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Protein

Uridine kinase

Gene

URK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway.2 Publications

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi: CTP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes CTP from cytidine.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
This subpathway is part of the pathway CTP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CTP from cytidine, the pathway CTP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Pathwayi: UMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes UMP from uridine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative uridine kinase DAS2 (DAS2), Uridine kinase (URK1)
This subpathway is part of the pathway UMP biosynthesis via salvage pathway, which is itself part of Pyrimidine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes UMP from uridine, the pathway UMP biosynthesis via salvage pathway and in Pyrimidine metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi63 – 70ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • uridine kinase activity Source: SGD

GO - Biological processi

  • CTP salvage Source: UniProtKB-UniPathway
  • pyrimidine-containing compound salvage Source: SGD
  • pyrimidine nucleobase metabolic process Source: GO_Central
  • pyrimidine nucleoside salvage Source: GO_Central
  • UMP salvage Source: UniProtKB-UniPathway

Keywordsi

Molecular functionKinase, Transferase
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YNR012W-MONOMER.
YEAST:YNR012W-MONOMER.
ReactomeiR-SCE-73614. Pyrimidine salvage.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine kinase (EC:2.7.1.48)
Alternative name(s):
Uridine monophosphokinase
Gene namesi
Name:URK1
Ordered Locus Names:YNR012W
ORF Names:N2050
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR012W.
SGDiS000005295. URK1.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001644621 – 501Uridine kinaseAdd BLAST501

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1
Modified residuei276PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27515.
PRIDEiP27515.

PTM databases

iPTMnetiP27515.

Interactioni

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi35837. 80 interactors.
DIPiDIP-1720N.
IntActiP27515. 7 interactors.
MINTiMINT-406983.
STRINGi4932.YNR012W.

Structurei

3D structure databases

ProteinModelPortaliP27515.
SMRiP27515.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262757.
InParanoidiP27515.
KOiK00876.
OMAiCYAGKQI.
OrthoDBiEOG092C4GPE.

Family and domain databases

CDDicd06223. PRTases_typeI. 1 hit.
cd02023. UMPK. 1 hit.
InterProiView protein in InterPro
IPR027417. P-loop_NTPase.
IPR000836. PRibTrfase_dom.
IPR006083. PRK/URK.
IPR029057. PRTase-like.
IPR000764. Uridine_kinase-like.
PfamiView protein in Pfam
PF00485. PRK. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

P27515-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI
60 70 80 90 100
PPWTTPYIIG IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE
110 120 130 140 150
DRARAFKNEY DFDEPNAINL DLAYKCILNL KEGKRTNIPV YSFVHHNRVP
160 170 180 190 200
DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD LKIYVDADLD VCLARRLSRD
210 220 230 240 250
IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS MSDNATAVNL
260 270 280 290 300
IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH
310 320 330 340 350
TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV
360 370 380 390 400
IACQFDQVTA VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH
410 420 430 440 450
CEFLPPNIEK FGKVFLMEGQ IISGAAMIMA IQVLLDHGID LEKISVVVYL
460 470 480 490 500
ATEVGIRRIL NAFDNKVNIF AGMIISREKL QNHQYKWALT RFFDSKYFGC

D
Length:501
Mass (Da):56,296
Last modified:August 1, 1992 - v1
Checksum:i30A0592899BCD040
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X53998 Genomic DNA. Translation: CAA37946.1.
X77395 Genomic DNA. Translation: CAA54580.1.
Z71627 Genomic DNA. Translation: CAA96289.1.
BK006947 Genomic DNA. Translation: DAA10553.1.
PIRiS29374.
RefSeqiNP_014409.1. NM_001183189.1.

Genome annotation databases

EnsemblFungiiYNR012W; YNR012W; YNR012W.
GeneIDi855746.
KEGGisce:YNR012W.

Similar proteinsi

Entry informationi

Entry nameiURK1_YEAST
AccessioniPrimary (citable) accession number: P27515
Secondary accession number(s): D6W1I7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 25, 2017
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names