P27515 (URK1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine kinase
EC=2.7.1.48

Alternative name(s):
Uridine monophosphokinase

Gene names

Name:	URK1
Ordered Locus Names:	YNR012W
ORF Names:	N2050

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway. Ref.1 Ref.5
Catalytic activity	ATP + uridine = ADP + UMP. ATP + cytidine = ADP + CMP.
Pathway	Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Subcellular location	Cytoplasm. Nucleus Ref.6.
Sequence similarities	Belongs to the uridine kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	CTP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway UMP salvage Inferred from electronic annotation. Source: UniProtKB-UniPathway pyrimidine-containing compound salvage Inferred from genetic interaction PubMed 11872485. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: SGD nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro uridine kinase activity Inferred from mutant phenotype Ref.5. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
DAS2	Q12084	5	EBI-20151,EBI-35824
FUR1	P18562	4	EBI-20151,EBI-20122

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Uridine kinase		PRO_0000164462
Regions
	Nucleotide binding	63 – 70	8	ATP Potential
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.7 Ref.9 Ref.10
	Modified residue	17	1	Phosphoserine Ref.8
	Modified residue	276	1	Phosphoserine Ref.10
	Modified residue	278	1	Phosphoserine Ref.10

Sequences

	Sequence		Length	Mass (Da)
	P27515 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 30A0592899BCD040	FASTA	501	56,296
	10 20 30 40 50 60 MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI PPWTTPYIIG 70 80 90 100 110 120 IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE DRARAFKNEY DFDEPNAINL 130 140 150 160 170 180 DLAYKCILNL KEGKRTNIPV YSFVHHNRVP DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD 190 200 210 220 230 240 LKIYVDADLD VCLARRLSRD IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS 250 260 270 280 290 300 MSDNATAVNL IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH 310 320 330 340 350 360 TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV IACQFDQVTA 370 380 390 400 410 420 VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH CEFLPPNIEK FGKVFLMEGQ 430 440 450 460 470 480 IISGAAMIMA IQVLLDHGID LEKISVVVYL ATEVGIRRIL NAFDNKVNIF AGMIISREKL 490 500 QNHQYKWALT RFFDSKYFGC D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase." Kern L. Nucleic Acids Res. 18:5279-5279(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm." Verhasselt P., Aert R., Voet M., Volckaert G. Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism." Kurtz J.-E., Exinger F., Erbs P., Jund R. Curr. Genet. 36:130-136(1999) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY. Strain: ADR376.
[9]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
[10]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-276 AND SER-278, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X53998 Genomic DNA. Translation: CAA37946.1. X77395 Genomic DNA. Translation: CAA54580.1. Z71627 Genomic DNA. Translation: CAA96289.1. BK006947 Genomic DNA. Translation: DAA10553.1.
PIR	S29374.
RefSeq	NP_014409.1. NM_001183189.1.
3D structure databases
ProteinModelPortal	P27515.
SMR	P27515. Positions 56-259, 279-499.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1720N.
IntAct	P27515. 6 interactions.
MINT	MINT-406983.
STRING	4932.YNR012W.
Proteomic databases
PaxDb	P27515.
PeptideAtlas	P27515.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YNR012W; YNR012W; YNR012W.
GeneID	855746.
KEGG	sce:YNR012W.
Organism-specific databases
CYGD	YNR012w.
SGD	S000005295. URK1.
Phylogenomic databases
eggNOG	COG0572.
GeneTree	ENSGT00390000015696.
HOGENOM	HOG000262757.
KO	K00876.
OMA	RIQRDTV.
OrthoDB	EOG4NZZ30.
Enzyme and pathway databases
UniPathway	UPA00574; UER00637. UPA00579; UER00640.
Gene expression databases
Genevestigator	P27515.
GermOnline	YNR012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006083. PRK/URK. IPR000764. Uridine_kinase. [Graphical view]
Pfam	PF00485. PRK. 1 hit. [Graphical view]
PRINTS	PR00988. URIDINKINASE.
TIGRFAMs	TIGR00235. udk. 1 hit.
ProtoNet	Search...
Other
NextBio	980151.

Entry information

Entry name

URK1_YEAST

Accession

Primary (citable) accession number: P27515
Secondary accession number(s): D6W1I7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	May 1, 2013
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program