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P27515

- URK1_YEAST

UniProt

P27515 - URK1_YEAST

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Protein

Uridine kinase

Gene

URK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the conversion of uridine into UMP and cytidine into CMP in the pyrimidine salvage pathway.2 Publications

Catalytic activityi

ATP + uridine = ADP + UMP.
ATP + cytidine = ADP + CMP.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi63 – 708ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. uridine kinase activity Source: SGD

GO - Biological processi

  1. CTP salvage Source: UniProtKB-UniPathway
  2. pyrimidine-containing compound salvage Source: SGD
  3. UMP salvage Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMetaCyc:YNR012W-MONOMER.
YEAST:YNR012W-MONOMER.
UniPathwayiUPA00574; UER00637.
UPA00579; UER00640.

Names & Taxonomyi

Protein namesi
Recommended name:
Uridine kinase (EC:2.7.1.48)
Alternative name(s):
Uridine monophosphokinase
Gene namesi
Name:URK1
Ordered Locus Names:YNR012W
ORF Names:N2050
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XIV

Organism-specific databases

CYGDiYNR012w.
SGDiS000005295. URK1.

Subcellular locationi

Cytoplasm 1 Publication. Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 501501Uridine kinasePRO_0000164462Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171Phosphoserine2 Publications
Modified residuei276 – 2761Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP27515.
PaxDbiP27515.
PeptideAtlasiP27515.

Expressioni

Gene expression databases

GenevestigatoriP27515.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DAS2Q120845EBI-20151,EBI-35824
FUR1P185624EBI-20151,EBI-20122

Protein-protein interaction databases

BioGridi35837. 22 interactions.
DIPiDIP-1720N.
IntActiP27515. 6 interactions.
MINTiMINT-406983.
STRINGi4932.YNR012W.

Structurei

3D structure databases

ProteinModelPortaliP27515.
SMRiP27515. Positions 56-259, 279-499.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the uridine kinase family.Curated

Phylogenomic databases

eggNOGiCOG0572.
GeneTreeiENSGT00390000015696.
HOGENOMiHOG000262757.
InParanoidiP27515.
KOiK00876.
OMAiEGLNDQF.
OrthoDBiEOG7BKD5H.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029057. PRTase-like.
IPR000764. Uridine_kinase_like.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00988. URIDINKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsiTIGR00235. udk. 1 hit.

Sequencei

Sequence statusi: Complete.

P27515-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSHRIAPSKE RSSSFISILD DETRDTLKAN AVMDGEVDVK KTKGKSSRYI
60 70 80 90 100
PPWTTPYIIG IGGASGSGKT SVAAKIVSSI NVPWTVLISL DNFYNPLGPE
110 120 130 140 150
DRARAFKNEY DFDEPNAINL DLAYKCILNL KEGKRTNIPV YSFVHHNRVP
160 170 180 190 200
DKNIVIYGAS VVVIEGIYAL YDRRLLDLMD LKIYVDADLD VCLARRLSRD
210 220 230 240 250
IVSRGRDLDG CIQQWEKFVK PNAVKFVKPT MKNADAIIPS MSDNATAVNL
260 270 280 290 300
IINHIKSKLE LKSNEHLREL IKLGSSPSQD VLNRNIIHEL PPTNQVLSLH
310 320 330 340 350
TMLLNKNLNC ADFVFYFDRL ATILLSWALD DIPVAHTNII TPGEHTMENV
360 370 380 390 400
IACQFDQVTA VNIIRSGDCF MKSLRKTIPN ITIGKLLIQS DSQTGEPQLH
410 420 430 440 450
CEFLPPNIEK FGKVFLMEGQ IISGAAMIMA IQVLLDHGID LEKISVVVYL
460 470 480 490 500
ATEVGIRRIL NAFDNKVNIF AGMIISREKL QNHQYKWALT RFFDSKYFGC

D
Length:501
Mass (Da):56,296
Last modified:August 1, 1992 - v1
Checksum:i30A0592899BCD040
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53998 Genomic DNA. Translation: CAA37946.1.
X77395 Genomic DNA. Translation: CAA54580.1.
Z71627 Genomic DNA. Translation: CAA96289.1.
BK006947 Genomic DNA. Translation: DAA10553.1.
PIRiS29374.
RefSeqiNP_014409.1. NM_001183189.1.

Genome annotation databases

EnsemblFungiiYNR012W; YNR012W; YNR012W.
GeneIDi855746.
KEGGisce:YNR012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X53998 Genomic DNA. Translation: CAA37946.1 .
X77395 Genomic DNA. Translation: CAA54580.1 .
Z71627 Genomic DNA. Translation: CAA96289.1 .
BK006947 Genomic DNA. Translation: DAA10553.1 .
PIRi S29374.
RefSeqi NP_014409.1. NM_001183189.1.

3D structure databases

ProteinModelPortali P27515.
SMRi P27515. Positions 56-259, 279-499.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 35837. 22 interactions.
DIPi DIP-1720N.
IntActi P27515. 6 interactions.
MINTi MINT-406983.
STRINGi 4932.YNR012W.

Proteomic databases

MaxQBi P27515.
PaxDbi P27515.
PeptideAtlasi P27515.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YNR012W ; YNR012W ; YNR012W .
GeneIDi 855746.
KEGGi sce:YNR012W.

Organism-specific databases

CYGDi YNR012w.
SGDi S000005295. URK1.

Phylogenomic databases

eggNOGi COG0572.
GeneTreei ENSGT00390000015696.
HOGENOMi HOG000262757.
InParanoidi P27515.
KOi K00876.
OMAi EGLNDQF.
OrthoDBi EOG7BKD5H.

Enzyme and pathway databases

UniPathwayi UPA00574 ; UER00637 .
UPA00579 ; UER00640 .
BioCyci MetaCyc:YNR012W-MONOMER.
YEAST:YNR012W-MONOMER.

Miscellaneous databases

NextBioi 980151.
PROi P27515.

Gene expression databases

Genevestigatori P27515.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR006083. PRK/URK.
IPR029057. PRTase-like.
IPR000764. Uridine_kinase_like.
[Graphical view ]
Pfami PF00485. PRK. 1 hit.
[Graphical view ]
PRINTSi PR00988. URIDINKINASE.
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF53271. SSF53271. 1 hit.
TIGRFAMsi TIGR00235. udk. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase."
    Kern L.
    Nucleic Acids Res. 18:5279-5279(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  2. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism."
    Kurtz J.-E., Exinger F., Erbs P., Jund R.
    Curr. Genet. 36:130-136(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-17 AND SER-276, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiURK1_YEAST
AccessioniPrimary (citable) accession number: P27515
Secondary accession number(s): D6W1I7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: August 1, 1992
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

External Data

Dasty 3