20151,EBI-20122

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 501501Uridine kinase
PRO_0000164462

Regions

Nucleotide binding63 – 708ATP Potential

Amino acid modifications

Modified residue171Phosphoserine Ref.8 Ref.10
Modified residue2761Phosphoserine Ref.10

Sequences

Sequence LengthMass (Da)Tools
P27515 [UniParc].

Last modified August 1, 1992. Version 1.
Checksum: 30A0592899BCD040

FASTA50156,296
        10         20         30         40         50         60 
MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI PPWTTPYIIG 

        70         80         90        100        110        120 
IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE DRARAFKNEY DFDEPNAINL 

       130        140        150        160        170        180 
DLAYKCILNL KEGKRTNIPV YSFVHHNRVP DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD 

       190        200        210        220        230        240 
LKIYVDADLD VCLARRLSRD IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS 

       250        260        270        280        290        300 
MSDNATAVNL IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH 

       310        320        330        340        350        360 
TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV IACQFDQVTA 

       370        380        390        400        410        420 
VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH CEFLPPNIEK FGKVFLMEGQ 

       430        440        450        460        470        480 
IISGAAMIMA IQVLLDHGID LEKISVVVYL ATEVGIRRIL NAFDNKVNIF AGMIISREKL 

       490        500 
QNHQYKWALT RFFDSKYFGC D 

« Hide

References

« Hide 'large scale' references
[1]"The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase."
Kern L.
Nucleic Acids Res. 18:5279-5279(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
Verhasselt P., Aert R., Voet M., Volckaert G.
Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[3]"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. expand/collapse author list , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism."
Kurtz J.-E., Exinger F., Erbs P., Jund R.
Curr. Genet. 36:130-136(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: YAL6B.
[8]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[9]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X53998 Genomic DNA. Translation: CAA37946.1.
X77395 Genomic DNA. Translation: CAA54580.1.
Z71627 Genomic DNA. Translation: CAA96289.1.
BK006947 Genomic DNA. Translation: DAA10553.1.
PIRS29374.
RefSeqNP_014409.1. NM_001183189.1.

3D structure databases

ProteinModelPortalP27515.
SMRP27515. Positions 56-259, 279-499.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid35837. 21 interactions.
DIPDIP-1720N.
IntActP27515. 6 interactions.
MINTMINT-406983.
STRING4932.YNR012W.

Proteomic databases

PaxDbP27515.
PeptideAtlasP27515.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYNR012W; YNR012W; YNR012W.
GeneID855746.
KEGGsce:YNR012W.

Organism-specific databases

CYGDYNR012w.
SGDS000005295. URK1.

Phylogenomic databases

eggNOGCOG0572.
GeneTreeENSGT00390000015696.
HOGENOMHOG000262757.
KOK00876.
OMAHVILMDS.
OrthoDBEOG7BKD5H.

Enzyme and pathway databases

BioCycMetaCyc:YNR012W-MONOMER.
YEAST:YNR012W-MONOMER.
UniPathwayUPA00574; UER00637.
UPA00579; UER00640.

Gene expression databases

GenevestigatorP27515.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR000764. Uridine_kinase.
[Graphical view]
PfamPF00485. PRK. 1 hit.
[Graphical view]
PRINTSPR00988. URIDINKINASE.
SUPFAMSSF52540. SSF52540. 1 hit.
TIGRFAMsTIGR00235. udk. 1 hit.
ProtoNetSearch...

Other

NextBio980151.
PROP27515.

Entry information

Entry nameURK1_YEAST
AccessionPrimary (citable) accession number: P27515
Secondary accession number(s): D6W1I7
Entry history
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: April 16, 2014
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome XIV

Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways