Reviewed, UniProtKB/Swiss-Prot P27515 (URK1_YEAST)

Names and origin

Protein names

Recommended name:
    Uridine kinase
    EC=2.7.1.48
Alternative name(s):
    Uridine monophosphokinase

Gene names

Name:	URK1
Ordered Locus Names:	YNR012W
ORF Names:	N2050

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	501 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway. Ref.1 Ref.4
Catalytic activity	ATP + uridine = ADP + UMP. ATP + cytidine = ADP + CMP.
Pathway	Pyrimidine metabolism; CTP biosynthesis via salvage pathway; CTP from cytidine: step 1/3. Pyrimidine metabolism; UMP biosynthesis via salvage pathway; UMP from uridine: step 1/1.
Subcellular location	Cytoplasm. Nucleus. Ref.5
Sequence similarities	Belongs to the uridine kinase family.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pyrimidine salvage Inferred from genetic interaction. Source: SGD
Cellular component	cytoplasm Ref.5 Inferred from direct assay. Source: SGD nucleus Ref.5 Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW identical protein binding Inferred from physical interaction. Source: IntAct phosphotransferase activity, alcohol group as acceptor Inferred from electronic annotation. Source: InterPro uridine kinase activity Ref.4 Inferred from mutant phenotype. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-20151,EBI-20151
	Q12084	1	EBI-20151,EBI-35824
FUR1	P18562	1	EBI-20151,EBI-20122

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 501	501	Uridine kinase		PRO_0000164462
Regions
	Nucleotide binding	63 – 70	8	ATP Potential
Amino acid modifications
	Modified residue	14	1	Phosphoserine Ref.6 Ref.8 Ref.9
	Modified residue	17	1	Phosphoserine Ref.7
	Modified residue	276	1	Phosphoserine Ref.9
	Modified residue	278	1	Phosphoserine Ref.9

Sequences

	Sequence		Length	Mass (Da)
	P27515-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: 30A0592899BCD040	FASTA	501	56,296
	10 20 30 40 50 60 MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI PPWTTPYIIG 70 80 90 100 110 120 IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE DRARAFKNEY DFDEPNAINL 130 140 150 160 170 180 DLAYKCILNL KEGKRTNIPV YSFVHHNRVP DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD 190 200 210 220 230 240 LKIYVDADLD VCLARRLSRD IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS 250 260 270 280 290 300 MSDNATAVNL IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH 310 320 330 340 350 360 TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV IACQFDQVTA 370 380 390 400 410 420 VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH CEFLPPNIEK FGKVFLMEGQ 430 440 450 460 470 480 IISGAAMIMA IQVLLDHGID LEKISVVVYL ATEVGIRRIL NAFDNKVNIF AGMIISREKL 490 500 QNHQYKWALT RFFDSKYFGC D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase." Kern L. Nucleic Acids Res. 18:5279-5279(1990) [PubMed: 2169608] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION. Strain: ATCC 28383 / FL100 / VTT C-80102.
[2]	"Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm." Verhasselt P., Aert R., Voet M., Volckaert G. Yeast 10:1355-1361(1994) [PubMed: 7900425] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications." Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J. , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J. Nature 387:93-98(1997) [PubMed: 9169873] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	"New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism." Kurtz J.-E., Exinger F., Erbs P., Jund R. Curr. Genet. 36:130-136(1999) [PubMed: 10501935] [Abstract] Cited for: FUNCTION.
[5]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-14; SER-276 AND SER-278, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X53998 Genomic DNA. Translation: CAA37946.1. X77395 Genomic DNA. Translation: CAA54580.1. Z71627 Genomic DNA. Translation: CAA96289.1.
PIR	S29374.
RefSeq	NP_014409.1.
3D structure databases
HSSP	HSSP built from PDB template 1BD3 based on UniProtKB Q26998.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:1720N.
IntAct	P27515. 11 interactions.
Proteomic databases
PeptideAtlas	P27515.
Genome annotation databases
Ensembl	YNR012W. Saccharomyces cerevisiae. [Contig view]
GeneID	855746.
GenomeReviews	Gene locus YNR012W in contig Y13139_GR.
KEGG	sce:YNR012W.
NMPDR	fig|4932.3.peg.5489.
Organism-specific databases
CYGD	YNR012w.
SGD	S000005295. URK1.
Yeast-GFP	Search...
Phylogenomic databases
HOGENOM	P27515.
OMA	P27515. CEDRSNA.
Enzyme and pathway databases
BRENDA	2.7.1.48. 250.
Gene expression databases
ArrayExpress	P27515.
GermOnline	YNR012W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR006083. PRK_URK. IPR000764. Uridine_kinase. [Graphical view]
PANTHER	PTHR10285:SF6. Uridine_kin. 1 hit.
Pfam	PF00485. PRK. 1 hit. [Graphical view]
PRINTS	PR00988. URIDINKINASE.
TIGRFAMs	TIGR00235. udk. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	980151.

Entry information

Entry name

URK1_YEAST

Accession

Primary (citable) accession number: P27515

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)