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Protein

Calmodulin-like protein 3

Gene

CALML3

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

May function as a specific light chain of unconventional myosin-10 (MYO10), also enhances MYO10 translation, possibly by acting as a chaperone for the emerging MYO10 heavy chain protein. May compete with calmodulin by binding, with different affinities, to cellular substrates.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi21 – 32121Add
BLAST
Calcium bindingi57 – 68122Add
BLAST
Calcium bindingi94 – 105123Add
BLAST
Calcium bindingi130 – 141124Add
BLAST

GO - Molecular functioni

  1. calcium ion binding Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calmodulin-like protein 3
Alternative name(s):
CaM-like protein
Short name:
CLP
Calmodulin-related protein NB-1
Gene namesi
Name:CALML3
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 10

Organism-specific databases

HGNCiHGNC:1452. CALML3.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: UniProtKB
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26044.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 149149Calmodulin-like protein 3PRO_0000073547Add
BLAST

Proteomic databases

MaxQBiP27482.
PaxDbiP27482.
PeptideAtlasiP27482.
PRIDEiP27482.

PTM databases

PhosphoSiteiP27482.

Expressioni

Tissue specificityi

Expressed in normal mammary, prostate, cervical, and epidermal tissues. It is greatly reduced or undetectable in transformed cells.1 Publication

Inductioni

By TGFB1.1 Publication

Gene expression databases

BgeeiP27482.
CleanExiHS_CALML3.
GenevestigatoriP27482.

Organism-specific databases

HPAiCAB010070.
HPA044999.

Interactioni

Subunit structurei

Interacts with MYO10, the interaction is calcium-dependent and essential for MYO10 function in filopodial extension.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
MYO10Q9HD672EBI-747537,EBI-307061

Protein-protein interaction databases

BioGridi107261. 28 interactions.
IntActiP27482. 8 interactions.
MINTiMINT-1440064.
STRINGi9606.ENSP00000315299.

Structurei

Secondary structure

1
149
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi7 – 2014Combined sources
Beta strandi25 – 284Combined sources
Helixi30 – 3910Combined sources
Helixi46 – 549Combined sources
Beta strandi61 – 655Combined sources
Helixi66 – 9328Combined sources
Beta strandi98 – 1014Combined sources
Helixi103 – 11210Combined sources
Helixi119 – 12911Combined sources
Beta strandi133 – 1386Combined sources
Helixi139 – 1479Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GGZX-ray1.50A2-149[»]
ProteinModelPortaliP27482.
SMRiP27482. Positions 5-148.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP27482.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini8 – 4336EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini44 – 7936EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini81 – 11636EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini117 – 14933EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the calmodulin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP27482.
KOiK02183.
OMAiCITTQEL.
OrthoDBiEOG7F7WBV.
PhylomeDBiP27482.
TreeFamiTF300912.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 3 hits.
PF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P27482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADQLTEEQV TEFKEAFSLF DKDGDGCITT RELGTVMRSL GQNPTEAELR
60 70 80 90 100
DMMSEIDRDG NGTVDFPEFL GMMARKMKDT DNEEEIREAF RVFDKDGNGF
110 120 130 140
VSAAELRHVM TRLGEKLSDE EVDEMIRAAD TDGDGQVNYE EFVRVLVSK
Length:149
Mass (Da):16,891
Last modified:January 23, 2007 - v2
Checksum:i1AB883E8ED4D263D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58026 mRNA. Translation: AAA36356.1.
X13461 Genomic DNA. Translation: CAA31809.1.
AL732437 Genomic DNA. Translation: CAI11029.1.
AK313934 mRNA. Translation: BAG36653.1.
CH471072 Genomic DNA. Translation: EAW86443.1.
BC031889 mRNA. Translation: AAH31889.1.
CCDSiCCDS7069.1.
PIRiA38278. MCHUNB.
RefSeqiNP_005176.1. NM_005185.3.
UniGeneiHs.239600.

Genome annotation databases

EnsembliENST00000315238; ENSP00000315299; ENSG00000178363.
GeneIDi810.
KEGGihsa:810.
UCSCiuc001iie.1. human.

Polymorphism databases

DMDMi115502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M58026 mRNA. Translation: AAA36356.1.
X13461 Genomic DNA. Translation: CAA31809.1.
AL732437 Genomic DNA. Translation: CAI11029.1.
AK313934 mRNA. Translation: BAG36653.1.
CH471072 Genomic DNA. Translation: EAW86443.1.
BC031889 mRNA. Translation: AAH31889.1.
CCDSiCCDS7069.1.
PIRiA38278. MCHUNB.
RefSeqiNP_005176.1. NM_005185.3.
UniGeneiHs.239600.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1GGZX-ray1.50A2-149[»]
ProteinModelPortaliP27482.
SMRiP27482. Positions 5-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107261. 28 interactions.
IntActiP27482. 8 interactions.
MINTiMINT-1440064.
STRINGi9606.ENSP00000315299.

PTM databases

PhosphoSiteiP27482.

Polymorphism databases

DMDMi115502.

Proteomic databases

MaxQBiP27482.
PaxDbiP27482.
PeptideAtlasiP27482.
PRIDEiP27482.

Protocols and materials databases

DNASUi810.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000315238; ENSP00000315299; ENSG00000178363.
GeneIDi810.
KEGGihsa:810.
UCSCiuc001iie.1. human.

Organism-specific databases

CTDi810.
GeneCardsiGC10P005556.
HGNCiHGNC:1452. CALML3.
HPAiCAB010070.
HPA044999.
MIMi114184. gene.
neXtProtiNX_P27482.
PharmGKBiPA26044.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG5126.
GeneTreeiENSGT00760000118901.
HOGENOMiHOG000233018.
HOVERGENiHBG012180.
InParanoidiP27482.
KOiK02183.
OMAiCITTQEL.
OrthoDBiEOG7F7WBV.
PhylomeDBiP27482.
TreeFamiTF300912.

Miscellaneous databases

ChiTaRSiCALML3. human.
EvolutionaryTraceiP27482.
GeneWikiiCALML3.
GenomeRNAii810.
NextBioi3288.
PROiP27482.
SOURCEiSearch...

Gene expression databases

BgeeiP27482.
CleanExiHS_CALML3.
GenevestigatoriP27482.

Family and domain databases

Gene3Di1.10.238.10. 2 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF00036. EF-hand_1. 3 hits.
PF13405. EF-hand_6. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 4 hits.
[Graphical view]
PROSITEiPS00018. EF_HAND_1. 4 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Down-regulation of a calmodulin-related gene during transformation of human mammary epithelial cells."
    Yaswen P., Smoll A., Peehl D.M., Trask D.K., Sager R., Stampfer M.R.
    Proc. Natl. Acad. Sci. U.S.A. 87:7360-7364(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY TGFB1.
  2. "Characterization of an intronless human calmodulin-like pseudogene."
    Koller M., Strehler E.E.
    FEBS Lett. 239:121-128(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. Koller M.
    Submitted (DEC-1991) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  4. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Esophagus.
  5. "The DNA sequence and comparative analysis of human chromosome 10."
    Deloukas P., Earthrowl M.E., Grafham D.V., Rubenfield M., French L., Steward C.A., Sims S.K., Jones M.C., Searle S., Scott C., Howe K., Hunt S.E., Andrews T.D., Gilbert J.G.R., Swarbreck D., Ashurst J.L., Taylor A., Battles J.
    , Bird C.P., Ainscough R., Almeida J.P., Ashwell R.I.S., Ambrose K.D., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Bates K., Beasley H., Bray-Allen S., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Cahill P., Camire D., Carter N.P., Chapman J.C., Clark S.Y., Clarke G., Clee C.M., Clegg S., Corby N., Coulson A., Dhami P., Dutta I., Dunn M., Faulkner L., Frankish A., Frankland J.A., Garner P., Garnett J., Gribble S., Griffiths C., Grocock R., Gustafson E., Hammond S., Harley J.L., Hart E., Heath P.D., Ho T.P., Hopkins B., Horne J., Howden P.J., Huckle E., Hynds C., Johnson C., Johnson D., Kana A., Kay M., Kimberley A.M., Kershaw J.K., Kokkinaki M., Laird G.K., Lawlor S., Lee H.M., Leongamornlert D.A., Laird G., Lloyd C., Lloyd D.M., Loveland J., Lovell J., McLaren S., McLay K.E., McMurray A., Mashreghi-Mohammadi M., Matthews L., Milne S., Nickerson T., Nguyen M., Overton-Larty E., Palmer S.A., Pearce A.V., Peck A.I., Pelan S., Phillimore B., Porter K., Rice C.M., Rogosin A., Ross M.T., Sarafidou T., Sehra H.K., Shownkeen R., Skuce C.D., Smith M., Standring L., Sycamore N., Tester J., Thorpe A., Torcasso W., Tracey A., Tromans A., Tsolas J., Wall M., Walsh J., Wang H., Weinstock K., West A.P., Willey D.L., Whitehead S.L., Wilming L., Wray P.W., Young L., Chen Y., Lovering R.C., Moschonas N.K., Siebert R., Fechtel K., Bentley D., Durbin R.M., Hubbard T., Doucette-Stamm L., Beck S., Smith D.R., Rogers J.
    Nature 429:375-381(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Skin.
  8. "Characterization of the human calmodulin-like protein expressed in Escherichia coli."
    Rhyner J.A., Koller M., Durussel-Gerber I., Cox J.A., Strehler E.E.
    Biochemistry 31:12826-12832(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  9. "The tumor-sensitive calmodulin-like protein is a specific light chain of human unconventional myosin X."
    Rogers M.S., Strehler E.E.
    J. Biol. Chem. 276:12182-12189(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH MYO10.
  10. "Calmodulin-like protein enhances myosin-10 translation."
    Bennett R.D., Strehler E.E.
    Biochem. Biophys. Res. Commun. 369:654-659(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  11. "Crystal structure of human calmodulin-like protein: insights into its functional role."
    Han B.G., Han M., Sui H., Yaswen P., Walian P.J., Jap B.K.
    FEBS Lett. 521:24-30(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

Entry informationi

Entry nameiCALL3_HUMAN
AccessioniPrimary (citable) accession number: P27482
Secondary accession number(s): B2R9V6, Q5SQI4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 1, 1992
Last sequence update: January 23, 2007
Last modified: February 4, 2015
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Binds four calcium ions.

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 10
    Human chromosome 10: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.