Reviewed, UniProtKB/Swiss-Prot P27479 (LOX12_BOVIN)

Names and origin

Protein names

Recommended name:
    Arachidonate 12-lipoxygenase, 12S-type
      Short name=12-LOX
    EC=1.13.11.31

Gene names

Name:

ALOX12

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	663 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Oxygenase and 14,15-leukotriene A4 synthase activity. Ref.1
Catalytic activity	Arachidonate + O2 = (5Z,8Z,10E,14Z)-(12S)-12-hydroperoxyicosa-5,8,10,14-tetraenoate.
Cofactor	Binds 1 iron ion per subunit.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Subcellular location	Cytoplasm.
Tissue specificity	Detected in tracheal epithelium. Ref.1
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Cellular component	Cytoplasm
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	arachidonate 12-lipoxygenase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 663	662	Arachidonate 12-lipoxygenase, 12S-type		PRO_0000220681
Regions
	Domain	2 – 115	114	PLAT
	Domain	116 – 663	548	Lipoxygenase
Sites
	Metal binding	361	1	Iron; catalytic By similarity
	Metal binding	366	1	Iron; catalytic By similarity
	Metal binding	541	1	Iron; catalytic By similarity
	Metal binding	663	1	Iron; via carboxylate; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P27479-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 58063351F14E15D4	FASTA	663	75,041
	10 20 30 40 50 60 MGLYRVRVST GSSFCAGSNN QVHLWLVGEH GEAALGWAVR PARGKEVEFQ VDVSEYLGRL 70 80 90 100 110 120 LFVKLRKRHL LSDDAWFCNW ISVQGPGASG NEFRFPCYRW VEGDGILSLP EGTGRTVVDD 130 140 150 160 170 180 PQGLFKKHRE EELAERRKLY RWGNWKDGLI LNIAGATIND LPVDERFLED KRIDFEASLT 190 200 210 220 230 240 KGLADLAIKD SLNILTCWKS LDDFNRIFWC GQSKLAERVR DSWKEDALFG YQFLNGTNPM 250 260 270 280 290 300 LLRRSVRLPA RLEFPPGMGE LQAELEKELQ QGTLFEADFS LLMGIKANVI LCTQQYVAAP 310 320 330 340 350 360 LVMLKLQPDG KLLPMAIQLQ LPHKGSPPPP LFLPTDPPMT WLLAKCWVRS SDFQLHELHS 370 380 390 400 410 420 HLLRGHLVAE VIAVATMRCL PSIHPMFKLL IPHLRYTMEI NIRARTGLVS DSGVFDQVVS 430 440 450 460 470 480 TGGGGHVELL QRAGAFLTYS SFCPPDDLAD RGLLGVKSSF YAQDALRLWE ILSRYVEGIV 490 500 510 520 530 540 SLHYKTDESV RDDIELQAWC RDITEIGLLG AQDRGFPVTL QSKDQLCHFV TMCIFTCTGQ 550 560 570 580 590 600 HSSTHLGQLD WYSWVPNAPC TMRLPPPTTK DVTLEKVMAT LPNFHQASLQ MSITWQLGRR 610 620 630 640 650 660 QPIMVALGQH EEEYFSGPEP KAVLKKFREE LAALEKDIEI RNAQLDWPYE YLRPSLVENS VAI

References

[1]	"Cloning and expression of an airway epithelial 12-lipoxygenase." de Marzo N., Sloan D.L., Dicharry S., Highland E., Sigal E. Am. J. Physiol. 262:L198-L207(1992) [PubMed: 1539676] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Tracheal epithelium.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M81320 mRNA. Translation: AAA30346.1. M62516 mRNA. Translation: AAC41614.1. S96247 mRNA. Translation: AAB21522.2. Sequence problems.
IPI	IPI00697083.
PIR	S32825.
RefSeq	NP_776926.1.
UniGene	Bt.2598
3D structure databases
HSSP	HSSP built from PDB template 1LOX based on UniProtKB P12530.
SMR	P27479. Positions 2-663.
ModBase	Search...
Protein-protein interaction databases
STRING	P27479.
Genome annotation databases
Ensembl	ENSBTAT00000015911; ENSBTAP00000015911; ENSBTAG00000011990; Bos taurus. [Genome view]
GeneID	282139.
KEGG	bta:282139.
Organism-specific databases
CTD	282139.
Phylogenomic databases
HOVERGEN	P27479.
Enzyme and pathway databases
BRENDA	1.13.11.31. 251.
Family and domain databases
InterPro	IPR000907. LipOase. IPR013819. LipOase_C. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LOX12_BOVIN

Accession

Primary (citable) accession number: P27479

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 1, 1992
Last sequence update:	January 23, 2007
Last modified:	September 1, 2009
This is version 75 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)