Reviewed, UniProtKB/Swiss-Prot P27498 (DYR_KLEAE)

Names and origin

Protein names

Recommended name:
    Dihydrofolate reductase
    EC=1.5.1.3

Gene names

Name:	folA
Synonyms:	atsR

Organism

Klebsiella aerogenes

Taxonomic identifier

28451 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Klebsiella

Protein attributes

Sequence length	159 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NADP or NADPH binding Inferred from electronic annotation. Source: InterPro dihydrofolate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 159	159	Dihydrofolate reductase		PRO_0000186392
Regions
	Domain	1 – 158	158	DHFR

Sequences

	Sequence		Length	Mass (Da)
	P27498-1 [UniParc]. Last modified August 1, 1992. Version 1. Checksum: BFDC5F9DA53CCE1E	FASTA	159	18,112
	10 20 30 40 50 60 MISLIAALRV DRVIGMENAM PWNLNEDLAW FKRNTLNKPV VMGRLTWESI GRPLPGRKNI 70 80 90 100 110 120 VISSKPGSDD RVQWVSSVEE AIAACGDVEE IMVIGGGRVY DEFLPKAQKL YLTHIDAEVE 130 140 150 GDTHFPDYDP DEWESVFSEF HDADAQNSHS YCFEILERR

References

[1]

"Cloning and nucleotide sequence of a negative regulator gene for Klebsiella aerogenes arylsulfatase synthesis and identification of the gene as folA." Azakami H., Sugino H., Murooka Y. J. Bacteriol. 174:2344-2351(1992) [PubMed: 1551851] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: W70.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D10358 Genomic DNA. Translation: BAA01187.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHI based on UniProtKB P00379.
SMR	P27498. Positions 1-159.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.5.1.3. 366.
Family and domain databases
InterPro	IPR012259. DHFR. IPR001796. DHFR_reg. IPR017925. Dihydrofolate_reductase_CS. [Graphical view]
PANTHER	PTHR11549:SF1. DHFR. 1 hit.
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_KLEAE

Accession

Primary (citable) accession number: P27498

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	August 1, 1992
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)