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P27515

- URK1_YEAST

UniProt

P27515 - URK1_YEAST

Protein

Uridine kinase

Gene

URK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Aug 1992)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway.2 Publications

    Catalytic activityi

    ATP + uridine = ADP + UMP.
    ATP + cytidine = ADP + CMP.

    Pathwayi

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi63 – 708ATPSequence Analysis

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein binding Source: IntAct
    3. uridine kinase activity Source: SGD

    GO - Biological processi

    1. CTP salvage Source: UniProtKB-UniPathway
    2. pyrimidine-containing compound salvage Source: SGD
    3. UMP salvage Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciMetaCyc:YNR012W-MONOMER.
    YEAST:YNR012W-MONOMER.
    UniPathwayiUPA00574; UER00637.
    UPA00579; UER00640.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uridine kinase (EC:2.7.1.48)
    Alternative name(s):
    Uridine monophosphokinase
    Gene namesi
    Name:URK1
    Ordered Locus Names:YNR012W
    ORF Names:N2050
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome XIV

    Organism-specific databases

    CYGDiYNR012w.
    SGDiS000005295. URK1.

    Subcellular locationi

    Cytoplasm 1 Publication. Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 501501Uridine kinasePRO_0000164462Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei17 – 171Phosphoserine2 Publications
    Modified residuei276 – 2761Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP27515.
    PaxDbiP27515.
    PeptideAtlasiP27515.

    Expressioni

    Gene expression databases

    GenevestigatoriP27515.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    DAS2Q120845EBI-20151,EBI-35824
    FUR1P185624EBI-20151,EBI-20122

    Protein-protein interaction databases

    BioGridi35837. 21 interactions.
    DIPiDIP-1720N.
    IntActiP27515. 6 interactions.
    MINTiMINT-406983.
    STRINGi4932.YNR012W.

    Structurei

    3D structure databases

    ProteinModelPortaliP27515.
    SMRiP27515. Positions 56-259, 279-499.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the uridine kinase family.Curated

    Phylogenomic databases

    eggNOGiCOG0572.
    GeneTreeiENSGT00390000015696.
    HOGENOMiHOG000262757.
    KOiK00876.
    OMAiEGLNDQF.
    OrthoDBiEOG7BKD5H.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR006083. PRK/URK.
    IPR029057. PRTase-like.
    IPR000764. Uridine_kinase_like.
    [Graphical view]
    PfamiPF00485. PRK. 1 hit.
    [Graphical view]
    PRINTSiPR00988. URIDINKINASE.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR00235. udk. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P27515-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI    50
    PPWTTPYIIG IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE 100
    DRARAFKNEY DFDEPNAINL DLAYKCILNL KEGKRTNIPV YSFVHHNRVP 150
    DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD LKIYVDADLD VCLARRLSRD 200
    IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS MSDNATAVNL 250
    IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH 300
    TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV 350
    IACQFDQVTA VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH 400
    CEFLPPNIEK FGKVFLMEGQ IISGAAMIMA IQVLLDHGID LEKISVVVYL 450
    ATEVGIRRIL NAFDNKVNIF AGMIISREKL QNHQYKWALT RFFDSKYFGC 500
    D 501
    Length:501
    Mass (Da):56,296
    Last modified:August 1, 1992 - v1
    Checksum:i30A0592899BCD040
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53998 Genomic DNA. Translation: CAA37946.1.
    X77395 Genomic DNA. Translation: CAA54580.1.
    Z71627 Genomic DNA. Translation: CAA96289.1.
    BK006947 Genomic DNA. Translation: DAA10553.1.
    PIRiS29374.
    RefSeqiNP_014409.1. NM_001183189.1.

    Genome annotation databases

    EnsemblFungiiYNR012W; YNR012W; YNR012W.
    GeneIDi855746.
    KEGGisce:YNR012W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X53998 Genomic DNA. Translation: CAA37946.1 .
    X77395 Genomic DNA. Translation: CAA54580.1 .
    Z71627 Genomic DNA. Translation: CAA96289.1 .
    BK006947 Genomic DNA. Translation: DAA10553.1 .
    PIRi S29374.
    RefSeqi NP_014409.1. NM_001183189.1.

    3D structure databases

    ProteinModelPortali P27515.
    SMRi P27515. Positions 56-259, 279-499.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 35837. 21 interactions.
    DIPi DIP-1720N.
    IntActi P27515. 6 interactions.
    MINTi MINT-406983.
    STRINGi 4932.YNR012W.

    Proteomic databases

    MaxQBi P27515.
    PaxDbi P27515.
    PeptideAtlasi P27515.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YNR012W ; YNR012W ; YNR012W .
    GeneIDi 855746.
    KEGGi sce:YNR012W.

    Organism-specific databases

    CYGDi YNR012w.
    SGDi S000005295. URK1.

    Phylogenomic databases

    eggNOGi COG0572.
    GeneTreei ENSGT00390000015696.
    HOGENOMi HOG000262757.
    KOi K00876.
    OMAi EGLNDQF.
    OrthoDBi EOG7BKD5H.

    Enzyme and pathway databases

    UniPathwayi UPA00574 ; UER00637 .
    UPA00579 ; UER00640 .
    BioCyci MetaCyc:YNR012W-MONOMER.
    YEAST:YNR012W-MONOMER.

    Miscellaneous databases

    NextBioi 980151.
    PROi P27515.

    Gene expression databases

    Genevestigatori P27515.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR006083. PRK/URK.
    IPR029057. PRTase-like.
    IPR000764. Uridine_kinase_like.
    [Graphical view ]
    Pfami PF00485. PRK. 1 hit.
    [Graphical view ]
    PRINTSi PR00988. URIDINKINASE.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR00235. udk. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase."
      Kern L.
      Nucleic Acids Res. 18:5279-5279(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
      Strain: ATCC 28383 / FL100 / VTT C-80102.
    2. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
      Verhasselt P., Aert R., Voet M., Volckaert G.
      Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 96604 / S288c / FY1679.
    3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
      Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
      , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
      Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism."
      Kurtz J.-E., Exinger F., Erbs P., Jund R.
      Curr. Genet. 36:130-136(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
      Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
      Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: YAL6B.
    8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiURK1_YEAST
    AccessioniPrimary (citable) accession number: P27515
    Secondary accession number(s): D6W1I7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 1, 1992
    Last sequence update: August 1, 1992
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome XIV
      Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

    External Data

    Dasty 3