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Protein

Low-affinity phosphate transporter PHO91

Gene

PHO91

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Vacuolar phosphate transporter that probably exports phosphate from the vacuolar lumen to the cytosol.3 Publications

GO - Molecular functioni

  • glycerol-3-phosphate transmembrane transporter activity Source: SGD
  • inorganic phosphate transmembrane transporter activity Source: SGD

GO - Biological processi

  • glycerol-3-phosphate transport Source: SGD
  • phosphate ion transport Source: SGD
  • polyphosphate metabolic process Source: SGD
  • regulation of phosphate transmembrane transport Source: SGD
  • sodium ion transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Phosphate transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-33329-MONOMER.
BRENDAi3.6.3.27. 984.

Protein family/group databases

TCDBi2.A.47.2.2. the divalent anion:na(+) symporter (dass) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Low-affinity phosphate transporter PHO91
Gene namesi
Name:PHO91
Ordered Locus Names:YNR013C
ORF Names:N2052
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIV

Organism-specific databases

EuPathDBiFungiDB:YNR013C.
SGDiS000005296. PHO91.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei430 – 45021HelicalSequence analysisAdd
BLAST
Transmembranei474 – 49421HelicalSequence analysisAdd
BLAST
Transmembranei511 – 53121HelicalSequence analysisAdd
BLAST
Transmembranei557 – 57721HelicalSequence analysisAdd
BLAST
Transmembranei602 – 62221HelicalSequence analysisAdd
BLAST
Transmembranei642 – 66221HelicalSequence analysisAdd
BLAST
Transmembranei682 – 70221HelicalSequence analysisAdd
BLAST
Transmembranei706 – 72621HelicalSequence analysisAdd
BLAST
Transmembranei738 – 75821HelicalSequence analysisAdd
BLAST
Transmembranei777 – 79721HelicalSequence analysisAdd
BLAST
Transmembranei799 – 81921HelicalSequence analysisAdd
BLAST
Transmembranei824 – 84421HelicalSequence analysisAdd
BLAST
Transmembranei874 – 89421HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

  • fungal-type vacuole membrane Source: SGD
  • integral component of membrane Source: GO_Central
  • vacuole-mitochondrion membrane contact site Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 894894Low-affinity phosphate transporter PHO91PRO_0000172519Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei295 – 2951PhosphoserineCombined sources
Modified residuei311 – 3111PhosphoserineCombined sources
Modified residuei312 – 3121PhosphoserineCombined sources

Post-translational modificationi

Ubiquitinated by RSP5. RSP5-mediated ubiquitination initiates internalization and degradation by the endocytic pathway.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP27514.

PTM databases

iPTMnetiP27514.

Expressioni

Inductioni

Expression is constitutive and independent of inorganic phosphate concentration and PHO4 activity.1 Publication

Interactioni

Protein-protein interaction databases

BioGridi35838. 40 interactions.
DIPiDIP-4264N.
IntActiP27514. 9 interactions.
MINTiMINT-508894.

Structurei

3D structure databases

ProteinModelPortaliP27514.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 256256SPXPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 SPX domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00730000112809.
InParanoidiP27514.
KOiK14430.
OMAiACFASMW.
OrthoDBiEOG76MKHV.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
IPR004331. SPX_dom.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
PF03105. SPX. 2 hits.
[Graphical view]
PROSITEiPS51382. SPX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKFSHSLQFN SVPEWSTKYL AYSQLKKLIY SLQKDKLYSN NKHHVVEPHD
60 70 80 90 100
ANDENLPLLA DASPDDQFYI SKFVAALNQE LKKIDKFYIS QETGLIANYN
110 120 130 140 150
ELKDDVMELE NTNKATQLFN QQQQHQLQSV ARNRKSKSQQ RQRRFSSVSS
160 170 180 190 200
TDSNPSLTDM SIDSAPVIHT QVSNTTNNGN SMQNLASASV SLSNSNPVYL
210 220 230 240 250
SPFTQHRLSL KKRLISIYTQ LSELKDFIEL NQTGFSKICK KFDKSLNTNL
260 270 280 290 300
KQNYLNYIKF HSHVFNPATI NRIQHHITET ILTYASLNKG TRRPSNTFNL
310 320 330 340 350
DADRINNDEN SSGNEEDEDG NRQEVLDFQD AERELSSHLR DHVVWERNTV
360 370 380 390 400
WKDMMNLERK YQSAKTDNKK FSKLSSSQLR PNANITESMA MSSGGAGIIA
410 420 430 440 450
PSTDSLTFRE LMHLPPKQWL QFIMGQTSLL KFLLITSCFI ALLTFNLTPF
460 470 480 490 500
TQDSLQKNCF AILIYASLLW ATETIPLFVT SLMIPLLIVV FPVIKDPITS
510 520 530 540 550
QPMSPRDSSQ FILSTMWSSV IMLLLGGFTL AAALSKYNIA KVLSTHILAS
560 570 580 590 600
AGTNPHFILL TNMFVALFVS MWVSNVAAPV LCYSIVQPLL RTLPRNCSYA
610 620 630 640 650
KALILGIALA SNIGGMSSPI ASPQNIFSIG IMDPSPSWAE WFMIALPVCF
660 670 680 690 700
ICVMAIWVLL IITFPPEPNV KILQLHPSRD PFTLKQWFVT LVCIITIVLW
710 720 730 740 750
CLSNQISGIF GEMGIISIIP IVVFFGTGLL TSDDFNNFMW TIVVLAMGGT
760 770 780 790 800
TLGKAVSSSG LLSTMAQLIK AQVEHEPIFI IVLIFGLVIL VMATFVSHTV
810 820 830 840 850
AAMIIVPLMS EIGSNLPSGD HSRLLIVIAA LLCSSAMGLP TSGFPNVTAI
860 870 880 890
SMIDEVGDRY LTVGTFITRG VPASLLSYAA IVTVGYGILK VMGF
Length:894
Mass (Da):99,490
Last modified:February 1, 1995 - v2
Checksum:i21938C585EB05509
GO

Sequence cautioni

The sequence CAA37947.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77395 Genomic DNA. Translation: CAA54581.1.
Z71628 Genomic DNA. Translation: CAA96290.1.
Z71629 Genomic DNA. Translation: CAA96292.1.
X53998 Genomic DNA. Translation: CAA37947.1. Different initiation.
BK006947 Genomic DNA. Translation: DAA10554.1.
PIRiS45135.
RefSeqiNP_014410.3. NM_001183190.3.

Genome annotation databases

EnsemblFungiiYNR013C; YNR013C; YNR013C.
GeneIDi855747.
KEGGisce:YNR013C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X77395 Genomic DNA. Translation: CAA54581.1.
Z71628 Genomic DNA. Translation: CAA96290.1.
Z71629 Genomic DNA. Translation: CAA96292.1.
X53998 Genomic DNA. Translation: CAA37947.1. Different initiation.
BK006947 Genomic DNA. Translation: DAA10554.1.
PIRiS45135.
RefSeqiNP_014410.3. NM_001183190.3.

3D structure databases

ProteinModelPortaliP27514.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35838. 40 interactions.
DIPiDIP-4264N.
IntActiP27514. 9 interactions.
MINTiMINT-508894.

Protein family/group databases

TCDBi2.A.47.2.2. the divalent anion:na(+) symporter (dass) family.

PTM databases

iPTMnetiP27514.

Proteomic databases

MaxQBiP27514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYNR013C; YNR013C; YNR013C.
GeneIDi855747.
KEGGisce:YNR013C.

Organism-specific databases

EuPathDBiFungiDB:YNR013C.
SGDiS000005296. PHO91.

Phylogenomic databases

GeneTreeiENSGT00730000112809.
InParanoidiP27514.
KOiK14430.
OMAiACFASMW.
OrthoDBiEOG76MKHV.

Enzyme and pathway databases

BioCyciYEAST:G3O-33329-MONOMER.
BRENDAi3.6.3.27. 984.

Miscellaneous databases

PROiP27514.

Family and domain databases

InterProiIPR001898. Na/sul_symport.
IPR004331. SPX_dom.
[Graphical view]
PfamiPF00939. Na_sulph_symp. 1 hit.
PF03105. SPX. 2 hits.
[Graphical view]
PROSITEiPS51382. SPX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Twelve open reading frames revealed in the 23.6 kb segment flanking the centromere on the Saccharomyces cerevisiae chromosome XIV right arm."
    Verhasselt P., Aert R., Voet M., Volckaert G.
    Yeast 10:1355-1361(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  2. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIV and its evolutionary implications."
    Philippsen P., Kleine K., Poehlmann R., Duesterhoeft A., Hamberg K., Hegemann J.H., Obermaier B., Urrestarazu L.A., Aert R., Albermann K., Altmann R., Andre B., Baladron V., Ballesta J.P.G., Becam A.-M., Beinhauer J.D., Boskovic J., Buitrago M.J.
    , Bussereau F., Coster F., Crouzet M., D'Angelo M., Dal Pero F., De Antoni A., del Rey F., Doignon F., Domdey H., Dubois E., Fiedler T.A., Fleig U., Floeth M., Fritz C., Gaillardin C., Garcia-Cantalejo J.M., Glansdorff N., Goffeau A., Gueldener U., Herbert C.J., Heumann K., Heuss-Neitzel D., Hilbert H., Hinni K., Iraqui Houssaini I., Jacquet M., Jimenez A., Jonniaux J.-L., Karpfinger-Hartl L., Lanfranchi G., Lepingle A., Levesque H., Lyck R., Maftahi M., Mallet L., Maurer C.T.C., Messenguy F., Mewes H.-W., Moestl D., Nasr F., Nicaud J.-M., Niedenthal R.K., Pandolfo D., Pierard A., Piravandi E., Planta R.J., Pohl T.M., Purnelle B., Rebischung C., Remacha M.A., Revuelta J.L., Rinke M., Saiz J.E., Sartorello F., Scherens B., Sen-Gupta M., Soler-Mira A., Urbanus J.H.M., Valle G., Van Dyck L., Verhasselt P., Vierendeels F., Vissers S., Voet M., Volckaert G., Wach A., Wambutt R., Wedler H., Zollner A., Hani J.
    Nature 387:93-98(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "The URK1 gene of Saccharomyces cerevisiae encoding uridine kinase."
    Kern L.
    Nucleic Acids Res. 18:5279-5279(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 784-894.
    Strain: ATCC 28383 / FL100 / VTT C-80102.
  5. "Phosphate transport and sensing in Saccharomyces cerevisiae."
    Wykoff D.D., O'Shea E.K.
    Genetics 159:1491-1499(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Transcriptional regulation of phosphate-responsive genes in low-affinity phosphate-transporter-defective mutants in Saccharomyces cerevisiae."
    Auesukaree C., Homma T., Kaneko Y., Harashima S.
    Biochem. Biophys. Res. Commun. 306:843-850(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295 AND SER-311, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  9. "Pho91 is a vacuolar phosphate transporter that regulates phosphate and polyphosphate metabolism in Saccharomyces cerevisiae."
    Hurlimann H.C., Stadler-Waibel M., Werner T.P., Freimoser F.M.
    Mol. Biol. Cell 18:4438-4445(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  10. "The Rsp5 E3 ligase mediates turnover of low affinity phosphate transporters in Saccharomyces cerevisiae."
    Estrella L.A., Krishnamurthy S., Timme C.R., Hampsey M.
    J. Biol. Chem. 283:5327-5334(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: UBIQUITINATION BY RSP5.
  11. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-311 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-295; SER-311 AND SER-312, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Transport and signaling through the phosphate-binding site of the yeast Pho84 phosphate transceptor."
    Popova Y., Thayumanavan P., Lonati E., Agrochao M., Thevelein J.M.
    Proc. Natl. Acad. Sci. U.S.A. 107:2890-2895(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPHO91_YEAST
AccessioniPrimary (citable) accession number: P27514
Secondary accession number(s): D6W1I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: February 1, 1995
Last modified: July 6, 2016
This is version 140 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 4280 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XIV
    Yeast (Saccharomyces cerevisiae) chromosome XIV: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.